LOCUS       TVBB_C-term-mScarlet-Bl 3218 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(46..906)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(255..276)
                     /label="YB104_TVBB_Seq_REV1"
                     /note="sequence: gcggataaagttgcaggaccac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          381..402
                     /label="YB105_TVBB_Seq_FWD1"
                     /note="sequence: gctcgtcgtttggtatggcttc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(768..789)
                     /label="YB106_TVBB_Seq_REV2"
                     /note="sequence: gcacgagtgggttacatcgaac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(838..906)
                     /label="AmpR signal peptide"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          879..900
                     /label="YB107_TVBB_Seq_FWD2"
                     /note="sequence: gggcgacacggaaatgttgaat"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(907..1011)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          995..1014
                     /label="YB115_TVUHA_Seq"
                     /note="sequence: caaataggggttccgcgcac"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1087..1093
                     /label="SapI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1110..1116
                     /label="SapI"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1118..1138
                     /label="Linker"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             1118..1138
                     /label="Translation 1118-1138"
     misc_feature    1139..1831
                     /label="mScarlet"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1139..1831
                     /label="Translation 1139-1831"
     primer          complement(1271..1292)
                     /label="YB108_TVBB_Seq_REV3"
                     /note="sequence: cacccttggtcaccttcagctt"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          1403..1424
                     /label="YB109_TVBB_Seq_FWD3"
                     /note="sequence: gagggcttcaagtgggagAGAg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1757..1778)
                     /label="YB110_TVBB_Seq_REV4"
                     /note="sequence: actgttccaccacggtgtagtc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1832..1840
                     /label="P2A linker"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1832..1897
                     /label="Optimized P2A (Chiu-An Lo et al.)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             1832..1897
                     /label="Translation 1832-1897"
     primer          1877..1897
                     /label="YB111_TVBB_Seq_FWD4"
                     /note="sequence: GTGGAGGAAAACCCTGGACCT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             1898..2293
                     /label="Translation 1898-2293"
     misc_feature    1901..2293
                     /label="Blast resistance"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             2294..2296
                     /label="Translation 2294-2296"
     primer          complement(2298..2319)
                     /label="YB112_TVBB_Seq_REV5"
                     /note="sequence: GGTTCGCGTTTAAACGCTCTTC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2383..2404
                     /label="YB113_TVBB_Seq_FWD5"
                     /note="sequence: tcagctcactcaaaggcggtaa"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2383..2404)
                     /label="YB114_TVDHA_Seq"
                     /note="sequence: ttaccgcctttgagtgagctga"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(2487..3169)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2561..2567)
                     /label="Kozak"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
       61 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
      121 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
      181 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
      241 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
      301 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
      361 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
      421 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
      481 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
      541 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
      601 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
      661 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
      721 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
      781 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
      841 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
      901 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
      961 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     1021 ccgaaaagtg ccacctgatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     1081 gcTACGGAAG AGCGCGATCG CGTTTAAAGG CTCTTCAGGC GGAGGCGGCA GCGCCAGCgt
     1141 gagcaagggc gaggcagtga tcaaggagtt catgAGAttc aaggtgcaca tggagggctc
     1201 catgaacggc cacgagttcg agatcgaggg cgagggcgag ggcAGAccct acgagggcac
     1261 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttctcct gggacatcct
     1321 gtcccctcag ttcatgtacg gctccagggc cttcaccaag caccccgccg acatccccga
     1381 ctactataag cagtccttcc ccgagggctt caagtgggag AGAgtgatga acttcgagga
     1441 cggcggcgcc gtgaccgtga cccaggacac ctccctggag gacggcaccc tgatctacaa
     1501 ggtgaagctc AGAggcacca acttccctcc tgacggcccc gtaatgcaga agaagacaat
     1561 gggctgggaa gcatccaccg agAGAttgta ccccgaggac ggcgtgctga agggcgacat
     1621 taagatggcc ctgAGActga aggacggcgg ccgctacctg gcggacttca agaccaccta
     1681 caaggccaag aagcccgtgc agatgcccgg cgcctacaac gtcgacAGGa agttggacat
     1741 cacctcccac aacgaggact acaccgtggt ggaacagtac gaacgctccg agggcAGAca
     1801 ctccaccggc ggcatggacg agctgtacaa gGGAAGCGGA GCGACGAATT TTAGTCTACT
     1861 GAAACAAGCG GGAGACGTGG AGGAAAACCC TGGACCTATG GCCAAGCCTT TGTCTCAAGA
     1921 AGAATCCACC CTCATTGAAA GAGCAACGGC TACAATCAAC AGCATCCCCA TCTCTGAAGA
     1981 CTACAGCGTC GCCAGCGCAG CTCTCTCTAG CGACGGCAGG ATCTTCACTG GTGTCAATGT
     2041 ATATCATTTT ACTGGGGGAC CTTGTGCAGA ACTCGTGGTG CTGGGCACTG CTGCTGCTGC
     2101 GGCAGCTGGC AACCTGACTT GTATCGTCGC GATCGGAAAT GAGAACAGGG GCATCTTGAG
     2161 CCCCTGCGGA AGATGCCGAC AGGTGCTTCT CGATCTGCAT CCTGGGATCA AAGCCATAGT
     2221 GAAGGACAGT GATGGACAGC CGACGGCAGT TGGGATTCGT GAATTGCTGC CCTCTGGTTA
     2281 TGTGTGGGAG GGCTGACGAA GAGCGTTTAA ACGCGAACCG GCTCTTCGAA Ttcctcgctc
     2341 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2401 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2461 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2521 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2581 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2641 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2701 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2761 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2821 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2881 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2941 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3001 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3061 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3121 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3181 aggatcttca cctagatcct tttaaattaa aaatgaag
//