LOCUS       Exported               16409 bp ds-DNA     circular SYN 19-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 16409)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 19, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16409
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        complement(12..31)
                     /label=T3_P
     primer_bind     complement(52..68)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(57..178)
                     /label=lac Promoter
                     /note="lac Promoter"
     protein_bind    76..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    145..166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    complement(1215..2073)
                     /label=AmpR
                     /note="AmpR"
     promoter        complement(2074..2178)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2815..3270)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     3411..3427
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3434..3452
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(3491..4026)
                     /label=ScHIS3 downstream flanking (+1/+536)
     misc_feature    complement(3491..3809)
                     /label=ScHIS3 3' end
                     /note="ScHIS3 3' end"
     misc_feature    complement(4101..4542)
                     /label=MCS
                     /note="poly-linker region"
     misc_feature    complement(4101..4115)
                     /label=SGI-S1
                     /note="SGI-S1"
     misc_feature    4116..4516
                     /label=TEF1 Promoter
                     /note="TEF1 Promoter"
     misc_feature    4509..5472
                     /label=EL222mut_gblock
     misc_feature    4527..4539
                     /label=KOZAK
                     /note="KOZAK"
     misc_feature    4537..4542
                     /label=MCS
                     /note="MCS"
     CDS             4543..4563
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4564..4797
                     /label=VP16 Binding Domain
     misc_feature    4804..5433
                     
     misc_feature    4999..5001
                     /label=A79Q
     misc_feature    complement(5434..5439)
                     /label=MCS
                     /note="poly-linker region"
     misc_feature    5434..5439
                     /label=pJLA Homology Arm
     misc_feature    5446..5451
                     /label=STOP
                     /note="STOP"
     misc_feature    5452..5703
                     /label=CYC1 Terminator
                     /note="CYC1 Terminator"
     misc_feature    5704..5709
                     /label=SGI-S2
                     /note="SGI-S2"
     misc_feature    complement(5710..5948)
                     /label=MCS
                     /note="poly-linker region"
     misc_feature    5710..5714
                     /label=SGI-S1
                     /note="SGI-S1"
     misc_feature    5738..5857
                     /label=C120 Repeats
     promoter        5870..5901
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    5942..5951
                     /label=KOZAK
                     /note="KOZAK"
     misc_feature    5952..6497
                     /label=QF BD
     misc_feature    6498..6998
                     /label=QF AD
     misc_feature    6999..7004
                     /label=STOP
                     /note="STOP"
     misc_feature    7010..7011
                     /label=MCS
                     /note="MCS"
     misc_feature    7011..7016
