LOCUS       pYFAC-CH2              15498 bp    DNA     circular UNA 02-JUL-2021
DEFINITION  Gibson Assembly of 4 gene expression cassette into Digestion of
            pKW-CA-URA3 (final design) Copy.
ACCESSION   urn.local...qq-dk7ubyn
VERSION     urn.local...qq-dk7ubyn
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    4..1003
                     /standard_name="PyrG(A.fumigatus)_1kb_upstream"
     misc_feature    complement(132..150)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="pKW-pyrG-R"
     misc_feature    1004..1908
                     /standard_name="Afu2g08360(PyrG)_ORF"
     misc_feature    1909..2908
                     /standard_name="PyrG(A.fumigatus)_1kb_downstream"
     misc_feature    2942..3802
                     /standard_name="AmpR"
     misc_feature    3897..4579
                     /standard_name="ColE1 ori"
     misc_feature    4618..6293
                     /note="Geneious type: Inserted sequence"
                     /standard_name="pBF-CA-ADH2-URA3@revised extraction
                     (reversed) 2"
     primer_bind     4618..4640
                     /standard_name="6 F"
     primer_bind     4618..4640
                     /standard_name="pBF-CA-ADH2-URA3@revised extraction
                     (reversed) 2 (fwd) - Digestion of pKW20088_pKW1903
                     (modified) - Fragment 3"
     rep_origin      4631..5122
     primer_bind     5111..5141
                     /standard_name="overhang"
     misc_feature    complement(5145..5178)
                     /standard_name="loxP"
     misc_feature    complement(5179..6292)
                     /note="Geneious type: misc. marker"
                     /standard_name="URA3"
     primer_bind     5179..5207
                     /standard_name="URA3-R for loxP removal"
     misc_feature    complement(6273..6293)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="1,681 R"
     misc_feature    complement(6273..6293)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="pBF-CA-ADH2-URA3@revised extraction
                     (reversed) 2 (rev) - Digestion of pKW20088_pKW1903
                     (modified) - Fragment 3"
     misc_feature    6293
                     /standard_name="loxP"
     misc_feature    6493..9109
                     /standard_name="AMA1 ori for Aspergillus(1)"
     misc_feature    9110..11386
                     /standard_name="AMA1 ori for Aspergillus"
     misc_feature    11387..11740
                     /standard_name="AMA1 ori for Aspergillus(2)"
     promoter        <12486..>12878
                     /standard_name="alcA Promoter"
     misc_feature    12486..15498
                     /note="Geneious type: Inserted sequence"
                     /standard_name="4 gene expression cassette"
     misc_feature    12486..>12882
                     /note="Geneious type: Inserted sequence"
                     /standard_name="DQ076245 AlcA - alcA Promoter (oakley)
                     PacI 5'trunc"
     primer_bind     12486..12507
                     /standard_name="DQ076245 AlcA - alcA Promoter (oakley)
                     PacI 5'trunc (fwd) - Digestion of pKW-CA-URA3 (final
                     design)"
     primer_bind     12486..12507
                     /standard_name="4 gene expression cassette (fwd) -
                     Digestion of pKW-CA-URA3 (final design) Copy"
     protein_bind    12515..12531
                     /gene="alcA"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="activates transcription of alcA"
                     /bound_moiety="AlcR transcriptional activator"
                     /standard_name="alcA protein bind"
     protein_bind    12565..12576
                     /gene="alcA"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="activates transcription of alcA"
                     /bound_moiety="AlcR transcriptional activator"
                     /standard_name="alcA protein bind"
     protein_bind    12582..12587
                     /gene="alcA"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="represses transcription of alcA"
                     /bound_moiety="CreA transcriptional repressor"
                     /standard_name="alcA protein bind"
     protein_bind    12681..12719
                     /gene="alcA"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="activates transcription of alcA"
