LOCUS       pET28a_pGx_NL2          7087 bp    DNA        circular     18-MAR-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     3517..3536
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3610..3633
                     /locus_tag="Strep-tag"
                     /label="Strep-tag"
                     /ApEinfo_label="Strep-tag"
                     /ApEinfo_fwdcolor="#8000ff"
                     /ApEinfo_revcolor="#480091"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      5649..5955
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3640..3810
                     /locus_tag="pGx"
                     /label="pGx"
                     /ApEinfo_label="pGx"
                     /ApEinfo_fwdcolor="#d5d500"
                     /ApEinfo_revcolor="#9d9d00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      5632..6087
                     /locus_tag="M13 origin"
                     /label="M13 origin"
                     /ApEinfo_label="M13 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3838..4350
                     /locus_tag="NanoLuc1"
                     /label="NanoLuc1"
                     /ApEinfo_label="NanoLuc1"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="#00496c"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    3536..3558
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4369..4881
                     /locus_tag="NanoLuc2"
                     /label="NanoLuc2"
                     /ApEinfo_label="NanoLuc2"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="#005b88"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(6183..6995)
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4900..5412
                     /locus_tag="NanoLuc3"
                     /label="NanoLuc3"
                     /ApEinfo_label="NanoLuc3"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="#005b88"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5437..5454
                     /locus_tag="His-tag"
                     /label="His-tag"
                     /ApEinfo_label="His-tag"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#480024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
       61 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      121 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
      181 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      241 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      301 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      361 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
      421 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      481 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      541 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      601 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
      661 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
      721 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      781 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
      841 atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
      901 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
      961 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     1021 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     1081 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg
     1141 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
     1201 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
     1261 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
     1321 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
     1381 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
     1441 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
     1501 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
     1561 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
     1621 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
     1681 caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc
     1741 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
     1801 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
     1861 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
     1921 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     1981 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     2041 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2101 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     2161 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     2221 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     2281 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
     2341 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     2401 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     2461 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     2521 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     2581 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
     2641 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     2701 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     2761 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     2821 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     2881 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     2941 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     3001 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     3061 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     3121 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     3181 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     3241 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     3301 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     3361 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     3421 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     3481 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat
     3541 tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat
     3601 accatgggct ggtcccatcc gcagttcgag aaaggtacca tgacatttaa actgataatc
     3661 aacggcaaaa ccttaaaagg ggagatcaca attgaggcag tcgatgccta ggaagccgag
     3721 aaaatcttta aacaatatgc taatgattat ggtattgacg gagaatggac gtatgacgat
     3781 gcgacaaaaa ctttcaccgt aactgaggaa ttcactagtg gtggaagtgg gggcagcatg
     3841 gtcttcacgt tggaagattt cgttggagac tggcgccaga ccgctggata taacctggat
     3901 caagtgcttg agcaaggggg cgtaagttct ttgtttcaaa acctgggcgt gtctgttaca
     3961 ccgattcaga gaatcgtcct gtccggtgag aacggactga agatcgatat acacgttatc
     4021 atcccctacg aaggtttgtc gggcgatcaa atgggccaaa tcgagaagat tttcaaagtt
     4081 gtctaccccg ttgatgatca ccactttaag gtgatcctgc actacggtac acttgtcata
     4141 gacggagtca cacctaacat gatcgactac tttgggcgcc cgtacgaggg tatcgcagta
     4201 ttcgatggta aaaaaatcac agtaactgga acactttgga acggtaataa aatcatcgac
     4261 gaacgcttga taaacccaga tgggtcgctg ctgttccggg tcactataaa tggcgtgacg
     4321 ggctggagat tatgtgaacg gatattggcg gggtccggtg gatcccatat ggtgttcaca
     4381 ttagaagatt tcgtgggcga ttggagacag actgcgggtt acaacttgga tcaagtgtta
     4441 gaacaaggtg gtgtttcatc gttattccag aacttgggag taagtgtaac cccgatacaa
     4501 cgcatagtcc ttagcggtga gaatggactg aaaattgata ttcatgtaat tataccgtat
     4561 gagggattgt cgggcgacca gatgggtcaa atagaaaaga tcttcaaagt agtgtatccg
     4621 gtcgatgacc accactttaa ggtgatcttg cactatggaa ctctggtaat tgacggtgtg
     4681 acccctaata tgattgatta tttcggccgt ccgtacgagg gcatcgcagt atttgatggt
     4741 aagaagataa ccgttacggg aacattatgg aatgggaata agataatcga tgagcggttg
     4801 attaatccgg atgggtcctt actgtttcgt gtaacgataa atggagtgac tggttggcgc
     4861 ttatgtgaac gtattctggc cggggctagc ggatcacata tggtctttac cctggaggac
     4921 tttgtagggg actggcggca gacggccggg tataacctgg accaggtatt agaacaggga
     4981 ggcgtctctt cacttttcca gaaccttggg gtaagcgtaa cgcctatcca gcggattgtc
     5041 ctgtctgggg agaacgggtt aaagatagac atccatgtca ttatccccta cgaaggcttg
     5101 tcaggcgatc agatgggcca aattgagaag atattcaagg tagtctatcc agtcgacgac
     5161 caccatttta aggtgatatt acactatgga acgcttgtta tcgatggagt tactccaaat
     5221 atgattgatt atttcggacg gccctacgaa ggtatagctg tctttgacgg caaaaagatc
     5281 acggtgactg gtacgttatg gaatggaaac aaaattattg atgagcgctt aattaacccc
     5341 gatggctcgc tgttatttcg ggtcaccatt aatggggtca ccggctggcg cttgtgtgag
     5401 aggattctgg cgactagtgg tggaagtggg ggcagccatc atcatcatca tcattaactc
     5461 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
     5521 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     5581 ttgaggggtt ttttgctgaa aggaggaact atatccggaT tggcgaatgg gacgcgccct
     5641 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5701 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5761 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5821 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5881 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5941 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6001 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6061 ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa
     6121 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct
     6181 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     6241 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     6301 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     6361 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     6421 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag
     6481 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     6541 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     6601 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     6661 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     6721 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     6781 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     6841 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     6901 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     6961 ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     7021 taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7081 agcgtca     
//
