LOCUS       MS-Dswex2gRNA           8754 bp    DNA     circular SYN 19-Apr-2016
DEFINITION  -.
KEYWORDS    -.
SOURCE      -.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
            ORIGDB|GenBank
            VNTDATE|-11201354|
            VNTDBDATE|-11201354|
            LSOWNER|
            VNTNAME|MS-Dswex2gRNA|
            VNTAUTHORNAME|Max Scott|
            VNTAUTHOREML|mjscott3@ncsu.edu|
            VNTOAUTHORNAME|UNKNOWN|
            Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     CDS             19..696
                     /note="DsRed.T3"
     polyA_signal    712..1378
                     /note="p10 pA"
     misc_feature    complement(1398..2445)
                     /note="Dswex2gRNA"
     regulatory      complement(1684..1917)
                     /regulatory_class=""
                     /label=""
                     /note="U6:3 terminator"
     misc_feature    complement(1918..2017)
                     /label=""
                     /note="w gRNA"
     misc_feature    complement(1994..2017)
                     /label=""
                     /note="w ex2"
     regulatory      complement(2013..2432)
                     /regulatory_class=""
                     /label=""
                     /note="U6:3 promoter"
     transposon      complement(2515..2823)
                     /note="5' pBac"
     transposon      6209..6409
                     /note="3' pBac"
     misc_recomb     6467..6700
                     /note="attP220"
     promoter        6754..8751
                     /note="pUb promoter"
BASE COUNT  2497 A  1951 C  1990 G  2316 T  0 OTHER
ORIGIN
        1 GATCCACTAG TCGCCACCAT GGCCTCCTCC GAGGACGTCA TCAAGGAGTT CATGCGCTTC
       61 AAGGTGCGCA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG
      121 GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG CGGCCCCCTG
      181 CCCTTCGCCT GGGACATCCT GTCCCCCCAG TTCCAGTACG GCTCCAAGGT GTACGTGAAG
      241 CACCCCGCCG ACATCCCCGA CTACAAGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG
      301 CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG
      361 GACGGCTGCT TCATCTACAA GGTGAAGTTC ATCGGCGTGA ACTTCCCCTC CGACGGCCCC
      421 GTAATGCAGA AGAAGACTAT GGGCTGGGAG CCCTCCACCG AGCGCCTGTA CCCCCGCGAC
      481 GGCGTGCTGA AGGGCGAGAT CCACAAGGCC CTGAAGCTGA AGGACGGCGG CCACTACCTG
      541 GTGGAGTTCA AGTCCATCTA CATGGCCAAG AAGCCCGTGC AGCTGCCCGG CTACTACTAC
      601 GTGGACTCCA AGCTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAGCAGTAC
      661 GAGCGCACCG AGGGCCGCCA CCACCTGTTC CTGTAGCGGC CGCGACTCTA GAATGAATCG
      721 TTTTTAAAAT AACAAATCAA TTGTTTTATA ATATTCGTAC GATTCTTTGA TTATGTAATA
      781 AAATGTGATC ATTAGGAAGA TTACGAAAAA TATAAAAAAT ATGAGTTCTG TGTGTATAAC
      841 AAATGCTGTA AACGCCACAA TTGTGTTTGT TGCAAATAAA CCCATGATTA TTTGATTAAA
      901 ATTGTTGTTT TCTTTGTTCA TAGACAATAG TGTGTTTTGC CTAAACGTGT ACTGCATAAA
      961 CTCCATGCGA GTGTATAGCG AGCTAGTGGC TAACGCTTGC CCCACCAAAG TAGATTCGTC
     1021 AAAATCCTCA ATTTCATCAC CCTCCTCCAA GTTTAACATT TGGCCGTCGG AATTAACTTC
     1081 TAAAGATGCC ACATAATCTA ATAAATGAAA TAGAGATTCA AACGTGGCGT CATCGTCCGT
