LOCUS       Exported                6611 bp ds-DNA     circular SYN 23-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Mayra Sauer
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 23, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    5005..5016
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tcctttattt tcttattatt tcttactctc
     1321 actagtggta gtgaccttga ccggtgcacc acttttgatg atgttcaagc tcctaattac
     1381 actcaacata cttcatctat gaggggggtt tactatcctg atgaaatttt tagatcagac
     1441 actctttatt taactcagga tttatttctt ccattttatt ctaatgttac agggtttcat
     1501 actattaatc atacgtttgg caaccctgtc atacctttta aggatggtat ttattttgct
     1561 gccacagaga aatcaaatgt tgtccgtggt tgggtttttg gttctaccat gaacaacaag
     1621 tcacagtcgg tgattattat taacaattct actaatgttg ttatacgagc atgtaacttt
     1681 gaattgtgtg acaacccttt ctttgctgtt tctaaaccca tgggtacaca gacacatact
     1741 atgatattcg ataatgcatt taattgcact ttcgagtaca tatctgatgc cttttcgctt
     1801 gatgtttcag aaaagtcagg taattttaaa cacttacgag agtttgtgtt taaaaataaa
     1861 gatgggtttc tctatgttta taagggctat caacctatag atgtagttcg tgatctacct
     1921 tctggtttta acactttgaa acctattttt aagttgcctc ttggtattaa cattacaaat
     1981 tttagagcca ttcttacagc cttttcacct gctcaagaca tttggggcac gtcagctgca
     2041 gcctattttg ttggctattt aaagccaact acatttatgc tcaagtatga tgaaaatggt
     2101 acaatcacag atgctgttga ttgttctcaa aatccacttg ctgaactcaa atgctctgtt
     2161 aagagctttg agattgacaa aggaatttac cagacctcta atttcagggt tgttccctca
     2221 ggagatgttg tgagattccc taatattaca aacttgtgtc cttttggaga ggtttttaat
     2281 gctactaaat tcccttctgt ctatgcatgg gagagaaaaa aaatttctaa ttgtgttgct
     2341 gattactctg tgctctacaa ctcaacattt ttttcaacct ttaagtgcta tggcgtttct
     2401 gccactaagt tgaatgatct ttgcttctcc aatgtctatg cagattcttt tgtagtcaag
     2461 ggagatgatg taagacaaat agcgccagga caaactggtg ttattgctga ttataattat
     2521 aaattgccag atgatttcat gggttgtgtc cttgcttgga atactaggaa cattgatgct
     2581 acttcaactg gtaattataa ttataaatat aggtatctta gacatggcaa gcttaggccc
     2641 tttgagagag acatatctaa tgtgcctttc tcccctgatg gcaaaccttg caccccacct
     2701 gctcttaatt gttattggcc attaaatgat tatggttttt acaccactac tggcattggc
     2761 taccaacctt acagagttgt agtactttct tttgaacttt taaatgcacc ggccacggtt
     2821 tgtggaccaa aattatccac tgaccttatt aagaaccagt gtgtcaattt taattttaat
     2881 ggactcactg gtactggtgt gttaactcct tcttcaaaga gatttcaacc atttcaacaa
     2941 tttggccgtg atgtttctga tttcactgat tccgttcgag atcctaaaac atctgaaata
     3001 ttagacattt caccttgcgc ttttgggggt gtaagtgtaa ttacacctgg aacaaatgct
     3061 tcatctgaag ttgctgttct atatcaagat gttaactgca ctgatgtttc tacagcaatt
     3121 catgcagatc aactcacacc agcttggcgc atatattcta ctggaaacaa tgtattccag
     3181 actcaagcag gctgtcttat aggagctgag catgtcgaca cttcttatga gtgcgacatt
     3241 cctattggag ctggcatttg tgctagttac catacagttt ctttattacg tagtactagc
     3301 caaaaatcta ttgtggctta tactatgtct ttaggtgctg atagttcaat tgcttactct
     3361 aataacacca ttgctatacc tactaacttt tcaattagca ttactacaga agtaatgcct
     3421 gtttctatgg ctaaaacctc cgtagattgt aatatgtaca tctgcggaga ttctactgaa
     3481 tgtgctaatt tgcttctcca atatggtagc ttttgcacac aactaaatcg tgcactctca
     3541 ggtattgctg ctgaacagga tcgcaacaca cgtgaagtgt tcgctcaagt caaacaaatg
     3601 tacaaaaccc caactttgaa atattttggt ggttttaatt tttcacaaat attacctgac
     3661 cctctaaagc caactaagag gtcttttatt gaggacttgc tctttaataa ggtgacactc
