LOCUS       Exported                4103 bp ds-DNA     circular SYN 24-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Mayra Sauer
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Feb 24, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      complement(864..891)
                     /label=rrnB T2
     terminator      complement(1023..1066)
                     /label=rrnB T1
     primer_bind     1132..1149
                     /label=M13-fwd
     primer_bind     1152..1172
                     /label=Seq-pENTR-SfiI_fwd
     misc_recomb     1166..1261
                     /label=AttL1
     misc_recomb     1241..1261
                     /label=attB1
     misc_feature    1241..1261
                     /label=Gateway recomb1
     misc_feature    1265..1272
                     /label=Sfi-A
     misc_feature    2576..2580
                     /label=Sfi-B
     misc_recomb     complement(2581..2601)
                     /label=attB2
     misc_feature    complement(2581..2601)
                     /label=Gateway Recomb2
     primer_bind     complement(2674..2694)
                     /label=Seq_pENTR-SfiI_rev
     primer_bind     complement(2694..2713)
                     /label=T7
     primer_bind     complement(2714..2734)
                     /label=M13-rev
     primer_bind     complement(2743..2762)
                     /label=P4-p1R_blunt rev
     CDS             2946..3956
                     /label=aadA
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tcctctgata atggacccca aaatcagcga
     1321 aatgcacccc gcattacgtt tggtggaccc tcagattcaa ctggcagtaa ccagaatgga
     1381 gaacgcagtg gggcgcgatc aaaacaacgt cggccccaag gtttacccaa taatactgcg
     1441 tcttggttca ccgctctcac tcaacatggc aaggaagacc ttaaattccc tcgaggacaa
     1501 ggcgttccaa ttaacaccaa tagcagtcca gatgaccaaa ttggctacta ccgaagagct
     1561 accagacgaa ttcgtggtgg tgacggtaaa atgaaagatc tcagtccaag atggtatttc
     1621 tactacctag gaactgggcc agaagctgga cttccctatg gtgctaacaa agacggcatc
     1681 atatgggttg caactgaggg agccttgaat acaccaaaag atcacattgg cacccgcaat
     1741 cctgctaaca atgctgcaat cgtgctacaa cttcctcaag gaacaacatt gccaaaaggc
     1801 ttctacgcag aagggagcag aggcggcagt caagcctctt ctcgttcctc atcacgtagt
     1861 cgcaacagtt caagaaattc aactccaggc agcagtaggg gaacttctcc tgctagaatg
     1921 gctggcaatg gcggtgatgc tgctcttgct ttgctgctgc ttgacagatt gaaccagctt
     1981 gagagcaaaa tgtctggtaa aggccaacaa caacaaggcc aaactgtcac taagaaatct
     2041 gctgctgagg cttctaagaa gcctcggcaa aaacgtactg ccactaaagc atacaatgta
     2101 acacaagctt tcggcagacg tggtccagaa caaacccaag gaaattttgg ggaccaggaa
     2161 ctaatcagac aaggaactga ttacaaacat tggccgcaaa ttgcacaatt tgcccccagc
     2221 gcttcagcgt tcttcggaat gtcgcgcatt ggcatggaag tcacaccttc gggaacgtgg
     2281 ttgacctaca caggtgccat caaattggat gacaaagatc caaatttcaa agatcaagtc
     2341 attttgctga ataagcatat tgacgcatac aaaacattcc caccaacaga gcctaaaaag
     2401 gacaaaaaga agaaggctga tgaaactcaa gccttaccgc agagacagaa gaaacagcaa
     2461 actgtgactc ttcttcctgc tgcagatttg gatgatttct ccaaacaatt gcaacaatcc
     2521 atgagcagtg ctgactcaac tcaggccctt aattaagacg cgatcgcggc ctcatgggcc
     2581 cagctttctt gtacaaagtt ggcattataa aaaataattg ctcatcaatt tgttgcaacg
     2641 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     2701 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     2761 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     2821 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     2881 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     2941 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     3001 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     3061 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     3121 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga
     3181 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     3241 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     3301 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     3361 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     3421 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     3481 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     3541 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     3601 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     3661 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     3721 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     3781 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     3841 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     3901 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     3961 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     4021 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4081 gcttgcaaac aaaaaaacca ccg
//
