LOCUS       pET28a_pGx_NL2          6556 bp    DNA        circular     18-MAR-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     3517..3536
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3610..3633
                     /locus_tag="Strep-tag"
                     /label="Strep-tag"
                     /ApEinfo_label="Strep-tag"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#480091"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      5118..5424
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3640..3810
                     /locus_tag="pGx"
                     /label="pGx"
                     /ApEinfo_label="pGx"
                     /ApEinfo_fwdcolor="#d5d500"
                     /ApEinfo_revcolor="#9d9d00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      5101..5556
                     /locus_tag="M13 origin"
                     /label="M13 origin"
                     /ApEinfo_label="M13 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3838..4350
                     /locus_tag="NanoLuc1"
                     /label="NanoLuc1"
                     /ApEinfo_label="NanoLuc1"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="#00496c"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    3536..3558
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4369..4881
                     /locus_tag="NanoLuc2"
                     /label="NanoLuc2"
                     /ApEinfo_label="NanoLuc2"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="#005b88"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(5652..6464)
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4906..4923
                     /locus_tag="His-tag"
                     /label="His-tag"
                     /ApEinfo_label="His-tag"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#480024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
       61 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      121 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
      181 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      241 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      301 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      361 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
      421 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      481 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      541 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      601 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
      661 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
      721 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      781 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
      841 atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
      901 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
      961 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     1021 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     1081 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg
     1141 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
     1201 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
     1261 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
     1321 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
     1381 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
     1441 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
     1501 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
     1561 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
     1621 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
     1681 caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc
     1741 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
     1801 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
     1861 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
     1921 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     1981 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     2041 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2101 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     2161 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     2221 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     2281 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
     2341 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     2401 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     2461 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     2521 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     2581 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
     2641 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     2701 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     2761 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     2821 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     2881 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     2941 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     3001 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     3061 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     3121 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     3181 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     3241 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     3301 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     3361 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     3421 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     3481 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat
     3541 tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat
     3601 accatgggct ggtcccatcc gcagttcgag aaaggtacca tgacatttaa actgataatc
     3661 aacggcaaaa ccttaaaagg ggagatcaca attgaggcag tcgatgccta ggaagccgag
     3721 aaaatcttta aacaatatgc taatgattat ggtattgacg gagaatggac gtatgacgat
     3781 gcgacaaaaa ctttcaccgt aactgaggaa ttcactagtg gtggaagtgg gggcagcatg
     3841 gtcttcacgt tggaagattt cgttggagac tggcgccaga ccgctggata taacctggat
     3901 caagtgcttg agcaaggggg cgtaagttct ttgtttcaaa acctgggcgt gtctgttaca
     3961 ccgattcaga gaatcgtcct gtccggtgag aacggactga agatcgatat acacgttatc
     4021 atcccctacg aaggtttgtc gggcgatcaa atgggccaaa tcgagaagat tttcaaagtt
     4081 gtctaccccg ttgatgatca ccactttaag gtgatcctgc actacggtac acttgtcata
     4141 gacggagtca cacctaacat gatcgactac tttgggcgcc cgtacgaggg tatcgcagta
     4201 ttcgatggta aaaaaatcac agtaactgga acactttgga acggtaataa aatcatcgac
     4261 gaacgcttga taaacccaga tgggtcgctg ctgttccggg tcactataaa tggcgtgacg
     4321 ggctggagat tatgtgaacg gatattggcg gggtccggtg gatcccatat ggtctttacc
     4381 ctggaggact ttgtagggga ctggcggcag acggccgggt ataacctgga ccaggtatta
     4441 gaacagggag gcgtctcttc acttttccag aaccttgggg taagcgtaac gcctatccag
     4501 cggattgtcc tgtctgggga gaacgggtta aagatagaca tccatgtcat tatcccctac
     4561 gaaggcttgt caggcgatca gatgggccaa attgagaaga tattcaaggt agtctatcca
     4621 gtcgacgacc accattttaa ggtgatatta cactatggaa cgcttgttat cgatggagtt
     4681 actccaaata tgattgatta tttcggacgg ccctacgaag gtatagctgt ctttgacggc
     4741 aaaaagatca cggtgactgg tacgttatgg aatggaaaca aaattattga tgagcgctta
     4801 attaaccccg atggctcgct gttatttcgg gtcaccatta atggggtcac cggctggcgc
     4861 ttgtgtgaga ggattctggc gactagtggt ggaagtgggg gcagccatca tcatcatcat
     4921 cattaactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
     4981 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
     5041 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggaTt ggcgaatggg
     5101 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5161 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5221 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5281 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5341 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5401 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5461 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5521 acgcgaattt taacaaaata ttaacgttta caatttcagg tggcactttt cggggaaatg
     5581 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5641 attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     5701 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     5761 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     5821 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     5881 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca
     5941 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     6001 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
     6061 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     6121 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
     6181 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     6241 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     6301 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     6361 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     6421 aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     6481 ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac gtgagttttc
     6541 gttccactga gcgtca    
//
