LOCUS       TVBB_C-term-mNeongreen- 3431 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(46..906)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(255..276)
                     /label="YB104_TVBB_Seq_REV1"
                     /note="sequence: gcggataaagttgcaggaccac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          381..402
                     /label="YB105_TVBB_Seq_FWD1"
                     /note="sequence: gctcgtcgtttggtatggcttc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(768..789)
                     /label="YB106_TVBB_Seq_REV2"
                     /note="sequence: gcacgagtgggttacatcgaac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(838..906)
                     /label="AmpR signal peptide"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          879..900
                     /label="YB107_TVBB_Seq_FWD2"
                     /note="sequence: gggcgacacggaaatgttgaat"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(907..1011)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1087..1093
                     /label="SapI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1110..1116
                     /label="SapI"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(1117..1138)
                     /label="TVBB_RVlong"
                     /note="sequence: GCTGGCGCTGCCGCCTCCGCCT"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1118..1138
                     /label="Linker"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             1118..1138
                     /label="Translation 1118-1138"
     primer          complement(1123..1138)
                     /label="TVBB_RVshort"
                     /note="sequence: GCTGGCGCTGCCGCCT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1139..1843
                     /label="mNeonGreen"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1139..1843
                     /label="Translation 1139-1843"
     primer          complement(1158..1179)
                     /label="YB116_TVBB_Seq"
                     /note="sequence: gctgggagagaggccatgttat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          1435..1456
                     /label="YB117_TVBB_Seq"
                     /note="sequence: gcagtttgaagatggtgcctcc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1779..1800
                     /label="[S/S] mNeongreen-P2A-Puro [S/S] 1779-1800 FWD 4"
                     /note="sequence: agctcaacttcaaggagtggca"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1844..1852
                     /label="P2A linker"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          1844..1871
                     /label="TVBB_FW"
                     /note="sequence: GGAAGCGGAGCGACGAATTTTAGTCTAC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1844..1909
                     /label="Optimized P2A (Chiu-An Lo et al.)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             1844..1909
                     /label="Translation 1844-1909"
     primer          1889..1909
                     /label="YB111_TVBB_Seq_FWD4"
                     /note="sequence: GTGGAGGAAAACCCTGGACCT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1910..2506
                     /label="Puro"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             1910..2506
                     /label="Translation 1910-2506"
     primer          complement(1956..1977)
                     /label="YB118_TVBB_Seq"
                     /note="sequence: AGGGTTCTTACGGCTCTAGGGA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2145..2165)
                     /label="[S/S] mNeongreen-P2A-Puro [S/S] 2145-2165 REV 5"
                     /note="sequence: CCCCTGCTTCGACACTCTCAG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2277..2298
                     /label="[S/S] mNeongreen-P2A-Puro [S/S] 2277-2298 FWD 5"
                     /note="sequence: GGTTCCTGGCTACAGTTGGAGT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             2507..2509
                     /label="Translation 2507-2509"
     misc_feature    2511..2517
                     /label="SapI site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2511..2532)
                     /label="YB112_TVBB_Seq_REV5"
                     /note="sequence: GGTTCGCGTTTAAACGCTCTTC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2534..2540
                     /label="SapI site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2596..2617
                     /label="YB113_TVBB_Seq_FWD5"
                     /note="sequence: tcagctcactcaaaggcggtaa"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      complement(2700..3382)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2774..2780)
                     /label="Kozak"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
       61 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
      121 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
      181 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
      241 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
      301 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
      361 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
      421 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
      481 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
      541 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
      601 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
      661 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
      721 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
      781 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
      841 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
      901 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
      961 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     1021 ccgaaaagtg ccacctgatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     1081 gcTACGGAAG AGCGCGATCG CGTTTAAAGG CTCTTCAGGC GGAGGCGGCA GCGCCAGCgt
     1141 gagcaagggc gaggaggata acatggcctc tctcccagcg acacatgagt tacacatctt
     1201 tggcTCTatc aacggtgtgg actttgacat ggtgggtcag GGAaccGGAa atccaaatga
     1261 tggttatgag gagttaAATc tgaagTCAac caagggtgac ctccagttcT CTCCTtggat
     1321 tctggtccct catatcgggt atggcttcca tcagtacctg CCTtaccctg acgggatgTC
     1381 Tcctttccag gccgccatgg tagatggcTC Tggataccaa gtccatAGAa caatgcagtt
     1441 tgaagatggt gcctccctta ctgttAATta cAGAtacACA tacgagggaa gcCATatcaa
     1501 aggagaggcc caggtgaagg ggactggttt ccctgctgac ggtcctgtga tgaccaactc
     1561 gctgaccgct gcggactggt gcaggtcgaa gAAAacttac cccaacgaca aaaccatcat
     1621 cagtaccttt aagtggagtt acaccactgg aaatggcAAA AGAtaccgga gcactgcgAG
     1681 AaccACTtac ACTtttgcca agccaatggc ggctaactat ctgaagaacc agccgatgta
     1741 cgtgttccgt aagacggagc tcaagcactc caagaccgag ctcaacttca aggagtggca
     1801 aaaggccttt accgatgtga tgggcatgga cgagctgtac aagGGAAGCG GAGCGACGAA
     1861 TTTTAGTCTA CTGAAACAAG CGGGAGACGT GGAGGAAAAC CCTGGACCTA TGACCGAGTA
     1921 CAAGCCCACG GTGCGCCTCG CCACCAGAGA CGACGTCCCT AGAGCCGTAA GAACCCTCGC
     1981 CGCCGCTTTT GCCGACTACC CTGCCACAAG ACACACCGTC GATCCGGACA GGCACATCGA
     2041 GAGAGTCACC GAGCTGCAAG AACTCTTCCT CACGAGGGTC GGGCTCGACA TCGGAAAGGT
     2101 GTGGGTCGCG GACGACGGAG CCGCGGTGGC TGTCTGGACC ACGCCTGAGA GTGTCGAAGC
     2161 AGGGGCAGTG TTCGCCGAAA TCGGCCCAAG AATGGCCGAA TTGTCTGGTT CCCGGCTGGC
     2221 AGCTCAGCAA CAGATGGAAG GCCTCCTGGC GCCACACCGG CCTAAGGAGC CCGCGTGGTT
     2281 CCTGGCTACA GTTGGAGTTT CACCAGATCA TCAGGGAAAG GGTCTGGGCT CTGCTGTCGT
     2341 GCTCCCAGGA GTGGAAGCTG CTGAGAGAGC CGGGGTGCCT GCCTTCCTGG AGACCTCTGC
     2401 GCCTAGGAAT CTCCCCTTCT ACGAGAGACT CGGCTTCACC GTCACCGCCG ACGTCGAGGT
     2461 GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAC GAAGAGCGTT
     2521 TAAACGCGAA CCGGCTCTTC GAATtcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2581 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2641 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2701 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2761 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2821 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2881 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2941 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3001 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3061 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3121 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3181 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3241 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3301 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3361 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3421 taaaaatgaa g
//