LOCUS       Exported                6905 bp ds-DNA     circular SYN 22-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Mayra Sauer
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 22, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             complement(4855..4866)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tccatacact cagtgtttct actgatgttc
     1321 ttgttaacac ctacagaaag ttacgttgat gtagggccag attctgttaa gtctgcttgt
     1381 attgaggttg atatacaaca gactttcttt gataaaactt ggcctaggcc aattgatgtt
     1441 tctaaggctg acggtattat ataccctcaa ggccgtacat attctaacat aactatcact
     1501 tatcaaggtc tttttcccta tcagggagac catggtgata tgtatgttta ctctgcagga
     1561 catgctacag gcacaactcc acaaaagttg tttgtagcta actattctca ggacgtcaaa
     1621 cagtttgcta atgggtttgt cgtccgtata ggagcagctg ccaattccac tggcactgtt
     1681 attattagcc catctaccag cgctactata cgaaaaattt accctgcttt tatgctgggt
     1741 tcttcagttg gtaatttctc agatggtaaa atgggccgct tcttcaatca tactctagtt
     1801 cttttgcccg atggatgtgg cactttactt agagcttttt attgtattct agagcctcgc
     1861 tctggaaatc attgtcctgc tggcaattcc tatacttctt ttgccactta tcacactcct
     1921 gcaacagatt gttctgatgg caattacaat cgtaatgcca gtctgaactc ttttaaggag
     1981 tattttaatt tacgtaactg cacctttatg tacacttata acattaccga agatgagatt
     2041 ttagagtggt ttggcattac acaaactgct caaggtgttc acctcttctc atctcggtat
     2101 gttgatttgt acggcggcaa tatgtttcaa tttgccacct tgcctgttta tgatactatt
     2161 aagtattatt ctatcattcc tcacagtatt cgttctatcc aaagtgatag aaaagcttgg
     2221 gctgccttct acgtatataa acttcaaccg ttaactttcc tgttggattt ttctgttgat
     2281 ggttatatac gcagagctat agactgtggt tttaatgatt tgtcacaact ccactgctca
     2341 tatgaatcct tcgatgttga atctggagtt tattcagttt cgtctttcga agcaaaacct
     2401 tctggctcag ttgtggaaca ggctgaaggt gttgaatgtg atttttcacc tcttctgtct
     2461 ggcacacctc ctcaggttta taatttcaag cgtttggttt ttaccaattg caattataat
     2521 cttaccaaat tgctttcact tttttctgtg aatgatttta cttgtagtca aatatctcca
     2581 gcagcaattg ctagcaactg ttattcttca ctgattttgg attacttttc atacccactt
     2641 agtatgaaat ccgatctcag tgttagttct gctggtccaa tatcccagtt taattataaa
     2701 cagtcctttt ctaatcccac atgtttgatt ttagcgactg ttcctcataa ccttactact
     2761 attactaagc ctcttaagta cagctatatt aacaagtgct ctcgtcttct ttctgatgat
     2821 cgtactgaag tacctcagtt agtgaacgct aatcaatact caccctgtgt atccattgtc
     2881 ccatccactg tgtgggaaga cggtgattat tataggaaac aactatctcc acttgaaggt
     2941 ggtggctggc ttgttgctag tggctcaact gttgccatga ctgagcaatt acagatgggc
     3001 tttggtatta cagttcaata tggtacagac accaatagtg tttgccccaa gcttgaattt
     3061 gctaatgaca caaaaattgc ctctcaatta ggcaattgcg tggaatattc cctctatggt
     3121 gtttcgggcc gtggtgtttt tcagaattgc acagctgtag gtgttcgaca gcagcgcttt
     3181 gtttatgatg cgtaccagaa tttagttggc tattattctg atgatggcaa ctactactgt
     3241 ttgcgtgctt gtgttagtgt tcctgtttct gtcatctatg ataaagaaac taaaacccac
     3301 gctactctat ttggtagtgt tgcatgtgaa cacatttctt ctaccatgtc tcaatactcc
     3361 cgttctacgc gatcaatgct taaacggcga gattctacat atggccccct tcagacacct
     3421 gttggttgtg tcctaggact tgttaattcc tctttgttcg tagaggactg caagttgcct
     3481 cttggtcaat ctctctgtgc tcttcctgac acacctagta ctctcacacc tcgcagtgtg
     3541 cgctctgttc caggtgaaat gcgcttggca tccattgctt ttaatcatcc tattcaggtt
     3601 gatcaactta atagtagtta ttttaaatta agtataccca ctaatttttc ctttggtgtg
     3661 actcaggagt acattcagac aaccattcag aaagttactg ttgattgtaa acagtacgtt
     3721 tgcaatggtt tccagaagtg tgagcaatta ctgcgcgagt atggccagtt ttgttccaaa
     3781 ataaaccagg ctctccatgg tgccaattta cgccaggatg attctgtacg taatttgttt
