LOCUS       Exported                9058 bp ds-DNA     circular SYN 18-MAR-2021
DEFINITION  Parental vector for TOPO(R) TA cloning of long PCR products.
ACCESSION   .
VERSION     .
KEYWORDS    hp-50%GC-20bp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Thermo Fisher (Invitrogen)
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 23, 2021 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     primer_bind     1..89
                     /label=L_20GC50_F
     primer_bind     1..30
                     /label=L_4XBiotin_F (M13MP18-3520R)
     misc_feature    31..56
                     /label=GC clamp
     misc_feature    57..89
                     /label=M13MP18-3564F
     CDS             408..734
                     /codon_start=1
                     /gene="XI"
                     /product="pXI"
                     /label=M13 gene XI
                     /note="phage assembly"
                     /translation="MKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVVSQTY
                     DFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGNSNEI
                     VKCN"
     rep_origin      1980..2360
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2410..2490)
                     /label=L_20GC50_R
     primer_bind     complement(2410..2429)
                     /label=M13MP18-5936R
     misc_feature    2430..2451
                     /label=GC Clamp
     misc_feature    2452..2458
                     /label=Nb.BbvCI
     misc_feature    2463..2482
                     /label=Hairpin stem L
     primer_bind     2479..2575
                     /label=R_20GC50_F
     misc_feature    2479..2490
                     /label=BstXI
     misc_feature    2483..2486
                     /label=loop
     misc_feature    complement(2487..2506)
                     /label=Hairpin stem R
     misc_feature    complement(2510..2534)
                     /label=RecQ binding area
     misc_feature    2535..2541
                     /label=Nb.BbvCI
     primer_bind     2542..2575
                     /label=pBR322-31F
     CDS             2597..3787
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     CDS             4426..4617
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    4719..4859
                     /label=bom
                     /note="basis of mobility region from pBR322"
     misc_feature    4858..5009
                     /label=<<<<<<<<<<<672bp repeat in handles>>>>>>>>>>>>>
     misc_feature    5011..5529
                     /label=<<<<<<<<<<<672bp repeat in handles>>>>>>
     primer_bind     complement(5485..5529)
                     /label=R_20GC50_R
     primer_bind     complement(5494..5529)
                     /label=R_20GC50_DBCO_R (pBR322-3018R)
     misc_feature    5542..5554
                     /label=CAG is replaced withTGCAGATATCCAT
     primer_bind     complement(5655..5687)
                     /label=XLTOPO-148F
     primer_bind     complement(5655..5671)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5679..5695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5703..5733)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5748..5769
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5870..6021
                     /label=<<<<<<<<<<<672bp repeat in handles>>>>>>>>>>>>>
     misc_feature    6023..6541
                     /label=<<<<<<<<<<<672bp repeat in handles>>>>>>>>>>>>>
     rep_origin      complement(6057..6645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6497..6541)
                     /label=R_20GC50_R
     primer_bind     complement(6506..6541)
                     /label=R_20GC50_DBCO_R (pBR322-3018R)
     CDS             complement(6783..7157)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             complement(7364..8158)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(8507..8809)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8915..8940
                     /label=XLTOPO+144R
     primer_bind     8947..8963
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8970..8988
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcaataatcg gctgtctttc cttatcattc gggcgctcgg cgtgcagccg tgcgggcttg
       61 ttcaggactt atctattgtt gataaacagg cgcgttctgc attagctgaa catgttgttt
      121 attgtcgtcg tctggacaga attactttac cttttgtcgg tactttatat tctcttatta
      181 ctggctcgaa aatgcctctg cctaaattac atgttggcgt tgttaaatat ggcgattctc
      241 aattaagccc tactgttgag cgttggcttt atactggtaa gaatttgtat aacgcatatg
      301 atactaaaca ggctttttct agtaattatg attccggtgt ttattcttat ttaacgcctt
      361 atttatcaca cggtcggtat ttcaaaccat taaatttagg tcagaagatg aaattaacta
      421 aaatatattt gaaaaagttt tctcgcgttc tttgtcttgc gattggattt gcatcagcat
      481 ttacatatag ttatataacc caacctaagc cggaggttaa aaaggtagtc tctcagacct
      541 atgattttga taaattcact attgactctt ctcagcgtct taatctaagc tatcgctatg
      601 ttttcaagga ttctaaggga aaattaatta atagcgacga tttacagaag caaggttatt
      661 cactcacata tattgattta tgtactgttt ccattaaaaa aggtaattca aatgaaattg
      721 ttaaatgtaa ttaattttgt tttcttgatg tttgtttcat catcttcttt tgctcaggta
      781 attgaaatga ataattcgcc tctgcgcgat tttgtaactt ggtattcaaa gcaatcaggc
      841 gaatccgtta ttgtttctcc cgatgtaaaa ggtactgtta ctgtatattc atctgacgtt
      901 aaacctgaaa atctacgcaa tttctttatt tctgttttac gtgctaataa ttttgatatg
      961 gttggttcaa ttccttccat aattcagaag tataatccaa acaatcagga ttatattgat
     1021 gaattgccat catctgataa tcaggaatat gatgataatt ccgctccttc tggtggtttc
     1081 tttgttccgc aaaatgataa tgttactcaa acttttaaaa ttaataacgt tcgggcaaag
     1141 gatttaatac gagttgtcga attgtttgta aagtctaata cttctaaatc ctcaaatgta
     1201 ttatctattg acggctctaa tctattagtt gttagtgcac ctaaagatat tttagataac
     1261 cttcctcaat tcctttctac tgttgatttg ccaactgacc agatattgat tgagggtttg
     1321 atatttgagg ttcagcaagg tgatgcttta gatttttcat ttgctgctgg ctctcagcgt
     1381 ggcactgttg caggcggtgt taatactgac cgcctcacct ctgttttatc ttctgctggt
     1441 ggttcgttcg gtatttttaa tggcgatgtt ttagggctat cagttcgcgc attaaagact
     1501 aatagccatt caaaaatatt gtctgtgcca cgtattctta cgctttcagg tcagaagggt
     1561 tctatctctg ttggccagaa tgtccctttt attactggtc gtgtgactgg tgaatctgcc
     1621 aatgtaaata atccatttca gacgattgag cgtcaaaatg taggtatttc catgagcgtt
     1681 tttcctgttg caatggctgg cggtaatatt gttctggata ttaccagcaa ggccgatagt
     1741 ttgagttctt ctactcaggc aagtgatgtt attactaatc aaagaagtat tgctacaacg
     1801 gttaatttgc gtgatggaca gactctttta ctcggtggcc tcactgatta taaaaacact
     1861 tctcaagatt ctggcgtacc gttcctgtct aaaatccctt taatcggcct cctgtttagc
     1921 tcccgctctg attccaacga ggaaagcacg ttatacgtgc tcgtcaaagc aaccatagta
     1981 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     2041 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     2101 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     2161 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     2221 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     2281 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