                     /label=STOP
                     /note="STOP"
     misc_feature    7020..7312
                     /label=ACT1 t
                     /note="ACT1 t"
     misc_feature    7317..7318
                     /label=SGI-S2
                     /note="SGI-S2"
     misc_feature    7319..7333
                     /label=MCS
     misc_feature    7319..7333
                     /label=SGI-V2
                     /note="SGI-V2"
     misc_feature    7334
                     /label=Type 2
     promoter        7335..7360
                     /label=CYC1 promoter
                     /label=CYC1
                     /note="vntifkey=29"
     misc_feature    7361..7454
                     /label=5xQUAS fixed
     promoter        7456..7637
                     /label=CYC1 promoter
                     /label=CYC1
                     /note="vntifkey=29"
     TATA_signal     7461..7469
                     /label=TATA-1
                     /note="vntifkey=41"
     TATA_signal     7515..7523
                     /label=TATA-2
                     /note="vntifkey=41"
     misc_feature    complement(7644..7661)
                     /label=MCS
                     /note="poly-linker region"
     misc_feature    7646..7658
                     /label=KOZAK
                     /note="KOZAK"
     misc_feature    7662..8375
                     /label=GFP
                     /note="GFP"
     misc_feature    8382..8387
                     /label=STOP
                     /note="STOP"
     misc_feature    8391..8556
                     /label=adh1t
                     /note="adh1t"
     misc_feature    8561..8562
                     /label=SGI-S2
                     /note="SGI-S2"
     protein_bind    8567
                     /label=BsaI
                     /note="ApEinfo_fwdcolor=#00FF2C
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#E72E00"
     promoter        8568..9267
                     /label=ScCCW12 Promoter
                     /note="ApEinfo_fwdcolor=#00A1EE
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#00A1EE"
     misc_feature    complement(9270..9285)
                     /label=MCS
                     /note="poly-linker region"
     misc_feature    9276..9285
                     /label=KOZAK
                     /note="KOZAK"
     CDS             9286..12036
                     /codon_start=1
                     /label=QS
                     /translation="NTIPARHVGDVAARDPLPLPHISSSVASGMKRSFATMAMLYNDTG
                     NSNDVGAHARRPPRTLSNSRSTSAHRVPLGSWSAPNSPPRRALPHHPITASFDPDASIV
                     IAGIRGAGKSTLAIMASTAMKRKIVDLESEFHHLTGLSSSSYKKTHGPVDYGRRQIAIL
                     QNILNLHRTRAILVCSWLERDVQAMLQDFSVSNPVIYVLRDAKAIEAHLKGYDKSKVGT
                     LLDATSTVLRRCTRFEFFNVSEENLDTHSASTSPPAVPDQRHTAPYLTLKRAERHFLKF
                     LSLILPKGTIPFVESAFPLASVPVEQRRFTYALALPVSALLDKGVDIQELDVGVDAIEI
                     IVDDLATSESGPTSPLGLAPHRASEISRVVGEIRRDTVIPIILHVVFPERALYEEALLA
                     LYMTYLNHALRLAPDYLTVDLGLDSGLLGQLTTVQGTTKVIGNKQLAEVNSPRWGDPSW
                     LQAYEKAQNTGCDLVRLTRPASNPRDNTDIRQFHVAVEAVGGPRLPFIAYNTGRLGRTS
                     MCFNEILTPVTPVPTKEDAIGLRNPAHRYLQPPLTALEATQALYSAFVHDPMKLYVFGA
                     NVGYSLSPAMHNAALKACGIPHHYKPLSTANIGTLREVISDPQFAGASVGLPFKVEIIS
                     LTHSLSRHAKAIGAVNTLIPVRHLTADGGIPDEVSMFNNISQAGAVRALYGENTDWIGI
                     RACLRRGLSPANAVRSTSTGLVIGAGGMARAAVYAMLQLGVKKILIFNRTFANAEKLVL
                     HFENLLVRDALPLLSTGPRSHDNTCFHIIRSRDDPLPENFKNPTMIVSCIPTHTVDNTP
