LOCUS       nsp12-HF/7/8           12119 bp    DNA     circular SYN 25-MAR-2021
DEFINITION  pBIG2abc_nsp12-HF/7/8
ACCESSION   nsp12-HF/7/8
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 12119)
  AUTHORS   Self
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     misc_signal     91..1352
                     /label="CamR"
     CDS             2147..2806
                     /label="AmpR"
     misc_signal     3873..4097
                     /label="Tn7R"
     CDS             complement (4164..4697)
                     /label="GentR"
     misc_signal     5587..5617
                     /label="polh promoter"
     CDS             5725..8625
                     /label="nsp12-His6-3xFlag"
     misc_signal     8694..8885
                     /label="SV40 late polyA"
     misc_signal     9394..9424
                     /label="polh promoter"
     CDS             9532..9786
                     /label="nsp7"
     misc_signal     9855..10046
                     /label="SV40 late polyA"
     misc_signal     10555..10585
                     /label="polh promoter"
     CDS             10693..11292
                     /label="nsp8"
     misc_signal     11361..11552
                     /label="SV40 late polyA"
     misc_signal     11872..12037
                     /label="Tn7L"
ORIGIN      
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa ctgaacagga gggacagctg atagaaacag
      121 aagccactgg agcacctcaa aaacaccatc atacactaaa tcagtaagtt ggcagcatca
      181 cccgacgcac tttgcgccga ataaatacct gtgacggaag atcacttcgc agaataaata
      241 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
      301 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
      361 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
      421 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
      481 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
      541 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
      601 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
      661 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
      721 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
      781 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
      841 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
      901 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
      961 ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt
     1021 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     1081 tattggtgcc cttaaacgcc tggtgctacg cctgaataag tgataataag cggatgaatg
     1141 gcagaaattc gaaagcaaat tcgacccggt cgtcggttca gggcagggtc gttaaatagc
     1201 cgcttatgtc tattgctggt ttaccggttt attgactacc ggaagcagtg tgaccgtgtg
     1261 cttctcaaat gcctgaggcc agtttgctca ggctctcccc gtggaggtaa taattgacga
     1321 tatgatcatt tattctgcct cccagagcct gatggcgaat gggacgcgcc ctgtagcggc
     1381 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     1441 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     1501 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     1561 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     1621 gtttttcgcc ctttgacgtt ggagtccacg ttcttaatag tggactcttg ttccaaactg
     1681 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     1741 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     1801 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     1861 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1921 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     1981 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     2041 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     2101 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     2161 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     2221 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     2281 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     2341 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     2401 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     2461 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     2521 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     2581 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     2641 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     2701 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     2761 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     2821 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     2881 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2941 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3001 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3061 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3121 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3181 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3241 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3301 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3361 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3421 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3481 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3541 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3601 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3661 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3721 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     3781 tctgtgcggt atttcacacc gcatagacca gccgcgtaac ctggcaaaat cggttacggt