     2341 agggattttg ccgatttcgg aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc
     2401 agcgtggacc gcttgctgca actctctcag cgggcacggc caggctcccg ccctcagcat
     2461 ttgagtcaac gtctggatcc atttttatgg atccagacgt tgactccccg ctgtctagag
     2521 gatccgacta tcgacctcag cgctttaatg cggtagttta tcacagttaa attgctaacg
     2581 cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac
     2641 cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat
     2701 cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat
     2761 gcaatttcta tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt
     2821 cctgctcgct tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt
     2881 cctgtggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     2941 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     3001 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
     3061 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     3121 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaccga tgcccttgag
     3181 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     3241 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat
     3301 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
     3361 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg
     3421 cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc
     3481 gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat
     3541 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca
     3601 gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt
     3661 cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc
     3721 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc
     3781 gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc
     3841 aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc
     3901 gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca
     3961 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
     4021 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
     4081 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
     4141 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
     4201 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
     4261 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
     4321 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
     4381 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
     4441 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
     4501 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
     4561 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     4621 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     4681 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     4741 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     4801 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     4861 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4921 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4981 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5041 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5101 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5161 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5221 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5281 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5341 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5401 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5461 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     5521 ttaccttcga agggcgaatt ctgcagatat ccatcacact ggcggccgtt actagtggat
     5581 ccgagctcgg taccaagctt gatgcatagc ttgagtattc taacgcgtca cctaaatagc
     5641 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5701 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     5761 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     5821 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     5881 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     5941 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6001 tgagcaaaag gccagcaaaa gcccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6061 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6481 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6661 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6721 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttagcacg
     6781 tgtcagtcct gctcctcggc cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg
     6841 aactcccgcc cccacggctg ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg
     6901 aagttcgtgg acacgacctc cgaccactcg gcgtacagct cgtccaggcc gcgcacccac
     6961 acccaggcca gggtgttgtc cggcaccacc tggtcctgga ccgcgctgat gaacagggtc
     7021 acgtcgtccc ggaccacacc ggcgaagtcg tcctccacga agtcccggga gaacccgagc
     7081 cggtcggtcc agaactcgac cgctccggcg acgtcgcgcg cggtgagcac cggaacggca
     7141 ctggtcaact tggccatggt ggccctcctc acgtgctatt attgaagcat ttatcagggt
     7201 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7261 ccgcgcacat ttccccgaaa agtgccacct gtatgcggtg tgaaataccg cacagatgcg
     7321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat aattcagaag aactcgtcaa
     7381 gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga
     7441 agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt
     7501 cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat
     7561 tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt
     7621 cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt
     7681 cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc
     7741 gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca
     7801 ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct
     7861 gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca
     7921 cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca
     7981 gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg
     8041 acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga
     8101 atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc
     8161 gaaacgatcc tcatcctgtc tcttgatcag agcttgatcc cctgcgccat cagatccttg
     8221 gcggcgagaa agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc
     8281 cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg
     8341 taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag
     8401 cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtga
     8461 aaaggatcta ggtgaagatc ctttttgata atctcatgcc tgacatttat attccccaga
     8521 acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt
     8581 ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc cagctttcat
     8641 ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc agacgtgcac
     8701 tggccagggg gatcaccatc cgtcgccccg gcgtgtcaat aatatcactc tgtacatcca
     8761 caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc cgtacgtata
     8821 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     8881 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     8941 gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc gaattgggcc
     9001 ctctagatgc atgctcgagc ggccgccagt gtgatggata tctgcagaat tcgccctt
//