                     DPEFTVPLHWLDNPTGGIVLELDYKCLTSPLLEQTRREAHRGWVAMDGLDLLPEQGFAQ
                     FELFTGRRAPRRLMRREVLRAYPDDQAKSHTAQLQPRLNGIATQIS"
     misc_feature    12043..12054
                     /label=LINKER
                     /note="MCS"
     misc_feature    12055..12600
                     /label=PSD_V19L
     misc_feature    12601..12606
                     /label=STOP
                     /note="STOP"
     terminator      12612..12836
                     /label=ScENO1 Terminator
                     /note="ApEinfo_fwdcolor=#FF8EFF
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#FF8EFF"
     misc_feature    12843..12880
                     /label=SGI-S2
                     /note="SGI-S2"
     misc_feature    12885..13177
                     /label=ACT1 t
                     /note="ACT1 t"
     misc_feature    complement(13181..13186)
                     /label=STOP
                     /note="STOP"
     misc_feature    13186..13187
                     /label=MCS
                     /note="MCS"
     misc_feature    complement(13193..13198)
                     /label=STOP
                     /note="STOP"
     misc_feature    13199..13699
                     /label=QF AD
     misc_feature    complement(13700..14245)
                     /label=QF BD
     misc_feature    complement(14246..14255)
                     /label=KOZAK
                     /note="KOZAK"
     misc_feature    14249..14483
                     /label=MCS
                     /note="poly-linker region"
     promoter        complement(14296..14327)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    14340..14459
                     /label=C120 Repeats
     misc_feature    14483
                     /label=SGI-S1
                     /note="SGI-S1"
     misc_feature    14484..14489
                     /label=SGI-S2
                     /note="SGI-S2"
     misc_feature    14495..15908
                     /label=CG HIS3 w promoter and terminator
     misc_feature    complement(14495..15161)
                     /label=Cg HIS3 terminator
     misc_feature    complement(15162..15794)
                     /label=CgHIS3
                     /note="Candida glabrata imidazoleglycerol-phosphate 
                     dehydratase (HIS3) gene, complete cds"
     gene            15226..15855
                     /label=CgHIS3
                     /note="CgHIS3"
     misc_feature    complement(15795..15908)
                     /label=CgHIS3 promoter
     misc_feature    15872..16372
                     /label=ScHIS3 5' end
                     /note="ScHIS3 5' end"
     misc_feature    complement(15909..16372)
                     /label=ScHIS upstream flanking (-464/-1)
     promoter        complement(15909..16096)
                     /gene="S. cerevisiae HIS3"
                     /label=ScHIS3 promoter
ORIGIN
        1 cagcttttgt tccctttagt gagggttaat tccgagcttg gcgtaatcat ggtcatagct
       61 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat
      121 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      181 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      241 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
      301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1501 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1801 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2161 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcc tcggcgcgtg
