LOCUS       pAAV_DIO_EF1a_mKate_biP 7126 bp ds-DNA     circular     24-SEP-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(62..650)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(821..1612)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(1613..1681)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(1682..1786)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(2068..2523)
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     repeat_region   2598..2738
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     polyA_signal    2850..2961
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2994..3582
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             3100..3111
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    3607..3640
                     /label="loxP"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    3691..3724
                     /label="lox2272"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    3744..4451
                     /label="mKate2"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             3744..4451
                     /label="Translation 3744-4451"
     CDS             4452..4463
                     /label="Translation 4452-4463"
     CDS             4464..5549
                     /label="Translation 4464-5549"
     misc_feature    4482..5546
                     /label="biPAC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    complement(5571..5604)
                     /label="loxP"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    complement(5655..5688)
                     /label="lox2272"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(5707..6885)
                     /label="EF-1α promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     intron          5716..6654
                     /label="EF-1α intron A"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     repeat_region   6986..7126
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
       61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      481 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
      541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
      901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
      961 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1741 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1801 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1861 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     1921 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     1981 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     2041 agcagattgt actgagagtg caccataaaa ttgtaaacgt taatattttg ttaaaattcg
     2101 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     2161 cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt tggaacaaga
     2221 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     2281 atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag
     2341 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     2401 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     2461 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     2521 cgtactatgg ttgctttgac gtatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2581 ataccgcatc aggcgcccct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg
     2641 ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg
     2701 cagagaggga gtggccaact ccatcactag gggttcctgc ggccgctcgg tccgcacgtg
     2761 catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca
     2821 gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga
     2881 cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg
     2941 gctggcaact agaaggcaca gtcgagatct ctcgagcagc gctcggtatc gatgcgggga
     3001 ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg
     3061 cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta
     3121 gcagaaggac gtcccgcgca gaatccaggt ggcaacatag gcgagcagcc aaggaaagga
     3181 cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca
     3241 gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa
     3301 agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc
     3361 aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga
     3421 cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg
     3481 atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat
     3541 accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttatcgataa gcttgatatc
