LOCUS       pLIB_FH-nsp13           6804 bp    DNA     circular SYN 08-APR-2021
DEFINITION  pLIB_3xFlag-His6-nsp13
ACCESSION   pLIB_FH-nsp13
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Self
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     CDS             687..1547
                     /label="AmpR"
     misc_signal     2611..2835
                     /label="Tn7R"
     CDS             complement (2902..3435)
                     /label="GentaR"
     misc_signal     4132..4162
                     /label="polh promoter"
     CDS             4270..6183
                     /label="3xFlag-His6-nsp13"
     misc_signal     6252..6443
                     /label="SV40 late polyA"
     misc_signal     6557..6722
                     /label="Tn7L"
ORIGIN      
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cagctttgtt tgtactatca acaggttgaa ctgctgatca
     4021 acagatcctc tacgcggccg cggtaccata acttcgtata gcatacatta tacgaagtta
     4081 tctggtttta ctagtatcga ttcgcgacct actccggaat attaatagat catggagata
     4141 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
     4201 taataaaaaa acctataaat attccggatt attcataccg tcccaccatc gggcgcggat
     4261 ccagccacca tggactacaa ggaccacgac ggcgattaca aggatcatga catcgactac
     4321 aaagacgacg atgacaaggg ttcccaccat caccaccatc actctgctgt gctgcaggct
     4381 gttggtgctt gcgtgctgtg caactcccag acctctttgc gttgcggtgc ttgtatccgt
     4441 cgtccattcc tgtgctgcaa gtgctgctac gaccacgtga tctccacctc tcacaagctg
     4501 gtgctgtctg tgaaccccta cgtgtgcaac gctcctggtt gcgacgtgac cgatgtgacc
     4561 caactgtacc tcggtggcat gtcctactac tgcaagtccc acaagcctcc aatcagcttc
     4621 cctctgtgcg ctaacggaca ggtgttcggc ctgtacaaga acacttgcgt gggttccgac
     4681 aacgtcaccg acttcaacgc tatcgctacc tgcgactgga ccaacgctgg cgactacatc
     4741 ctggctaaca cttgcaccga gcgtctgaag ctgttcgctg ctgagactct gaaggctacc
     4801 gaggaaacct tcaagctgtc ctacggtatc gccaccgtgc gtgaagtgct gtccgaccgt
     4861 gaactgcacc tgtcctggga agtgggaaag cctcgtcctc ctctgaaccg taactacgtg
     4921 ttcaccggtt accgtgtgac caagaactcc aaggtgcaga tcggcgagta caccttcgag
     4981 aagggcgatt acggcgacgc tgtggtgtac cgtggtacta ccacctacaa gctgaacgtg
     5041 ggcgactact tcgtgctgac ttcccacacc gtgatgcccc tgtctgctcc tactctggtg
     5101 ccccaagagc actacgtgcg tatcaccgga ctgtacccca ctctgaatat ctccgacgag
     5161 ttctcctcca acgtggccaa ctaccagaaa gtgggcatgc agaagtactc cacactgcag
     5221 ggtccacctg gcaccggaaa gtcccacttc gctatcggac tggctctgta ctacccctcc
     5281 gctcgtatcg tgtacaccgc ttgctctcac gctgctgtgg acgctctgtg cgagaaggct
     5341 ctgaagtacc tgcctatcga caagtgctcc cgtatcatcc ccgctcgtgc tcgtgtggaa
     5401 tgcttcgaca agttcaaagt gaacagcacc ctcgagcagt acgtgttctg caccgtgaac
     5461 gctctgcccg agactaccgc tgacatcgtg gtgttcgacg agatctccat ggctaccaac
     5521 tacgacctgt ctgtggtcaa cgctcgtctg cgtgctaaac actacgtgta catcggcgac
     5581 cccgctcagt tgcctgctcc tcgtactctg ctgaccaagg gcactctgga acccgagtac
     5641 ttcaactccg tgtgccgtct gatgaagact atcggtcccg acatgttcct gggaacctgc
     5701 agacgttgcc ctgctgagat cgtggacacc gtgtctgctc tggtgtacga caacaagctc
     5761 aaggctcaca aggacaagtc cgctcagtgc ttcaagatgt tctacaaggg tgtcatcacc
     5821 cacgacgtgt cctccgctat caaccgtcct cagatcggtg tcgtgcgcga gttcctgact
     5881 cgtaatcctg cttggcgcaa ggctgtgttc atctcccctt acaactccca aaacgctgtg
     5941 gcttccaaga tcctgggcct gcctactcag accgtggact cctctcaggg ttccgagtac
     6001 gactacgtca tcttcaccca gactaccgag actgctcact cctgcaacgt gaaccgtttc
     6061 aacgtcgcca tcaccagggc taaagtcggt atcctgtgca tcatgtccga cagggacctc
     6121 tacgacaagc tccagttcac ctccttggag atccctcgtc gtaacgtggc gactctgcag
     6181 taaaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt
     6241 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     6301 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6361 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6421 atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctagaag atccggctgc
     6481 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactatcata
     6541 acccctagga gatccgaacc agataagtga aatctagttc caaactattt tgtcattttt
     6601 aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct
     6661 aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca
     6721 cagcggggca tttttcttcc tgttatgttt ttaatcaaac atcctgccaa ctccatgtga
     6781 caaaccgtca tcttcggcta cttt
//
