LOCUS       pBIG2ab_nsp14/10        9566 bp    DNA     circular SYN 07-APR-2021
DEFINITION  pBIG2ab_nsp14/nsp10-His6-3xFlag
ACCESSION   pBIG2ab_nsp14/10
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Self
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     misc_signal     91..1352
                     /label="CamR"
     CDS             2147..2806
                     /label="AmpR"
     misc_signal     3873..4097
                     /label="Tn7R"
     CDS             complement (4164..4697)
                     /label="GentR"
     misc_signal     5587..5617
                     /label="polh promoter"
     CDS             5725..7311
                     /label="nsp14"
     misc_signal     7380..7571
                     /label="SV40 late polyA"
     misc_signal     8080..8110
                     /label="polh promoter"
     CDS             8218..8739
                     /label="nsp10-His6-3xFlag"
     misc_signal     8808..8999
                     /label="SV40 late polyA"
     misc_signal     9319..9484
                     /label="Tn7L"
ORIGIN      
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa ctgaacagga gggacagctg atagaaacag
      121 aagccactgg agcacctcaa aaacaccatc atacactaaa tcagtaagtt ggcagcatca
      181 cccgacgcac tttgcgccga ataaatacct gtgacggaag atcacttcgc agaataaata
      241 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
      301 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
      361 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
      421 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
      481 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
      541 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
      601 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
      661 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
      721 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
      781 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
      841 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
      901 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
      961 ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt
     1021 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     1081 tattggtgcc cttaaacgcc tggtgctacg cctgaataag tgataataag cggatgaatg
     1141 gcagaaattc gaaagcaaat tcgacccggt cgtcggttca gggcagggtc gttaaatagc
     1201 cgcttatgtc tattgctggt ttaccggttt attgactacc ggaagcagtg tgaccgtgtg
     1261 cttctcaaat gcctgaggcc agtttgctca ggctctcccc gtggaggtaa taattgacga
     1321 tatgatcatt tattctgcct cccagagcct gatggcgaat gggacgcgcc ctgtagcggc
     1381 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     1441 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     1501 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     1561 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     1621 gtttttcgcc ctttgacgtt ggagtccacg ttcttaatag tggactcttg ttccaaactg
     1681 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     1741 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     1801 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     1861 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1921 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     1981 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     2041 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     2101 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     2161 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     2221 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     2281 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     2341 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     2401 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     2461 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     2521 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     2581 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     2641 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     2701 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     2761 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     2821 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     2881 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2941 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3001 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3061 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3121 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3181 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3241 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3301 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3361 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3421 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3481 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3541 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3601 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3661 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3721 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     3781 tctgtgcggt atttcacacc gcatagacca gccgcgtaac ctggcaaaat cggttacggt
     3841 tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg
     3901 aacaaaatag atctaaacta tgacaataaa gtcttaaact agacagaata gttgtaaact
     3961 gaaatcagtc cagttatgct gtgaaaaagc atactggact tttgttatgg ctaaagcaaa
     4021 ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg gccaagggca
     4081 tggtaaagac tatattcgcg gcgttgtgac aatttaccga acaactccgc ggccgggaag
     4141 ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc
     4201 acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct
     4261 gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg
     4321 agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag
     4381 atctcactac gcggctgctc aaacttgggc agaacgtaag ccgcgagagc gccaacaacc
     4441 gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg
     4501 taatcggagt ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa
     4561 agatcaagag cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg
     4621 atgcccatac ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg
     4681 ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
     4741 cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat
     4801 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
     4861 tgagcgcata cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt
     4921 tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt
     4981 cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca
     5041 ggcattggcg gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgcccttgct
     5101 tcaggagatc ggtagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga
     5161 agtggttcgc atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc aggactctag
     5221 ctatagttct agtggttggc tacagctttg tttgtactat caacaggttg aactgctgat
     5281 caacagatcc tctacgcggc cgcggtacca taacttcgta tagcatacat tatacgaagt
     5341 tatctggcct aggttaatta aacgctctat ggtctaaagt ttttatcgag gctaactgaa
     5401 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     5461 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     5521 ctctgtcaag tgaacgctct atggtctaaa gatttaaatc gacctactcc ggaatattaa
     5581 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     5641 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     5701 ccatcgggcg cggatccagc caccatggct gagaacgtga ccggcctgtt caaggactgc
     5761 tccaaagtga tcaccggact gcaccccact caggctccta ctcacctgtc tgtggacacc
     5821 aagttcaaga ccgagggcct gtgcgtggac atccctggta tccctaagga catgacctac
     5881 cgtcgtctga tctccatgat gggcttcaag atgaactacc aagtgaacgg ctaccccaac
     5941 atgttcatca cccgtgaaga ggctatccgt cacgtgcgcg cttggatcgg tttcgacgtt
     6001 gaaggttgcc acgctaccag ggaagctgtg ggtactaacc tgcctctgca gctgggattc
     6061 tccaccggtg ttaacctggt ggctgtgccc actggttacg tggacacccc taacaacacc
     6121 gacttctccc gtgtgtccgc taagcctcct cctggcgacc aattcaagca cttgatccct
     6181 ctgatgtaca agggcctgcc ttggaacgtc gtgcgtatca agatcgtgca gatgctgtct
     6241 gacaccctga agaacctgtc cgaccgtgtg gtgttcgtgc tgtgggctca cggtttcgag
     6301 ctgacctcca tgaagtactt tgtgaagatc ggtcccgaga ggacctgctg cttgtgcgat
     6361 agacgtgcta cctgcttctc taccgcttcc gacacctacg cttgctggca ccactccatc
     6421 ggcttcgact acgtgtacaa ccccttcatg atcgacgtgc agcagtgggg tttcaccggc
     6481 aacctgcagt ctaaccacga cctgtactgc caggtgcacg gcaacgctca cgtggcctct
     6541 tgtgacgcta tcatgacccg ttgcctggct gtgcacgagt gcttcgtgaa gcgtgtggac
     6601 tggaccatcg agtaccccat catcggcgac gagctgaaga tcaacgctgc ttgcagaaag
     6661 gtgcagcaca tggtggtcaa ggctgctctg ctggctgaca agttccctgt cctgcacgac
     6721 atcggcaacc ccaaggctat taagtgcgtg ccccaggctg acgtcgagtg gaagttctac
     6781 gacgctcagc cctgctccga caaggcttac aagatcgagg aactgttcta ctcctacgct
     6841 acccactcag acaagttcac cgacggtgtc tgcctgttct ggaactgcaa tgtggatcgt
     6901 taccccgcta actccatcgt gtgccgtttc gacacccgtg tgctgtccaa cctgaacctg
     6961 cctggttgcg acggtggttc cctgtacgtg aacaagcacg ctttccacac tccagctttc
     7021 gacaagtccg ctttcgtgaa cctgaagcag ctgcccttct tctactactc cgactctccc
     7081 tgcgagtccc acggaaagca agtggtgtcc gacatcgatt acgtgcccct gaagtccgct
     7141 acctgcatca ccagatgcaa cctcggtggt gctgtgtgcc gtcaccacgc taacgagtac
     7201 cgtctgtacc tggacgctta caacatgatg atctccgctg gtttctccct gtgggtgtac
     7261 aagcagttcg acacttacaa cctgtggaac accttcacca ggctgcagta aaagcttgtc
     7321 gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     7381 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     7441 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7501 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7561 tatcatgtct ggatctgatc actgcttgag cctagaagat ccggctgcta acaaagcccg
     7621 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctatcataac catttaaata
     7681 aatctatatc tcaatcgggg ttacgtcgat accccaccga gaccccattg ggaccaatac
     7741 gcccgcgttt cttccttttc cccaccccaa cccccaagtt cgggtgaagg cccagggctc
     7801 gcagccaacg tcggggcggc aagccctgcc atagccacta cgggttaatt aaaaactata
     7861 tctcaatcgg ggtttttatc gaggctaact gaaacacgga aggagacaat accggaagga
     7921 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
     7981 tcataaacgc ggggttcggt cccagggctg gcactctgtc aagtgaacgc tctatggtct
     8041 aaagatttaa atcgacctac tccggaatat taatagatca tggagataat taaaatgata
     8101 accatctcgc aaataaataa gtattttact gttttcgtaa cagttttgta ataaaaaaac
     8161 ctataaatat tccggattat tcataccgtc ccaccatcgg gcgcggatcc agccaccatg
     8221 gctggaaacg ctaccgaggt gcccgctaac tctaccgtgc tgtccttctg cgctttcgct
     8281 gtggacgctg ctaaggctta caaggactac ctggcttccg gtggccagcc tatcaccaac
     8341 tgcgtgaaga tgctgtgcac ccacaccggt actggacagg ctatcactgt gacccctgag
     8401 gctaacatgg accaagagtc cttcggtggt gcttcctgct gcctgtactg ccgttgccac
     8461 atcgatcacc ccaatcctaa gggcttctgc gacctgaagg gcaaatacgt gcagatcccc
     8521 accacttgcg ctaacgaccc tgtgggtttc accctgaaga acaccgtgtg caccgtctgc
     8581 ggcatgtgga aaggatacgg ttgctcctgc gaccagctgc gcgaacccat gttgcagggt
     8641 ggctcccacc atcaccacca tcacggttct gactacaagg accacgacgg cgattacaag
     8701 gatcatgaca tcgactacaa agacgacgat gacaagtaaa agcttgtcga gaagtactag
     8761 aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     8821 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     8881 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8941 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     9001 atctgatcac tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa aggaagctga
     9061 gttggctgct gccaccgctg agcaataact atcataacca tttaaataaa tctatatctc
     9121 aatcggggtt acgtcgatac cccaccgaga ccccattggg accaatacgc ccgcgtttct
     9181 tccttttccc caccccaacc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     9241 ggggcggcaa gccctgccat agccactacg ggttaattaa aaactggata ctattgcacg
     9301 tttcaatcag gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt
     9361 ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc
     9421 ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc
     9481 cacagcgggg catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt
     9541 gacaaaccgt catcttcggc tacttt
//
