LOCUS       TVBB_C-term-mScarlet-Pu 3419 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..693
                     /label="mScarlet"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1..693
                     /label="Translation 1-693"
     primer          complement(133..154)
                     /label="YB108_TVBB_Seq_REV3"
                     /note="sequence: cacccttggtcaccttcagctt"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          265..286
                     /label="YB109_TVBB_Seq_FWD3"
                     /note="sequence: gagggcttcaagtgggagAGAg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(619..640)
                     /label="YB110_TVBB_Seq_REV4"
                     /note="sequence: actgttccaccacggtgtagtc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    694..702
                     /label="P2A linker"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    694..759
                     /label="Optimized P2A (Chiu-An Lo et al.)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             694..759
                     /label="Translation 694-759"
     primer          739..759
                     /label="YB111_TVBB_Seq_FWD4"
                     /note="sequence: GTGGAGGAAAACCCTGGACCT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    760..1356
                     /label="Puro"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             760..1356
                     /label="Translation 760-1356"
     primer          complement(806..827)
                     /label="YB118_TVBB_Seq"
                     /note="sequence: AGGGTTCTTACGGCTCTAGGGA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             1357..1359
                     /label="Translation 1357-1359"
     misc_feature    1361..1367
                     /label="SapI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(1361..1382)
                     /label="YB112_TVBB_Seq_REV5"
                     /note="sequence: GGTTCGCGTTTAAACGCTCTTC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1384..1390
                     /label="SapI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1446..1467
                     /label="YB113_TVBB_Seq_FWD5"
                     /note="sequence: tcagctcactcaaaggcggtaa"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1446..1467)
                     /label="YB114_TVDHA_Seq"
                     /note="sequence: ttaccgcctttgagtgagctga"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(1550..2232)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1624..1630)
                     /label="Kozak"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2327..3187)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2536..2557)
                     /label="YB104_TVBB_Seq_REV1"
                     /note="sequence: gcggataaagttgcaggaccac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2662..2683
                     /label="YB105_TVBB_Seq_FWD1"
                     /note="sequence: gctcgtcgtttggtatggcttc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3049..3070)
                     /label="YB106_TVBB_Seq_REV2"
                     /note="sequence: gcacgagtgggttacatcgaac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(3119..3187)
                     /label="AmpR signal peptide"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          3160..3181
                     /label="YB107_TVBB_Seq_FWD2"
                     /note="sequence: gggcgacacggaaatgttgaat"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(3188..3292)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          3276..3295
                     /label="YB115_TVUHA_Seq"
                     /note="sequence: caaataggggttccgcgcac"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(3339..3363)
                     /label="YB94_AAVTV_BB_Rev"
                     /note="sequence: gcggtattttctccttacgcatctg"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     misc_feature    3368..3374
                     /label="SapI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3391..3397
                     /label="SapI"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3399..3419
                     /label="Linker"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             3399..3419
                     /label="Translation 3399-3419"
ORIGIN
        1 gtgagcaagg gcgaggcagt gatcaaggag ttcatgAGAt tcaaggtgca catggagggc
       61 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggcAGAcc ctacgagggc
      121 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttctc ctgggacatc
      181 ctgtcccctc agttcatgta cggctccagg gccttcacca agcaccccgc cgacatcccc
      241 gactactata agcagtcctt ccccgagggc ttcaagtggg agAGAgtgat gaacttcgag
      301 gacggcggcg ccgtgaccgt gacccaggac acctccctgg aggacggcac cctgatctac
      361 aaggtgaagc tcAGAggcac caacttccct cctgacggcc ccgtaatgca gaagaagaca
      421 atgggctggg aagcatccac cgagAGAttg taccccgagg acggcgtgct gaagggcgac
      481 attaagatgg ccctgAGAct gaaggacggc ggccgctacc tggcggactt caagaccacc
      541 tacaaggcca agaagcccgt gcagatgccc ggcgcctaca acgtcgacAG Gaagttggac
      601 atcacctccc acaacgagga ctacaccgtg gtggaacagt acgaacgctc cgagggcAGA
      661 cactccaccg gcggcatgga cgagctgtac aagGGAAGCG GAGCGACGAA TTTTAGTCTA
      721 CTGAAACAAG CGGGAGACGT GGAGGAAAAC CCTGGACCTA TGACCGAGTA CAAGCCCACG
      781 GTGCGCCTCG CCACCAGAGA CGACGTCCCT AGAGCCGTAA GAACCCTCGC CGCCGCTTTT
      841 GCCGACTACC CTGCCACAAG ACACACCGTC GATCCGGACA GGCACATCGA GAGAGTCACC
      901 GAGCTGCAAG AACTCTTCCT CACGAGGGTC GGGCTCGACA TCGGAAAGGT GTGGGTCGCG
      961 GACGACGGAG CCGCGGTGGC TGTCTGGACC ACGCCTGAGA GTGTCGAAGC AGGGGCAGTG
     1021 TTCGCCGAAA TCGGCCCAAG AATGGCCGAA TTGTCTGGTT CCCGGCTGGC AGCTCAGCAA
     1081 CAGATGGAAG GCCTCCTGGC GCCACACCGG CCTAAGGAGC CCGCGTGGTT CCTGGCTACA
     1141 GTTGGAGTTT CACCAGATCA TCAGGGAAAG GGTCTGGGCT CTGCTGTCGT GCTCCCAGGA
     1201 GTGGAAGCTG CTGAGAGAGC CGGGGTGCCT GCCTTCCTGG AGACCTCTGC GCCTAGGAAT
     1261 CTCCCCTTCT ACGAGAGACT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA
     1321 CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAC GAAGAGCGTT TAAACGCGAA
     1381 CCGGCTCTTC GAATtcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1441 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1501 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1561 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1621 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1681 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1741 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1801 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1861 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1921 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1981 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     2041 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2101 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2161 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2221 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2281 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2341 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2401 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2461 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2521 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2581 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2641 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2701 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2761 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2821 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2881 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2941 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3001 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3061 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3301 cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     3361 cgcTACGGAA GAGCGCGATC GCGTTTAAAG GCTCTTCAGG CGGAGGCGGC AGCGCCAGC
//