LOCUS       Exported                3767 bp ds-DNA     circular SYN 24-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Mayra Sauer
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Feb 24, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      complement(864..891)
                     /label=rrnB T2
     terminator      complement(1023..1066)
                     /label=rrnB T1
     primer_bind     1132..1149
                     /label=M13-fwd
     primer_bind     1152..1172
                     /label=Seq-pENTR-SfiI_fwd
     misc_recomb     1166..1261
                     /label=AttL1
     misc_recomb     1241..1261
                     /label=attB1
     misc_feature    1241..1261
                     /label=Gateway recomb1
     misc_feature    1265..1272
                     /label=Sfi-A
     misc_feature    2240..2244
                     /label=Sfi-B
     misc_recomb     complement(2245..2265)
                     /label=attB2
     misc_feature    complement(2245..2265)
                     /label=Gateway Recomb2
     primer_bind     complement(2338..2358)
                     /label=Seq_pENTR-SfiI_rev
     primer_bind     complement(2358..2377)
                     /label=T7
     primer_bind     complement(2378..2398)
                     /label=M13-rev
     primer_bind     complement(2407..2426)
                     /label=P4-p1R_blunt rev
     CDS             2610..3620
                     /label=aadA
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tccagtggtt ttagaaaaat ggcattccca
     1321 tctggtaaag ttgagggttg tatggtacaa gtaacttgtg gtacaactac acttaacggt
     1381 ctttggcttg atgacgtagt ttactgtcca agacatgtga tctgcacctc tgaagacatg
     1441 cttaacccta attatgaaga tttactcatt cgtaagtcta atcataattt cttggtacag
     1501 gctggtaatg ttcaactcag ggttattgga cattctatgc aaaattgtgt acttaagctt
     1561 aaggttgata cagccaatcc taagacacct aagtataagt ttgttcgcat tcaaccagga
     1621 cagacttttt cagtgttagc ttgttacaat ggttcaccat ctggtgttta ccaatgtgct
     1681 atgaggccca atttcactat taagggttca ttccttaatg gttcatgtgg tagtgttggt
     1741 tttaacatag attatgactg tgtctctttt tgttacatgc accatatgga attaccaact
     1801 ggagttcatg ctggcacaga cttagaaggt aacttttatg gaccttttgt tgacaggcaa
     1861 acagcacaag cagctggtac ggacacaact attacagtta atgttttagc ttggttgtac
     1921 gctgctgtta taaatggaga caggtggttt ctcaatcgat ttaccacaac tcttaatgac
     1981 tttaaccttg tggctatgaa gtacaattat gaacctctaa cacaagacca tgttgacata
     2041 ctaggacctc tttctgctca aactggaatt gccgttttag atatgtgtgc ttcattaaaa
     2101 gaattactgc aaaatggtat gaatggacgt accatattgg gtagtgcttt attagaagat
     2161 gaatttacac cttttgatgt tgttagacaa tgctcaggtg ttactttcca acttaattaa
     2221 gacgcgatcg cggcctcatg ggcccagctt tcttgtacaa agttggcatt ataaaaaata
     2281 attgctcatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     2341 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     2401 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     2461 catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgctg cccaaggttg
     2521 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     2581 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     2641 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     2701 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     2761 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     2821 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     2881 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     2941 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     3001 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     3061 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     3121 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     3181 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     3241 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     3301 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     3361 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     3421 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     3481 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     3541 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     3601 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
     3661 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3721 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccg
//