     2221 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     2281 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     2341 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     2401 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     2461 tgagagtgca ccataccacc ttttcaattc atcatttttt ttttattctt ttttttgatt
     2521 tcggtttcct tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa
     2581 ggagcacaga cttagattgg tatatatacg catattgggt cagcagtaca gaaccctcga
     2641 cgaagttgta agtggtggat cagatatcat cattgttggc agaggacttt tcgccaaggg
     2701 tagagatcct aatactaaac tcacaaatta gagcttcaat ttaattatat cagttattac
     2761 cctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg
     2821 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     2881 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2941 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     3001 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     3061 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     3121 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     3181 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     3241 ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg
     3301 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     3361 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     3421 ggccagtgaa ttgtaatacg actcactata gggggccagc gtggccgttt aaacatttaa
     3481 atggccggcc gctgccaccg ttgtttccac cgaagaaacc accgttgccg taaccaccac
     3541 gacggttgtt gctaaagaag ctgccaccgc cacggccacc gttgtagccg ccgttgttgt
     3601 tattgtagtt gctactgtta tttctggcac ttcttggttt tcctcttaag tgaggaggaa
     3661 cataaccatt ctcgttgttg tcgttgatgc ttaaattttg cacttgttcg ctcagttcag
     3721 ccataatatg aaatgctttt cttgttgttc ttacggaata ccacttgcca cctatcacca
     3781 caactaactt tttcccgttc ctccatctct tttatatttt ttttctggag ttcaagagaa
     3841 aaaaaaagaa aaagcaaaaa gaaaaaagga aagcgcgcct cgttcagaat gacacgtata
     3901 gaatgatgca ttaccttgtc atcttcagta tcatactgtt cgtatacata cttactgaca
     3961 ttcataggta tacatatata cacatgtata tatatcgtat gctgcagctt taaataatcg
     4021 gtgtcaagat ctgagctcca ccgcggtggc ggccgcttat ttactcagga tcccgcatgt
     4081 tgcaggtggg agttccagct cccgggaaac agctgatagc ttcaaaatgt ttctactcct
     4141 tttttactct tccagatttt ctcggactcc gcgcatcgcc gtaccacttc aaaacaccca
     4201 agcacagcat actaaatttc ccctctttct tcctctaggg tgtcgttaat tacccgtact
     4261 aaaggtttgg aaaagaaaaa agagaccgcc tcgtttcttt ttcttcgtcg aaaaaggcaa
     4321 taaaaatttt tatcacgttt ctttttcttg aaaatttttt ttttgatttt tttctctttc
     4381 gatgacctcc cattgatatt taagttaata aacggtcttc aatttctcaa gtttcagttt
     4441 catttttctt gttctattac aacttttttt acttcttgct cattagaaag aaagcatagc
     4501 aatctaatct aagttttggc ggccgctaaa atcatggcta gcccgaaaaa aaagagaaaa
     4561 gtagccccac caacggacgt ttcacttggt gacgaattac atttagacgg tgaggatgta
     4621 gctatggcac atgccgatgc tcttgatgat tttgatctgg acatgttagg tgatggggat
     4681 tctcccggcc ccggatttac accacatgat tctgctccgt atggcgcgtt ggacatggca
     4741 gatttcgaat ttgaacagat gtttactgat gcacttggta tcgacgaata tggaggtgaa
     4801 ttcggagcag atgatactag agttgaagtc caacctccag cccaatgggt attggatttg
     4861 atcgaggcct cacccatagc aagtgttgtt agtgatccca gattggctga taatcctttg
     4921 attgctatca accaggcctt taccgatcta actgggtatt ctgaggaaga atgtgttggg
     4981 cgtaattgta ggttcttaca gggatctggt actgaaccct ggctaacaga taagatccgt
     5041 caaggtgttc gtgagcataa acccgtgttg gttgagattt tgaattacaa aaaggatggt
     5101 actccttttc gtaatgcagt tttggtggca ccaatctatg atgacgatga tgaattacta
