LOCUS       Exported                4430 bp ds-DNA     circular SYN 24-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Mayra Sauer
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Feb 24, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      complement(864..891)
                     /label=rrnB T2
     terminator      complement(1023..1066)
                     /label=rrnB T1
     primer_bind     1132..1149
                     /label=M13-fwd
     primer_bind     1152..1172
                     /label=Seq-pENTR-SfiI_fwd
     misc_recomb     1166..1261
                     /label=AttL1
     misc_recomb     1241..1261
                     /label=attB1
     misc_feature    1241..1261
                     /label=Gateway recomb1
     misc_feature    1265..1272
                     /label=Sfi-A
     misc_feature    2903..2907
                     /label=Sfi-B
     misc_recomb     complement(2908..2928)
                     /label=attB2
     misc_feature    complement(2908..2928)
                     /label=Gateway Recomb2
     primer_bind     complement(3001..3021)
                     /label=Seq_pENTR-SfiI_rev
     primer_bind     complement(3021..3040)
                     /label=T7
     primer_bind     complement(3041..3061)
                     /label=M13-rev
     primer_bind     complement(3070..3089)
                     /label=P4-p1R_blunt rev
     CDS             3273..4283
                     /label=aadA
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tccgctgaaa atgtaacagg actctttaaa
     1321 gattgtagta aggtaatcac tgggttacat cctacacagg cacctacaca cctcagtgtt
     1381 gacactaaat tcaaaactga aggtttatgt gttgacatac ctggcatacc taaggacatg
     1441 acctatagaa gactcatctc tatgatgggt tttaaaatga attatcaagt taatggttac
     1501 cctaacatgt ttatcacccg cgaagaagct ataagacatg tacgtgcatg gattggcttc
     1561 gatgtcgagg ggtgtcatgc tactagagaa gctgttggta ccaatttacc tttacagcta
     1621 ggtttttcta caggtgttaa cctagttgct gtacctacag gttatgttga tacacctaat
     1681 aatacagatt tttccagagt tagtgctaaa ccaccgcctg gagatcaatt taaacacctc
     1741 ataccactta tgtacaaagg acttccttgg aatgtagtgc gtataaagat tgtacaaatg
     1801 ttaagtgaca cacttaaaaa tctctctgac agagtcgtat ttgtcttatg ggcacatggc
     1861 tttgagttga catctatgaa gtattttgtg aaaataggac ctgagcgcac ctgttgtcta
     1921 tgtgatagac gtgccacatg cttttccact gcttcagaca cttatgcctg ttggcatcat
     1981 tctattggat ttgattacgt ctataatccg tttatgattg atgttcaaca atggggtttt
     2041 acaggtaacc tacaaagcaa ccatgatctg tattgtcaag tccatggtaa tgcacatgta
     2101 gctagttgtg atgcaatcat gactaggtgt ctagctgtcc acgagtgctt tgttaagcgt
     2161 gttgactgga ctattgaata tcctataatt ggtgatgaac tgaagattaa tgcggcttgt
     2221 agaaaggttc aacacatggt tgttaaagct gcattattag cagacaaatt cccagttctt
     2281 cacgacattg gtaaccctaa agctattaag tgtgtacctc aagctgatgt agaatggaag
     2341 ttctatgatg cacagccttg tagtgacaaa gcttataaaa tagaagaatt attctattct
     2401 tatgccacac attctgacaa attcacagat ggtgtatgcc tattttggaa ttgcaatgtc
     2461 gatagatatc ctgctaattc cattgtttgt agatttgaca ctagagtgct atctaacctt
     2521 aacttgcctg gttgtgatgg tggcagtttg tatgtaaata aacatgcatt ccacacacca
     2581 gcttttgata aaagtgcttt tgttaattta aaacaattac catttttcta ttactctgac
     2641 agtccatgtg agtctcatgg aaaacaagta gtgtcagata tagattatgt accactaaag
     2701 tctgctacgt gtataacacg ttgcaattta ggtggtgctg tctgtagaca tcatgctaat
     2761 gagtacagat tgtatctcga tgcttataac atgatgatct cagctggctt tagcttgtgg
     2821 gtttacaaac aatttgatac ttataacctc tggaacactt ttacaagact tcagcttaat
     2881 taagacgcga tcgcggcctc atgggcccag ctttcttgta caaagttggc attataaaaa
     2941 ataattgctc atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     3001 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     3061 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     3121 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg
     3181 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     3241 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     3301 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     3361 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     3421 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     3481 agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     3541 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     3601 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     3661 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     3721 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     3781 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     3841 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     3901 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     3961 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     4021 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     4081 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     4141 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     4201 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     4261 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     4321 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4381 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
//