     3601 gaattcataa cttcgtatag catacattat acgaagttat tctttgcacc attctaaaga
     3661 ataacagtga taatttctgg gttaaggcaa ataacttcgt ataggatact ttatacgaag
     3721 ttatgctagc cggatccgcc accatggtga gcgagctgat taaggagaac atgcacatga
     3781 agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag
     3841 gcaagcccta cgagggcacc cagaccatga gaatcaaggc ggtcgagggc ggccctctcc
     3901 ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcaaaacc ttcatcaacc
     3961 acacccaggg catccccgac ttctttaagc agtccttccc cgagggcttc acatgggaga
     4021 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
     4081 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg
     4141 tgatgcagaa gaaaacactc ggctgggagg cctccaccga gaccctgtac cccgctgacg
     4201 gcggcctgga aggcagagcc gacatggccc tgaagctcgt gggcgggggc cacctgatct
     4261 gcaacttgaa gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg
     4321 tctactatgt ggacagaaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg
     4381 agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacagat
     4441 ctggactcag aagtggaagc ggtCTCGAGG CAGCTGCGAA GAAGCGGATA ACATATATTT
     4501 CCTGGGCCAA TGACCTCAGT CCCAAGCAGA TCCAACAAAT TCGAGAGGTC TCTGTAAAGA
     4561 ACAACTCCTA CAATGGTATC ACAGGAGTCC TGCTTTGTCT CAAGGGCCTG TTCTTCCAAA
     4621 TCTTGGAAGG AGACGGCGAG CGAGTAGATA AGCTCTACGA AAAGATACTG GTTGATGAAA
     4681 GGCATCGCGA TATACTGTGC CTGAAAACTG AGCTGGACGT AACGGAAAAA ATGTTCCCAG
     4741 ACTGGGGAAT GAAGACGATA AACCTGGACG AACAGGAGGG GATAATTCTT GAACCTATCA
     4801 AAAGCCTCCT CTTTACCATC GCTGAATCAC ACAGAATTCT GGAGAAATAC ACCCAACCGT
     4861 CTGTGATCAA CATAATAACG CAAGGCATTG ATCCGTTGAC GGTAGCTCCC CACATAGTGG
     4921 AAAAGATTAT TTTCTTTAGC GACATTGTAT CCTTTTCCAC ATTTAGTGAG AAGTTGCCGG
     4981 TCGATAAGGT GGTAAACTTG GTCAACCATT ATCTGACAAT TTGCACGGAG ATAATCAGCG
     5041 CACATGGTGG AGAGGTAATT AAGTTCATAG GCGATTGTGT AATGGCTGGA TTCTCTACGA
     5101 ACCAAGCAGA TGCGGCCATT AGTACAGGTC TTGAGATATT GGCTGAATTG AAAAGCCTTC
     5161 GCGAGCTGAC AGAAGTAAAT GACCCTCTTC ATTTCCTGTA CACGGGCATC GGACTCAGCT
     5221 GTGGTAATGT CATTGAAGGT AACATTGGAT CACCAGTCAA AATGGATTAC ACCCTGCTTG
     5281 GAGACGCTGT CAACGTGGCC TCACGCCTGG AAGCTCTCAG TCGGCATTTG CCCTATTCAA
     5341 TGATTCTTAG CACCGAAGTA AAAAACTACT GTTGTACCCC TTGGTCTTTC ATTAACCTCG
     5401 GGAGACACCA GATGAAGGGA AAGCAGACCC TGGTGGAGAT TTTTGCGATC AACGACTCCA
     5461 TTGTCCGCAA ATCCTCAGAC GCGAGCCAAA TTGCCAATTT CATCGCGCAG GCTCTCGATA
     5521 TGATCGCATT TAAACCACAA ATAAAGTAGA CTAGTtAGct agggcgcgcc ataacttcgt
     5581 ataatgtatg ctatacgaag ttattaagag gtttcatatt gctaatagca gctacaatcc
     5641 agctaccatt ctgcataact tcgtataaag tatcctatac gaagttattc cggaAgatcc
     5701 ggtacctcac gacacctgaa atggaagaaa aaaactttga accactgtct gaggcttgag
     5761 aatgaaccaa gatccaaact caaaaagggc aaattccaag gagaattaca tcaagtgcca
     5821 agctggccta acttcagtct ccacccactc agtgtgggga aactccatcg cataaaaccc
     5881 ctccccccaa cctaaagacg acgtactcca aaagctcgag aactaatcga ggtgcctgga
     5941 cggcgcccgg tactccgtgg agtcacatga agcgacggct gaggacggaa aggccctttt
     6001 cctttgtgtg ggtgactcac ccgcccgctc tcccgagcgc cgcgtcctcc attttgagct
     6061 ccctgcagca gggccgggaa gcggccatct ttccgctcac gcaactggtg ccgaccgggc
     6121 cagccttgcc gcccagggcg gggcgataca cggcggcgcg aggccaggca ccagagcagg
     6181 ccggccagct tgagactacc cccgtccgat tctcggtggc cgcgctcgca ggccccgcct
     6241 cgccgaacat gtgcgctggg acgcacgggc cccgtcgccg cccgcggccc caaaaaccga
     6301 aataccagtg tgcagatctt ggcccgcatt tacaagacta tcttgccaga aaaaaagcgt
     6361 cgcagcaggt catcaaaaat tttaaatggc tagagactta tcgaaagcag cgagacaggc
     6421 gcgaaggtgc caccagattc gcacgcggcg gccccagcgc ccaggccagg cctcaactca
     6481 agcacgaggc gaaggggctc cttaagcgca aggcctcgaa ctctcccacc cacttccaac
     6541 ccgaagctcg ggatcaagaa tcacgtactg cagccaggtg gaagtaattc aaggcacgca
     6601 agggccataa cccgtaaaga ggccaggccc gcgggaacca cacacggcac ttacctgtgt
     6661 tctggcggca aacccgttgc gaaaaagaac gttcacggcg actactgcac ttatatacgg
     6721 ttctccccca ccctcgggaa aaaggcggag ccagtacacg acatcacttt cccagtttac
     6781 cccgcgccac cttctctagg caccggttca attgccgacc cctcccccca acttctcggg
     6841 gactgtgggc gatgtgcgct ctgcccactg acgggcaccg gagccaattc ccactccttt
     6901 caagacctag aaggtccatt agctgcaaag attcctctct gtttaaaact ttatccatct
     6961 ttgcaaagct tacgcgtgcg gccgcaggaa cccctagtga tggagttggc cactccctct
     7021 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     7081 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcagg
//