     5161 tacttccttg gtagccaagt ggaagtggat gacgatcaac cgaacatggg catggcccgt
     5221 agagaacgtg ctgcggagat gttaaaaact ctttcaccac gtcaattaga agtcacaact
     5281 ctggttgcat caggcctaag aaataaagaa gtggctgcca gactgggtct ttcagaaaaa
     5341 actgtcaaaa tgcacagagg tttggtaatg gaaaaattaa acttaaaaac gagtgcagat
     5401 ttagttagaa ttgccgtaga agcaggtatt taagtcgacc tcgagtaatg atcatgtaat
     5461 tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag
     5521 gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa
     5581 gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta
     5641 acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg
     5701 gccttatcgc cgggtacgtg agttcgccag cttcgagtag gtagccttta gtccatgcgt
     5761 tataggtagc ctttagtcca tgcgttatag gtagccttta gtccatgcgt tataggtagc
     5821 ctttagtcca tgcgttatag gtagccttta gtccatgctt aagagacact agagggtata
     5881 taatggaagc tcgacttcca gcttggcaat ccggtactgt tggtaaagcc accgcggccg
     5941 ctaaaatcat gccacccaag agaaagacgc tgaacgcggc agcggaagct aacgcccatg
     6001 cagatggcca cgcggacggt aatgcggacg gtcatgtagc taacaccgcg gcttcctcta
     6061 acaacgcacg tttcgctgac ttgacgaata tcgatacgcc agggttggga ccgactacta
     6121 ccaccttatt ggtggagcct gccaggtcta aaagacaaag ggtcagtcgt gcgtgtgacc
     6181 aatgcagagc cgctcgtgaa aaatgtgacg gtattcagcc tgcatgtttt ccttgcgtga
     6241 gtcagggcag gtcttgcacg tatcaggctt cccccaagaa gcgtggcgtt cagactggtt
     6301 atattaggac cttggaattg gcgttggcgt ggatgtttga aaacgtagca aggtccgaag
     6361 acgcgctaca taatctatta gtcagggacg ctggccaagg ctcagcgtta ctggtaggga
     6421 aggattcacc ggcggcggaa agactgcatg caaggtgggc gacctccagg gtcaataaaa
     6481 gtatcacaag gcttctccgt caattagagc tgccgcccac ggctactgcc actgcgtcaa
     6541 tcatgcctca cgtcatggaa caacccttga gtacgtcaat aaaccctgtt aatgatcgtt
     6601 ttaatgggat tccaaatccg acgccttaca attctgacgc tgctcttgac gctataaccc
     6661 aaaccaacga ttacggttcc gtgaataccc acggaatcct gagcacctac ccccctccag
     6721 caacacacct taacgaggcg tcagtagctc tagctccggg aggggcgcct ccgcgtccac
     6781 ccccaccgta cgtggactca acgactaatc accctcccta tcatagcaac ctggtcccta
     6841 tggcaaattt tggttacagt acagttgatt atgatgccat ggtagatgat ttagcgagta
     6901 ttgaatatac tgatgctgta gatgttgacc cacaatttat gacgaatctt gggttcgtcc
     6961 ctgggtgcaa tttcagtgac attaacacgt atgaacaata atgactcgag taatgatcat
     7021 ctctgctttt gtgcgcgtat gtttatgtat gtacctctct ctctatttct atttttaaac
     7081 caccctctca ataaaataaa aataataaag tatttttaag gaaaagacgt gtttaagcac
     7141 tgactttatc tactttttgt acgttttcat tgatataatg tgttttgtct ctcccttttc
     7201 tacgaaaatt tcaaaaattg accaaaaaaa ggaatatata tacgaaaaac tattatattt
     7261 atatatcata gtgttgataa aaaatgttta tccattggac cgtgtatcat atttatcgcc
     7321 gggttatcgc cgggccagct taagaataaa ttaaactagt gggtaatcgc ttatcctagg
     7381 ataaacaatt atcctcaagg gtaatcgctt atcctctagg gtaatcgctt atcctagggt
     7441 aatcgcttat cctttctaga tatatatatg tgtgcgacga cacatgatca tatggcatgc
     7501 atgtgctctg tatgtatata aaactcttgt tttcttcttt tctctaaata ttctttcctt
     7561 atacattagg acctttgcag cataaattac tatacttcta tagacacaca aacacaaata
     7621 cacacactaa attaatagcg gccgctaaaa tcatggctag cgtcgacaaa ggagaagaac
     7681 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     7741 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
     7801 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcacttatg
     7861 gtgttcaatg cttttcaaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg
     7921 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
