LOCUS       14His-SUMO-nsp7         4540 bp    DNA     circular SYN 08-APR-2021
DEFINITION  pK27Sumo_14His-SUMO-nsp7
ACCESSION   14His-SUMO-nsp7
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Self
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     misc_signal     10..54
                     /label="T5 promoter"
     CDS             115..735
                     /label="14His-SUMO-nsp7"
     CDS             193..483
                     /label="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
     CDS             937..1752
                     /label="KanR"
                     /product="aminoglycoside phosphotransferase"
     CDS             complement (2151..3233)
                     /label="lacI"
                     /product="lac repressor"
ORIGIN      
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatgagc
      121 aagcatcacc atcattcagg ccatcaccat accggacacc accatcattc aggcagtcat
      181 caccataccg gatcggactc agaagtcaat caagaagcta agccagaggt caagccagaa
      241 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc
      301 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa aagacagggt
      361 aaggaaatgg actccttaag attcttgtac gacggtatta gaattcaagc tgatcagacc
      421 cctgaagatt tggacatgga ggataacgat attattgagg ctcacagaga acagattggt
      481 ggcagcaaaa tgtccgatgt taaatgcacc agcgttgttc tgctgagcgt tctgcagcag
      541 ctgcgtgttg aaagcagcag caaactgtgg gcacagtgtg ttcagctgca taatgatatt
      601 ctgctggcaa aagataccac cgaagccttt gaaaaaatgg ttagcctgct gagtgtgctg
      661 ctgagcatgc agggtgcagt tgatattaac aaactgtgtg aagagatgct ggataatcgt
      721 gcaaccctgc agtaataagc ggccgcacat caccatcact aagcttaatt agctgagctt
      781 ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg
      841 ctcggttgcc gccgggcgtt ttttattggt gagaatccaa gctagccatg aacaataaaa
      901 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
      961 tcttgctcta ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     1021 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat
     1081 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     1141 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     1201 cgtactcctg atgatgcttg gttactcacg actgcgatcc ccggcaaaac agcattccag
     1261 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     1321 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     1381 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     1441 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcacaaact tttgccattc
     1501 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     1561 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     1621 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     1681 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     1741 gagtttttct aagaattaat tcatgggcaa atattatacg caaggcgaca aggtgctgat
     1801 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
     1861 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag
     1921 ttattggtgc ccttaaacgc ctggggtaat gactctctag cttgaggcat caaataaaac
     1981 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2041 tcctgagtag gacaaatccg ccgctctaga ttacgtgcag tcgatgataa gctgtcaaac
     2101 atgagaattg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg
     2161 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2221 gaggcggttt gcgtattggg ggccagggtg gtttttcttt tcaccagtga gacgggcaac
     2281 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
     2341 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg
     2401 tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg
     2461 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
     2521 acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg
     2581 ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc
     2641 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
     2701 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata
     2761 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
     2821 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
     2881 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
     2941 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt
     3001 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg
     3061 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
     3121 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc
     3181 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
     3241 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcaccat
     3301 tcgatggtgt cggaatttcg ggcagcgttg ggtcctggcc acgggtgcgc atgatctaga
     3361 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     3421 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     3481 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     3541 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     3601 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     3661 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3721 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3781 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3841 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3901 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3961 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4021 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4081 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4141 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4201 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4261 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4321 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4381 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4441 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgacattaac
     4501 ctataaaaat aggcgtatca cgaggccctt tcgtcttcac
//
