LOCUS pBIG1a_FH-nsp14 8144 bp DNA circular SYN 07-APR-2021 DEFINITION pBIG1a_3xFlag-His6-nsp14 ACCESSION pBIG1a_FH-nsp14 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 8144) AUTHORS Self JOURNAL Unpublished. FEATURES Location/Qualifiers CDS 223..1233 /label="SpecR" CDS 1859..2719 /label="AmpR" misc_signal 3783..4007 /label="Tn7R" CDS complement (4074..4607) /label="GentaR" misc_signal 5489..5519 /label="polh promoter" CDS 5627..7318 /label="3xFlag-His6-nsp14" misc_signal 7387..7578 /label="SV40 late polyA" misc_signal 7897..8062 /label="Tn7L" ORIGIN 1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga 61 ctgaatatca acgcttattt gcagcctgaa ctgggaggca gaataaatga tcatatcgtc 121 aattattacc tccacgggga gagcctgagc aaactggcct caggcatttg agaagcacac 181 ggtcacactg cttccggtag tcaataaacc ggtaagtagc gtatgcgctc acgcaactgg 241 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt 301 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac 361 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc 421 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta 481 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg 541 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg 601 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact 661 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac 721 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag 781 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc 841 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc 901 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg 961 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt 1021 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg 1081 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga 1141 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg 1201 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgg 1261 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 1321 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 1381 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 1441 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 1501 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 1561 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 1621 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 1681 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt 1741 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 1801 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 1861 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 1921 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 1981 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 2041 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 2101 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 2161 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 2221 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 2281 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 2341 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 2401 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 2461 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 2521 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 2581 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 2641 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 2701 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 2761 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 2821 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 2881 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 2941 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 3001 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 3061 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 3121 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 3181 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 3241 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 3301 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 3361 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 3421 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 3481 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 3541 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 3601 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 3661 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagacca 3721 gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg 3781 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa 3841 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc 3901 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg 3961 tcgtattaaa gaggggcgtg gccaagggca tggtaaagac tatattcgcg gcgttgtgac 4021 aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg 4081 gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa ctttgtatag 4141 agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata agcaccaagc 4201 gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg cctccggtgc 4261 tcgccggaga ctgcgagatc atagatatag atctcactac gcggctgctc aaacttgggc 4321 agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg 4381 aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg ttgggagtag 4441 gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat ggatttgact 4501 tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc taactttgtt 4561 ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt tgctgctcca 4621 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga 4681 ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct 4741 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt 4801 acgaaccgaa caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg 4861 tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga 4921 gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg 4981 caaggtgctg tgcacggatc tgcccttgct tcaggagatc ggtagacctc ggccgtcgcg 5041 gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg 5101 cgagcatcgt ttgttcgccc aggactctag ctatagttct agtggttggc tacagctttg 5161 tttgtactat caacaggttg aactgctgat caacagatcc tctacgcggc cgcggtacca 5221 taacttcgta tagcatacat tatacgaagt tatctgggtt taaacgctct atggtctaaa 5281 gtttttatcg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat 5341 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg 5401 gggttcggtc ccagggctgg cactctgtca agtgaacgct ctatggtcta aagatttaaa 5461 tcgacctact ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca 5521 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt 5581 ccggattatt cataccgtcc caccatcggg cgcggatcca gccaccatgg actacaagga 5641 ccacgacggc gattacaagg atcatgacat cgactacaaa gacgacgatg acaagggttc 5701 ccaccatcac caccatcact ctgctgtgct gcaggctgag aacgtgaccg gcctgttcaa 5761 ggactgctcc aaagtgatca ccggactgca ccccactcag gctcctactc acctgtctgt 5821 ggacaccaag ttcaagaccg agggcctgtg cgtggacatc cctggtatcc ctaaggacat 5881 gacctaccgt cgtctgatct ccatgatggg cttcaagatg aactaccaag tgaacggcta 5941 ccccaacatg ttcatcaccc gtgaagaggc tatccgtcac gtgcgcgctt ggatcggttt 6001 cgacgttgaa ggttgccacg ctaccaggga agctgtgggt actaacctgc ctctgcagct 6061 gggattctcc accggtgtta acctggtggc tgtgcccact ggttacgtgg acacccctaa 6121 caacaccgac ttctcccgtg tgtccgctaa gcctcctcct ggcgaccaat tcaagcactt 6181 gatccctctg atgtacaagg gcctgccttg gaacgtcgtg cgtatcaaga tcgtgcagat 6241 gctgtctgac accctgaaga acctgtccga ccgtgtggtg ttcgtgctgt gggctcacgg 6301 tttcgagctg acctccatga agtactttgt gaagatcggt cccgagagga cctgctgctt 6361 gtgcgataga cgtgctacct gcttctctac cgcttccgac acctacgctt gctggcacca 6421 ctccatcggc ttcgactacg tgtacaaccc cttcatgatc gacgtgcagc agtggggttt 6481 caccggcaac ctgcagtcta accacgacct gtactgccag gtgcacggca acgctcacgt 6541 ggcctcttgt gacgctatca tgacccgttg cctggctgtg cacgagtgct tcgtgaagcg 6601 tgtggactgg accatcgagt accccatcat cggcgacgag ctgaagatca acgctgcttg 6661 cagaaaggtg cagcacatgg tggtcaaggc tgctctgctg gctgacaagt tccctgtcct 6721 gcacgacatc ggcaacccca aggctattaa gtgcgtgccc caggctgacg tcgagtggaa 6781 gttctacgac gctcagccct gctccgacaa ggcttacaag atcgaggaac tgttctactc 6841 ctacgctacc cactcagaca agttcaccga cggtgtctgc ctgttctgga actgcaatgt 6901 ggatcgttac cccgctaact ccatcgtgtg ccgtttcgac acccgtgtgc tgtccaacct 6961 gaacctgcct ggttgcgacg gtggttccct gtacgtgaac aagcacgctt tccacactcc 7021 agctttcgac aagtccgctt tcgtgaacct gaagcagctg cccttcttct actactccga 7081 ctctccctgc gagtcccacg gaaagcaagt ggtgtccgac atcgattacg tgcccctgaa 7141 gtccgctacc tgcatcacca gatgcaacct cggtggtgct gtgtgccgtc accacgctaa 7201 cgagtaccgt ctgtacctgg acgcttacaa catgatgatc tccgctggtt tctccctgtg 7261 ggtgtacaag cagttcgaca cttacaacct gtggaacacc ttcaccaggc tgcagtaaaa 7321 gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag aggttttact 7381 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt 7441 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 7501 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 7561 tgtatcttat catgtctgga tctgatcact gcttgagcct agaagatccg gctgctaaca 7621 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta tcataaccat 7681 ttaaataaat ctatatctca atcggggtta cgtcgatacc ccaccgagac cccattggga 7741 ccaatacgcc cgcgtttctt ccttttcccc accccaaccc ccaagttcgg gtgaaggccc 7801 agggctcgca gccaacgtcg gggcggcaag ccctgccata gccactacgg gttaattaaa 7861 aactatatct caatcggggt ttaaacagga gatccgaacc agataagtga aatctagttc 7921 caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat 7981 ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc 8041 ccaactattt tgtccgccca cagcggggca tttttcttcc tgttatgttt ttaatcaaac 8101 atcctgccaa ctccatgtga caaaccgtca tcttcggcta cttt //