     7981 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     8041 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     8101 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
     8161 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     8221 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     8281 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     8341 ctgggattac acatggcatg gatgaactat acaaactcga gtaatgatca gcgaatttct
     8401 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
     8461 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
     8521 tcaggttgct ttctcaggta tagcatgagg tcgctcttat cgccgggcac ccatgaacca
     8581 cacggttagt ccaaaagggg cagttcagat tccagatgcg ggaattagct tgctgccacc
     8641 ctcacctcac taacgctgcg gtgtgcggat acttcatgct atttatagac gcgcgtgtcg
     8701 gaatcagcac gcgcaagaac caaatgggaa aatcggaatg ggtccagaac tgctttgagt
     8761 gctggctatt ggcgtctgat ttccgttttg ggaatccttt gccgcgcgcc cctctcaaaa
     8821 ctccgcacaa gtcccagaaa gcgggaaaga aataaaacgc caccaaaaaa aaaaaaataa
     8881 aagccaatcc tcgaagcgtg ggtggtaggc cctggattat cccgtacaag tatttctcag
     8941 gagtaaaaaa accgtttgtt ttggaatttc ccatttcgcg gccacctacg ccgctatctt
     9001 tgcaacaact atctgcgata actcagcaaa ttttgcatat tcgtgttgca gtattgcgat
     9061 aatgggagtc ttacttccaa cataacggca gaaagaaatg tgagaaaatt ttgcatcctt
     9121 tgcctccgtt caagtatata aagtcggcat gcttgataat ctttctttcc atcctacatt
     9181 gttctaatta ttcttattct cctttattct ttcctaacat accaagaaat taatcttctg
     9241 tcattcgctt aaacactata tcaataagcg gccgctaaaa tcatgaacac cattccagct
     9301 agacacgtcg gcgacgttgc cgccagagat cctttaccac taccccacat ctctagctcc
     9361 gtagcatctg gtatgaagcg tagcttcgca acaatggcga tgctgtataa cgacaccggc
     9421 aacagcaatg acgttggagc ccatgcgaga agacctccaa ggaccctgag taatagcagg
     9481 tcaaccagcg cgcatagagt cccgctagga agctggtccg caccgaactc acctccgagg
     9541 cgtgccttgc cacatcatcc aattaccgcg tccttcgacc ctgatgctag cattgtcatt
     9601 gcggggatca ggggggctgg gaagagcacg cttgcaatta tggcaagcac agcgatgaaa
     9661 agaaaaattg tagacttaga atctgaattc caccatttga cgggattgag tagttcaagc
     9721 tataaaaaaa cgcatggtcc tgttgattat ggcagaaggc aaatcgccat cttacaaaac
     9781 atcctgaatt tgcataggac tagagctatt ttggtatgta gttggcttga gagagatgta
     9841 caggcaatgc tgcaagactt tagtgtgagt aatcctgtca tctatgttct gagagatgcg
     9901 aaggctatcg aggcacacct gaagggatat gataagtcta aggtgggcac gcttttggac
     9961 gctacctcaa cagttcttag gaggtgcacc cgttttgaat ttttcaacgt ttcagaggaa
    10021 aacctagata cccactccgc aagtactagt cctccagctg tccccgatca gaggcacacc
    10081 gccccatatc ttaccctaaa acgtgcggaa agacactttc tgaagttttt aagtcttata
    10141 ctacccaagg ggacaatacc tttcgtagaa agtgcgttcc cgctagcctc tgtaccagtt
    10201 gaacagcgtc gtttcaccta tgctctagct ttacctgtat cagctctgct tgacaaaggg
    10261 gttgatattc aggagttgga tgttggagtc gatgccatag agattatcgt ggacgatctt
    10321 gcaacttccg aatctggacc tacaagcccc ctgggcttag ctccgcacag ggcttcagaa
    10381 ataagccgtg tcgtgggcga gattagaaga gatactgtta tacctattat ccttcatgtt
    10441 gtgttccctg agagggccct atatgaggaa gcccttctag cgttgtacat gacttatctt
    10501 aaccatgcac ttaggcttgc acccgactac ctgaccgtag accttgggct tgacagcggt
    10561 ttgttaggac agctaacaac cgtccaaggg accacgaaag tgataggaaa taaacaactt
    10621 gcagaggtaa actccccgag atggggtgat ccgagttggt tacaagcata cgagaaagca
    10681 caaaacacag ggtgtgattt agtgaggttg accagacccg ctagcaatcc ccgtgataac
    10741 acagatatta gacagttcca tgttgcagtt gaggcggttg ggggacctag gctgccattt
    10801 atcgcttata acaccgggag attgggaaga acgtctatgt gctttaatga gatcctgacc
