LOCUS       3xmnx1_full_leng        8730 bp    DNA     circular UNA 18-MAY-2021
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local...416-81z47kw
VERSION     urn.local...416-81z47kw
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1204..1225
                     /note="Geneious type: attB4"
                     /standard_name="attB4"
     promoter        1243..1653
                     /standard_name="3x125bp"
     misc_feature    1669..1690
                     /note="Geneious type: attB1"
                     /standard_name="attB1"
     promoter        1698..1830
                     /standard_name="globin enhancer"
     gene            1843..2577
                     /standard_name="mCherry"
     gene            2576..3582
                     /note="Geneious type: Gene"
                     /standard_name="PA-Rac"
     gene            2578..3012
                     /standard_name="LOV Domain"
     variation       complement(2914..2915)
                     /standard_name="L514K"
     variation       2965..2966
                     /standard_name="L531E"
     gene            3013..3582
                     /standard_name="Rac1"
     misc_feature    complement(3601..3622)
                     /note="Geneious type: attB2"
                     /standard_name="attB2"
     gene            3665..3789
                     /standard_name="SV40 poly A"
     misc_feature    complement(3821..3842)
                     /note="Geneious type: attB3"
                     /standard_name="attB3"
     gene            complement(7746..8606)
                     /standard_name="Ampicillin"
ORIGIN      
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccgggctg gctaagaact catcagcctc
      361 cccggtccat ctacccacgt accaatgcac caattggcca caatgacggc tactacatgg
      421 tgccattcct tcctctttat aggaatggag actacctcct gtccaacaag gctcttggat
      481 acgagtacgc ctacctgttg gacccaggtc attgcacaac accagaaatg ccctctgatc
      541 tgcaaaagac gtgaatatct gttcagacac ccatatccac tctgttccac acaggtcaga
      601 ggtttgtcca ggagttcttg acagaggtgt aaaaagtact caaaaatttt actcaagtga
      661 aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct ggctagaatc
      721 ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat taaggaagta
      781 aaagtaaaag caagaaagaa aactagagat tcttgtttaa gcttttaatc tcaaaaaaca
      841 ttaaatgaaa tgcatacaag gttttatcct gctttagaac tgtttgtatt taattatcaa
      901 actataagac agacaatcta atgccagtac acgctactca aagttgtaaa acctcagatt
      961 taacttcagt agaagctgat tctcaaaatt gttagtgtca agcctagctc ttttggggct
     1021 gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg gaagaggtgt
     1081 attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact ccctggtgtc
     1141 tgaaacacag gccagatggg ccctcgagca ggaaacagct atgaccatga ttacgccaag
     1201 ctatcaactt tgtatagaaa agttggctcc gaattcgccc tttcctcgag agtggttagc
     1261 tgatgaattg aggaaaacta atccgcttta ttggtagaca ggttaagggc aacagagtgt
     1321 caataattct ctccctgacc tctttctcca ttagcttaaa gaggcttata agagggcatg
     1381 ccgagagtgg ttagctgatg aattgaggaa aactaatccg ctttattggt agacaggtta
     1441 agggcaacag agtgtcaata attctctccc tgacctcttt ctccattagc ttaaagaggc
     1501 ttataagagg cctaggaaaa ggagtggtta gctgatgaat tgaggaaaac taatccgctt
     1561 tattggtaga caggttaagg gcaacagagt gtcaataatt ctctccctga cctctttctc
     1621 cattagctta aagaggctta taagaggact tgtaagggcg aattcgaccc aagtttgtac
     1681 aaaaaagcag gctccgctgg ccaatctgct cagagaggac agagtgggca ggagccagca
     1741 ttgggtatat aaagctgagc agggtcagtt gcttcttacg tttgcttctg attctgttgt
     1801 gttgacttgc aacctcagaa acagacatcc gcccccttca ccatggcaca ccatcaccac
     1861 catcacggat ctgtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     1921 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1981 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     2041 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     2101 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     2161 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     2221 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     2281 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     2341 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     2401 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     2461 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     2521 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggga
     2581 tccttggcta ctacacttga acgtattgag aagaactttg tcattactga cccaaggttg
     2641 ccagataatc ccattatatt cgcgtccgat agtttcttgc agttgacaga atatagccgt
     2701 gaagaaattt tgggaagaaa ctgcaggttt ctacaaggtc ctgaaactga tcgcgcgaca
     2761 gtgagaaaaa ttagagatgc catagataac caaacagagg tcactgttca gctgattaat
     2821 tatacaaaga gtggtaaaaa gttctggaac ctctttcact tgcagcctat gcgagatcag
     2881 aagggagatg tccagtactt tattggggtt cagaaggatg gaactgagca tgtccgagat
     2941 gctgccgaga gagagggagt catggagatt aagaaaactg cagaaaatat tgatgaggcg
     3001 gcaaaagaac ttatcaagtg tgtggtggtg ggagacggag ctgtaggtaa aacttgccta
     3061 ctgatcagtt acacaaccaa tgcatttcct ggagaatata tccctactgt ctttgacaat
     3121 tattctgcca atgttatggt agatggaaaa ccggtgaatc tgggcttatg ggatacagct
     3181 ggactagaag attatgacag attacgcccc ctatcctatc cgcaaacaga tgtgttctta
     3241 atttgctttt cccttgtgag tcctgcatca ttccaccacg tccgtgcaaa gtggtatcct
     3301 gaggtgcggc accactgtcc caacactccc atcatcctag tgggaactaa acttgatctt
     3361 agggatgata aagacacgat cgagaaactg aaggagaaga agctgactcc catcacctat
     3421 ccgcagggtc tagccatggc taaggagatt ggtgctgtaa aatacctgga gtgctcggcg
     3481 ctcacacagc gaggcctcaa gacagtgttt gacgaagcga tccgagcagt cctctgcccg
     3541 cctcccgtga agaagaggaa gagaaaatgc ctgctgttgt aaaagggtgg gcgcgccgac
