LOCUS       Exported                4763 bp ds-DNA     circular SYN 24-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Mayra Sauer
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Feb 24, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      complement(864..891)
                     /label=rrnB T2
     terminator      complement(1023..1066)
                     /label=rrnB T1
     primer_bind     1132..1149
                     /label=M13-fwd
     primer_bind     1152..1172
                     /label=Seq-pENTR-SfiI_fwd
     misc_recomb     1166..1261
                     /label=AttL1
     misc_recomb     1241..1261
                     /label=attB1
     misc_feature    1241..1261
                     /label=Gateway recomb1
     misc_feature    1265..1272
                     /label=Sfi-A
     misc_feature    3236..3240
                     /label=Sfi-B
     misc_recomb     complement(3241..3261)
                     /label=attB2
     misc_feature    complement(3241..3261)
                     /label=Gateway Recomb2
     primer_bind     complement(3334..3354)
                     /label=Seq_pENTR-SfiI_rev
     primer_bind     complement(3354..3373)
                     /label=T7
     primer_bind     complement(3374..3394)
                     /label=M13-rev
     primer_bind     complement(3403..3422)
                     /label=P4-p1R_blunt rev
     CDS             3606..4616
                     /label=aadA
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tccgcataca ctcgctatgt cgataacaac
     1321 ttctgtggcc ctgatggcta ccctcttgag tgcattaaag accttctagc acgtgctggt
     1381 aaagcttcat gcactttgtc cgaacaactg gactttattg acactaagag gggtgtatac
     1441 tgctgccgtg aacatgagca tgaaattgct tggtacacgg aacgttctga aaagagctat
     1501 gaattgcaga caccttttga aattaaattg gcaaagaaat ttgacacctt caatggggaa
     1561 tgtccaaatt ttgtatttcc cttaaattcc ataatcaaga ctattcaacc aagggttgaa
     1621 aagaaaaagc ttgatggctt tatgggtaga attcgatctg tctatccagt tgcgtcacca
     1681 aatgaatgca accaaatgtg cctttcaact ctcatgaagt gtgatcattg tggtgaaact
     1741 tcatggcaga cgggcgattt tgttaaagcc acttgcgaat tttgtggcac tgagaatttg
     1801 actaaagaag gtgccactac ttgtggttac ttaccccaaa atgctgttgt taaaatttat
     1861 tgtccagcat gtcacaattc agaagtagga cctgagcata gtcttgccga ataccataat
     1921 gaatctggct tgaaaaccat tcttcgtaag ggtggtcgca ctattgcctt tggaggctgt
     1981 gtgttctctt atgttggttg ccataacaag tgtgcctatt gggttccacg tgctagcgct
     2041 aacataggtt gtaaccatac aggtgttgtt ggagaaggtt ccgaaggtct taatgacaac
     2101 cttcttgaaa tactccaaaa agagaaagtc aacatcaata ttgttggtga ctttaaactt
     2161 aatgaagaga tcgccattat tttggcatct ttttctgctt ccacaagtgc ttttgtggaa
     2221 actgtgaaag gtttggatta taaagcattc aaacaaattg ttgaatcctg tggtaatttt
     2281 aaagttacaa aaggaaaagc taaaaaaggt gcctggaata ttggtgaaca gaaatcaata
     2341 ctgagtcctc tttatgcatt tgcatcagag gctgctcgtg ttgtacgatc aattttctcc
     2401 cgcactcttg aaactgctca aaattctgtg cgtgttttac agaaggccgc tataacaata
     2461 ctagatggaa tttcacagta ttcactgaga ctcattgatg ctatgatgtt cacatctgat
     2521 ttggctacta acaatctagt tgtaatggcc tacattacag gtggtgttgt tcagttgact
     2581 tcgcagtggc taactaacat ctttggcact gtttatgaaa aactcaaacc cgtccttgat
     2641 tggcttgaag agaagtttaa ggaaggtgta gagtttctta gagacggttg ggaaattgtt
     2701 aaatttatct caacctgtgc ttgtgaaatt gtcggtggac aaattgtcac ctgtgcaaag
     2761 gaaattaagg agagtgttca gacattcttt aagcttgtaa ataaattttt ggctttgtgt
     2821 gctgactcta tcattattgg tggagctaaa cttaaagcct tgaatttagg tgaaacattt
     2881 gtcacgcact caaagggatt gtacagaaag tgtgttaaat ccagagaaga aactggccta
     2941 ctcatgcctc taaaagcccc aaaagaaatt atcttcttag agggagaaac acttcccaca
     3001 gaagtgttaa cagaggaagt tgtcttgaaa actggtgatt tacaaccatt agaacaacct
     3061 actagtgaag ctgttgaagc tccattggtt ggtacaccag tttgtattaa cgggcttatg
     3121 ttgctcgaaa tcaaagacac agaaaagtac tgtgcccttg cacctaatat gatggtaaca
     3181 aacaatacct tcacactcaa aggcggtctt aattaagacg cgatcgcggc ctcatgggcc
     3241 cagctttctt gtacaaagtt ggcattataa aaaataattg ctcatcaatt tgttgcaacg
     3301 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     3361 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     3421 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     3481 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     3541 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     3601 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     3661 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     3721 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     3781 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga
     3841 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     3901 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     3961 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     4021 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     4081 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     4141 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     4201 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     4261 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     4321 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     4381 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     4441 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     4501 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     4561 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     4621 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     4681 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4741 gcttgcaaac aaaaaaacca ccg
//