                     /bound_moiety="AlcR transcriptional activator"
                     /standard_name="alcA protein bind"
     protein_bind    12716..12760
                     /gene="alcA"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="represses transcription of alcA"
                     /bound_moiety="CreA transcriptional repressor"
                     /standard_name="alcA protein bind"
     primer_bind     12797..12819
                     /standard_name="alcA-seq-F"
     primer_bind     12847..12906
                     /standard_name="PalcA-T1-F"
     misc_feature    complement(12859..12878)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="AlcA-R"
     misc_feature    12879..>12882
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CA"
     misc_feature    12882
                     /standard_name="can be removed in recombination cloning"
     misc_feature    12883..15498
                     /note="Geneious type: Inserted sequence"
                     /standard_name="bi-ter1+PalcA - Digestion of pKW-2
                     micron-alcSM(NotI/SgfI) - Fragment 2 - bi-ter2+PaldA"
     misc_feature    12885
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     gene            <12886..12899
                     /locus_tag="ANIA_08984"
                     /old_locus_tag="AN8984.4"
                     /standard_name="gene"
     CDS             <12886..12899
                     /locus_tag="ANIA_08984"
                     /old_locus_tag="AN8984.4"
                     /note="transcript_id=CADANIAT00007871"
                     /codon_start=1
                     /product="conserved hypothetical protein"
                     /protein_id="CBF84517.1"
                     /db_xref="GI:259486566"
                     /db_xref="EnsemblGenomes-Gn:CADANIAG00007871"
                     /db_xref="EnsemblGenomes-Tr:CADANIAT00007871"
                     /db_xref="GOA:Q5ARU6"
                     /db_xref="UniProtKB/TrEMBL:Q5ARU6"
                     /translation="MAGDERVSITAVSWTLGTLSILLVALRLYTRLLITRCRGWDDLF
                     VTIALVNAIICSALTQIAVSHGLGKHEADIPNPNDRISAAKYATIAPSFSVISTVTGK
                     ISVVIFLLRLLTLSAKPWHRWFLHSLTLASIAWNILGIVAIIGYCRPTRKIWLPETEG
                     SCMSSRFQLVAGISQSAFNAFADWALALFPAILFRKVQLPLLKKVVVIAVLGSGVLDM
                     ARHHEMVYVELRQQPKIWNEQMYLIIICASVPTMPQSLNALLHLCKTYHKYNSSSSRC
                     NLSRPSIREPGVLLKRMQDSSLFDAPAEHAGSQENILACNEGELNTNIGCMIDLTVAQ
                     DSDIGDIVLVQGGTN"
                     /standard_name="conserved hypothetical protein CDS"
     misc_feature    12886..>13235
                     /note="Geneious type: Inserted sequence"
                     /standard_name="bi-terminator1 - PalcM/S  - bi-terminator2
                     - PaldA (reversed)"
     primer_bind     12886..12906
                     /standard_name="bi-terminator1-F"
     terminator      12900..13217
                     /standard_name="Terminator"
     misc_feature    complement(13216..>13241)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="alcSM-T1-R"
     misc_feature    complement(13216..13235)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="bi-terminator1-R"
     gene            complement(13218..>13235)
                     /locus_tag="ANIA_08983"
                     /old_locus_tag="AN8983.4"
                     /standard_name="gene"
     CDS             complement(13218..>13235)
                     /locus_tag="ANIA_08983"
                     /old_locus_tag="AN8983.4"
                     /note="transcript_id=CADANIAT00007872"
                     /codon_start=1
                     /product="MFS transporter, putative (AFU_orthologue;
                     AFUA_2G02040)"
                     /protein_id="CBF84519.1"
                     /db_xref="GI:259486567"
                     /db_xref="EnsemblGenomes-Gn:CADANIAG00007872"
                     /db_xref="EnsemblGenomes-Tr:CADANIAT00007872"
                     /db_xref="GOA:Q5ARU7"
                     /db_xref="InterPro:IPR011701"
                     /db_xref="InterPro:IPR016196"
                     /db_xref="UniProtKB/TrEMBL:Q5ARU7"
                     /translation="MDDISQSEVPGTIFIVGTDATKLGEADVTTSNDIVLVPRPLETP
                     RDPLNWPKSKKLWTLFLATLFATVVAYGENNLGAPWTEVAEDIDVTMKDMNGGSALNY
