LOCUS       pEntry_HCoV-NL63        4280 bp ds-DNA     circular SYN 22-FEB-2021
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Mayra Sauer
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 22, 2021 from SnapGene 5.2.4 to Vector NTI(R) 
            format
            https://www.snapgene.com
COMMENT     VNTDATE|941932800|
COMMENT     VNTDBDATE|941932800|
COMMENT     VNTNAME|pEntry_HCoV-NL63-nsp4|
COMMENT     VNTAUTHORNAME|Mayra Sauer|
FEATURES             Location/Qualifiers
     regulatory      2353..2362
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tccggtttta aacgtactta taattttctg
     1321 tggtatgtat gtttatttgt tgttgcattg tttattggtg tctcatttat tgattataca
     1381 accactgtaa ctagctttca tggttatgat tttaagtaca ttgagaatgg tcagttgaag
     1441 gtgtttgaag cacctttaca ctgtgttcgt aatgtttttg ataattttaa tcaatggcat
     1501 gaggctaagt ttggtgttgt tactactaat agtgataaat gtcctatagt tgttggtgtt
     1561 tcagagcgta ttaatgttgt tcctggtgtt ccaacaaatg tatatttggt aggaaagact
     1621 cttgttttta cattacaggc tgcttttgga aacacaggtg tttgttatga ctttgatggt
     1681 gttaccacta gtgataagtg tatttttaat tctgcttgta ctaggttgga aggtttgggt
     1741 ggtgacaatg tttattgtta caacactgat cttattgaag gttctaaacc ttatagtact
     1801 ttacagccca atgcgtatta taagtatgat gctaaaaatt atgtacgttt tccagaaatt
     1861 ttagctagag gttttggctt acgtactatt agaactttgg ctacacgtta ttgtagagtt
     1921 ggtgaatgcc gtgactcaca taaaggtgtt tgttttggtt ttgataaatg gtatgttaat
     1981 gatggacgtg ttgatgacgg ttacatttgt ggtgatggtc ttatagacct tcttgttaat
     2041 gtactctcaa tctttagttc atcttttagc gttgtggcta tgtctggaca tatgttgttt
     2101 aattttcttt ttgcagcatt tattacattt ttgtgctttt tagttactaa atttaaacgt
     2161 gtttttggtg atctttctta tggtgttttt actgttgttt gtgcaacttt gattaataac
     2221 atttcttatg ttgttactca aaatttattt tttatgttgc tttatgctat tttgtatttt
     2281 gtttttacta ggacagtgcg ttatgcttgg atttggcata ttgcatacat tgttgcatac
     2341 ttcttgttaa taccatggtg gcttctcaca tggtttagtt ttgctgcatt tttagagctt
     2401 ttacctaatg tttttaagtt aaaaatctct actcaattgt ttgaaggtga taagtttata
     2461 ggtacttttg agagtgctgc tgcaggtaca tttgttcttg acatgcgttc ttatgaaagg
     2521 ctgataaata ctatttcacc tgagaaactt aagaattatg ctgcaagtta taataaatat
     2581 aaatattata gtggtagtgc tagtgaggct gattatcgtt gtgcttgtta tgctcattta
     2641 gccaaggcta tgttagatta tgcaaaagat cataatgaca tgttatattc tccacctact
     2701 attagctaca attccacctt acaacttaat taagacgcga tcgcggcctc atgggcccag
     2761 ctttcttgta caaagttggc attataaaaa ataattgctc atcaatttgt tgcaacgaac
     2821 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     2881 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     2941 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     3001 gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga
     3061 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     3121 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     3181 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     3241 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     3301 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg
     3361 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     3421 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     3481 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     3541 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     3601 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     3661 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     3721 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     3781 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     3841 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     3901 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     3961 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     4021 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     4081 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     4141 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     4201 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4261 tgcaaacaaa aaaaccaccg
//
