LOCUS       Exported                4107 bp ds-DNA     circular SYN 25-MAR-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 25, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_binding    3..25
                     /label=LacO
     misc_feature    57
                     /label=T or G  unknown
                     /note="note=""direct strand translation""translation=""X"""
     misc_feature    58..63
                     /label=RBS or Shine-Dalgarno
     misc_feature    74..493
                     /label=coNsp10
     promoter        602..620
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_binding    621..643
                     /label=LacO
     misc_feature    674..679
                     /label=RBS or Shine-Dalgarno
     terminator      850..897
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1127..1942)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(1943..2034)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2052..2687)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin ofreplicationcan
                     be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     CDS             complement(2885..3967)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operatortoinhibit
                     transcription in E. coli. This inhibition can be relieved 
                     by adding lactose or isopropyl-beta-D-thiogalactopyranoside
                     (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(3968..4045)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        join(4091..4107,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggcaggta atgcaaccga agttccggca aatagcaccg ttctgagctt
      121 ttgtgcattt gcagttgatg cagcaaaagc ctataaagac tatctggcaa gcggtggtca
      181 gccgattacc aattgtgtta aaatgctgtg tacccatacc ggcaccggtc aggcaattac
      241 cgttacaccg gaagcaaata tggatcaaga aagctttggt ggtgcaagct gttgtctgta
      301 ttgtcgttgt catattgatc acccgaatcc gaaaggtttt tgtgatctga aaggtaagta
      361 tgtgcagatt ccgaccacct gtgcaaatga tccggttggt tttaccctga aaaataccgt
      421 ttgcaccgtt tgtggtatgt ggaaaggtta tggttgtagc tgtgatcagc tgcgtgaacc
      481 gatgctgcag taaggatccg aattcgagct cggcgcgcct gcaggtcgac aagcttgcgg
      541 ccgcataatg cttaagtcga acagaaagta atcgtattgt acacggccgc ataatcgaaa
      601 ttaatacgac tcactatagg ggaattgtga gcggataaca attccccatc ttagtatatt
      661 agttaagtat aagaaggaga tatacatatg gcagatctca attggatatc ggccggccac
      721 gcgatcgctg acgtcggtac cctcgagtct ggtaaagaaa ccgctgctgc gaaatttgaa
      781 cgccagcaca tggactcgtc tactagcgca gcttaattaa cctaggctgc tgccaccgct
      841 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      901 aaacctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta
      961 aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac gacaagctga
     1021 cgaccgggtc tccgcaagtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     1081 atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat
     1141 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     1201 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     1261 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     1321 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     1381 atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     1441 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
     1501 gaaatacgcg gtcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     1561 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     1621 ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     1681 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
     1741 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     1801 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     1861 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg
     1921 tttcccgttg aatatggctc atactcttcc tttttcaata ttattgaagc atttatcagg
     1981 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaataggca
     2041 tgctagcgca gaaacgtcct agaagatgcc aggaggatac ttagcagaga gacaataagg
     2101 ccggagcgaa gccgtttttc cataggctcc gcccccctga cgaacatcac gaaatctgac
     2161 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2221 atggctccct cttgcgctct cctgttcccg tcctgcggcg tccgtgttgt ggtggaggct
     2281 ttacccaaat caccacgtcc cgttccgtgt agacagttcg ctccaagctg ggctgtgtgc
     2341 aagaaccccc cgttcagccc gactgctgcg ccttatccgg taactatcat cttgagtcca
     2401 acccggaaag acacgacaaa acgccactgg cagcagccat tggtaactga gaattagtgg
     2461 atttagatat cgagagtctt gaagtggtgg cctaacagag gctacactga aaggacagta
     2521 tttggtatct gcgctccact aaagccagtt accaggttaa gcagttcccc aactgactta
     2581 accttcgatc aaaccgcctc cccaggcggt tttttcgttt acagagcagg agattacgac
     2641 gatcgtaaaa ggatctcaag aagatccttt acggattccc gacaccatca ctctagattt
     2701 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     2761 aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
     2821 caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt
     2881 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     2941 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
     3001 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
     3061 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
     3121 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc gcaccaacgc
     3181 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
     3241 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
     3301 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
     3361 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
     3421 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
     3481 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
     3541 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
     3601 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
     3661 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
     3721 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
     3781 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
     3841 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
     3901 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
     3961 gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa
     4021 aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc
     4081 attaggaaat taatacgact cactata
//
