LOCUS       pLIB_GST-nsp13          7374 bp    DNA     circular SYN 22-MAR-2021
DEFINITION  
ACCESSION   pLIB_GST-nsp13
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Self
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     CDS             687..1547
                     /label="AmpR"
     misc_signal     2611..2835
                     /label="Tn7R"
     CDS             complement (2902..3435)
                     /label="GentaR"
     misc_signal     4132..4162
                     /label="polh promoter"
     CDS             4270..6753
                     /label="GST-nsp13"
     misc_signal     6822..7013
                     /label="SV40 late polyA"
     misc_signal     7127..7292
                     /label="Tn7L"
ORIGIN      
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cagctttgtt tgtactatca acaggttgaa ctgctgatca
     4021 acagatcctc tacgcggccg cggtaccata acttcgtata gcatacatta tacgaagtta
     4081 tctggtttta ctagtatcga ttcgcgacct actccggaat attaatagat catggagata
     4141 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
     4201 taataaaaaa acctataaat attccggatt attcataccg tcccaccatc gggcgcggat
     4261 ccagccacca tgtcccctat actaggttat tggaaaatta agggccttgt gcaacccact
     4321 cgacttcttt tggaatatct tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa
     4381 ggtgataaat ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa tcttccttat
     4441 tatattgatg gtgatgttaa attaacacag tctatggcca tcatacgtta tatagctgac
     4501 aagcacaaca tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga
     4561 gcggttttgg atattagata cggtgtttcg agaattgcat atagtaaaga ctttgaaact
     4621 ctcaaagttg attttcttag caagctacct gaaatgctga aaatgttcga agatcgttta
     4681 tgtcataaaa catatttaaa tggtgatcat gtaacccatc ctgacttcat gttgtatgac
     4741 gctcttgatg ttgttttata catggaccca atgtgcctgg atgcgttccc aaaattagtt
     4801 tgttttaaaa aacgtattga agctatccca caaattgata agtacttgaa atccagcaag
     4861 tatatagcat ggcctttgca gggctggcaa gccacgtttg gtggtggcga ccatcctcca
     4921 aaatcggatc tggttccgcg tggatccgct gttggtgctt gcgtgctgtg caactcccag
     4981 acctctttgc gttgcggtgc ttgtatccgt cgtccattcc tgtgctgcaa gtgctgctac
     5041 gaccacgtga tctccacctc tcacaagctg gtgctgtctg tgaaccccta cgtgtgcaac
     5101 gctcctggtt gcgacgtgac cgatgtgacc caactgtacc tcggtggcat gtcctactac
     5161 tgcaagtccc acaagcctcc aatcagcttc cctctgtgcg ctaacggaca ggtgttcggc
     5221 ctgtacaaga acacttgcgt gggttccgac aacgtcaccg acttcaacgc tatcgctacc
     5281 tgcgactgga ccaacgctgg cgactacatc ctggctaaca cttgcaccga gcgtctgaag
     5341 ctgttcgctg ctgagactct gaaggctacc gaggaaacct tcaagctgtc ctacggtatc
     5401 gccaccgtgc gtgaagtgct gtccgaccgt gaactgcacc tgtcctggga agtgggaaag
     5461 cctcgtcctc ctctgaaccg taactacgtg ttcaccggtt accgtgtgac caagaactcc
     5521 aaggtgcaga tcggcgagta caccttcgag aagggcgatt acggcgacgc tgtggtgtac
     5581 cgtggtacta ccacctacaa gctgaacgtg ggcgactact tcgtgctgac ttcccacacc
     5641 gtgatgcccc tgtctgctcc tactctggtg ccccaagagc actacgtgcg tatcaccgga
     5701 ctgtacccca ctctgaatat ctccgacgag ttctcctcca acgtggccaa ctaccagaaa
     5761 gtgggcatgc agaagtactc cacactgcag ggtccacctg gcaccggaaa gtcccacttc
     5821 gctatcggac tggctctgta ctacccctcc gctcgtatcg tgtacaccgc ttgctctcac
     5881 gctgctgtgg acgctctgtg cgagaaggct ctgaagtacc tgcctatcga caagtgctcc
     5941 cgtatcatcc ccgctcgtgc tcgtgtggaa tgcttcgaca agttcaaagt gaacagcacc
     6001 ctcgagcagt acgtgttctg caccgtgaac gctctgcccg agactaccgc tgacatcgtg
     6061 gtgttcgacg agatctccat ggctaccaac tacgacctgt ctgtggtcaa cgctcgtctg
     6121 cgtgctaaac actacgtgta catcggcgac cccgctcagt tgcctgctcc tcgtactctg
     6181 ctgaccaagg gcactctgga acccgagtac ttcaactccg tgtgccgtct gatgaagact
     6241 atcggtcccg acatgttcct gggaacctgc agacgttgcc ctgctgagat cgtggacacc
     6301 gtgtctgctc tggtgtacga caacaagctc aaggctcaca aggacaagtc cgctcagtgc
     6361 ttcaagatgt tctacaaggg tgtcatcacc cacgacgtgt cctccgctat caaccgtcct
     6421 cagatcggtg tcgtgcgcga gttcctgact cgtaatcctg cttggcgcaa ggctgtgttc
     6481 atctcccctt acaactccca aaacgctgtg gcttccaaga tcctgggcct gcctactcag
     6541 accgtggact cctctcaggg ttccgagtac gactacgtca tcttcaccca gactaccgag
     6601 actgctcact cctgcaacgt gaaccgtttc aacgtcgcca tcaccagggc taaagtcggt
     6661 atcctgtgca tcatgtccga cagggacctc tacgacaagc tccagttcac ctccttggag
     6721 atccctcgtc gtaacgtggc gactctgcag taaaagcttg tcgagaagta ctagaggatc
     6781 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     6841 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     6901 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6961 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga
     7021 tcactgcttg agcctagaag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     7081 tgctgccacc gctgagcaat aactatcata acccctagga gatccgaacc agataagtga
     7141 aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc
     7201 cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc
     7261 ctcccagttc ccaactattt tgtccgccca cagcggggca tttttcttcc tgttatgttt
     7321 ttaatcaaac atcctgccaa ctccatgtga caaaccgtca tcttcggcta cttt
//
