LOCUS       pET28a_pGx_NL           6043 bp    DNA        circular     18-MAR-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     3517..3536
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3610..3633
                     /locus_tag="Strep-tag"
                     /label="Strep-tag"
                     /ApEinfo_label="Strep-tag"
                     /ApEinfo_fwdcolor="#bb00bb"
                     /ApEinfo_revcolor="#8a008a"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      4605..4911
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3640..3807
                     /locus_tag="protein Gx"
                     /label="protein Gx"
                     /ApEinfo_label="protein Gx"
                     /ApEinfo_fwdcolor="#cece00"
                     /ApEinfo_revcolor="#a4a400"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      4588..5043
                     /locus_tag="M13 origin"
                     /label="M13 origin"
                     /ApEinfo_label="M13 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3838..4350
                     /locus_tag="NanoLuc"
                     /label="NanoLuc"
                     /ApEinfo_label="NanoLuc"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="#005782"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    3536..3558
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4393..4410
                     /locus_tag="His-tag"
                     /label="His-tag"
                     /ApEinfo_label="His-tag"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#4a0025"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(5139..5951)
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
       61 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      121 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
      181 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      241 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      301 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      361 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
      421 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      481 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      541 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      601 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
      661 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
      721 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      781 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
      841 atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
      901 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
      961 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     1021 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     1081 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg
     1141 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
     1201 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
     1261 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
     1321 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
     1381 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
     1441 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
     1501 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
     1561 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
     1621 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
     1681 caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc
     1741 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
     1801 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
     1861 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
     1921 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     1981 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     2041 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2101 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     2161 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     2221 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     2281 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
     2341 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     2401 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     2461 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     2521 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     2581 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
     2641 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     2701 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     2761 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     2821 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     2881 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     2941 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     3001 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     3061 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     3121 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     3181 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     3241 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     3301 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     3361 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     3421 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     3481 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat
     3541 tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat
     3601 accatgggct ggtcccatcc gcagttcgag aaaggtacca tgacatttaa actgataatc
     3661 aacggcaaaa ccttaaaagg ggagatcaca attgaggcag tcgatgccta ggaagccgag
     3721 aaaatcttta aacaatatgc taatgattat ggtattgacg gagaatggac gtatgacgat
     3781 gcgacaaaaa ctttcaccgt aactgaggaa ttcactagtg gtggaagtgg gggcagcatg
     3841 gtattcacat tggaagattt cgtgggtgat tggcgacaaa cggccggtta caatttggat
     3901 caggtgttag aacaaggggg cgtaagctcc ctgttccaga atttaggagt gagcgtgaca
     3961 cctattcagc gcattgtgct gagcggcgaa aatggtctga aaattgatat tcatgtgatc
     4021 atcccttacg aaggcctgtc tggggatcaa atgggacaga ttgaaaaaat cttcaaagta
     4081 gtttatccgg tcgacgatca tcattttaaa gtaattctgc actatgggac actcgttatc
     4141 gatggagtca cgccgaatat gatagactac ttcggtcgcc cgtacgaagg aatcgcggtt
     4201 ttcgatggaa aaaaaatcac agtaacgggc acattgtgga acgggaataa aatcatagac
     4261 gaacgcctca ttaaccctga tggatcttta ctgttccgcg tcacaattaa tggcgttaca
     4321 ggttggcgac tgtgtgaacg tattctcgca actagtggtg gaagtggggg cagccttcca
     4381 gaaacaggag gacaccacca ccaccaccac taactcgagc accaccacca ccaccactga
     4441 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     4501 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
     4561 ggaactatat ccggaTtggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     4621 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4681 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4741 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4801 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4861 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4921 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4981 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     5041 tttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5101 tacattcaaa tatgtatccg ctcatgaatt aattcttaga aaaactcatc gagcatcaaa
     5161 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     5221 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     5281 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     5341 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
     5401 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     5461 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     5521 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     5581 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     5641 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     5701 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     5761 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     5821 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     5881 tataaatcag catccatgtt ggaatttaat cgcggcctag agcaagacgt ttcccgttga
     5941 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     6001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tca 
//
