LOCUS       Exported                5169 bp ds-DNA     circular SYN 15-APR-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 15, 2021 from SnapGene 5.2.0
            https://www.snapgene.com
COMMENT     Ligation of : SNAP-C1.txt.xdna [4509  nt] : (BamHI[1219] / 
            XhoI[1172]) to BFP-Rab5.txt.xdna [652  nt] : (XhoI[1319] / 
            BamHI[1975])
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    613..1158
                     /label=SNAP
                     /note="color: #800040; direction: RIGHT"
                     1177..1828
                     /note="note=""Fragment extracted from BFP-Rab5.txt.xdna 
                     [652  nt] : (XhoI[1319] / BamHI[1975])"
                     /note="color: #84a4c0; direction: RIGHT"
     rep_origin      2095..2535
                     /direction=RIGHT
                     /label=F1 ori
                     /note="color: #4682b4"
     promoter        2602..2630
                     /label=Amp prom
                     /note="color: #4682b4; direction: RIGHT"
     promoter        2834..3030
                     /note="color: #6495ed; direction: RIGHT"
     misc_feature    2881..2958
                     /label=SV40 ORI
                     /note="color: #4682b4; direction: RIGHT"
     CDS             3068..3859
                     /label=Kan/neoR
                     /note="color: #8fbc8f; direction: RIGHT"
     rep_origin      4445..5073
                     /direction=RIGHT
                     /label=ColE1 origin
                     /note="color: #4682b4"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggacaa agactgcgaa atgaagcgca ccaccctgga tagccctctg
      661 ggcaagctgg aactgtctgg gtgcgaacag ggcctgcacc gtatcatctt cctgggcaaa
      721 ggaacatctg ccgccgacgc cgtggaagtg cctgccccag ccgccgtgct gggcggacca
      781 gagccactga tgcaggccac cgcctggctc aacgcctact ttcaccagcc tgaggccatc
      841 gaggagttcc ctgtgccagc cctgcaccac ccagtgttcc agcaggagag ctttacccgc
      901 caggtgctgt ggaaactgct gaaagtggtg aagttcggag aggtcatcag ctacagccac
      961 ctggccgccc tggccggcaa tcccgccgcc accgccgccg tgaaaaccgc cctgagcgga
     1021 aatcccgtgc ccattctgat cccctgccac cgggtggtgc agggcgacct ggacgtgggg
     1081 ggctacgagg gcgggctcgc cgtgaaagag tggctgctgg cccacgaggg ccacagactg
     1141 ggcaagcctg ggctgggttc cggactcaga tctcgagcta tgactagcag aagcacagct
     1201 aggcccaatg ggcaacccca ggccagcaaa atttgccagt tcaaattggt cctgctggga
     1261 gaatctgcag tgggaaagtc aagcctggta ttacgttttg tcaaagggca gttccatgag
     1321 taccaggaga gcaccattgg agcggccttc ctcacccagt ccgtttgtct agatgacaca
     1381 acagtgaagt ttgagatctg ggacacagct gggcaggagc gatatcacag cttagccccc
     1441 atgtactaca ggggtgccca agctgcaatc gtggtttacg acattactaa tcaggaaacc
     1501 tttgcccgag caaagacatg ggtgaaggaa ctacagcgac aggccagtcc tagcatcgtt
     1561 attgccctgg cagggaacaa agctgacctg gccaacaaac gtatggtgga gtatgaagag
     1621 gcccaggcat atgcagatga caacagctta ttgttcatgg agacttcagc caagacagct
     1681 atgaacgtga atgatctctt cctggcaata gctaagaagt tgccaaagag tgaaccccag
     1741 aatctgggag gtgcagcagg ccgaagccgg ggtgtggatc tccatgaaca gtcccagcag
     1801 aacaagagcc agtgttgtag caactgagga tccaccggat ctagataact gatcataatc
     1861 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     1921 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     1981 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     2041 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa
     2101 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     2161 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     2221 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     2281 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     2341 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     2401 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     2461 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     2521 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2581 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2641 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     2701 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     2761 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     2821 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     2881 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     2941 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     3001 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     3061 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     3121 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     3181 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     3241 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     3301 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     3361 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     3421 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     3481 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     3541 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     3601 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     3661 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     3721 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     3781 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     3841 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     3901 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     3961 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     4021 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     4081 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     4141 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     4201 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     4261 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     4321 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4381 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4441 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4501 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4561 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     4621 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4681 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4741 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4801 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4861 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4921 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4981 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5041 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5101 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5161 gccatgcat
//
