LOCUS       pME-ReaChR              4469 bp ds-DNA     circular SYN 18-MAY-2021
DEFINITION  synthetic DNA.
ACCESSION
VERSION     .
KEYWORDS    NEBuilder.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   .
  TITLE     NEBuilder-generated Construct
  JOURNAL   Exported 26-FEB-2019 from NEBuilder https://nebuilder.neb.com
COMMENT     NEBuilder-generated oligos (UPPERCASE = gene-specific, lowercase =
            overlap)COMMENT     Fragment_fwd:
            cagttccctactctcgcgttAACGCTAGCATGGATGTTTTCCCCOMMENT     Fragment_fwd
            3'Tm: 67.8  3'Ta: 68.8                     COMMENT    
            Fragment_rev: tgctcaccatGGTGAAGGGGGCGGATGT              COMMENT    
            Fragment_rev 3'Tm: 70.9  3'Ta: 68.8                     COMMENT    
            ReaChR_fwd: ccccttcaccATGGTGAGCAGAAGACCC                COMMENT    
            ReaChR_fwd 3'Tm: 64.3  3'Ta: 63.7                       COMMENT    
            ReaChR_rev: gtacaagaaagctgggtcggCTTGTACAGCTCGTCCATG     COMMENT    
            ReaChR_rev 3'Tm: 62.7  3'Ta: 63.7.
FEATURES             Location/Qualifiers
     misc_feature    complement(6..103)
                     /note="Geneious type: attL2"
                     /standard_name="attL2"
     gene            273..1082
                     /gene="aph(3')-Ia"
                     /standard_name="Kanamycin"
     misc_feature    2447..2544
                     /note="Geneious type: attL1"
                     /standard_name="attL1"
     promoter        2552..2684
                     /standard_name="Globin Enhancer"
     gene            2697..3752
                     /note="ReaChR"
                     /standard_name="ReaChR"
     gene            3753..4469
                     /standard_name="Citrine"
ORIGIN      
        1 ccgacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
       61 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
      121 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
      181 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
      241 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
      301 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
      361 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
      421 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
      481 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
      541 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
      601 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
      661 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
      721 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
      781 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
      841 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
      901 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
      961 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     1021 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     1081 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     1141 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     1201 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1261 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1321 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     1381 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1441 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1501 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1561 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1621 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1681 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1741 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1801 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1861 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     1921 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     1981 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2041 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     2101 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     2161 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     2221 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     2281 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     2341 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
     2401 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt
     2461 tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa
     2521 tgccaacttt gtacaaaaaa gcaggctccg ctggccaatc tgctcagaga ggacagagtg
     2581 ggcaggagcc agcattgggt atataaagct gagcagggtc agttgcttct tacgtttgct
     2641 tctgattctg ttgtgttgac ttgcaacctc agaaacagac atccgccccc ttcaccatgg
     2701 tgagcagaag accctggctg ctggccctgg ccctggccgt ggccctggcc gccggcagcg
     2761 ccggcgccag caccggcagc gacgccaccg tgcccgtggc cacccaggac ggccccgact
     2821 acgtgttcca cagagcccac gagagaatgc tgttccagac cagctacacc ctggagaaca
     2881 acggcagcgt gatctgcatc cccaacaacg gccagtgctt ctgcctggcc tggctgaaga
     2941 gtaacggcac caacgccgag aagctggccg ccaacatcct gcagtgggtg gtgtttgcgc
     3001 tgagcgtggc gtgcctgggc tggtatgcgt atcaggcgtg gcgcgcgacc tgcggctggg
     3061 aagaagtgta tgtggcgctg attgaaatga tgaaaagcat tattgaagcg tttcatgaat
     3121 ttgatagccc ggcgaccctg tggctgagca gcggcaacgg cgtggtgtgg atgcgctatg
     3181 gcgaatggct gctgacctgc cccgtgattc tgattcatct gagcaacctg accggcctga
     3241 aagatgatta tagcaaacgc accatgggcc tgctggtgag cgacgtgggc tgcattgtgt
     3301 ggggcgcgac cagcgcgatg tgcaccggct ggaccaaaat tctgtttttt ctgattagcc
     3361 tgagctatgg catgtatacc tattttcatg cggccaaagt gtatattgaa gcgtttcata
     3421 ccgtgccgaa aggcctgtgc agacagctgg tgagagccat ggcctggctg ttcttcgtga
     3481 gctggggcat gttccccgtg ctgttcctgc tgggccccga gggcttcggc catattagcc
     3541 cgtatggcag cgcgattggc catagcattc tggatctgat tgcgaagaac atgtggggcg
     3601 tgctgggcaa ctatctgcgc gtgaaaattc atgaacatat tctgctgtat ggcgatattc
     3661 gcaaaaaaca gaaaattacc attgcgggcc aggaaatgga agtggaaacc ctggtggcgg
     3721 aagaagaaga taagtacgag agcagcctcg agatggtgag caagggcgag gagctgttca
     3781 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3841 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3901 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctga
     3961 tgtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4021 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4081 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     4141 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     4201 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     4261 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4321 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca
     4381 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     4441 tcactctcgg catggacgag ctgtacaag
//
