LOCUS       pCAG-nls-Cas9-nls-2a-Citrine-HDV 10103 bp    DNA     circular SYN 18-JUN-2024
DEFINITION  pCAG-nls-Cas9-nls-2a-Citrine-HDV.
ACCESSION   pCAG-nls-Cas9-nls-2a-Citrine-HDV
VERSION     pCAG-nls-Cas9-nls-2a-Citrine-HDV
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             complement(3005..5503)
                     /note="pLannotate"
                     /label="Cas9(C)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(5504..7105)
                     /note="pLannotate"
                     /label="Cas9(N)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(2165..2881)
                     /note="pLannotate"
                     /label="Citrine"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        complement(8558..8937)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     rep_origin      complement(982..1117)
                     /note="pLannotate"
                     /label="SV40 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(828..962)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        8968..9072
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     intron          complement(7277..8293)
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             9073..9933
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(1727..1782)
                     /note="pLannotate"
                     /label="β-globin poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(2957..3004)
                     /note="pLannotate"
                     /label="nucleoplasmin NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        1258..1288
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(8294..8556)
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="96.2"
                     /match_length="102.3"
                     /fragment="False"
                     /other="promoter"
     protein_bind    1222..1243
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    1296..1312
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     misc_RNA        complement(2010..2077)
                     /note="pLannotate"
                     /label="tracrRNA (fragment)"
                     /database="snapgene"
                     /identity="98.5"
                     /match_length="86.1"
                     /fragment="True"
                     /other="misc_RNA"
     CDS             complement(2892..2941)
                     /note="pLannotate"
                     /label="T2A (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="92.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1853..1930)
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="96.2"
                     /match_length="72.2"
                     /fragment="True"
                     /other="CDS"
     misc_RNA        complement(2129..2152)
                     /note="pLannotate"
                     /label="sTRSV HHRz (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="46.2"
                     /fragment="True"
                     /other="misc_RNA"
     CDS             772..826
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.1"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(1141..1163)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.7"
                     /fragment="True"
                     /other="promoter"
     CDS             1170..1209
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.7"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcggat ccagacatga
      841 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      901 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      961 ttagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     1021 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     1081 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     1141 tggttgctga ctaattgaga tgcggatccg ctggcacgac aggtttcccg actggaaagc
     1201 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     1261 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     1321 aggaaacagc tatgaccatg attacgccaa gctggtcgac ggtgcaccgt cgaccagctg
     1381 gtcgacggtg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1441 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1501 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1561 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1621 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1681 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1741 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1801 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct cagtggtatt
     1861 tgtgagccag ggcattggcc acaccagcca ccaccttctg ataggcagcc tgcacctgag
     1921 gagtgaattg cggccgcgtc ccattcgcca tgccgaagca tgttgcccag ccggcgccag
     1981 cgaggaggct gggaccatgc cggccaaaag caccgactcg gtgccacttt ttcaagttga
     2041 taacggacta gccttattta aacttgctat gctgtttcca gcatagctct taaacggtgt