     3841 tgagtaataa atggatgccc tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg
     3901 aacaaaatag atctaaacta tgacaataaa gtcttaaact agacagaata gttgtaaact
     3961 gaaatcagtc cagttatgct gtgaaaaagc atactggact tttgttatgg ctaaagcaaa
     4021 ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg gccaagggca
     4081 tggtaaagac tatattcgcg gcgttgtgac aatttaccga acaactccgc ggccgggaag
     4141 ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc
     4201 acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct
     4261 gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg
     4321 agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag
     4381 atctcactac gcggctgctc aaacttgggc agaacgtaag ccgcgagagc gccaacaacc
     4441 gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg
     4501 taatcggagt ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa
     4561 agatcaagag cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg
     4621 atgcccatac ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg
     4681 ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
     4741 cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat
     4801 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
     4861 tgagcgcata cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt
     4921 tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt
     4981 cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca
     5041 ggcattggcg gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgcccttgct
     5101 tcaggagatc ggtagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga
     5161 agtggttcgc atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc aggactctag
     5221 ctatagttct agtggttggc tacagctttg tttgtactat caacaggttg aactgctgat
     5281 caacagatcc tctacgcggc cgcggtacca taacttcgta tagcatacat tatacgaagt
     5341 tatctggcct aggttaatta aacgctctat ggtctaaagt ttttatcgag gctaactgaa
     5401 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     5461 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     5521 ctctgtcaag tgaacgctct atggtctaaa gatttaaatc gacctactcc ggaatattaa
     5581 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     5641 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     5701 ccatcgggcg cggatccagc caccatgtct gctgacgctc agtccttcct gaaccgtgtg
     5761 tgcggtgtct ctgctgctcg tctgactcct tgcggtactg gaacctctac cgacgtggtg
     5821 taccgtgctt tcgacatcta caacgacaag gtggccggtt tcgctaagtt cctcaagacc
     5881 aactgctgcc gcttccaaga gaaggacgag gacgacaacc tgatcgactc ctacttcgtg
     5941 gtcaagcgtc acaccttctc caactaccag cacgaggaaa ctatctacaa cctgctgaag
     6001 gactgccccg ctgtggctaa gcacgacttc ttcaagttcc gtatcgacgg cgacatggtg
     6061 ccccacatct ctcgtcagcg tctgaccaag tacaccatgg ctgacctggt gtacgctctg
     6121 cgccacttcg atgagggaaa ctgcgacacc ctgaaagaga tcctggtcac ctacaactgc
     6181 tgtgacgacg actacttcaa caagaaggat tggtacgact tcgtcgagaa ccccgacatc
     6241 ctgcgtgtgt acgctaacct gggagagcgt gtgcgtcagg ctctgctcaa gactgtgcag
     6301 ttctgcgacg ctatgcgtaa cgctggtatc gtgggagtgc tgaccctgga caaccaggac
     6361 ctgaacggca attggtatga tttcggcgac ttcatccaga ctacccctgg ttctggtgtc
     6421 cccgtggtgg actcttacta ctccctgctg atgcccatcc tgactctgac ccgtgctctg
     6481 accgctgagt ctcacgtgga caccgacctg accaagcctt acatcaagtg ggacttgctg
     6541 aagtacgatt tcaccgagga acgcctgaag ctgttcgacc gttacttcaa gtactgggac
     6601 cagacctacc atcctaactg cgtgaactgc ctggacgacc gttgcatcct gcactgcgct
     6661 aacttcaacg tgctgttctc cactgtgttc cctccaacct ccttcggtcc tctcgtgcgc
     6721 aagatcttcg tggacggtgt ccctttcgtg gtgtccaccg gttaccactt ccgtgaactg
     6781 ggtgtcgtgc ataaccagga cgtcaacctc cactcctctc gtctgtcctt caaagagctg
     6841 ctggtctacg ctgctgaccc cgctatgcat gctgcttccg gaaacctgct gctggacaag
     6901 cgtaccactt gcttctccgt ggctgccctg actaacaacg tggccttcca gaccgtgaag
     6961 cccggcaatt tcaacaagga cttctacgat ttcgccgtgt ccaagggctt cttcaaagaa
     7021 ggctcctccg tcgagctgaa gcacttcttc ttcgctcaag acggcaacgc tgctatctcc
     7081 gactacgact actaccgtta caacttgccc accatgtgcg acatccgtca gctgctgttc
     7141 gtggtcgagg tggtggacaa gtacttcgac tgctacgacg gtggttgcat caacgccaat
     7201 caagtgatcg tgaacaacct ggacaagtcc gctggcttcc ccttcaacaa atggggcaaa
     7261 gctcgtctgt actacgactc catgtcctac gaggaccagg acgctctgtt cgcttacacc
     7321 aagcgtaacg tgatccccac catcactcag atgaacctga aatacgccat ctccgctaag
     7381 aaccgtgctc gtaccgtggc tggtgtctcc atctgctcca ccatgaccaa ccgtcagttc
     7441 caccagaagc tgctgaagtc aatcgctgct acccgcggtg ctaccgtggt catcggtact
     7501 tccaagttct acggtggctg gcacaacatg ctgaaaaccg tgtactccga cgtggaaaac
     7561 cctcacctga tgggctggga ctaccccaag tgcgatcgtg ctatgcctaa catgctgcgt
     7621 atcatggctt ccctggtgct ggctcgcaag cacactactt gctgctccct gtctcaccgt
     7681 ttctaccgtc tggctaacga gtgcgctcag gtgctgtccg agatggtcat gtgcggtggt
     7741 tccctgtacg tgaagcctgg tggtacttcc tctggcgacg ctaccaccgc ttacgctaac
     7801 tccgtgttca acatctgcca ggctgtgacc gctaacgtga acgctctgct gtccaccgac
