LOCUS 14His-SUMO-nsp8 4885 bp DNA circular SYN 23-MAR-2021 DEFINITION pK27Sumo_14His-SUMO-nsp8 ACCESSION 14His-SUMO-nsp8 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4885) AUTHORS Self JOURNAL Unpublished. FEATURES Location/Qualifiers misc_signal 10..54 /label="T5 promoter" CDS 115..1080 /label="14His-SUMO-nsp8" CDS 193..483 /label="S. cerevisiae SMT3 (truncated)" /product="cleavable ubiquitin-like protein tag" CDS 1282..2097 /label="KanR" /product="aminoglycoside phosphotransferase" CDS complement (2496..3578) /label="lacI" /product="lac repressor" ORIGIN 1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca 61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatgagc 121 aagcatcacc atcattcagg ccatcaccat accggacacc accatcattc aggcagtcat 181 caccataccg gatcggactc agaagtcaat caagaagcta agccagaggt caagccagaa 241 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc 301 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa aagacagggt 361 aaggaaatgg actccttaag attcttgtac gacggtatta gaattcaagc tgatcagacc 421 cctgaagatt tggacatgga ggataacgat attattgagg ctcacagaga acagattggt 481 ggcgcaattg ccagcgaatt tagcagcctg ccgagctatg cagcatttgc aaccgcacaa 541 gaagcatacg aacaggcagt tgccaatggt gatagcgaag ttgttctgaa aaaactgaaa 601 aagtcactga acgtggccaa aagcgaattt gatcgtgatg cagcaatgca gcgtaaactg 661 gaaaagatgg cagatcaggc aatgacccag atgtataaac aggcacgtag cgaagataaa 721 cgtgcaaaag ttaccagcgc aatgcagacc atgctgttta ccatgctgcg caaactggat 781 aatgatgccc tgaacaacat tatcaataat gcccgtgatg gttgtgttcc gctgaacatt 841 attccgctga ccaccgcagc aaaactgatg gtggttattc cggattacaa cacctataaa 901 aacacctgtg atggcaccac ctttacctat gcaagcgcac tgtgggaaat tcagcaggtt 961 gttgatgcag atagcaaaat tgtgcagctg agcgaaatta gcatggataa tagcccgaat 1021 ctggcatggc ctctgattgt taccgcactg cgtgcaaata gcgcagttaa actgcagtaa 1081 taagcggccg cacatcacca tcactaagct taattagctg agcttggact cctgttgata 1141 gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg 1201 gcgtttttta ttggtgagaa tccaagctag ccatgaacaa taaaactgtc tgcttacata 1261 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg ctctaggccg 1321 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc 1381 gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt 1441 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac 1501 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat 1561 gcttggttac tcacgactgc gatccccggc aaaacagcat tccaggtatt agaagaatat 1621 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg 1681 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa 1741 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg 1801 cctgttgaac aagtctggaa agaaatgcac aaacttttgc cattctcacc ggattcagtc 1861 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt 1921 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg 1981 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt 2041 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaagaa 2101 ttaattcatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca 2161 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca 2221 gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta 2281 aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag gctcagtcga 2341 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa 2401 atccgccgct ctagattacg tgcagtcgat gataagctgt caaacatgag aattgtgcct 2461 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 2521 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 2581 ttgggggcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc 2641 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga 2701 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg 2761 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt 2821 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc 2881 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct 2941 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc 3001 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga 3061 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc 3121 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg 3181 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga 3241 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg 3301 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc 3361 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt 3421 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt 3481 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg 3541 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc 3601 tcttccgggc gctatcatgc cataccgcga aaggttttgc accattcgat ggtgtcggaa 3661 tttcgggcag cgttgggtcc tggccacggg tgcgcatgat ctagagctgc ctcgcgcgtt 3721 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 3781 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 3841 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 3901 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 3961 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 4021 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 4081 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 4141 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 4201 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 4261 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 4321 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 4381 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 4441 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 4501 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 4561 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 4621 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 4681 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 4741 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 4801 gtggaacgaa aactcacgtt aagggatttt ggtcatgaca ttaacctata aaaataggcg 4861 tatcacgagg ccctttcgtc ttcac //