     3721 gctgatgctg gcttcatgaa gcaatatggc gaatgcctag gtgatattaa tgctagagat
     3781 ctcatttgtg cgcagaagtt caatggactt acagtgttgc cacctctgct cactgatgat
     3841 atgattgctg cctacactgc tgctctagtt agtggtactg ccactgctgg atggacattt
     3901 ggtgctggcg ctgctcttca aatacctttt gctatgcaaa tggcatatag gttcaatggc
     3961 attggagtta cccaaaatgt tctctatgag aaccaaaaac aaatcgccaa ccaatttaac
     4021 aaggcgatta gtcaaattca agaatcactt acaacaacat caactgcatt gggcaagctg
     4081 caagacgttg ttaaccagaa tgctcaagca ttaaacacac ttgttaaaca acttagctct
     4141 aattttggtg caatttcaag tgtgctaaat gatatccttt cgcgacttga taaagtcgag
     4201 gcggaggtac aaattgacag gttaattaca ggcagacttc aaagccttca aacctatgta
     4261 acacaacaac taatcagggc tgctgaaatc agggcttctg ctaatcttgc tgctactaaa
     4321 atgtctgagt gtgttcttgg acaatcaaaa agagttgact tttgtggaaa gggctaccac
     4381 cttatgtcct tcccacaagc agccccgcat ggtgttgtct tcctacatgt cacgtatgtg
     4441 ccatcccagg agaggaactt caccacagcg ccagcaattt gtcatgaagg caaagcatac
     4501 ttccctcgtg aaggtgtttt tgtgtttaat ggcacttctt ggtttattac acagaggaac
     4561 ttcttttctc cacaaataat tactacagac aatacatttg tctcaggaaa ttgtgatgtc
     4621 gttattggca tcattaacaa cacagtttat gatcctctgc aacctgagct tgactcattc
     4681 aaagaagagc tggacaagta cttcaaaaat catacatcac cagatgttga tcttggcgac
     4741 atttcaggca ttaacgcttc tgtcgtcaac attcaaaaag aaattgaccg cctcaatgag
     4801 gtcgctaaaa atttaaatga atcactcatt gaccttcaag aattgggaaa atatgagcaa
     4861 tatattaaat ggccttggta tgtttggctc ggcttcattg ctggactaat tgccatcgtc
     4921 atggttacaa tcttgctttg ttgcatgact agttgttgca gttgcctcaa gggtgcatgc
     4981 tcttgtggtt cttgctgcaa gtttgatgag gatgactctg agccagttct caagggtgtc
     5041 aaattacatt acacacttaa ttaagacgcg atcgcggcct catgggccca gctttcttgt
     5101 acaaagttgg cattataaaa aataattgct catcaatttg ttgcaacgaa caggtcacta
     5161 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     5221 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     5281 ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct
     5341 cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     5401 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     5461 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     5521 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     5581 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     5641 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat
     5701 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     5761 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     5821 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     5881 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     5941 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     6001 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     6061 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     6121 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     6181 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     6241 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     6301 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     6361 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     6421 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     6481 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     6541 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6601 aaaaaccacc g
//
