LOCUS       pAAV_DIO_EF1a_mKate_4DC 7190 bp ds-DNA     circular     03-NOV-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(62..650)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(821..1612)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(1613..1681)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        complement(1682..1786)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(2068..2523)
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     repeat_region   2598..2738
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     polyA_signal    2850..2961
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2994..3582
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             3100..3111
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    3607..3640
                     /label="loxP"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    3691..3724
                     /label="lox2272"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    3744..4451
                     /label="mKate2"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             3744..4451
                     /label="Translation 3744-4451"
     CDS             4452..4463
                     /label="Translation 4452-4463"
     CDS             4464..5606
                     /label="Translation 4464-5606"
     misc_feature    4470..4491
                     /label="Long 5F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    4470..5606
                     /label="4D long"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4632..4650
                     /label="short 5F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4632..5549
                     /label="4D Short"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4770..5483
                     /label="CatCore"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     protein_bind    complement(5635..5668)
                     /label="loxP"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     protein_bind    complement(5719..5752)
                     /label="lox2272"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        complement(5771..6949)
                     /label="EF-1α promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     intron          5780..6718
                     /label="EF-1α intron A"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     repeat_region   7050..7190
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
       61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      481 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
      541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
      901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
      961 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1741 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1801 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1861 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     1921 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     1981 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     2041 agcagattgt actgagagtg caccataaaa ttgtaaacgt taatattttg ttaaaattcg
     2101 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     2161 cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt tggaacaaga
     2221 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     2281 atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag
     2341 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     2401 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     2461 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     2521 cgtactatgg ttgctttgac gtatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2581 ataccgcatc aggcgcccct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg
     2641 ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg
     2701 cagagaggga gtggccaact ccatcactag gggttcctgc ggccgctcgg tccgcacgtg
     2761 catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca
     2821 gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga
     2881 cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg
     2941 gctggcaact agaaggcaca gtcgagatct ctcgagcagc gctcggtatc gatgcgggga
     3001 ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg
     3061 cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta
     3121 gcagaaggac gtcccgcgca gaatccaggt ggcaacatag gcgagcagcc aaggaaagga
     3181 cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca
     3241 gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa
     3301 agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc
     3361 aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga
