LOCUS       Exported                3671 bp ds-DNA     circular SYN 24-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Mayra Sauer
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Feb 24, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      complement(864..891)
                     /label=rrnB T2
     terminator      complement(1023..1066)
                     /label=rrnB T1
     primer_bind     1132..1149
                     /label=M13-fwd
     primer_bind     1152..1172
                     /label=Seq-pENTR-SfiI_fwd
     misc_recomb     1166..1261
                     /label=AttL1
     misc_recomb     1241..1261
                     /label=attB1
     misc_feature    1241..1261
                     /label=Gateway recomb1
     misc_feature    1265..1272
                     /label=Sfi-A
     misc_feature    2144..2148
                     /label=Sfi-B
     misc_recomb     complement(2149..2169)
                     /label=attB2
     misc_feature    complement(2149..2169)
                     /label=Gateway Recomb2
     primer_bind     complement(2242..2262)
                     /label=Seq_pENTR-SfiI_rev
     primer_bind     complement(2262..2281)
                     /label=T7
     primer_bind     complement(2282..2302)
                     /label=M13-rev
     primer_bind     complement(2311..2330)
                     /label=P4-p1R_blunt rev
     CDS             2514..3524
                     /label=aadA
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tccgatttgt ttatgagaat cttcacaatt
     1321 ggaactgtaa ctttgaagca aggtgaaatc aaggatgcta ctccttcaga ttttgttcgc
     1381 gctactgcaa cgataccgat acaagcctca ctccctttcg gatggcttat tgttggcgtt
     1441 gcacttcttg ctgtttttca gagcgcttcc aaaatcataa ccctcaaaaa gagatggcaa
     1501 ctagcactct ccaagggtgt tcactttgtt tgcaacttgc tgttgttgtt tgtaacagtt
     1561 tactcacacc ttttgctcgt tgctgctggc cttgaagccc cttttctcta tctttatgct
     1621 ttagtctact tcttgcagag tataaacttt gtaagaataa taatgaggct ttggctttgc
     1681 tggaaatgcc gttccaaaaa cccattactt tatgatgcca actattttct ttgctggcat
     1741 actaattgtt acgactattg tataccttac aatagtgtaa cttcttcaat tgtcattact
     1801 tcaggtgatg gcacaacaag tcctatttct gaacatgact accagattgg tggttatact
     1861 gaaaaatggg aatctggagt aaaagactgt gttgtattac acagttactt cacttcagac
     1921 tattaccagc tgtactcaac tcaattgagt acagacactg gtgttgaaca tgttaccttc
     1981 ttcatctaca ataaaattgt tgatgagcct gaagaacatg tccaaattca cacaatcgac
     2041 ggttcatccg gagttgttaa tccagtaatg gaaccaattt atgatgaacc gacgacgact
     2101 actagcgtgc ctttgcttaa ttaagacgcg atcgcggcct catgggccca gctttcttgt
     2161 acaaagttgg cattataaaa aataattgct catcaatttg ttgcaacgaa caggtcacta
     2221 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     2281 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     2341 ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct
     2401 cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     2461 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     2521 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     2581 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     2641 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     2701 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat
     2761 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     2821 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     2881 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     2941 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     3001 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     3061 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     3121 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     3181 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     3241 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     3301 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     3361 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     3421 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     3481 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     3541 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     3601 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3661 aaaaaccacc g
//
