LOCUS       TVBB_C-term-mTurquoise2 2777 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             7..9
                     /label="Translation 7-9"
     misc_feature    11..17
                     /label="SapI site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(11..32)
                     /label="YB112_TVBB_Seq_REV5"
                     /note="sequence: GGTTCGCGTTTAAACGCTCTTC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    34..40
                     /label="SapI site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          96..117
                     /label="YB113_TVBB_Seq_FWD5"
                     /note="sequence: tcagctcactcaaaggcggtaa"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      complement(200..882)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(274..280)
                     /label="Kozak"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(977..1837)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1186..1207)
                     /label="YB104_TVBB_Seq_REV1"
                     /note="sequence: gcggataaagttgcaggaccac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          1312..1333
                     /label="YB105_TVBB_Seq_FWD1"
                     /note="sequence: gctcgtcgtttggtatggcttc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1699..1720)
                     /label="YB106_TVBB_Seq_REV2"
                     /note="sequence: gcacgagtgggttacatcgaac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(1769..1837)
                     /label="AmpR signal peptide"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          1810..1831
                     /label="YB107_TVBB_Seq_FWD2"
                     /note="sequence: gggcgacacggaaatgttgaat"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(1838..1942)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2018..2024
                     /label="SapI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2041..2047
                     /label="SapI"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2049..2069
                     /label="Linker"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             2049..2069
                     /label="Translation 2049-2069"
     misc_feature    join(2070..2777,1..6)
                     /label="mTurquoise2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             join(2070..2777,1..6)
                     /label="Translation 2070-6"
     primer          complement(2363..2384)
                     /label="YB119_TVBB_Seq"
                     /note="sequence: GTTGCCGTCGTCCTTGAAGAAG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2481..2502
                     /label="YB120_TVBB_Seq"
                     /note="sequence: GGGCACAAGCTGGAGTACAACT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 TACAAGTGAC GAAGAGCGTT TAAACGCGAA CCGGCTCTTC GAATtcctcg ctcactgact
       61 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      121 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      181 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      241 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      301 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      361 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      421 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      481 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      541 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
      601 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
      661 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
      721 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
      781 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
      841 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
      901 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
      961 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     1021 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     1081 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     1141 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     1201 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     1261 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     1321 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     1381 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     1441 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     1501 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     1561 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     1621 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     1681 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     1741 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     1801 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     1861 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     1921 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgat gcggtgtgaa
     1981 ataccgcaca gatgcgtaag gagaaaatac cgcTACGGAA GAGCGCGATC GCGTTTAAAG
     2041 GCTCTTCAGG CGGAGGCGGC AGCGCCAGCG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG
     2101 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAATGG CCACAAGTTC AGCGTGTCCG
     2161 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG
     2221 GAAAGCTGCC TGTGCCCTGG CCAACCCTCG TGACCACCCT GTCTTGGGGC GTGCAGTGCT
     2281 TCGCCAGATA CCCTGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCTGCC ATGCCCGAAG
     2341 GCTACGTCCA GGAGAGGACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG
     2401 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACAGAATCGA GCTGAAGGGC ATCGACTTCA
     2461 AGGAGGACGG AAACATCCTG GGGCACAAGC TGGAGTACAA CTACtTCTCT GACAACGTCT
     2521 ATATCACCGC CGACAAGCAG AAGAATGGCA TCAAGGCCAA CTTCAAGATC CGCCACAACA
     2581 TCGAGGATGG CGGAGTGCAG CTCGCCGATC ACTACCAGCA GAATACCCCT ATCGGCGACG
     2641 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCAAGCTG AGCAAAGACC
     2701 CCAACGAGAA GAGAGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC
     2761 TCGGAATGGA CGAGCTG
//