     3421 cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg
     3481 atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat
     3541 accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttatcgataa gcttgatatc
     3601 gaattcataa cttcgtatag catacattat acgaagttat tctttgcacc attctaaaga
     3661 ataacagtga taatttctgg gttaaggcaa ataacttcgt ataggatact ttatacgaag
     3721 ttatgctagc cggatccgcc accatggtga gcgagctgat taaggagaac atgcacatga
     3781 agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag
     3841 gcaagcccta cgagggcacc cagaccatga gaatcaaggc ggtcgagggc ggccctctcc
     3901 ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcaaaacc ttcatcaacc
     3961 acacccaggg catccccgac ttctttaagc agtccttccc cgagggcttc acatgggaga
     4021 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
     4081 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg
     4141 tgatgcagaa gaaaacactc ggctgggagg cctccaccga gaccctgtac cccgctgacg
     4201 gcggcctgga aggcagagcc gacatggccc tgaagctcgt gggcgggggc cacctgatct
     4261 gcaacttgaa gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg
     4321 tctactatgt ggacagaaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg
     4381 agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacagat
     4441 ctggactcag aagtggaagc ggtCTCGAGa tgtctcagat cagtggagtc aagaaattga
     4501 tgcacagctc tagtctgact aattcaagta tcccaaggtt tggagttaaa actgaacaag
     4561 aagatgtcct tgccaaggaa ctagaagatg tgaacaaatg gggtcttcat gttttcagaa
     4621 tagcagagtt gtctggtaac cggcccttga ctgttatcat gcacaccatt tttcaggaac
     4681 gggatttatt aaaaacattt aaaattccag tagatacttt aattacatat cttatgactc
     4741 tcgaagacca ttaccatgct gatgtggcct atcacaacaa tatccatgct gcagatgttg
     4801 tccagtctac tcatgtgcta ttatctacac ctgctttgga ggctgtgttt acagatttgg
     4861 agattcttgc agcaattttt gccagtgcaa tacatgatgt agatcatcct ggtgtgtcca
     4921 atcaatttct gatcaataca aactctgaac ttgccttgat gtacaatgat tcctcagtct
     4981 tagagaacca tcatttggct gtgggcttta aattgcttca ggaagaaaac tgtgacattt
     5041 tccagaattt gaccaaaaaa caaagacaat ctttaaggaa aatggtcatt gacatcgtac
     5101 ttgcaacaga tatgtcaaaa cacatgaatc tactggctga tttgaagact atggttgaaa
     5161 ctaagaaagt gacaagctct ggagttcttc ttcttgataa ttattccgat aggattcagg
     5221 ttcttcagaa tatggtgcac tgtgcagatc tgagcaaccc aacaaagcct ctccagctgt
     5281 accgccagtg gacggaccgg ataatggagg agttcttccg ccaaggagac cgagagaggg
     5341 aacgtggcat ggagataagc cccatgtgtg acaagcacaa tgcttccgtg gaaaaatcac
     5401 aggtgggctt catagactat attgttcatc ccctctggga gacatgggca gacctcgtcc
     5461 accctgacgc ccaggatatt ttggacactt tggaggacaa tcgtgaatgg taccagagca
     5521 caatccctca gagcccctct cctgcacctg atgacccaga ggagggccgg cagggtcaaa
     5581 ctgagaaatt ccagtttgaa ctaactTaag cttACTAGTt AGctagggcg cgccataact
     5641 tcgtataatg tatgctatac gaagttatta agaggtttca tattgctaat agcagctaca
     5701 atccagctac cattctgcat aacttcgtat aaagtatcct atacgaagtt attccggaAg
     5761 atccggtacc tcacgacacc tgaaatggaa gaaaaaaact ttgaaccact gtctgaggct
     5821 tgagaatgaa ccaagatcca aactcaaaaa gggcaaattc caaggagaat tacatcaagt
     5881 gccaagctgg cctaacttca gtctccaccc actcagtgtg gggaaactcc atcgcataaa
     5941 acccctcccc ccaacctaaa gacgacgtac tccaaaagct cgagaactaa tcgaggtgcc
     6001 tggacggcgc ccggtactcc gtggagtcac atgaagcgac ggctgaggac ggaaaggccc
     6061 ttttcctttg tgtgggtgac tcacccgccc gctctcccga gcgccgcgtc ctccattttg
     6121 agctccctgc agcagggccg ggaagcggcc atctttccgc tcacgcaact ggtgccgacc
     6181 gggccagcct tgccgcccag ggcggggcga tacacggcgg cgcgaggcca ggcaccagag
     6241 caggccggcc agcttgagac tacccccgtc cgattctcgg tggccgcgct cgcaggcccc
     6301 gcctcgccga acatgtgcgc tgggacgcac gggccccgtc gccgcccgcg gccccaaaaa
     6361 ccgaaatacc agtgtgcaga tcttggcccg catttacaag actatcttgc cagaaaaaaa
     6421 gcgtcgcagc aggtcatcaa aaattttaaa tggctagaga cttatcgaaa gcagcgagac
     6481 aggcgcgaag gtgccaccag attcgcacgc ggcggcccca gcgcccaggc caggcctcaa
     6541 ctcaagcacg aggcgaaggg gctccttaag cgcaaggcct cgaactctcc cacccacttc
     6601 caacccgaag ctcgggatca agaatcacgt actgcagcca ggtggaagta attcaaggca
     6661 cgcaagggcc ataacccgta aagaggccag gcccgcggga accacacacg gcacttacct
     6721 gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact gcacttatat
     6781 acggttctcc cccaccctcg ggaaaaaggc ggagccagta cacgacatca ctttcccagt
     6841 ttaccccgcg ccaccttctc taggcaccgg ttcaattgcc gacccctccc cccaacttct
     6901 cggggactgt gggcgatgtg cgctctgccc actgacgggc accggagcca attcccactc
     6961 ctttcaagac ctagaaggtc cattagctgc aaagattcct ctctgtttaa aactttatcc
     7021 atctttgcaa agcttacgcg tgcggccgca ggaaccccta gtgatggagt tggccactcc
     7081 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     7141 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
//