                     LLLGFVNVLWIPTAMKLGRKVVYIFSMTIIMVSSVWNAYIYGVAQWYLNCLVGGIGQS
                     AYQALIQLTVFDIFFAHQRGTMLAVYVWGQQLGSILALILGGYIAEGPGWRWSCKIVA
                     ILSVRLISVILRAAVADALQGSVVLLFIFTMDDSLFPRHALAIHQTTTVPQTQDSHQP
                     EAENDTKKEQSVNLNRQPTISQGRADLLPRNYLHVLAPIHRWKEDKTTWWQYFRRPFH
                     LFAFPNIIMAGLIYAFANTAGIVSFNTISEIMTGAPYNWSTGPTGLMFLAALVGSFIG
                     MATGALGDILTLRLARANGGYKEPEMRLWTLSLSFVYGAVGYFMYGWSASDGKSWVLI
                     AVGLGGMIAQQVSAASVATAYAMECFEGISSELVVVLAICSSCVNFAISYSVQPFIEA
                     TDYGRTFTCFGALVLCSLAMAGAGVKWGKSWRRRCASRYWKFLDHLDYALNTA"
                     /standard_name="MFS transporter, putative (AFU orthologue;
                     AFUA 2G02040) CDS"
     misc_feature    <13242..>13991
                     /note="Geneious type: Inserted sequence"
                     /standard_name="CM001205_[HPS1_cluster]
                     extraction-reductase (reversed) - BN001307 extraction -
                     CM001205_[HPS1_cluster] extraction-transporter"
     primer_bind     13242..13261
                     /standard_name="BN001307 extraction (fwd) -
                     CM001205_[HPS1_cluster] extraction-reductase (reversed)"
     primer_bind     13242..13261
                     /standard_name="Pro-F"
     promoter        13318..13700
                     /standard_name="Bidirectional promoter PalcS/M"
     misc_feature    complement(13968..13991)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="Pro-R"
     primer_bind     <13992..14026
                     /standard_name="alcSM-T2-F"
     misc_feature    13999
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     mRNA            <14000..14016
                     /locus_tag="ANIA_08982"
                     /old_locus_tag="AN8982.4"
                     /note="transcript_id=CADANIAT00007873"
                     /standard_name="transcript id=CADANIAT00007873 mRNA"
     gene            <14000..14016
                     /locus_tag="ANIA_08982"
                     /old_locus_tag="AN8982.4"
                     /standard_name="gene"
     CDS             <14000..14016
                     /locus_tag="ANIA_08982"
                     /old_locus_tag="AN8982.4"
                     /note="transcript_id=CADANIAT00007873"
                     /codon_start=1
                     /product="hypothetical fructosyl amine:oxygen
                     oxidoreductase (Eurofung)"
                     /protein_id="CBF84521.1"
                     /db_xref="GI:259486568"
                     /db_xref="EnsemblGenomes-Gn:CADANIAG00007873"
                     /db_xref="EnsemblGenomes-Tr:CADANIAT00007873"
                     /db_xref="GOA:Q5ARU8"
                     /db_xref="InterPro:IPR006076"
                     /db_xref="UniProtKB/TrEMBL:Q5ARU8"
                     /translation="MSQLTKDSSILIIGGGTWGCSTALQLARAGYKNVTVLERSPIPS
                     PSAAGNDVNKIMEEGAPSDTDTPEQYVWNRMHQITANAWKTDPVFRPFYHPTGFIMAA
                     SQDDAMPHVDKYISTCRDKLRLLNSAADFRATMPLGVLTGDFSGWKGFFREAGAGWVF
                     ARGALEAAYKEASRLGVKFVTGDEGGVKSLLYTDDQSDIVGAKTADEKEHRADRVILC
                     AGAGSDQLLDFEKQLRPTAWTLAHIQMTEEERKLWKDLPVLFNVNSGFFIEPDSTGQL
                     KFVDEHPGYCNIPTSEVLNDFPKSIPFAKHQIPLQSEHAARQFLRDTVPQIADRPFAF
                     ARICWDADTPDRQFLIDQHPKYKSLTVAVGGSGNGFMMMPAVGIAIKQRLEGVLEEKL
                     KYAFRWRPETAVHRDWRDTQDRFGGTGTVRNFQDVEVWTRVGEEII"
                     /standard_name="hypothetical fructosyl amine:oxygen
                     oxidoreductase (Eurofung) CDS"
     misc_feature    <14000..15498
                     /note="Geneious type: Inserted sequence"
                     /standard_name="bi-terminator1 - PalcM/S  - bi-terminator2
                     - PaldA (reversed)"
     primer_bind     14000..14026
                     /standard_name="bi-terminator2-F"
     terminator      14017..14874
                     /standard_name="Terminator"
     source          <14871..>14893
                     /organism="Aspergillus nidulans FGSC A4"
                     /mol_type="genomic DNA"
                     /strain="FGSC A4"
                     /db_xref="taxon:227321"
                     /chromosome="VII"
     misc_feature    complement(14871..14930)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="PaldA-T2-R"