    10861 cccgtcaccc cagtaccgac gaaggaggat gcgataggcc ttcgtaatcc ggctcacaga
    10921 tacttacagc ctcctctaac agcactggaa gccacacaag ccctttattc cgcgtttgtg
    10981 cacgatccta tgaaattata tgtgttcggg gctaacgttg gctactccct ttcaccggct
    11041 atgcacaacg cagcgctaaa agcttgcgga ataccacacc actataaacc gctatcaact
    11101 gctaatatag ggactttgag ggaagtaatt agtgatccac aatttgctgg agcttcagta
    11161 gggttaccgt tcaaagttga gattatttca cttacacata gtctgtctag acacgcaaag
    11221 gctatcggag ccgtgaatac gttaatccca gtgcgtcatc ttactgcgga cgggggcatt
    11281 ccagatgaag tcagtatgtt taataacatt tctcaggcgg gagcagtgag agctctttat
    11341 ggtgaaaata ctgattggat tggaataaga gcatgtttaa gaagggggct tagcccagca
    11401 aacgcagtga ggtctacaag caccggttta gtgataggtg ccggggggat ggcgagagcc
    11461 gcagtgtatg caatgctgca acttggggtc aaaaaaattt taattttcaa ccgtactttc
    11521 gctaatgctg aaaaactggt tcttcatttc gaaaacctgt tagttcgtga tgcacttcca
    11581 ttgttaagca cggggcccag gagtcatgat aatacgtgct ttcatatcat ccgttctaga
    11641 gatgacccat tgccagagaa tttcaaaaat cctactatga tcgtgagctg tattccaacc
    11701 catacagtcg ataacacacc ggacccggaa tttacagtcc ccctacactg gcttgacaat
    11761 cctaccggcg gtatagttct agagttagat tataagtgct taacctcccc actgctagag
    11821 cagacacgtc gtgaagctca ccgtggctgg gtggccatgg acggacttga cttactgcct
    11881 gaacaaggct ttgcccagtt cgaactgttt acaggtagac gtgcgccgcg taggttgatg
    11941 aggcgtgaag tgctacgtgc gtatccagac gaccaggcta aaagtcacac ggcacaacta
    12001 cagccccgtc taaacgggat agccacgcaa atctctctcg agagtgctgg aggacagatg
    12061 agaaagggta ttgatctagc tactacactc gaacgtatcg agaagaattt tattatcact
    12121 gatcctaggc ttcccgataa tcccattatt tttgcatctg atagtttctt ggagctcacg
    12181 gaatatagcc gtgaagaaat tcttggaaga aattgcaggt ttctacaagg tccagagact
    12241 gatctaacca cagtgaagaa gattcgaaat gctattgata accaaaccga agtgacagtt
    12301 cagctcatca actacaccaa gagcggaaag aagttctgga acattttcca cttgcaacct
    12361 atgcgtgatc agaagggaga agtacaatac tttattggag ttcaactaga cgggagcaag
    12421 cacgtagaac cagttcgcaa tgtcattgaa gaaaccgcag tgaaagaggg agaagacctg
    12481 gtgaaaaaaa cagctgtgaa tatcgatgaa gcggttcgag aacttcctat gtcttgtgca
    12541 caagaatcta ttacttcttt gtacaagaag gctggttctg aaaacttgta cttccaataa
    12601 taatgatcga gagcttttga ttaagccttc tagtccaaaa aacacgtttt tttgtcattt
    12661 atttcatttt cttagaatag tttagtttat tcattttata gtcacgaatg ttttatgatt
    12721 ctatataggg ttgcaaacaa gcatttttca ttttatgtta aaacaatttc aggtttacct
    12781 tttattctgc ttgtggtgac gcgtgtatcc gcccgctctt ttggtcaccc atgtatcgcc
    12841 ggcgaataaa ttaaggcgcg ccttaattta ttcgccggcg ataaatatga tacacggtcc
    12901 aatggataaa cattttttat caacactatg atatataaat ataatagttt ttcgtatata
    12961 tattcctttt tttggtcaat ttttgaaatt ttcgtagaaa agggagagac aaaacacatt
    13021 atatcaatga aaacgtacaa aaagtagata aagtcagtgc ttaaacacgt cttttcctta
    13081 aaaatacttt attattttta ttttattgag agggtggttt aaaaatagaa atagagagag
    13141 aggtacatac ataaacatac gcgcacaaaa gcagagatga tcattactcg agtcattatt
    13201 gttcatacgt gttaatgtca ctgaaattgc acccagggac gaacccaaga ttcgtcataa
    13261 attgtgggtc aacatctaca gcatcagtat attcaatact cgctaaatca tctaccatgg
    13321 catcataatc aactgtactg taaccaaaat ttgccatagg gaccaggttg ctatgatagg
    13381 gagggtgatt agtcgttgag tccacgtacg gtgggggtgg acgcggaggc gcccctcccg
    13441 gagctagagc tactgacgcc tcgttaaggt gtgttgctgg aggggggtag gtgctcagga
    13501 ttccgtgggt attcacggaa ccgtaatcgt tggtttgggt tatagcgtca agagcagcgt
    13561 cagaattgta aggcgtcgga tttggaatcc cattaaaacg atcattaaca gggtttattg