     2101 agatcgtagc agtgcgacga gcttactcgt ttcgtcctca cggactcatc aggcactgga
     2161 attccttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
     2221 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg tagctcaggt
     2281 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
     2341 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
     2401 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
     2461 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
     2521 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
     2581 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
     2641 gcttcatgtg gtcggggtag cgggcgaagc acatcaggcc gtagccgaag gtggtcacga
     2701 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
     2761 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
     2821 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca
     2881 tggatctggg cccgggattt tcctccacgt ccccgcatgt tagaagactt cccctgccct
     2941 ctcctgatcc gatatccttt ttcttttttg cctggccggc ctttttcgtg gccgccggcc
     3001 ttttgtcgcc tcccagctga gacaggtcga tccgtgtctc gtacaggccg gtgatgctct
     3061 ggtggatcag ggtggcgtcc agcacctctt tggtgctggt gtacctcttc cggtcgatgg
     3121 tggtgtcaaa gtacttgaag gcggcagggg ctcccagatt ggtcagggta aacaggtgga
     3181 tgatattctc ggcctgctct ctgatgggct tatcccggtg cttgttgtag gcggacagca
     3241 ctttgtccag attagcgtcg gccaggatca ctctcttgga gaactcgctg atctgctcga
     3301 tgatctcgtc caggtagtgc ttgtgctgtt ccacaaacag ctgtttctgc tcattatcct
     3361 cgggggagcc cttcagcttc tcatagtggc tggccaggta caggaagttc acatatttgg
     3421 agggcagggc cagttcgttt cccttctgca gttcgccggc agaggccagc attctcttcc
     3481 ggccgttttc cagctcgaac agggagtact taggcagctt gatgatcagg tcctttttca
     3541 cttctttgta gcccttggct tccagaaagt cgatgggatt cttctcgaag ctgcttcttt
     3601 ccatgatggt gatccccagc agctctttca cactcttcag tttcttggac ttgccctttt
     3661 ccactttggc caccaccagc acagaatagg ccacggtggg gctgtcgaag ccgccgtact
     3721 tcttagggtc ccagtccttc tttctggcga tcagcttatc gctgttcctc ttgggcagga
     3781 tagactcttt gctgaagccg cctgtctgca cctcggtctt tttcacgata ttcacttggg
     3841 gcatgctcag cactttccgc acggtggcaa aatcccggcc cttatcccac acgatctccc
     3901 cggtttcgcc gtttgtctcg atcagaggcc gcttccggat ctcgccgttg gccagggtaa
     3961 tctcggtctt gaaaaagttc atgatgttgc tgtagaagaa gtacttggcg gtagccttgc
     4021 cgatttcctg ctcgctcttg gcgatcatct tccgcacgtc gtacaccttg tagtcgccgt
     4081 acacgaactc gctttccagc ttagggtact ttttgatcag ggcggttccc acgacggcgt
     4141 tcaggtaggc gtcgtgggcg tggtggtagt tgttgatctc gcgcactttg taaaactgga
     4201 aatccttccg gaaatcggac accagcttgg acttcagggt gatcactttc acttcccgga
     4261 tcagcttgtc attctcgtcg tacttagtgt tcatccggga gtccaggatc tgtgccacgt
     4321 gctttgtgat ctgccgggtt tccaccagct gtctcttgat gaagccggcc ttatccagtt
     4381 cgctcaggcc gcctctctcg gccttggtca gattgtcgaa ctttctctgg gtaatcagct
     4441 tggcgttcag cagctgccgc cagtagttct tcatcttctt cacgacctct tcggagggca
     4501 cgttgtcgct cttgccccgg ttcttgtcgc ttctggtcag caccttgttg tcgatggagt
     4561 cgtccttcag aaagctctga ggcacgatat ggtccacatc gtagtcggac agccggttga
     4621 tgtccagttc ctggtccacg tacatatccc gcccattctg caggtagtac aggtacagct
     4681 tctcgttctg cagctgggtg ttttccacgg ggtgttcttt caggatctgg ctgcccagct
     4741 ctttgatgcc ctcttcgatc cgcttcattc tctcgcggct gttcttctgt cccttctggg
     4801 tggtctggtt ctctctggcc atttcgatca cgatgttctc gggcttgtgc cggcccatca
     4861 ctttcacgag ctcgtccacc accttcactg tctgcaggat gcccttctta atggcggggc
     4921 tgccggccag attggcaatg tgctcgtgca ggctatcgcc ctggccggac acctgggctt
     4981 tctggatgtc ctctttaaag gtcaggctgt cgtcgtggat cagctgcatg aagtttctgt
     5041 tggcgaagcc gtcggacttc aggaaatcca ggattgtctt gccggactgc ttgtcccgga
     5101 tgccgttgat cagcttccgg ctcagcctgc cccagccggt gtatctccgc cgcttcagct
     5161 gcttcatcac tttgtcgtcg aacaggtggg cataggtttt cagccgttcc tcgatcatct
     5221 ctctgtcctc aaacagtgtc agggtcagca cgatatcttc cagaatgtcc tcgttttcct
     5281 cattgtccag gaagtccttg tccttgataa ttttcagcag atcgtggtat gtgcccaggg
     5341 aggcgttgaa ccgatcttcc acgccggaga tttccacgga gtcgaagcac tcgattttct
     5401 tgaagtagtc ctctttcagc tgcttcacgg tcactttccg gttggtcttg aacagcaggt
     5461 ccacgatggc ctttttctgc tcgccgctca ggaaggcggg ctttctcatt ccctcggtca
     5521 cgtatttcac tttggtcagc tcgttataca cggtgaagta ctcgtacagc aggctgtgct
     5581 tgggcagcac cttctcgttg ggcaggttct tatcgaagtt ggtcatccgc tcgatgaagc
     5641 tctgggcgga agcgcccttg tccaccactt cctcgaagtt ccagggggtg atggtttcct
     5701 cgctctttct ggtcatccag gcgaatctgc tgtttcccct ggccagaggg cccacgtagt
     5761 aggggatgcg gaaggtcagg atcttctcga tcttttcccg gttgtccttc aggaatgggt
     5821 aaaaatcttc ctgccgccgc agaatggcgt gcagctctcc caggtggatc tggtggggga
     5881 tgctgccgtt gtcgaaggtc cgctgcttcc gcagcaggtc ctctctgttc agcttcacga
     5941 gcagttcctc ggtgccgtcc atcttttcca ggatgggctt gatgaacttg tagaactctt
