LOCUS       Exported                3719 bp ds-DNA     circular SYN 24-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Mayra Sauer
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Feb 24, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      complement(864..891)
                     /label=rrnB T2
     terminator      complement(1023..1066)
                     /label=rrnB T1
     primer_bind     1132..1149
                     /label=M13-fwd
     primer_bind     1152..1172
                     /label=Seq-pENTR-SfiI_fwd
     misc_recomb     1166..1261
                     /label=AttL1
     misc_recomb     1241..1261
                     /label=attB1
     misc_feature    1241..1261
                     /label=Gateway recomb1
     misc_feature    1265..1272
                     /label=Sfi-A
     misc_feature    2192..2196
                     /label=Sfi-B
     misc_recomb     complement(2197..2217)
                     /label=attB2
     misc_feature    complement(2197..2217)
                     /label=Gateway Recomb2
     primer_bind     complement(2290..2310)
                     /label=Seq_pENTR-SfiI_rev
     primer_bind     complement(2310..2329)
                     /label=T7
     primer_bind     complement(2330..2350)
                     /label=M13-rev
     primer_bind     complement(2359..2378)
                     /label=P4-p1R_blunt rev
     CDS             2562..3572
                     /label=aadA
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tccagtgcag tgaaaagaac aatcaagggt
     1321 acacaccact ggttgttact cacaattttg acttcacttt tagttttagt ccagagtact
     1381 caatggtctt tgttcttttt tttgtatgaa aatgcctttt taccttttgc tatgggtatt
     1441 attgctatgt ctgcttttgc aatgatgttt gtcaaacata agcatgcatt tctctgtttg
     1501 tttttgttac cttctcttgc cactgtagct tattttaata tggtctatat gcctgctagt
     1561 tgggtgatgc gtattatgac atggttggat atggttgata ctagtttgtc tggttttaag
     1621 ctaaaagact gtgttatgta tgcatcagct gtagtgttac taatccttat gacagcaaga
     1681 actgtgtatg atgatggtgc taggagagtg tggacactta tgaatgtctt gacactcgtt
     1741 tataaagttt attatggtaa tgctttagat caagccattt ccatgtgggc tcttataatc
     1801 tctgttactt ctaactactc aggtgtagtt acaactgtca tgtttttggc cagaggtatt
     1861 gtttttatgt gtgttgagta ttgccctatt ttcttcataa ctggtaatac acttcagtgt
     1921 ataatgctag tttattgttt cttaggctat ttttgtactt gttactttgg cctcttttgt
     1981 ttactcaacc gctactttag actgactctt ggtgtttatg attacttagt ttctacacag
     2041 gagtttagat atatgaattc acagggacta ctcccaccca agaatagcat agatgccttc
     2101 aaactcaaca ttaaattgtt gggtgttggt ggcaaacctt gtatcaaagt agccactgta
     2161 cagcttaatt aagacgcgat cgcggcctca tgggcccagc tttcttgtac aaagttggca
     2221 ttataaaaaa taattgctca tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     2281 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
     2341 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     2401 aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     2461 tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     2521 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     2581 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2641 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2701 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2761 ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat
     2821 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2881 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2941 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     3001 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     3061 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     3121 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     3181 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     3241 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     3301 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     3361 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     3421 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     3481 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     3541 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     3601 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3661 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccg
//