     1141 TTCGACCATT TCCGAAAAGA ACTCGGGCAT AAACTCTATG ATTTCTCTGG ACGTGGTGTT
     1201 GTCGAAACTC TCAAAGTACG CAGTCAGGAA CGTGCGCGAC ATGTCGTCGG GAAACTCGCG
     1261 CGGAAACATG TTGTTGTAAC CGAACGGGTC CCATAGCGCC AAAACCAAAT CTGCCAGCGT
     1321 CAATAGAATG AGCACGATGC CGACAATGGA GCTGGCTTGG ATAGCGATTC GAGTTAACGG
     1381 CCGGCCTTGG CGCGCCTAGA TCTATGCAAT GTATTTTATT AAAAACGGGT ATGCAATATA
     1441 ATAATCTTTT ATTGGGTATG CAACGAAAAT TTGTTTCGTC AAAGTATGCA ATATTTTTTA
     1501 TTAAAAGAGG GTATGCAATG TATTTTATTA AAAACGGGTA TGCAATAAAA AATTATTTGG
     1561 TTTCTCTAAA AAGTATGCAG CACTTATTTT TTGATAAGGT ATGCAACAAA ATTTTACTTT
     1621 GCCGAAAATA TGCAATGTTT TTGCGAATAA ATTCAACGCA CACTTATTAC GTGGCCATCT
     1681 AGTATGCATA CGCATTAAGC GAACATTAAA AAGATGTGAA AACATAACTA TTATGTCTAA
     1741 ATAAACACAC GTCAGATGTA TGTACGTCAA CGGAAAACCA TTGTCTATAT ATTACAATTA
     1801 CTAAATACAT ACCAAATTGA ATACATATTG ATGAAAAATA ATAAATACTG GCGAAAGCAA
     1861 AAAAACGAAA CATTTTTATT TTATTGAACA ACTCTCAGGC TCCAGGTAGG CAAAAAAGCA
     1921 CCGACTCGGT GCCACTTTTT CAAGTTGATA ACGGACTAGC CTTATTTTAA CTTGCTATTT
     1981 CTAGCTCTAA AACGACTGGA CTCCTTCACC GCCGAAGTTA AATTGAAAAT AGGTCTATAT
     2041 ATACGAACTG AGTCTGGAAA AAGAAGTTGA GAATTATAAA AAGTAGTGAG CACTGGCGCC
     2101 CTCTCTGCTT GGCGAGCTAA CCTTTTCGCC TCTTGGCTGA GTAGGTGGCG TTTCATTCTA
     2161 CTCTGTAAAA TTAATGTAGA ATTGAAACAC TATGGTCAAA AAATACTTAG GGGCATAAGT
     2221 TATAAAACGT ATGAAATGAA TTTTTATCAA CCTGGGCTAT TCAAAAATTT TCGAATTATT
     2281 TTATGTATTT TTTTTAATCG TTTTTCATTA TAGGTTAAAA TACACTTTAA AAGGAATTCT
     2341 TTCCTGTAAA ATAAATATAA ATAAATATGC TTTATTGACA GAAAATTTGA TGTTTTTGTA
     2401 TTTGAGTAGG AGCAATCACA GGTGAGCAAA AAAGAATTCA GATCTTAATA CGACTCACTA
     2461 TAGGGCGAAT TGGGTACCGG GCCCCCCCTC GAGGTCGACG GTATCGATAA GCTTGATATC
     2521 TATAACAAGA AAATATATAT ATAATAAGTT ATCACGTAAG TAGAACATGA AATAACAATA
     2581 TAATTATCGT ATGAGTTAAA TCTTAAAAGT CACGTAAAAG ATAATCATGC GTCATTTTGA
     2641 CTCACGCGGT CGTTATAGTT CAAAATCAGT GACACTTACC GCATTGACAA GCACGCCTCA
     2701 CGGGAGCTCC AAGCGGCGAC TGAGATGTCC TAAATGCACA GCGACGGATT CGCGCTATTT
     2761 AGAAAGAGAG AGCAATATTT CAAGAATGCA TGCGTCAATT TTACGCAGAC TATCTTTCTA
     2821 GGGTTAATCT AGCTGCATCA GGATCATATC GTCGGGTCTT TTTTCCGGCT CAGTCATCGC
     2881 CCAAGCTGGC GCTATCTGGG CATCGGGGAG GAAGAAGCCC GTGCCTTTTC CCGCGAGGTT
     2941 GAAGCGGCAT GGAAAGAGTT TGCCGAGGAT GACTGCTGCT GCATTGACGT TGAGCGAAAA
     3001 CGCACGTTTA CCATGATGAT TCGGGAAGGT GTGGCCATGC ACGCCTTTAA CGGTGAACTG
     3061 TTCGTTCAGG CCACCTGGGA TACCAGTTCG TCGCGGCTTT TCCGGACACA GTTCCGGATG
     3121 GTCAGCCCGA AGCGCATCAG CAACCCGAAC AATACCGGCG ACAGCCGGAA CTGCCGTGCC
     3181 GGTGTGCAGA TTAATGACAG CGGTGCGGCG CTGGGATATT ACGTCAGCGA GGACGGGTAT
     3241 CCTGGCTGGA TGCCGCAGAA ATGGACATGG ATACCCCGTG AGTTACCCGG CGGGCGCGCT
     3301 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC
     3361 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC
     3421 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC
     3481 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG
     3541 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