     misc_feature    complement(14871..14893)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="bi-terminator2-R"
     misc_feature    <14871..>14893
                     /note="contig 1.168 1..229305(-1)"
                     /standard_name="contig 1.168 1..229305(-1)"
     mRNA            complement(14875..>14893)
                     /locus_tag="ANIA_08981"
                     /old_locus_tag="AN8981.4"
                     /note="transcript_id=CADANIAT00007874"
                     /standard_name="transcript id=CADANIAT00007874 mRNA"
     gene            complement(14875..>14893)
                     /locus_tag="ANIA_08981"
                     /old_locus_tag="AN8981.4"
                     /standard_name="gene"
     CDS             complement(14875..>14893)
                     /locus_tag="ANIA_08981"
                     /old_locus_tag="AN8981.4"
                     /note="transcript_id=CADANIAT00007874"
                     /codon_start=1
                     /product="AlcS [Source:UniProtKB/TrEMBL;Acc:Q460G9]"
                     /protein_id="CBF84523.1"
                     /db_xref="GI:259486569"
                     /db_xref="EnsemblGenomes-Gn:CADANIAG00007874"
                     /db_xref="EnsemblGenomes-Tr:CADANIAT00007874"
                     /db_xref="GOA:Q460G9"
                     /db_xref="InterPro:IPR000791"
                     /translation="MTTEISNGEAKGHHLSTIPSSITLSAEQFEKLYLSPMMRQQPSL
                     ARKVGNPTPLALGGFVITTTPLSCCLMAWRGSSGNGIAFIGPIIFLGGLLLLITSILE
                     FILGNTFPCVVFGTIGGFWFAFAATMIPSFNAAAPYSSSATSTTAGLTSASFMNTYAF
                     LFITMAVLMLIFLLCATRTNVVYTLIFLSLLLVFLLLSAGYWRLGEGDAAVGDRCIKG
                     AGASLFVASLLGFYLLIAQLFDAVGLPITLPVGDMERFWPRSQK"
                     /standard_name="AlcS [Source:UniProtKB/TrEMBL;Acc:Q460G9]
                     CDS"
     misc_feature    14894..14898
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     promoter        complement(14899..15498)
                     /standard_name="Promoter PaldA"
     primer_bind     14899..14918
                     /standard_name="PaldA (reversed) (fwd) - bi-terminator2"
     misc_feature    complement(15478..39)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="pyrG-PaldH-R"
     misc_feature    complement(15478..15498)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="PaldA (reversed) (rev) - Digestion of
                     pKW-CA-URA3-AlcA (final design)"
     misc_feature    complement(15478..15498)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="4 gene expression cassette (rev) -
                     Digestion of pKW-CA-URA3 (final design) Copy"
ORIGIN      
        1 gcccagaaaa ttacgggtat gtcctccact gtgttgaagt cagaaaaaca atcgataatt
       61 tgaagctcat taaaatgcca gttccgtcgt cgccgttgcc ctaggagcta ttccagtgct
      121 tagcttcatt catggatgta tcgtgactgg ccttcggaag gatgccaagg tcccctatcc
      181 gcacagctat gcgaccgttg agcaatgcaa gtcaaatgtg tgcatacctg gtctttggtt
      241 tggtacaccg ctatgtgaag attattaatg ctgaagtcat gatacaggcc aaagccgagc
      301 aatttaactg cgcgcagcgg gcccacgcta actacctcga gaatatgcct caaacaatgc
      361 tcttcaccct cttcgcgggt ctgaaatacc ctcacctggc aacagcaatt ggcgcttcat
      421 ggctgttttt ccgatctctc tacttgtacg gctatgtgta ctcgggtaag ccacaaggca
      481 agggcagatt gctgggaggt ttcttctggt tttctcaagg cgctctgtgg gctctgagtg
      541 tgtttggtgt tgccaaagac atgatctctt actgagagtt attctgtgtc tgacgaaata
      601 tgttgtgtat atatatatat gtacgttaaa agttccgtgg agttaccagt gattgaccaa
      661 tgttttatct tctacagttc tgcctgtcta ccccattcta gctgtacctg actacagagt
      721 agtttaattg tggttgaccc cacagtcgga ggcggaggaa tacagcaccg atgtggcctg
      781 tctccatcca gattggcacg caatttttac acgcggaaaa gatcgagata gagtacgact
      841 ttaaatttag tccccggcgg cttctatttt agaatatttg agatttgatt ctcaagcaat
      901 tgatttggtt gggtcaccct caattggata atatacctca ttgctcggct acttcaactc
      961 atcaatcacc gtcatacccc gcatataacc ctccattccc acgatgtcgt ccaagtcgca
     1021 attgacttac ggtgctcgag ccagcaagca ccccaatcct ctggcaaaga gactttttga
     1081 gattgccgaa gcaaagaaga caaacgttac cgtctctgct gatgtgacga caacccgaga
     1141 actcctggac ctcgctgacc gtacggaagc tgttggatcc aatacatatg ccgtctagca
     1201 atggactaat caacttttga tgatacaggt ctcggtccct acatcgccgt catcaagaca
     1261 cacatcgaca tcctcaccga tttcagcgtc gacactatca atggcctgaa tgtgctggct
