LOCUS       TVBB_C-term-mScarlet_(# 2756 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(46..906)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(255..276)
                     /label="YB104_TVBB_Seq_REV1"
                     /note="sequence: gcggataaagttgcaggaccac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          381..402
                     /label="YB105_TVBB_Seq_FWD1"
                     /note="sequence: gctcgtcgtttggtatggcttc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(768..789)
                     /label="YB106_TVBB_Seq_REV2"
                     /note="sequence: gcacgagtgggttacatcgaac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(838..906)
                     /label="AmpR signal peptide"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          879..900
                     /label="YB107_TVBB_Seq_FWD2"
                     /note="sequence: gggcgacacggaaatgttgaat"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(907..1011)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          995..1014
                     /label="YB115_TVUHA_Seq"
                     /note="sequence: caaataggggttccgcgcac"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1087..1093
                     /label="SapI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1110..1116
                     /label="SapI"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1118..1138
                     /label="Linker"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             1118..1138
                     /label="Translation 1118-1138"
     misc_feature    1139..1831
                     /label="mScarlet"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1139..1831
                     /label="Translation 1139-1831"
     primer          complement(1271..1292)
                     /label="YB108_TVBB_Seq_REV3"
                     /note="sequence: cacccttggtcaccttcagctt"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          1403..1424
                     /label="YB109_TVBB_Seq_FWD3"
                     /note="sequence: gagggcttcaagtgggagAGAg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1757..1778)
                     /label="YB110_TVBB_Seq_REV4"
                     /note="sequence: actgttccaccacggtgtagtc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             1832..1834
                     /label="Translation 1832-1834"
     primer          1921..1942
                     /label="YB113_TVBB_Seq_FWD5"
                     /note="sequence: tcagctcactcaaaggcggtaa"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1921..1942)
                     /label="YB114_TVDHA_Seq"
                     /note="sequence: ttaccgcctttgagtgagctga"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(2025..2707)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2099..2105)
                     /label="Kozak"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
       61 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
      121 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
      181 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
      241 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
      301 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
      361 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
      421 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
      481 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
      541 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
      601 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
      661 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
      721 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
      781 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
      841 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
      901 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
      961 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     1021 ccgaaaagtg ccacctgatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     1081 gcTACGGAAG AGCGCGATCG CGTTTAAAGG CTCTTCAGGC GGAGGCGGCA GCGCCAGCgt
     1141 gagcaagggc gaggcagtga tcaaggagtt catgAGAttc aaggtgcaca tggagggctc
     1201 catgaacggc cacgagttcg agatcgaggg cgagggcgag ggcAGAccct acgagggcac
     1261 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttctcct gggacatcct
     1321 gtcccctcag ttcatgtacg gctccagggc cttcaccaag caccccgccg acatccccga
     1381 ctactataag cagtccttcc ccgagggctt caagtgggag AGAgtgatga acttcgagga
     1441 cggcggcgcc gtgaccgtga cccaggacac ctccctggag gacggcaccc tgatctacaa
     1501 ggtgaagctc AGAggcacca acttccctcc tgacggcccc gtaatgcaga agaagacaat
     1561 gggctgggaa gcatccaccg agAGAttgta ccccgaggac ggcgtgctga agggcgacat
     1621 taagatggcc ctgAGActga aggacggcgg ccgctacctg gcggacttca agaccaccta
     1681 caaggccaag aagcccgtgc agatgcccgg cgcctacaac gtcgacAGGa agttggacat
     1741 cacctcccac aacgaggact acaccgtggt ggaacagtac gaacgctccg agggcAGAca
     1801 ctccaccggc ggcatggacg agctgtacaa gTGACGAAGA GCGTTTAAAC GCGAACCGGC
     1861 TCTTCGAATt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1921 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1981 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2401 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2461 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2521 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2581 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2641 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2701 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaag
//