LOCUS       14His-SUMO-nsp12        7087 bp    DNA     circular SYN 23-MAR-2021
DEFINITION  pK27Sumo_14His-SUMO-nsp12
ACCESSION   14His-SUMO-nsp12
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Self
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     misc_signal     10..54
                     /label="T5 promoter"
     CDS             115..3282
                     /label="14His-SUMO-nsp12"
     CDS             193..483
                     /label="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
     CDS             3484..4299
                     /label="KanR"
                     /product="aminoglycoside phosphotransferase"
     CDS             complement (4698..5780)
                     /label="lacI"
                     /product="lac repressor"
ORIGIN      
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatgagc
      121 aagcatcacc atcattcagg ccatcaccat accggacacc accatcattc aggcagtcat
      181 caccataccg gatcggactc agaagtcaat caagaagcta agccagaggt caagccagaa
      241 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc
      301 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa aagacagggt
      361 aaggaaatgg actccttaag attcttgtac gacggtatta gaattcaagc tgatcagacc
      421 cctgaagatt tggacatgga ggataacgat attattgagg ctcacagaga acagattggt
      481 ggcagcgcag atgcacagag ctttctgaat cgtgtttgtg gtgttagcgc agcacgtctg
      541 accccgtgtg gcaccggcac cagcaccgat gttgtttatc gtgcatttga tatctacaac
      601 gataaagttg ccggtttcgc caaatttctg aaaaccaatt gttgccgctt ccaagagaaa
      661 gatgaagatg ataatctgat cgacagctac tttgtggtta aacgtcatac ctttagcaac
      721 tatcagcacg aggaaaccat ttacaatctg ctgaaagatt gtccggcagt tgccaaacat
      781 gattttttca aatttcgcat cgatggcgat atggttccgc atattagccg tcagcgtctg
      841 accaaatata ccatggcaga tctggtttat gcactgcgtc attttgatga aggtaattgc
      901 gataccctga aagaaattct ggtgacctat aattgttgcg acgatgacta cttcaacaag
      961 aaagattggt atgatttcgt ggaaaacccg gatattctgc gtgtttatgc aaatctgggt
     1021 gaacgtgttc gtcaggcact gctgaaaacc gttcagtttt gtgatgcaat gcgtaatgca
     1081 ggtattgttg gtgttctgac cctggataat caggatctga atggtaattg gtacgatttt
     1141 ggcgatttta ttcagaccac acctggtagc ggtgttccgg ttgttgatag ctattatagc
     1201 ctgctgatgc cgattctgac actgacccgt gcactgaccg cagaaagcca tgttgatacc
     1261 gatctgacaa aaccgtacat taaatgggac ctgctgaaat atgattttac cgaagaacgc
     1321 ctgaaactgt tcgatcgcta tttcaaatat tgggaccaga cctatcatcc gaattgtgtt
     1381 aattgtctgg atgatcgttg tattctgcat tgcgccaatt ttaacgttct gtttagcacc
     1441 gtttttccgc ctaccagctt tggtccgctg gttcgtaaaa tctttgttga tggtgtgccg
     1501 tttgttgtta gcaccggtta tcattttcgt gaactgggtg ttgttcataa ccaggatgtt
     1561 aatctgcata gcagccgtct gagctttaaa gaactgctgg tgtatgcagc agatccggca
     1621 atgcatgcag caagcggtaa tctgctgctg gataaacgta ccacctgttt tagcgttgca
     1681 gcactgacca ataatgttgc atttcagacc gttaaaccgg gtaatttcaa caaggatttc
     1741 tatgattttg ccgtgagcaa gggctttttc aaagaaggta gcagcgttga actgaagcat
     1801 tttttctttg cacaggatgg taatgcagcc atcagcgatt atgattatta tcgctataat
     1861 ctgccgacca tgtgtgatat tcgtcagctg ctgtttgttg tggaagttgt ggataaatac
     1921 ttcgattgct atgatggcgg ttgcattaac gcaaatcagg tgattgttaa taacctggac
     1981 aaaagcgcag gtttcccgtt taacaaatgg ggtaaagccc gtctgtatta tgatagcatg
     2041 agctatgaag atcaggatgc actgtttgca tataccaaac gtaatgtgat tccgaccatt
     2101 acgcagatga atctgaaata cgcaattagc gcaaaaaatc gtgcacgtac cgttgccggt
     2161 gttagcattt gtagcaccat gaccaatcgt cagtttcatc agaaactgct gaagagtatt
     2221 gcagcaaccc gtggtgcaac cgttgttatt ggtacaagca aattctatgg tggctggcat
     2281 aatatgctta agaccgtgta ttcagatgtg gaaaatccgc atctgatggg ttgggattat
     2341 ccgaaatgtg atcgtgcaat gccgaatatg ctgcgtatta tggcaagcct ggttctggca
     2401 cgtaaacata ccacatgttg cagcctgagc catcgttttt atcgtctggc aaatgaatgt
     2461 gcacaggttc tgagcgaaat ggttatgtgt ggtggtagcc tgtatgttaa acctggtggc
     2521 accagctcag gtgatgcaac caccgcctat gcaaatagcg tttttaacat ttgtcaggcc
     2581 gttaccgcca atgttaatgc cctgctgagt accgatggca acaaaattgc agataaatat
     2641 gtgcgcaatc tgcagcatcg tctgtatgaa tgtctgtatc gtaatcgtga tgtggatacc
     2701 gactttgtga atgagtttta tgcctatctg cgcaaacact tcagcatgat gattctgagt
