LOCUS       Exported                7717 bp ds-DNA     circular SYN 15-APR-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 15, 2021 from SnapGene 5.2.0
            https://www.snapgene.com
COMMENT     Ligation of : pmNeonGreen-N1 Vector.xdna [4666  nt] : (BamHI[660] / 
            XhoI[613]) to Rtn3b-GFP.xdna [3043  nt] : (XhoI[613] / BamHI[3660])
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    520..589
                     /label=CMV Promoter
                     /note="""color: #00fffb; direction: RIGHT""618..3660"
                     /note="note=""Fragment extracted from Rtn3b-GFP.xdna 
                     [3043nt] : (XhoI[613] / BamHI[3660])"
                     /note="color: #84a4c0; direction: RIGHT"
                     /note="color: #a6acb3"
     misc_feature    1174..1185
                     /label=FTLL
                     /note="note=""direct 
                     strandtranslation""translation=""FTLL"""
                     /note="color: #ff8040; direction: RIGHT"
                     /note="color: #a6acb3"
     misc_feature    1210..1221
                     /label=YSKY
                     /note="note=""direct 
                     strandtranslation""translation=""YSKV"""
                     /note="color: #ff8000; direction: RIGHT"
                     /note="color: #a6acb3"
     misc_feature    1303..1314
                     /label=FEVI
                     /note="note=""direct 
                     strandtranslation""translation=""FEVI"""
                     /note="color: #ff8040; direction: RIGHT"
                     /note="color: #a6acb3"
     misc_feature    1585..1596
                     /label=WDLV
                     /note="note=""direct 
                     strandtranslation""translation=""WDLV"""
                     /note="color: #ff8040; direction: RIGHT"
                     /note="color: #a6acb3"
     misc_feature    2224..2235
                     /label=FEEL
                     /note="note=""direct 
                     strandtranslation""translation=""FEEL"""
                     /note="color: #ff8000; direction: RIGHT"
                     /note="color: #a6acb3"
     misc_feature    2929..2940
                     /label=YDIL
                     /note="note=""direct 
                     strandtranslation""translation=""YDIL"""
                     /note="color: #ff8040; direction: RIGHT"
                     /note="color: #a6acb3"
     misc_feature    3091..3660
                     /label=RHD3
                     /note="color: #808000; direction: RIGHT"
                     /note="color: #a6acb3"
     CDS             3673..4383
                     /label=mNeonGreen
                     /note="color: #09761a; direction: RIGHT"
                     /note="color: #993366; direction: RIGHT"
     rep_origin      4643..5083
                     /direction=RIGHT
                     /label=F1 ori
                     /note="color: #7f7f7f"
                     /note="color: #ffff00"
     promoter        5150..5178
                     /label=Amp prom
                     /note="color: #3f3f3f; direction: RIGHT"
                     /note="color: #ffffff"
     promoter        5382..5578
                     /label=SV40 early promoter
                     /note="color: #000000; direction: RIGHT"
                     /note="color: #ffffff"
     CDS             5616..6407
                     /label=Kan/neoR
                     /note="color: #8fbc8f; direction: RIGHT"
                     /note="color: #993366; direction: RIGHT"
     rep_origin      6993..7621
                     /direction=RIGHT
                     /label=ColE1 origin
                     /note="color: #000000"
                     /note="color: #ffff00"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagat ggcggagccg tcggcggcca ctcagtccca ttccatctcc
      661 tcgtcgtcct tcggagccga gccgtccgcg cccggcggcg gcgggagccc aggagcctgc
      721 cccgccctgg ggacgaagag ctgcagctcc tcctgtgcgg agggattgag ctctctttgc
      781 tctgatgagc catcttcaga aattatgact tcttcctttc tttcatcttc tgaaatacat
      841 aacactggcc ttacaatact acatggagaa aaaagccatg tgttagggag ccagcctatt
      901 ttagccaaag aaggaaaaga ccacttggat cttctagata tgaaaaagat ggaaaagcct
      961 caggggacca gcaacaacgt atcagactct tcagtttctc ttgcagcagg agttcattgt
     1021 gaccgtcctt ctattccagc cagtttccca gagcatcctg cttttctctc aaagaaaatt
     1081 ggtcaagtgg aagagcaaat agataaagag accaagaacc caaatggggt atcaagtagg
     1141 gaggctaaaa ctgcattgga tgctgatgac agattcactt tgctgacagc ccagaaacca
     1201 cctactgagt actctaaggt agaaggcatt tatacatatt ctttgtctcc atccaaagtt
     1261 tcaggagatg atgttattga aaaggattcc cctgaatcac catttgaagt aattattgac
     1321 aaagcagcat ttgacaaaga atttaaagac tcatataagg agagcacaga tgattttggt
     1381 agctggtctg tgcacactga taaagaatca tccgaagaca tttcagagac taatgacaag
     1441 ctttttccac tgagaaataa agaggcagga cgttacccaa tgtctgcatt gctcagtagg
     1501 cagttttcac acacaaatgc agcactggaa gaggtgtcca gatgcgtgaa tgatatgcat
     1561 aactttacta acgaaatact gacttgggat ctggttcccc aagtgaaaca acagaccgat
     1621 aaatcttctg actgcatcac aaaaactaca ggacttgaca tgagtgaata taattcagaa
     1681 attccagttg taaatcttaa aactagcact catcagaaaa ctcctgtatg ttctattgat
     1741 gggagcactc ccatcactaa atcaacaggt gattgggcag aagcatctct ccagcaagaa
     1801 aatgctatta ctggaaaacc tgtacctgac tctttgaatt ccacaaaaga attcagtatc
     1861 aaaggtgtgc aaggcaatat gcagaaacag gatgacacac ttgcagaatt acctggatct
     1921 ccacctgaga aatgtgactc tttgggttct ggagtggcca cagtgaaagt ggttttacct
     1981 gatgaccacc tgaaagatga aatggactgg cagagctctg cattgggaga aatcacagaa
     2041 gctgatagtt ctggtgagtc tgatgacaca gtaatagagg acatcacagc agatacatca
     2101 tttgaaaata acaaaattca ggctgaaaaa cctgtttcca ttccaagtgc tgttgtaaaa
     2161 acaggtgaaa gagaaatcaa agagattccc agttgtgaga gagaagaaaa aacatctaaa
     2221 aactttgaag aattggtcag tgactctgag ctgcatcaag atcagcctga tattcttgga
     2281 aggagtccag ctagtgaggc agcatgttca aaagtacccg atacgaatgt ctccttagaa