     3601 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
     3661 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
     3721 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
     3781 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
     3841 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
     3901 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
     3961 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
     4021 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
     4081 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
     4141 ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
     4201 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
     4261 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
     4321 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
     4381 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
     4441 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC
     4501 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
     4561 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC
     4621 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
     4681 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA
     4741 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG
     4801 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
     4861 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG
     4921 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
     4981 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT
     5041 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA
     5101 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
     5161 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC
     5221 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
     5281 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT
     5341 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
     5401 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
     5461 CACCTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG
     5521 CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC
     5581 CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA
     5641 CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC
     5701 ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG
     5761 GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA
     5821 GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC
     5881 CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCCCATTCGC CATTCAGGCT
     5941 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA
     6001 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG
     6061 TTGTAAAACG ACGGCCAGTG AGCGCGCCTC GTTCATTCAC GTTTTTGAAC CCGTGGAGGA
     6121 CGGGCAGACT CGCGGTGCAA ATGTGTTTTA CAGCGTGATG GAGCAGATGA AGATGCTCGA
     6181 CACGCTGCAG AACACGCAGC TAGATTAACC CTAGAAAGAT AATCATATTG TGACGTACGT
     6241 TAAAGATAAT CATGCGTAAA ATTGACGCAT GTGTTTTATC GGTCTGTATA TCGAGGTTTA
     6301 TTTATTAATT TGAATAGATA TTAAGTTTTA TTATATTTAC ACTTACATAC TAATAATAAA