     7861 ggcaacaaga tcgctgacaa atacgtgcgt aacctgcagc accgtctgta cgagtgcctg
     7921 taccgtaacc gtgacgtcga cactgacttc gtgaacgagt tctacgctta cctgcgcaag
     7981 cacttcagca tgatgatcct gtccgacgac gctgtcgtgt gcttcaactc cacctacgct
     8041 tcccaaggcc tggtggcttc catcaagaac ttcaagtccg tgctgtacta ccagaacaac
     8101 gtgttcatgt ccgaggctaa gtgctggacc gagactgacc tgactaaggg tccccacgag
     8161 ttctgctccc agcacaccat gctggtcaag cagggcgacg attacgtgta cctgccttat
     8221 cctgatcctt ctcgtatcct cggcgctggt tgcttcgtgg atgacatcgt caagaccgac
     8281 ggaaccctga tgatcgagcg tttcgtgtcc ctggctatcg acgcttaccc tctgactaag
     8341 caccccaatc aagagtacgc tgacgtgttc cacctgtacc tgcagtacat ccgcaagctg
     8401 cacgacgagc tgaccggaca catgctggac atgtactccg tgatgctgac caacgacaac
     8461 acctctcgtt actgggagcc cgaattctac gaggctatgt acacccctca caccgtgctg
     8521 cagggtggct cccaccatca ccaccatcac ggttctgact acaaggacca cgacggcgat
     8581 tacaaggatc atgacatcga ctacaaagac gacgatgaca agtaaaagct tgtcgagaag
     8641 tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     8701 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     8761 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8821 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8881 gtctggatct gatcactgct tgagcctaga agatccggct gctaacaaag cccgaaagga
     8941 agctgagttg gctgctgcca ccgctgagca ataactatca taaccattta aataaatcta
     9001 tatctcaatc ggggttacgt cgatacccca ccgagacccc attgggacca atacgcccgc
     9061 gtttcttcct tttccccacc ccaaccccca agttcgggtg aaggcccagg gctcgcagcc
     9121 aacgtcgggg cggcaagccc tgccatagcc actacgggtt aattaaaaac tatatctcaa
     9181 tcggggtttt tatcgaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     9241 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa
     9301 acgcggggtt cggtcccagg gctggcactc tgtcaagtga acgctctatg gtctaaagat
     9361 ttaaatcgac ctactccgga atattaatag atcatggaga taattaaaat gataaccatc
     9421 tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa
     9481 atattccgga ttattcatac cgtcccacca tcgggcgcgg atccagccac catgtccaag
     9541 atgtccgacg tgaagtgcac ctccgtggtg ctgctgtctg tgctgcaaca gctgcgtgtg
     9601 gaatcctcca gcaagctgtg ggctcagtgc gtccaactgc acaacgacat cctgctggct
     9661 aaggacacca ccgaggcttt cgagaagatg gtgtccctgc tgtccgtcct gctgtctatg
     9721 caaggtgctg tggacatcaa caagctgtgc gaagagatgc tggacaaccg tgctaccctg
     9781 cagtaaaagc ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag
     9841 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     9901 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9961 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10021 ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag aagatccggc
    10081 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactatc
    10141 ataaccattt aaataaatct atatctcaat cggggttacg tcgatacccc accgagaccc
    10201 cattgggacc aatacgcccg cgtttcttcc ttttccccac cccaaccccc aagttcgggt
    10261 gaaggcccag ggctcgcagc caacgtcggg gcggcaagcc ctgccatagc cactacgggt
    10321 taattaaaaa ctggatacta ttgcacgttt ttatcgaggc taactgaaac acggaaggag
    10381 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg
    10441 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcaagtg
    10501 aacgctctat ggtctaaaga tttaaatcga cctactccgg aatattaata gatcatggag
    10561 ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt
    10621 ttgtaataaa aaaacctata aatattctgg attattcata ccgtcccacc atcgggcgcg
    10681 gatccagcca ccatggctat cgcttccgag ttctcctctc tgccctccta cgctgctttc
    10741 gctaccgctc aagaggctta cgagcaggct gtggctaacg gcgactctga ggtggtgctg
    10801 aagaagctga aaaagtccct gaacgtggcc aagtccgagt tcgaccgtga cgctgctatg
    10861 cagcgcaagc tcgagaagat ggctgaccag gctatgaccc agatgtacaa gcaggctcgt
    10921 tccgaggaca agcgtgctaa agtgacctcc gctatgcaga ccatgctgtt caccatgctc
    10981 cgcaagctgg acaacgacgc tctgaacaac atcatcaaca acgctcgtga cggttgcgtg
    11041 cccctgaaca tcatccctct gaccaccgct gctaagctga tggtggtcat ccccgactac
    11101 aacacctaca agaacacttg cgacggcacc accttcacct acgcttccgc tctgtgggag
    11161 atccagcaag tggtggacgc tgactccaag atcgtgcagc tgtccgagat ctccatggac
    11221 aactctccca acctggcttg gcccctgatc gtgactgctc tgcgtgctaa ctccgctgtg
    11281 aagctgcagt aaaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt
    11341 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
    11401 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
    11461 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    11521 tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga gcctagaaga
    11581 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
    11641 actatcataa ccatttaaat aaatctatat ctcaatcggg gttacgtcga taccccaccg
    11701 agaccccatt gggaccaata cgcccgcgtt tcttcctttt ccccacccca acccccaagt
    11761 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caagccctgc catagccact
    11821 acgggttaat taaaaaccta atgatgcctg atgtttcaat caggagatcc gaaccagata
    11881 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
    11941 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc
    12001 cacccctccc agttcccaac tattttgtcc gcccacagcg gggcattttt cttcctgtta
    12061 tgtttttaat caaacatcct gccaactcca tgtgacaaac cgtcatcttc ggctacttt
//