     1321 caaaagcaca actttttgat cttcgaggac cgcaaattca tcgacatcgg caataccgtc
     1381 cagaagcaat accacggcgg tgctctgagg atctccgaat gggcccacat tatcaactgc
     1441 agcgttctcc ctggcgaggg catcgtcgag gctctggccc agaccgcatc tgcgcaagac
     1501 ttcccctatg gtcctgagag aggactgttg gtcctggcag agatgacctc caaaggatcg
     1561 ctggctacgg gcgagtatac caaggcatcg gttgactacg ctcgcaaata caagaacttc
     1621 gttatgggtt tcgtgtcgac gcgggccctg acggaagtgc agtcggatgt gtcttcagcc
     1681 tcggaggatg aagatttcgt ggtcttcacg acgggtgtga acctctcttc caaaggagat
     1741 aagcttggac agcaatacca gactcctgca tcggctattg gacgcggtgc cgactttatc
     1801 atcgccggtc gaggcatcta cgctgctccc gacccggttg aagctgcaca gcggtaccag
     1861 aaagaaggct gggaagctta tatggccaga gtatgcggca agtcatgatt tcctcttgga
     1921 gcaaaagtgt agtgccagta cgagtgttgt ggaggaaggc tgcatacatt gtgcctgtca
     1981 ttaaacgatg agctcgtccg tattggcccc tgtaatgcca tgttttccgc ccccaatcgt
     2041 caaggttttc cctttgttag attcctacca gtcatctagc aagtgaggta agctttgcca
     2101 gaaacgccaa ggctttatct atgtagtcga taagcaaagt ggactgatag cttaatatgg
     2161 aaggtccctc aggacaagtc gacctgtgca gaagagataa cagcttggca tcacgcatca
     2221 gtgcctcctc tcagacagaa taagtcgcca taagttatcg accgaaccta ggtagggtat
     2281 atttggtgac gacaatacct cccgacgata cctggattag acaccgtatg ccgcgaatta
     2341 acgccttccg accaagtccc ttttggtgat cgacacttta ggatcatctc ctgccagtcg
     2401 cccagactga cggtgttatg gtggcgcgct atggctttcc tagccggtca agctccgtgc
     2461 actagagagc ggagaaggag ggggagttga tagggaatga tatctaggta gagaagtact
     2521 ccaaccagag atatgcggat caatgctccg gcagttgtta gcatacccca acctcgttta
     2581 agtatcctct tcttccatcc atctttatca ttatttgtct atgtgcatcc ctagatcacg
     2641 tcaattattt cgataaatgg atacgtgcac tcgggaatgc gtgattggct cgtctcgtgt
     2701 tctcagttcc tcattccccc ccacgttaca ttgacaacgg agtattagcc tatggtcctg
     2761 ttcgataagc ttgctccatc gcctccacta cgtcaagcgg agtatcagac atgagctcta
     2821 ccccagatat cgcctgctta tcgctggttg ctttgtcatg caagtacaga tcacatcggc
     2881 aattctgata acagatggtg ccattgtccc gtaatcaatt gcccattgaa aaaggaagag
     2941 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     3001 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     3061 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     3121 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     3181 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     3241 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     3301 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3361 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     3421 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     3481 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     3541 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3601 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     3661 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     3721 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     3781 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     3841 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3901 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3961 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4021 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4081 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4141 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4201 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4261 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4321 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4381 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4441 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4501 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4561 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcatga
     4621 ttacgaattc atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata
     4681 taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa
     4741 gatgtaaaag actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc
     4801 tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat
     4861 cctgtgattt tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc
     4921 ttgtctaata aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt
     4981 attttttttt cttcattccg taactcttct accttcttta tttactttct aaaatccaaa
     5041 tacaaaacat aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag
     5101 atacgaggcg cgtgtaagtt acgaattcga gctcggtacc cgggataact tcgtataatg
     5161 tatgctatac gaagttatgg gtaataactg atataattaa attgaagctc taatttgtga
     5221 gtttagtata catgcattta cttataatac agttttttag ttttgctggc cgcatcttct
     5281 caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc
     5341 ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc
     5401 cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt
     5461 cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc
     5521 gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat attctccagt
     5581 agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt
     5641 tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc
     5701 attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact gcaatttgac
     5761 tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt acttggcgga
     5821 taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga tatccacatg
     5881 tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta attccttggt
     5941 ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag
     6001 cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag ctttcgacat
     6061 gatttatctt cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga atactgggca
     6121 atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt
     6181 ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaag gaaaccgaaa
     6241 tcaaaaaaaa gaataaaaaa aaaatgatga attgaattga aaagcgtggt gcagcctgat
     6301 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     6361 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6421 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
     6481 gccacgttcg ccagcttatt ttttgtatac tgttttgtga tagcacgaag tttttccacg
     6541 gtatcttgtt aaaaatatat atttgtggcg ggcttaccta catcaaatta ataagagact
     6601 aattataaac taaacacaca agcaagctac tttagggtaa aagtttataa atgcttttga
     6661 cgtataaacg ttgcttgtat ttattattac aattaaaggt ggatagaaaa cctagagact
     6721 agttagaaac taatctcagg tttgcgttaa actaaatcag agcccgagag gttaacagaa
     6781 cctagaaggg gactagatat ccgggtaggg aaacaaaaaa aaaaaacaag acagccacat
     6841 attagggaga ctagttagaa gctagttcca ggactaggaa aataaaagac aatgatacca
     6901 cagtctagtt gacaactaga tagattctag attgaggcca aagtctctga gatccaggtt
     6961 agttgcaact aatactagtt agtatctagt ctcctataac tctgaagcta gaataactta
     7021 ctactattat cctcaccact gttcagctgc gcaaacggag tgattgcaag gtgttcagag
     7081 actagttatt gactagtcag tgactagcaa taactaacaa ggtattaacc taccatgtct
     7141 gccatcaccc tgcacttcct cgggctcagc agccttttcc tcctcatttt catgctcatt
     7201 ttccttgttt aagactgtga ctagtcaaag actagtccag aaccacaaag gagaaatgtc
     7261 ttaccacttt cttcattgct tgtctctttt gcattatcca tgtctgcaac tagttagagt
     7321 ctagttagtg actagtccga cgaggacttg cttgtctccg gattgttgga ggaactctcc
     7381 agggcctcaa gatccacaac agagccttct agaagactgg tcaataacta gttggtcttt
     7441 gtctgagtct gacttacgag gttgcatact cgctcccttt gcctcgtcaa tcgatgagaa
     7501 aaagcgccaa aactcgcaat atggctttga accacacggt gctgagacta gttagaatct
     7561 agtcccaaac tagcttggat agcttacctt tgccctttgc gttgcgacag gtcttgcagg
     7621 gtatggttcc tttctcacca gctgatttag ctgccttgct accctcacgg cggatctgca
     7681 taaagagtgg ctagaggtta taaattagca ctgatcctag gtacggggct gaatgtaact
     7741 tgcctttcct ttctcatcgc gcggcaagac aggcttgctc aaattcctac cagtcacagg
     7801 ggtatgcacg gcgtacggac cacttgaact agtcacagat tagttagcaa ctagtctgca
     7861 ttgaatggct gtacttacgg gccctcgcca ttgtcctgat catttccagc ttcaccctcg
     7921 ttgctgcaaa gtagttagtg actagtcaag gactagttga aatgggagaa gaaactcacg
     7981 aattctcgac acccttagta ttgtggtcct tggacttggt gctgctatat attagctaat
     8041 acactagtta gactcacaga aacttacgca gctcgcttgc gcttcttggt aggagtcggg
     8101 gttgggagaa cagtgccttc aaacaagcct tcataccatg ctacttgact agtcagggac
     8161 tagtcaccaa gtaatctaga taggacttgc ctttggcctc catcagttcc ttcatagtgg
     8221 gaggtccatt gtgcaatgta aactccatgc cgtgggagtt cttgtccttc aagtgcttga
     8281 ccaatatgtt tctgttggca gagggaacct gtcaactagt taataactag tcagaaacta
     8341 gtatagcagt agactcactg tacgcttgag gcatcccttc actcggcagt agacttcata
     8401 tggatggata tcaggcacgc cattgtcgtc ctgtggacta gtcagtaact aggcttaaag
     8461 ctagtcgggt cggcttacta tcttgaaatc cggcagcgta agctccccgt ccttaactgc
     8521 ctcgagatag tgacagtact ctggggactt tcggagatcg ttatcgcgaa tgctcggcat