     6361 TTCAACAAAC AATTTATTTA TGTTTATTTA TTTATTAAAA AAAAACAAAA ACTCAAAATT
     6421 TCTTCTATAA AGTAACAAAA CTTTTATCGA ATTCCTGCAG CCCGGGACTA GTACTGACGG
     6481 ACACACCGAA GCCCCGGCGG CAACCCTCAG CGGATGCCCC GGGGCTTCAC GTTTTCCCAG
     6541 GTCAGAAGCG GTTTTCGGGA GTAGTGCCCC AACTGGGGTA ACCTTTGAGT TCTCTCAGTT
     6601 GGGGGCGTAG GGTCGCCGAC ATGACACAAG GGGTTGTGAC CGGGGTGGAC ACGTACGCGG
     6661 GTGCTTACGA CCGTCAGTCG CGCGAGCGCG ACTAGTACAG CCCGGGGGAT CTCGAGCTCA
     6721 AGCTTCGAAT TCTGCAGTCG ACGGTACCCG ATCTTGTCGC CGGAACGCAG CGACAGAGAT
     6781 TCCAATGTGT CCGTATCTTT CAGGCTTTTG CCCTTCAGTT CCAGACGAAG CGACTGGCGA
     6841 TTCGCGTGTG GGGTCTGCTT CAGGGTCTTG TGAATTAGGG CGCGCAGATC GCCGATGGGC
     6901 GTGGCGCCGG AGGGCACCTT CACCTTGCCG TACGGCTTGC TGTTCTTCGC GTTCAAAATC
     6961 TCCAGCTCCA TTTTGCTTTC GGTGCGCTTG CAATCAGTAC TGTCCAAAAT CGAAAATCGC
     7021 CGAACCGTAG TGTGACCGTG CGGGGCTCTG CGAAAATAAA CTTTTTTAGG TATATGGCCA
     7081 CACACGGGGA AAGCACAGTG GATTATATGT TTTAATATTA TAATATGCAG GTTTTCATTA
     7141 CTTATCCAGA TGTAAGCCCA CTTAAAGCGA TTTAACAATT ATTTGCCGAA AGAGTAAAAA
     7201 CAAATTTCAC TTAAAAATGG ATTAAGAAAA GCTTGTGTAA GATTATGCGC AGCGTTGCCA
     7261 GATAGCTCCA TTTAAAACAC TTCAAAAACA ATAAGTTTTG AAAATATATA CATAAATAGC
     7321 AGTCGTTGCC GCAACGCTCA ACACATCACA CTTTTAAAAC ACCCTTTACC TACACAGAAT
     7381 TACTTTTTAA ATTTCCAGTC AAGCTGCGAG TTTCAAAATT ATAGCCGGTA GAGAAGACAG
     7441 TGCTATTTCA AAAGCAAACT AAATAAACAC CAATCCTAAC AAGCCTTGGA CTTTTGTAAG
     7501 TTTAGATCAA AGGTGGCATT GCATTCAATG TCATGGTAAG AAGTAGGTCG TCTAGGTAGA
     7561 AATCCTCATT CAGCCGGTCA AGTCAGTACG AGAAAGGTCT CAATTTGAAA TTGTCTTAAA
     7621 AATATTTTAT TGTTTTGTAC TGTGGTGAGT TTAAACGAAA AACACAAAAA AAAAGTGATA
     7681 CACAGAAATC ATAAAAAATT TTAATACAAG GTATTCGTAC GTATCAAAAA CATTTCGGCA
     7741 CAATTTTTTT TCTCTGTACT AAAGTGTTAC GAACACTACG GTATTTTTTA GTGATTTTCA
     7801 ACGGACACCG AAGGTATATA AACAGCGTTC GCGAACGGTC GCCTTCAAAA CCAATTGACA
     7861 TTTGCAGCAG CAAGTACAAG CAGAAAGTAA AGCGCAATCA GCGAAAAATT TATACTTAAT
     7921 TGTTGGTGAT TAAAGTACAA TTAAAAGAAC ATTCTCGAAA GTCACAAGAA ACGTAAGTTT
     7981 TTAACTCGCT GTTACCAATT AGTAATAAGA GCAACAAGAC GTTGAGTAAT TTCAAGAAAA
     8041 ACTGCATTTC AAGGTCTTTG TTCGGCCATT TTTTTTTTAT TCAACGCTCT ACGTAATTAC
     8101 AAAATAAGAA ATTGGCAGCC ACGCATCTTG TTTTCCCAAT CAATTGGCAT CAAAACGCAA
     8161 ACAAATCTAT AAATAAAACT TGCGTGTTGA TTTTCGCCAA GATTTATTGG CAAATTGTGA
     8221 AATTCGCAGT GACGCATTTG AAAATTCGAG AAATCACGAA CGCACTCGAG CATTTGTGTG
     8281 CATGTTATTA GTTAGTTAGT TCTTTGCTTA ATTGAAGTAT TTTACCAACG AAATCCACTT
     8341 ATTTTTAGCT GAAATAGAGT AGGTTGCTTG AAACGAAAGC CACGTCTGGA AAATTTCTTA
     8401 TTGCTTAGTA GTTGTGACGT CACCATATAC ACACAAAATA ATGTGTATGC ATGCGTTTCA
     8461 GCTGTGTATA TATACATGCA CACACTCGCA TTATGAAAAC GATGACGAGC AACGGAACAG
     8521 GTTTCTCAAC TACCTTTGTT CCTGTTTCTT CGCTTTCCTT TGTTCCAATA TTCGTAGAGG
     8581 GTTAATAGGG GTTTCTCAAC AAAGTTGGCG TCGATAAATA AGTTTCCCAT TTTTATTCCC
     8641 CAGCCAGGAA GTTAGTTTCA ATAGTTTTGT AATTTCAACG AAACTCATTT GATTTCGTAC
     8701 TAATTTTCCA CATCTCTATT TTGACCCGCA GAATAATCCA AAATGCAGAT CGGG
//