     2341 gatgtgagtg aagttgctcc tgaaaagcct attactactg agaaccccaa acttccttca
     2401 acagtgtctc caaatgtttt taatgagaca gaattctcat taaatgtgac aacatctgcc
     2461 tatttggagt cattacatgg gaaaaatgtt aaacatatag atgattcctc cccagaggac
     2521 ctgatagcag cctttacaga aaccagagat aaaggaatag tagatagtga aagaaatgct
     2581 tttaaagcaa tatcagagaa gatgacagac tttaaaacaa ctcctcctgt agaagtctta
     2641 catgaaaatg agtccggtgg ttctgaaatt aaagacattg gaagcaaata cagtgaacaa
     2701 agcaaagaaa caaatggaag tgagcctcta ggtgttttcc ctacccaagg tactccagta
     2761 gcatctcttg acttagaaca agaacagctc acaattaagg ctcttaaaga attaggtgaa
     2821 agacaggttg agaagtcaac ttctgcacag cgtgacgcag aattgccttc tgaagaagta
     2881 ctgaagcaaa ctttcacatt tgctccagaa tcttggccac agagatcata tgacatccta
     2941 gaacgtaatg tcaagaatgg atctgatctt gggatttccc agaagcccat cactatcaga
     3001 gaaactacta gggtagatgc tgtttccagc cttagcaaga ctgaattggt aaaaaagcat
     3061 gtcctagcaa gacttctgac agacttctca gtgcacgatc tgattttctg gagagatgtg
     3121 aagaagactg ggtttgtctt tggcaccacg ctgatcatgc tgctttccct ggcagctttc
     3181 agtgtcatca gtgtggtttc ttacctcatc ctggctcttc tctctgtcac catcagcttc
     3241 aggatctaca agtccgtcat ccaagctgta cagaagtcag aagaaggcca tccattcaaa
     3301 gcctacctgg acgtagacat tactctgtcc tcagaagctt tccataatta catgaatgct
     3361 gccatggtgc acatcaacag ggccctgaaa ctcattattc gtctctttct ggtagaagat
     3421 ctggttgact ccttgaagct ggctgtcttc atgtggctga tgacctatgt tggtgctgtt
     3481 tttaacggaa tcacccttct aattcttgct gaactgctca ttttcagtgt cccgattgtc
     3541 tatgagaagt acaagaccca gattgatcac tatgttggca tcgcccgaga tcagaccaag
     3601 tcaattgttg aaaagatcca agcaaaactc cctggaatcg ccaaaaaaaa ggcagaacgg
     3661 gatccaccgg tcatggtgag caagggcgag gaggataaca tggcctctct cccagcgaca
     3721 catgagttac acatctttgg ctccatcaac ggtgtggact ttgacatggt gggtcagggc
     3781 accggcaatc caaatgatgg ttatgaggag ttaaacctga agtccaccaa gggtgacctc
     3841 cagttctccc cctggattct ggtccctcat atcgggtatg gcttccatca gtacctgccc
     3901 taccctgacg ggatgtcgcc tttccaggcc gccatggtag atggctccgg ctaccaagtc
     3961 catcgcacaa tgcagtttga agatggtgcc tcccttactg ttaactaccg ctacacctac
     4021 gagggaagcc acatcaaagg agaggcccag gtgaagggga ctggtttccc tgctgacggt
     4081 cctgtgatga ccaactcgct gaccgctgcg gactggtgca ggtcgaagaa gacttacccc
     4141 aacgacaaaa ccatcatcag tacctttaag tggagttaca ccactggaaa tggcaagcgc
     4201 taccggagca ctgcgcggac cacctacacc tttgccaagc caatggcggc taactatctg
     4261 aagaaccagc cgatgtacgt gttccgtaag acggagctca agcactccaa gaccgagctc
     4321 aacttcaagg agtggcaaaa ggcctttacc gatgtgatgg gcatggacga gctgtacaag
     4381 taagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     4441 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     4501 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4561 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4621 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     4681 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     4741 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     4801 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     4861 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     4921 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     4981 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     5041 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     5101 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5161 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     5221 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     5281 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     5341 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5401 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5461 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5521 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     5581 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     5641 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     5701 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     5761 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     5821 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     5881 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     5941 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     6001 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     6061 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     6121 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     6181 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     6241 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     6301 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     6361 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     6421 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     6481 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     6541 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     6601 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     6661 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     6721 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     6781 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     6841 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     6901 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6961 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7021 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7081 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7141 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     7201 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7261 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7321 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7381 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7441 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7501 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7561 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7621 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     7681 ccctgattct gtggataacc gtattaccgc catgcat
//