     8581 actaatcgtt gactagtctt ggactagtcc cgagcaaaaa ggattggagg aggaggagga
     8641 aggtgagagt gagacaaaga gcgaaataag agcttcaaag gctatctcta agcagtatga
     8701 aggttaagta tctagttctt gactagattt aaaagagatt tcgactagtt atgtacctgg
     8761 agtttggata taggaatgtg ttgtggtaac gaaatgtaag ggggaggaaa gaaaaagtcg
     8821 gtcaagaggt aactctaagt cggccattcc tttttgggag gcgctaacca taaacggcat
     8881 ggtcgactta gagttagctc agggaattta gggagttatc tgcgaccact ggactctcag
     8941 gctttccatt ggggtactaa catagccatc aaatgccggc ccgtgtttaa tctcctcgag
     9001 atggctgggc tcacatgcgt tctactcaaa aactcttata cctaggccct ctcccaatcg
     9061 tgtatgtttg gaccttcctc tctcacactt tggataacat caagcatacc tgcagatttt
     9121 gacctagtta acaaggcaca tcatgctatc acttccaaca ctcttgctga gcgcgttctg
     9181 ccttgccttt ctggttctac gaagtatcca gtcccgcctg caacgccgca gaaacgccaa
     9241 aaggtggggt tgtcaaccgc cagctagagc tccatcggga ttctttggca ttccgccgtt
     9301 cctcggtggt cgcagataac tccctaaatt ccctgagcta actctaagtc gaccatgccg
     9361 tttatggtta gcgcctccca aaaaggaatg gccgacttag agttacctct tgaccgactt
     9421 tttctttcct cccccttaca tttcgttacc acaacacatt cctatatcca aactccaggt
     9481 acataactag tcgaaatctc ttttaaatct agtcaagaac tagatactta accttcatac
     9541 tgcttagaga tagcctttga agctcttatt tcgctctttg tctcactctc accttcctcc
     9601 tcctcctcca atcctttttg ctcgggacta gtccaagact agtcaacgat tagtatgccg
     9661 agcattcgcg ataacgatct ccgaaagtcc ccagagtact gtcactatct cgaggcagtt
     9721 aaggacgggg agcttacgct gccggatttc aagatagtaa gccgacccga ctagctttaa
     9781 gcctagttac tgactagtcc acaggacgac aatggcgtgc ctgatatcca tccatatgaa
     9841 gtctactgcc gagtgaaggg atgcctcaag cgtacagtga gtctactgct atactagttt
     9901 ctgactagtt attaactagt tgacaggttc cctctgccaa cagaaacata ttggtcaagc
     9961 acttgaagga caagaactcc cacggcatgg agtttacatt gcacaatgga cctcccacta
    10021 tgaaggaact gatggaggcc aaaggcaagt cctatctaga ttacttggtg actagtccct
    10081 gactagtcaa gtagcatggt atgaaggctt gtttgaaggc actgttctcc caaccccgac
    10141 tcctaccaag aagcgcaagc gagctgcgta agtttctgtg agtctaacta gtgtattagc
    10201 taatatatag cagcaccaag tccaaggacc acaatactaa gggtgtcgag aattcgtgag
    10261 tttcttctcc catttcaact agtccttgac tagtcactaa ctactttgca gcaacgaggg
    10321 tgaagctgga aatgatcagg acaatggcga gggcccgtaa gtacagccat tcaatgcaga
    10381 ctagttgcta actaatctgt gactagttca agtggtccgt acgccgtgca tacccctgtg
    10441 actggtagga atttgagcaa gcctgtcttg ccgcgcgatg agaaaggaaa ggcaagttac
    10501 attcagcccc gtacctagga tcagtgctaa tttataacct ctagccactc tttatgcaga
    10561 tccgccgtga gggtagcaag gcagctaaat cagctggtga gaaaggaacc ataccctgca
    10621 agacctgtcg caacgcaaag ggcaaaggta agctatccaa gctagtttgg gactagattc
    10681 taactagtct cagcaccgtg tggttcaaag ccatattgcg agttttggcg ctttttctca
    10741 tcgattgacg aggcaaaggg agcgagtatg caacctcgta agtcagactc agacaaagac
    10801 caactagtta ttgaccagtc ttctagaagg ctctgttgtg gatcttgagg ccctggagag
    10861 ttcctccaac aatccggaga caagcaagtc ctcgtcggac tagtcactaa ctagactcta
    10921 actagttgca gacatggata atgcaaaaga gacaagcaat gaagaaagtg gtaagacatt
    10981 tctcctttgt ggttctggac tagtctttga ctagtcacag tcttaaacaa ggaaaatgag
    11041 catgaaaatg aggaggaaaa ggctgctgag cccgaggaag tgcagggtga tggcagacat
    11101 ggtaggttaa taccttgtta gttattgcta gtcactgact agtcaataac tagtctctga
    11161 acaccttgca atcactccgt ttgcgcagct gaacagtggt gaggataata gtagtaagtt
    11221 attctagctt cagagttata ggagactaga tactaactag tattagttgc aactaacctg
    11281 gatctcagag actttggcct caatctagaa tctatctagt tgtcaactag actgtggtat
    11341 cattgtcttt tattttccta gtcctggaac tagcttctaa ctagtctccc taatatgtgg
    11401 ctgtcttgtt tttttttttt gtttccctac ccggatatct agtccccttc taggttctgt
    11461 taacctctcg ggctctgatt tagtttaacg caaacctgag attagtttct aactagtctc
    11521 taggttttct atccaccttt aattgtaata ataaatacaa gcaacgttta tacgtcaaaa
    11581 gcatttataa acttttaccc taaagtagct tgcttgtgtg tttagtttat aattagtctc
    11641 ttattaattt gatgtaggta agcccgccac aaatatatat ttttaacaag ataccgtgga
    11701 aaaacttcgt gctatcacaa aacagtatac aaaaaataag ctggctttcc ccgtcaagct
    11761 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    11821 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    11881 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    11941 ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat
    12001 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatggggtga
    12061 cgatgagccg ctcttgcatc tttgtttgta ttatactgtc tttcttgtta cacataatta
    12121 ttctagaatg ccccaccgtt acatacggga cacagccatt tacatatgca tgtggattac
    12181 gagctaacga gttcattcaa atctcagaac tatcacataa tcatcattcc cctatcgtca
    12241 aagaccgtaa gacaaatccg gttcatgcac tgaacccatt cgggtagtga gtcatttact
    12301 cagcacactc gcgctgacgc tcgtcgaaca ccttcaatgc ctcctcggca gccttgacac
    12361 cactgagaac catggcaccg aaggtagggc ccatgcggtt aaagccatca atttcagaca
    12421 gctccatacc gccgattatc aagcccttag taacctcgcg ggtgttcttg acgatggcat
    12481 cctgcgctga ttgtgatagt tcccacttgt ccgtccgcat cggcatccgc agctcgggat
    12541 agttccgacc taggattgga tgcatgcgga accgcacgag ggcggggcgg aaattgacac
    12601 accactcctc tccacgcacc gttcaagagg tacgcgtata gagccgtata gagcagagac
    12661 ggagcacttt ctggtactgt ccgcacggga tgtccgcacg gagagccaca aacgagcggg
    12721 gccccgtacg tgctctccta ccccaggatc gcatccccgc atagctgaac atctatataa
    12781 agacccccaa ggttctcagt ctcaccaaca tcatcaacca acaatcaaca gttctctact
    12841 cagttaatta gaactcttcc aatcctatca cctcgcctta attaagcgga acaaattgaa
    12901 gcgagtcccc gtggagcgcg agcccgcgag agagtcgtgc gggatgatgc catgaagtat
    12961 cacctacatt gtatgctctc cagatcgaag aggaatactc tgaataagtg gatttaggcg
    13021 gtttctttgg tcctactata tgcacactca gattctgtca attgaaaagt cagcccgctg
    13081 tctatcatga cttctatctc cattaattgt catccagccg tgcctctgta tggtgcgtat
    13141 accctcgact cacacctgtg aagtctctgc cacttagcgc agccaaagaa gatttagtct
    13201 aaaggtctac aatcaattca ggccgtattc agggcggccg cttctgctgt ttctgggctc
    13261 aatgctttgg tatctcaaag gagaggagaa gcgtccgtgg aaggcaggaa gacctacaag
    13321 acctggcact cagaaagagg gcggttgctc ttaagtgtag ggctgcgcag gggaggaact
    13381 ctggggttgt caaatgcggc agacggaaat ctacattagc agcgtcaatc cacccccttt
    13441 tggtgaacca taaaggggct agtctacccc agcttcgaat cagaataata taaatgcggg
    13501 gaaagcttta ggtatgcgga cagatccata ctgggtcctc caagagatcg gcccgcacat
    13561 ctgacgtggt ttcaaggttc gacccatgcg gagccgcatt ggaggatcta tccctgtagg
    13621 taccttagct aaagatctac gccgtatggc caagcggaag atataggaac gatcggccat
    13681 gaagtcgtgt cttctacaaa ctctcgtata ttcactaggt tcgacgtctg ctaaactaac
    13741 ctgacctaac ctaaccttaa ctccctgcca gttcagccca tgcggacccg gcagtcatgt
    13801 ctttcgcttc agattgaacg cggctgcctc gtctcgatca ggatttagca gaagacagta
    13861 cacactgaaa aagtaccaca ataaagaagc tatattgcag gccaaatagc cgtctgctcg
    13921 gtctgtttat tcaacctggc ctctttcatc gtttttccct tttccttatt aaaccatata
    13981 tctccagcag agcgatcgcg ggaggagata atttagttga gatcgaagtt ggctttatag
    14041 ttgtaacagt gaaatcaggg cacgaatctt tagtacttcg ttgatcgcca ctccaccaag
    14101 ggtttgccat taagcataac tggcaatatt tttgtcaatt tgccttttcg accgtgtaaa
    14161 tgtccctatc catactctcc gcagtcaata tccggaggtc aagtgtgaag actgtttacc
    14221 ccagcaagat acttggacaa gcctggtttg caatccatag tttcagcagg cttcctaggt
    14281 ggccttaagc ctgggatact ggctggctgc tagcaattgc catgactatg ttggaggaaa
    14341 tgttcgagag gaatcttggg tccatgaaca gagtagtccc acttcaaatg ttgcgtatgc
    14401 ctgatattac ggaccaatag aggaacaacc tggcagcagc taacttgtat atctctaatc
    14461 gcgttgacga ccgttctgtg tcagtgcaac tgccacgcaa agacttcaga ataataaact
    14521 ttgccagaga aggcagttgt attatgttaa tatcgcaaca ttgtgaaaat taaatgaact
    14581 aacaatcaaa aagaaagaaa atccgaactt tccctccgta ttatctaacc cgtgtgcttt
    14641 tgcaacgcaa tccaactatt ccagttgacg acattaatcc aagccagcat aggaagaata
    14701 ccaaaaacct tcagacagcc gtacccgcaa gccttgttac caaccagcta tagcaatctt
    14761 acccgttcaa tttcttaatc cccatctaga cgcttccagc actctgactg tcgggagacg
    14821 ggttcctttg ccagccatga gcattcgcct agttagggag cggcggatgc ctacttactt
    14881 ctgggaacga ggcgcgccgg tgagcgggaa atatgggtat cttgggagag gagagttgga
    14941 gtagggagaa atgctggctc ggtgggagga cgagggagct ttaataatac cgctctggct
    15001 ttggtagcac tgagccagtg gtcgcagcaa aagaggggaa aggaggggaa caggtcaagc
    15061 ggagccgctc ttgttgagga tagctgttac ccatggaaat tacatgtagc aagctagcaa
    15121 cccgccgaag tgccccactg gtcttcaaga gccagtccag gtgaactgca ttccgtcccc
    15181 ttcgccgcgg tgcccactgc ctccacagcc tagatgattc tgaaaacacg ttgaagagct
    15241 cgcgataata accgaggcgg gtaatgcagg atgaaactta cgatctacaa cctacagcac
    15301 cctggttctg aaacaaagtt gaggatcccc gcgatcgata acccagaccg taatgcgaat
    15361 tgcaatttac gatttacccc ccgtagagcg gactgattct ggacctatct tgagactatg
    15421 tggtggagcc tgcgatgata accgaggccg taatgcaaaa atgaaaatta cgaaccaaga
    15481 ggcgtgatga gaagatca
//