     3841 gcgagcgtga aaagctctca atcatctcct atcataccag gttttggagg tgactttaat
     3901 ttgacacttc tagaacctgt ttctatatct actggcagtc gtagtgcacg tagtgctatt
     3961 gaggatttgc tatttgacaa agtcactata gctgatcctg gttatatgca aggttacgat
     4021 gattgcatgc agcaaggtcc agcatcagct cgtgatctta tttgtgctca atatgtggct
     4081 ggttacaaag tattacctcc tcttatggat gttaatatgg aagccgcgta tacttcatct
     4141 ttgcttggca gcatagcagg tgttggctgg actgctggct tatcctcctt tgctgctatt
     4201 ccatttgcac agagtatctt ttataggtta aacggtgttg gcattactca acaggttctt
     4261 tcagagaacc aaaagcttat tgccaataag tttaatcagg ctctgggagc tatgcaaaca
     4321 ggcttcacta caactaatga agcttttcag aaggttcagg atgctgtgaa caacaatgca
     4381 caggctctat ccaaattagc tagcgagcta tctaatactt ttggtgctat ttccgcctct
     4441 attggagaca tcatacaacg tcttgatgtt ctcgaacagg acgcccaaat agacagactt
     4501 attaatggcc gtttgacaac actaaatgct tttgttgcac agcagcttgt tcgttccgaa
     4561 tcagctgctc tttccgctca attggctaaa gataaagtca atgagtgtgt caaggcacaa
     4621 tccaagcgtt ctggattttg cggtcaaggc acacatatag tgtcctttgt tgtaaatgcc
     4681 cctaatggcc tttacttcat gcatgttggt tattacccta gcaaccacat tgaggttgtt
     4741 tctgcttatg gtctttgcga tgcagctaac cctactaatt gtatagcccc tgttaatggc
     4801 tactttatta aaactaataa cactaggatt gttgatgagt ggtcatatac tggctcgtcc
     4861 ttctatgcac ctgagcccat tacctccctt aatactaagt atgttgcacc acaggtgaca
     4921 taccaaaaca tttctactaa cctccctcct cctcttctcg gcaattccac cgggattgac
     4981 ttccaagatg agttggatga gtttttcaaa aatgttagca ccagtatacc taattttggt
     5041 tccctaacac agattaatac tacattactc gatcttacct acgagatgtt gtctcttcaa
     5101 caagttgtta aagcccttaa tgagtcttac atagacctta aagagcttgg caattatact
     5161 tattacaaca aatggccgtg gtacatttgg cttggtttca ttgctgggct tgttgcctta
     5221 gctctatgcg tcttcttcat actgtgctgc actggttgtg gcacaaactg tatgggaaaa
     5281 cttaagtgta atcgttgttg tgatagatac gaggaatacg acctcgagcc gcataaggtt
     5341 catgttcacc ttaattaaga cgcgatcgcg gcctcatggg cccagctttc ttgtacaaag
     5401 ttggcattat aaaaaataat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc
     5461 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     5521 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     5581 aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt
     5641 cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     5701 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     5761 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     5821 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     5881 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     5941 gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc
     6001 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     6061 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     6121 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     6181 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     6241 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     6301 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     6361 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     6421 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     6481 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     6541 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     6601 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     6661 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     6721 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     6781 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     6841 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6901 caccg
//