     6001 cctggctggc tccgccgtca atgtagccgg cgtagccgtt cttgctctgg tcgaagaaaa
     6061 tctctttgta cttctcaggc agctgctgcc gcacgagagc tttcagcagg gtcaggtcct
     6121 ggtggtgctc gtcgtatctc ttgatcatag aggcgctcag gggggccttg gtgatctcgg
     6181 tgttcactct caggatgtcg ctcagcagga tggcgtcgga caggttcttg gcggccagaa
     6241 acaggtcggc gtactggtcg ccgatctggg ccagcaggtt gtccaggtcg tcgtcgtagg
     6301 tgtccttgct cagctgcagt ttggcatcct cggccaggtc gaagttgctc ttgaagttgg
     6361 gggtcaggcc caggctcagg gcaatcaggt ttccgaacag gccattcttc ttctcgccgg
     6421 gcagctgggc gatcagattt tccagccgtc tgctcttgct cagtctggca gacaggatgg
     6481 ccttggcgtc cacgccgctg gcgttgatgg ggttttcctc gaacagctgg ttgtaggtct
     6541 gcaccagctg gatgaacagc ttgtccacgt cgctgttgtc ggggttcagg tcgccctcga
     6601 tcaggaagtg gccccggaac ttgatcatgt gggccagggc cagatagatc agccgcaggt
     6661 cggccttgtc ggtgctgtcc accagtttct ttctcaggtg gtagatggtg gggtacttct
     6721 cgtggtaggc cacctcgtcc acgatgttgc cgaagatggg gtgccgctcg tgcttcttat
     6781 cctcttccac caggaaggac tcttccagtc tgtggaagaa gctgtcgtcc accttggcca
     6841 tctcgttgct gaagatctct tgcagatagc agatccggtt cttccgtctg gtgtatcttc
     6901 ttctggcggt tctcttcagc cgggtggcct cggctgtttc gccgctgtcg aacagcaggg
     6961 ctccgatcag gttcttcttg atgctgtgcc ggtcggtgtt gcccagcacc ttgaatttct
     7021 tgctgggcac cttgtactcg tcggtgatca cggcccagcc cacagagttg gtgccgatgt
     7081 ccaggccgat gctgtacttc ttgtcggctg ctgggactcc gtggataccg accttccgct
     7141 tcttctttgg ggccatggtg gcctagcgtt taaacgttaa ttaaggcgcg cctacgcgta
     7201 cttaaggctt cgaactctcg agcaattctt tgccaaaatg atgagacagc acaataacca
     7261 gcacgttgcc caggagctgt aggaaaaaga agaaggcatg aacatggtta gcagaggctc
     7321 tagagccgcc ggtcacacgc cagaagccga accccgccct gccccgtccc ccccgaaggc
     7381 agccgtcccc ccgcggacag ccccgaggct ggagagggag aaggggacgg cggcgcggcg
     7441 acgcacgaag gccctccccg cccatttcct tcctgccggc gccgcaccgc ttcgccccgc
     7501 gcccgctaga gggggtgcgg cggcgcctcc cagatttcgg ctccgcacag atttgggaca
     7561 aaggaagtcc ctgcgccctc tcgcacgatt accataaaag gcaatggctg cggctcgccg
     7621 cgcctcgaca gccgccggcg ctccgggggc cgccgcgccc ctcccccgag ccctccccgg
     7681 cccgaggcgg ccccgccccg cccggcaccc ccacctgccg ccaccccccg cccggcacgg
     7741 cgagccccgc gccacgcccc gtacggagcc ccgcacccga agccgggccg tgctcagcaa
     7801 ctcggggagg ggggtgcagg gggggttgca gcccgaccga cgcgcccaca ccccctgctc
     7861 acccccccac gcacacaccc cgcacgcagc ctttgttccc ctcgcagccc cccccgcacc
     7921 gcggggcacc gcccccggcc gcgctcccct cgcgcacact gcggagcgca caaagccccg
     7981 cgccgcgccc gcagcgctca cagccgccgg gcagcgcgga gccgcacgcg gcgctcccca
     8041 cgcacacaca cacgcacgca ccccccgagc cgctcccccc gcacaaaggg ccctcccgga
     8101 gcccctcaag gctttcacgc agccacagaa aagaaacaag ccgtcattaa accaagcgct
     8161 aattacagcc cggaggagaa gggccgtccc gcccgctcac ctgtgggagt aacgcggtca
     8221 gtcagagccg gggcgggcgg cgcgaggcgg cggcggagcg gggcacgggg cgaaggcagc
     8281 gcgcagcgac tcccgcccgc cgcgcgcttc gctttttata gggccgccgc cgccgccgcc
     8341 tcgccataaa aggaaacttt cggagcgcgc cgctctgatt ggctgccgcc gcacctctcc
     8401 gcctcgcccc gccccgcccc gcctggcgcg cgcccccccc cccccccccg cccccatcgc
     8461 tgcacaaaat aattaaaaaa taaataaata caaaattggg ggtggggagg gggggggaga
     8521 tggggagagt gaagcagaac gtggggctca cctcgaccat ggtaatagcg atgactaata
     8581 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
     8641 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
     8701 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc
     8761 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
     8821 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
     8881 tatgaactaa tgaccccgta attgattact attaataact agtcaataat caatgtcgac
     8941 ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     9001 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     9061 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     9121 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     9181 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     9241 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     9301 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     9361 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     9421 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     9481 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     9541 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9601 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9661 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9721 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9781 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9841 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9901 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9961 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    10021 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    10081 gtagaaaaga tcaaaggatc ttc
//