    13621 acgtactcaa gggttgttcc atgacgtgag gcatgattga cgcagtggca gtagccgtgg
    13681 gcggcagctc taattgacgg agaagccttg tgatactttt attgaccctg gaggtcgccc
    13741 accttgcatg cagtctttcc gccgccggtg aatccttccc taccagtaac gctgagcctt
    13801 ggccagcgtc cctgactaat agattatgta gcgcgtcttc ggaccttgct acgttttcaa
    13861 acatccacgc caacgccaat tccaaggtcc taatataacc agtctgaacg ccacgcttct
    13921 tgggggaagc ctgatacgtg caagacctgc cctgactcac gcaaggaaaa catgcaggct
    13981 gaataccgtc acatttttca cgagcggctc tgcattggtc acacgcacga ctgacccttt
    14041 gtcttttaga cctggcaggc tccaccaata aggtggtagt agtcggtccc aaccctggcg
    14101 tatcgatatt cgtcaagtca gcgaaacgtg cgttgttaga ggaagccgcg gtgttagcta
    14161 catgaccgtc cgcattaccg tccgcgtggc catctgcatg ggcgttagct tccgctgccg
    14221 cgttcagcgt ctttctcttg ggtggcatga ttttagcggc cgcggtggct ttaccaacag
    14281 taccggattg ccaagctgga agtcgagctt ccattatata ccctctagtg tctcttaagc
    14341 atggactaaa ggctacctat aacgcatgga ctaaaggcta cctataacgc atggactaaa
    14401 ggctacctat aacgcatgga ctaaaggcta cctataacgc atggactaaa ggctacctac
    14461 tcgaagctgg cgaactcacg tacccggagg tacccaaagc cgaatccacc acggtcaccg
    14521 ttgtttctga agaaaccacc acggccgcca ccgttgttga aaccaccacg aggagcagaa
    14581 tcgcctcttc tgccaccacc gttacggatg tgaggtggaa cgtaaccacc acgtctttgt
    14641 tgcccttcag gcatggaaac ttgctctgct aactcagtca tattggatat atgtattact
    14701 ttctttattt tttgtagttt agtcttctat ttcttttata gtttggtatc tttttgcaaa
    14761 aaatttacct ctatatcgtg ttcgtgcgta tttcttcctc tataatatga aaagaaaggc
    14821 agtagtcttg gttttctatt attccaaata ttacaagaat acccagaacc acaagaagca
    14881 gaagaaacta actgctttat atacttaatt tcccgccaca agctcatcgc ccagaagttt
    14941 ttttcagttt ttcacctgac aatctggcag ctcgctgaaa aattttcacc tggggtcgcc
    15001 tgctacgtag agtgatcacg tttatgaata gcacattata tagtggccca gacggtctga
    15061 tggtagttca tgtagttaag aataaatact ctgtttgtgt gatttctatg tgtacgttat
    15121 ctatataaat attgttgatg gtggtagctg tgggctgtgt tctatgctag gacaccctta
    15181 gtggatggaa cgtcatcgcg acccgtaggt gttcttgcct ctctgatggc gatggcgagc
    15241 gccttgaaag ctgattcgga gcggtggtgg tcgttggtgc cccgcagaca gtccacatgc
    15301 atggtgatac gcgcctcagt ggcgaaactc tccaagaagt gcgggatcat ctctgtggat
    15361 agctggccta tgcgttctcg cttcaggccc agctccacca cggcgaaagg cctattggag
    15421 aagtcaacca cagcgcggct cagcgcctcg tccagtggtg caaacccatg cccgaacctc
    15481 ttgataccac gcacggagcc aagcgcctcc ttgaacgctt ggcccagcgc aatcccacag
    15541 tcctcaacag tgtggtggtc atcgatgtgc aagtccccta tacactccag gatcagagac
    15601 cacccgccat gcttcgccag cgcagtcaac atgtggtcca aaaacccaac tccagtgtgc
    15661 acatggatgg tctgcccatc accactagca tattccttgc ccagtatgct ctcccgtaca
    15721 gaaacagacc caccgtcaag atccagcgcc agctgtatat tcgtctcctg cgtaaccctc
    15781 ttaacaaacg ccatcctttg ctcgatgctt ctctttgctt caattatctc aactttaagt
    15841 aaacctgaag taaactagaa ctagattaaa tcagaaggca tccgcagctt tatatacata
    15901 tagagcctct ttgccttcgt ttatcttgcc tgctcatttt ttagtatatt cttcgaagaa
    15961 atcacattac tttatataat gtataattca ttatgtgata atgccaatcg ctaagaaaaa
    16021 aaaagagtca tccgctaggt ggaaaaaaaa aaatgaaaat cattaccgag gcataaaaaa
    16081 atatagagtg tactagagga ggccaagagt aatagaaaaa gaaaattgcg ggaaaggact
    16141 gtgttatgac ttccctgact aatgccgtgt tcaaacgata cctggcagtg actcctagcg
    16201 ctcaccaagc tcttaaaacg agaattaaga aaaagtcgtc atctttcgat aagtttttcc
    16261 cacagcaaag caatagtaga aaaaaacaat gggaaacgtt gaatgaagac aaagcgtcgt
    16321 ggtttaaaag gaaatacgct cacgtacatg ctagggaaca ggaccgtgca gcggccagcg
    16381 tggccgttta aacatttaaa tggccggcc
//