     3601 ccagctttct tgtacaaagt gggggatcca gacatgataa gatacattga tgagtttgga
     3661 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     3721 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     3781 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt ccaactttat tatacatagt
     3841 tgataattca ctggccgtcg ttttacggta ccatcgatga tgatccagac atgataagat
     3901 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     3961 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     4021 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     4081 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagat cagatctctt
     4141 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4201 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4261 tgtctggatc tacgtaatac gactcactat agttctagag gctcgagagg ggccgcttta
     4321 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
     4381 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg
     4441 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
     4501 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
     4561 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
     4621 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
     4681 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
     4741 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
     4801 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt
     4861 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
     4921 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
     4981 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt
     5041 cactgtctgc tttgctgttg gtctgggctc ctgggtcact ggcttactaa tggagtcttt
     5101 atgtatgagg actcttatca attgttcttc tataaaggtc tgcagtgttt ctgttcgtcc
     5161 cctacatgga cacccagagc ctcctaaata caggagccct gataactgca caagtgctca
     5221 gattccagca gggtggaaaa tgagataaag tgtgcagatg gggaggggga cgtgaatgag
     5281 agatttgagg gatgaaaagg atggatgaac gcattgaaaa tagccccttt cacaaaataa
     5341 taccagtaaa ttgtcatata attaatgacc agacctttac ctgtaaatga tgtgatgttc
     5401 atatacagaa gaatgccaac aatttacaga tgattttaca aattcttaat tttccttttc
     5461 taaatggatt tgttcacaca ttatctcttc aaagcaattg aattttctgg aaaagattgg
     5521 ctgtgtgtgt aaaaggtgct actgaggaat ttaatgtgac atggaatgaa gcaaacagca
     5581 gcctaagaca ggatggcagg aaaaatgtca catgtttaaa ataagagtga aaccaacgac
     5641 ctggacttat agagactgtg tgtccttagg caaagctctt gtacaaacag tgcttgtgac
     5701 tgtgaaattc ggggtttgcc tggattgtgt taaatgtgtg cttggatgta tcataaaaaa
     5761 gaatcggttc tctgtatttg acattttctg taatttatct aaagttacaa ctggctatgc
     5821 ctgattttta tcactattga atcaatcatt ttaaagaatg ttaagtctgc tgatttggag
     5881 agctcattta catttatttt gaatgtctct tatttaacac aactgattta agctttagat
     5941 ctgcgaagat acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc
     6001 gcagctgctg atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt
     6061 tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact
     6121 tccaaaggac caatgaacat gtctgaccaa tttcatataa tgtgaaaacg attttcatag
     6181 gcagaataaa taacatttaa attaaactgg gcatcagcgc aattcaattg gtttggtaat
     6241 agcaagggaa aatagaatga agtgatctcc aaaaaataag tactttttga ctgtaaataa
     6301 aattgtaagg agtaaaaagt actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa
     6361 gtattgattt ttaattgtac tcaagtaaag taaaaatccc caaaaataat acttaagtac
     6421 agtaatcaag taaaattact caagtacttt acacctctgg ttcttgaccc cctaccttca
     6481 gcaagcccag cagatccact agttctagag cggccgccac cgcggtggag ctccagcttt
     6541 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     6601 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6661 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6721 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6781 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6841 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6901 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6961 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7021 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7081 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7141 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7201 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7261 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7321 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7381 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     7441 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7501 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7561 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7621 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7681 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7741 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7801 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7861 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7921 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7981 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8041 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8101 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8161 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8221 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8281 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8341 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8401 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8461 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8521 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8581 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8641 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8701 ggggttccgc gcacatttcc ccgaaaagtg
//
