LOCUS       pEXP5-NT/6xHis_m        3562 bp ds-DNA     circular     25-MAY-2021
DEFINITION  T7 RNAP constitutive expression with 6xHis mCHERRY F30 Broccoli
            aptamer.
ACCESSION   urn.local.gonzales.x-deo92wt
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     RBS             66..71
     misc_feature    79..858
                     /note="Geneious type: gene"
                     /standard_name="6xHis mCHERRY"
     primer_bind     complement(850..1005)
                     /standard_name="F30-Broccoli EX Ultramer"
     primer_bind     complement(850..866)
                     /standard_name="mCHERRY Rev"
     misc_feature    871..975
                     /note="Geneious type: motif"
                     /standard_name="F30 Broccoli"
     terminator      976..1094
                     /standard_name="Tphi terminator"
     misc_feature    990..3218
                     /standard_name="Part 2"
     primer_bind     990..1007
                     /standard_name="Tphi Fwd"
     CDS             1513..2172
                     /standard_name="AmpR"
     rep_origin      2270..2952
     misc_feature    3133..1005
                     /standard_name="Part 1 + F30-Broccoli ultramer"
     misc_feature    3133..866
                     /standard_name="Part 1"
     primer_bind     3133..3151
                     /standard_name="NFB pTetO gBlock Fwd"
     primer_bind     complement(3201..3218)
                     /standard_name="DUR Vector 2"
     promoter        3562..18
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /standard_name="T7 promoter"
ORIGIN      
        1 aatacgactc actataggga gaccacaacg gtttccctct agaaataatt ttgtttaact
       61 ttaagaagga gatataccat gtctggttct catcatcatc atcatcatgg tagcagcggc
      121 gaaaacctgt attttcagtc catggtgagc aagggcgagg aggataacat ggccatcatc
      181 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      241 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      301 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
      361 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      421 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      481 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
      541 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
      601 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
      661 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
      721 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
      781 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      841 ctgtacaagg gcagctaaag ggtgatccgg ttgccatgtg tatgtgggag acggtcgggt
      901 ccagatattc gtatctgtcg agtagagtgt gggctcccac atactctgat gatccttcgg
      961 gatcattcat ggcaactgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     1021 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     1081 tgaaaggagg aactatatcc ggataattct tgaagacgaa agggcctcgt gatacgccta
     1141 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     1201 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1261 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1321 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1381 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1441 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1501 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     1561 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1621 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1681 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1741 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1801 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1861 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1921 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1981 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     2041 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2101 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2161 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2221 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2281 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2341 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2401 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2461 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2521 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2581 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2641 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2701 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2761 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2821 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2881 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2941 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3001 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3061 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3121 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc
     3181 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     3241 cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat
     3301 ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa
     3361 accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca
     3421 cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag
     3481 ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc
     3541 cgagatctcg atcccgcgaa at
//
