LOCUS       pBAD/His-DiB1           4492 bp ds-DNA     circular     12-MAR-2021
DEFINITION  .
KEYWORDS    "marker:AmpR" "ref:pmid\:21765402"                                 
FEATURES             Location/Qualifiers
     CDS             1..1
                     /label="iRFP713"
                     /ApEinfo_revcolor=#a0003c
                     /ApEinfo_fwdcolor=#a0003c
     terminator      212..298
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      390..417
                     /label="rrnB T2 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        437..528
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             529..597
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             598..1389
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      1560..2148
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2334..2474
                     /label="bom"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(2738..3616)
                     /label="araC"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     promoter        3643..3927
                     /label="araBAD promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             3962..4492
                     /label="Translation 3962-4492"
     CDS             3974..3991
                     /label="6xHis"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3992..4492
                     /label="DiB1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 tagaagcttg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
       61 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
      121 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
      181 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      241 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
      301 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
      361 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
      421 gtttctacaa actctttttg tttatttttc taaatacatt caaatatgta tccgctcatg
      481 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      541 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      601 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      661 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      721 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
      781 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      841 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      901 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
      961 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1021 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1081 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1141 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1201 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1261 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1321 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1381 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1441 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1501 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1561 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1621 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1681 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1741 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1801 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1861 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1921 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1981 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2041 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2101 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2161 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2221 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2281 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2341 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     2401 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     2461 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     2521 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     2581 ggttttcacc gtcatcaccg aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga
     2641 agcggcatgc ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct
     2701 actccgtcaa gccgtcaatt gtctgattcg ttaccaatta tgacaacttg acggctacat
     2761 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt
     2821 taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga
     2881 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc
     2941 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca
     3001 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga
     3061 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg
     3121 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc
     3181 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt
     3241 gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt
     3301 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg
     3361 gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc
     3421 aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa
     3481 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa
     3541 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat
     3601 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat
     3661 attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg
     3721 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc
     3781 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca
     3841 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt
     3901 tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctaacagg aggaattaac
     3961 catggggggt tctcatcatc atcatcatca tCTCGAATCA ACCAGCTTGT ACAAGAAATC
     4021 CAGCTCCACG CCGCCGCGTG GCGTGACCGT AGTAAATAAT TTCGACTGCA AACGTTATCT
     4081 CGGTACCTGG TATGAGATTG CCCGTTTTGA TCACCGCTTT GAACGTGGAC TGGAAAAAGT
     4141 CACCGCAACA TACAGCCTGC GTGATGACGG CGGCCTGAAT GTCATTAATA AAGGCTATAA
     4201 CCCTGACAGA GGAATGTGGC AGCAGAGTGA AGGGAAAGCG TACTTTACCG GCGCACCAAC
     4261 TCGCGCTGCG CTGAAAGTGT CATTCTTTGG TCCTTTCTAT GGCGGTTATA ACGTTATTGC
     4321 ACTCGATCGG GAATACCGCC ATGCGCTGGT TTGCGGCCCG GACCGCGACT ACCTGTGGAT
     4381 CAACTCCCGC ACGCCAACCA TTTCTGACGA AGTGAAACAG GAGATGCTGG CAGTCGCGAC
     4441 CCGGGAAGGG TTTGATGTCA GTAAATTTAT TTGGGTACAG CAGCCTGGTA GT
//