     2761 gatgatgccg ttgtttgctt taatagcacc tatgcaagcc aaggtctggt tgcaagcatc
     2821 aaaaacttta aaagcgtgct gtactaccag aacaatgtgt ttatgagcga agcaaaatgt
     2881 tggaccgaaa ccgacctgac caaaggtccg catgaatttt gtagccagca taccatgctg
     2941 gttaaacagg gtgatgatta tgtttatctg ccgtatcctg atccgagccg tattttaggt
     3001 gcaggttgtt ttgtggatga catcgttaaa accgatggta cgctgatgat tgaacgtttt
     3061 gttagcctgg caattgatgc atatccgctg accaaacatc cgaatcaaga atatgcagat
     3121 gtgttccatc tgtacctgca gtatattcgt aaactgcatg atgaactgac aggtcacatg
     3181 ctggatatgt atagcgttat gctgaccaat gataatacca gccgttattg ggaacccgaa
     3241 ttttatgaag caatgtatac accgcatacc gtgctgcagt aataagcggc cgcacatcac
     3301 catcactaag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
     3361 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
     3421 aatccaagct agccatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     3481 gttatgagcc atattcaacg ggaaacgtct tgctctaggc cgcgattaaa ttccaacatg
     3541 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     3601 atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     3661 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     3721 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcttggtt actcacgact
     3781 gcgatccccg gcaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     3841 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     3901 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     3961 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     4021 aaagaaatgc acaaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     4081 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     4141 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     4201 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     4261 aaattgcagt ttcatttgat gctcgatgag tttttctaag aattaattca tgggcaaata
     4321 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
     4381 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     4441 gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg gggtaatgac
     4501 tctctagctt gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     4561 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ctctagatta
     4621 cgtgcagtcg atgataagct gtcaaacatg agaattgtgc ctaatgagtg agctaactta
     4681 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     4741 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggggc cagggtggtt
     4801 tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag
     4861 agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg
     4921 gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata
     4981 tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga
     5041 tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt
     5101 tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg
     5161 cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg
     5221 cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc
     5281 gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga
     5341 aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc
     5401 ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta
     5461 caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg
     5521 gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg
     5581 gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg
     5641 taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg
     5701 gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg
     5761 tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat
     5821 gccataccgc gaaaggtttt gcaccattcg atggtgtcgg aatttcgggc agcgttgggt
     5881 cctggccacg ggtgcgcatg atctagagct gcctcgcgcg tttcggtgat gacggtgaaa
     5941 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     6001 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     6061 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     6121 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     6181 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6781 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     6841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7021 ttaagggatt ttggtcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     7081 tcttcac
//
