LOCUS       Exported                3098 bp ds-DNA     circular SYN 24-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Mayra Sauer
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Feb 24, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      complement(864..891)
                     /label=rrnB T2
     terminator      complement(1023..1066)
                     /label=rrnB T1
     primer_bind     1132..1149
                     /label=M13-fwd
     primer_bind     1152..1172
                     /label=Seq-pENTR-SfiI_fwd
     misc_recomb     1166..1261
                     /label=AttL1
     misc_recomb     1241..1261
                     /label=attB1
     misc_feature    1241..1261
                     /label=Gateway recomb1
     misc_feature    1265..1272
                     /label=Sfi-A
     misc_feature    1571..1575
                     /label=Sfi-B
     misc_recomb     complement(1576..1596)
                     /label=attB2
     misc_feature    complement(1576..1596)
                     /label=Gateway Recomb2
     primer_bind     complement(1669..1689)
                     /label=Seq_pENTR-SfiI_rev
     primer_bind     complement(1689..1708)
                     /label=T7
     primer_bind     complement(1709..1729)
                     /label=M13-rev
     primer_bind     complement(1738..1757)
                     /label=P4-p1R_blunt rev
     CDS             1941..2951
                     /label=aadA
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tcctctaaaa tgtcagatgt aaagtgcaca
     1321 tcagtagtct tactctcagt tttgcaacaa ctcagagtag aatcatcatc taaattgtgg
     1381 gctcaatgtg tccagttaca caatgacatt ctcttagcta aagatactac tgaagccttt
     1441 gaaaaaatgg tttcactact ttctgttttg ctttccatgc agggtgctgt agacataaac
     1501 aagctttgtg aagaaatgct ggacaacagg gcaaccttac aacttaatta agacgcgatc
     1561 gcggcctcat gggcccagct ttcttgtaca aagttggcat tataaaaaat aattgctcat
     1621 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
     1681 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     1741 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc
     1801 tctagaccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac
     1861 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
     1921 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     1981 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     2041 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     2101 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     2161 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     2221 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     2281 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     2341 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     2401 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     2461 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     2521 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     2581 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     2641 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     2701 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     2761 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     2821 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     2881 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     2941 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt
     3001 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3061 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccg
//
