LOCUS       Exported                5648 bp ds-DNA     circular SYN 24-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Mayra Sauer
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Feb 24, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      complement(864..891)
                     /label=rrnB T2
     terminator      complement(1023..1066)
                     /label=rrnB T1
     primer_bind     1132..1149
                     /label=M13-fwd
     primer_bind     1152..1172
                     /label=Seq-pENTR-SfiI_fwd
     misc_recomb     1166..1261
                     /label=AttL1
     misc_recomb     1241..1261
                     /label=attB1
     misc_feature    1241..1261
                     /label=Gateway recomb1
     misc_feature    1265..1272
                     /label=Sfi-A
     misc_feature    4121..4125
                     /label=Sfi-B
     misc_recomb     complement(4126..4146)
                     /label=attB2
     misc_feature    complement(4126..4146)
                     /label=Gateway Recomb2
     primer_bind     complement(4219..4239)
                     /label=Seq_pENTR-SfiI_rev
     primer_bind     complement(4239..4258)
                     /label=T7
     primer_bind     complement(4259..4279)
                     /label=M13-rev
     primer_bind     complement(4288..4307)
                     /label=P4-p1R_blunt rev
     CDS             4491..5501
                     /label=aadA
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tcctctaaag attccaattt tttaaaccga
     1321 gtccggggtt ctattgtaaa tgcccgaata gaaccctgtt caagtggttt gtccactgat
     1381 gtcgtcttta gggcatttga catctgcaac tataaggcta aggttgctgg tattggaaaa
     1441 tactacaaga ctaatacttg taggtttgta gaattagatg accaagggca tcatttagac
     1501 tcctattttg tcgttaagag gcatactatg gagaattatg aactagagaa gcactgttac
     1561 gacttgttac gtgactgtga tgctgtagct ccccatgatt tcttcatctt tgatgtagac
     1621 aaagttaaaa cacctcatat tgtacgtcag cgtttaactg agtacactat gatggatctt
     1681 gtatatgccc tgaggcactt tgatcaaaat agcgaagtgc ttaaggctat cttagtgaag
     1741 tatggttgct gtgatgttac ctactttgaa aataaactct ggtttgattt tgttgaaaat
     1801 cccagtgtta ttggtgttta tcataaactt ggagaacgtg tacgccaagc tatcttaaac
     1861 actgttaaat tttgtgacca catggtcaag gctggtttag tcggtgtgct cacactagac
     1921 aaccaggacc ttaatggcaa gtggtatgat tttggtgact tcgtaatcac tcaacctggt
     1981 tcaggagtag ctatagttga tagctactat tcttatttga tgcctgtgct ctcaatgacc
     2041 gattgtctgg ccgctgagac acatagggat tgtgatttta ataaaccact cattgagtgg
     2101 ccacttactg agtatgattt tactgattat aaggtacaac tctttgagaa gtactttaaa
     2161 tattgggatc agacgtatca cgcaaattgc gttaattgta ctgatgaccg ttgtgtgtta
     2221 cattgtgcta atttcaatgt attgtttgct atgaccatgc ctaagacttg tttcggaccc
     2281 atagtccgaa agatctttgt tgatggcgtg ccatttgtag tatcttgtgg ttatcactac
     2341 aaagaattag gtttagtcat gaatatggat gttagtctcc atagacatag gctctctctt
     2401 aaggagttga tgatgtatgc cgctgatcca gccatgcaca ttgcctcctc taacgctttt
     2461 cttgatttga ggacatcatg ttttagtgtc gctgcactta caactggttt gacttttcaa
     2521 actgtgcggc ctggcaattt taaccaagac ttctatgatt tcgtggtatc taaaggtttc
     2581 tttaaggagg gctcttcagt gacgctcaaa cattttttct ttgctcaaga tggtaatgct
     2641 gctattacag attataatta ctattcttat aatctgccta ctatgtgtga catcaaacaa
     2701 atgttgttct gcatggaagt tgtaaacaag tacttcgaaa tctatgacgg tggttgtctt
     2761 aatgcttctg aagtggttgt taataattta gacaagagtg ctggccatcc ttttaataag
     2821 tttggcaaag ctcgtgtcta ttatgagagc atgtcttacc aggagcaaga tgaacttttt
     2881 gccatgacaa agcgtaacgt cattcctacc atgactcaaa tgaatctaaa atatgctatt
     2941 agtgctaaga atagagctcg cactgttgca ggcgtgtcca tacttagcac aatgactaat
     3001 cgccagtacc atcagaaaat gcttaagtcc atggctgcaa ctcgtggagc gacttgcgtc
     3061 attggtacta caaagttcta cggtggctgg gatttcatgc ttaaaacatt gtacaaagat
     3121 gttgataatc cgcatcttat gggttgggat taccctaagt gtgatagagc tatgcctaat
     3181 atgtgtagaa tcttcgcttc actcatatta gctcgtaaac atggcacttg ttgtactaca
     3241 agggacagat tttatcgctt ggcaaatgag tgtgctcagg tgctaagcga atatgttcta
     3301 tgtggtggtg gttactacgt caaacctgga ggtaccagta gcggagatgc caccactgca
     3361 tatgccaata gtgtctttaa cattttgcag gcgacaactg ctaatgtcag tgcacttatg
     3421 ggtgctaatg gcaacaagat tgttgacaaa gaagttaaag acatgcagtt tgatttgtat
     3481 gtcaatgttt acaggagcac tagcccagac cccaaatttg ttgataaata ctatgctttt
     3541 cttaataagc acttttctat gatgatactg tctgatgacg gtgtcgtttg ctataatagt
     3601 gattatgcag ctaagggtta cattgctgga atacagaatt ttaaggaaac gctgtattat
     3661 cagaacaatg tctttatgtc tgaagctaaa tgctgggtgg aaaccgatct gaagaaaggg
     3721 ccacatgaat tctgttcaca gcatacgctt tatattaagg atggcgacga tggttacttc
     3781 cttccttatc cagacccttc aagaattttg tctgccggtt gctttgtaga tgatatcgtt
     3841 aagactgacg gtacactcat ggtagagcgg tttgtgtctt tggctataga tgcttaccct
     3901 ctcacaaagc atgaagatat agaataccag aatgtattct gggtctactt acagtatata
     3961 gaaaaactgt ataaagacct tacaggacac atgcttgaca gttattctgt catgctatgt
     4021 ggtgataatt ctgctaagtt ttgggaagag gcattctata gagatctcta tagttcgcct
     4081 accactttgc agcttaatta agacgcgatc gcggcctcat gggcccagct ttcttgtaca
     4141 aagttggcat tataaaaaat aattgctcat caatttgttg caacgaacag gtcactatca
     4201 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     4261 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     4321 cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga
     4381 cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     4441 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     4501 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     4561 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     4621 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     4681 gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga
     4741 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     4801 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     4861 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     4921 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     4981 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     5041 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     5101 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     5161 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     5221 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     5281 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     5341 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     5401 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     5461 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     5521 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     5581 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5641 aaccaccg
//
