LOCUS       Exported               11313 bp ds-DNA     circular SYN 31-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Taylor Cook
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 23, 2021 from SnapGene 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11313
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     complement(14..60)
                     /label=JMR451
                     /note="RK2 ori Reverse :: pMP11"
     terminator      30..38
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     38..80
                     /label=JMR462
                     /note="Gent R :: pJMR50/51"
     primer_bind     complement(38..60)
                     /label=JMR460
                     /note="pJMR50 Forward"
     primer_bind     38..60
                     /label=JMR452
                     /note="pMP11 REverse"
     CDS             complement(51..60)
                     /codon_start=1
                     /label=beta-lactamase
                     /note="bla; ampicillin resistance"
                     /translation="HW"
     primer_bind     61..80
                     /label=JMR339
     CDS             complement(74..1250)
                     /label=GmR
     primer_bind     170..189
                     /label=GG146
                     /note="R Gmr specific"
     primer_bind     241..267
                     /label=rMP331
     primer_bind     241..259
                     /label=rMP320
     primer_bind     243..261
                     /label=JC185
     primer_bind     complement(251..267)
                     /label=A0061 BB F (2)
                     /note="ALM289"
     primer_bind     complement(251..267)
                     /label=A0061 BB F
                     /note="REC"
     primer_bind     complement(253..268)
                     /label=JMR458
                     /note="pMP11 Forward - Red gene deletion"
     primer_bind     complement(311..332)
                     /label=JMR340
                     /note="pJMR2_cPCR_F_1"
     CDS             complement(333..866)
                     /codon_start=1
                     /label=gentamicin-3-acetyltransferase
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEVVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     333..353
                     /label=gmR_R
                     /note="JT"
     primer_bind     complement(415..434)
                     /label=GG145
                     /note="F Gmr specific (seq when Gmr upstream)"
     primer_bind     418..437
                     /label=CMJ135
                     /note="gentR inside bottom rev"
     primer_bind     complement(421..442)
                     /label=rTBC0477
     primer_bind     570..589
                     /label=JC196
     primer_bind     773..792
                     /label=JC199
     primer_bind     complement(846..866)
                     /label=gmR_F
                     /note="JT"
     primer_bind     complement(865..876)
                     /label=gmR_F
                     /note="JT"
     misc_feature    923..941
                     /label=CMJ134
                     /note="CMJ134"
     primer_bind     complement(923..941)
                     /label=GentR top fwd
                     /note="ALM546 homology with CMJ134"
     primer_bind     923..941
                     /label=CMJ134
                     /note="gentR inside top rev"
     primer_bind     complement(987..1006)
                     /label=aadA-FWD
                     /note="DMP"
     primer_bind     1012..1033
                     /label=JMR345
                     /note="pJMR2_cPCR_R_3"
     promoter        complement(1055..1083)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    1055..1063
                     /label=-10 box
                     /note="-10 box"
     primer_bind     complement(1069..1091)
                     /label=GmR_F
     primer_bind     1071..1091
                     /label=glpK GmR R seq
     misc_feature    1078..1083
                     /label=-35 box
                     /note="-35 box"
     primer_bind     1178..1197
                     /label=JCC391
     primer_bind     complement(1224..1251)
                     /label=Fragment 1.FOR
     primer_bind     complement(1231..1270)
                     /label=JMR461
                     /note="Gent Forward :: pJMR50/51"
     primer_bind     complement(1231..1251)
                     /label=GmR Fwd
                     /note="ALM559"
     primer_bind     complement(1231..1251)
                     /label=JMR338
     primer_bind     1251..1275
                     /label=rMP347
     primer_bind     1251..1271
                     /label=*FuncBio* SL1
                     /note="This is a free sequencing primer offered by 
                     Functional Biosciences"
     primer_bind     1251..1270
                     /label=rMP346
     primer_bind     1259..1276
                     /label=iG_cPCR-P1xx_F
                     /note="60.6C"
     primer_bind     complement(1271..1291)
                     /label=pBad35NP_R
                     /note="JT"
     primer_bind     complement(1273..1294)
                     /label=GibATW_pBT2-3'F
                     /note="MFC"
     primer_bind     complement(1274..1294)
                     /label=rMP427
     primer_bind     complement(1274..1294)
                     /label=rMP165 (2)
     primer_bind     complement(1274..1294)
                     /label=rMP165
     primer_bind     complement(1275..1334)
                     /label=rMP261-FLAG
     primer_bind     complement(1275..1294)
                     /label=rMP261
     primer_bind     1291..1350
                     /label=rMP260-FLAG
     misc_feature    1295..1330
                     /label=FLAG tag /w G-S-G linker
                     /note="FLAG tag /w G-S-G linker"
     primer_bind     1298..1357
                     /label=rMP308
     CDS             complement(1331..5434)
                     /codon_start=1
                     /label=wtCas9
                     /note="wtCas9"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     1360..1378
                     /label=rMP234
     primer_bind     1579..1600
                     /label=GG184
                     /note="R seq end dCas9"
     primer_bind     complement(1697..1716)
                     /label=rMP225
     primer_bind     1866..1885
                     /label=rMP233
     primer_bind     complement(2195..2212)
                     /label=rMP224
     primer_bind     2347..2366
                     /label=rMP232
     primer_bind     complement(2662..2682)
                     /label=dCas9 Seq F3
                     /note="ALM566"
     primer_bind     complement(2698..2716)
                     /label=rMP223
     primer_bind     2866..2885
                     /label=rMP231
     misc_feature    2915..2917
                     /label=HNH like active site
                     /note="HNH like active site"
     primer_bind     complement(3002..3023)
                     /label=GG183
                     /note="F seq mid dCas9"
     primer_bind     complement(3200..3219)
                     /label=rMP222
     primer_bind     3333..3350
                     /label=rMP230
     primer_bind     3407..3430
                     /label=dCas9 Seq R
                     /note="ALM565"
     primer_bind     complement(3407..3430)
                     /label=dCas9 Seq F2
                     /note="ALM564"
     primer_bind     complement(3637..3661)
                     /label=GG182
                     /note="F seq mid dCas9"
     primer_bind     complement(3665..3684)
                     /label=rMP221
     primer_bind     3840..3859
                     /label=rMP229
     primer_bind     complement(4194..4211)
                     /label=rMP220
     primer_bind     4343..4362
                     /label=rMP228
     primer_bind     complement(4639..4661)
                     /label=dCas9 Seq F1
                     /note="ALM563"
     primer_bind     complement(4699..4716)
                     /label=rMP219
     primer_bind     4848..4867
                     /label=rMP227
     primer_bind     4980..4999
                     /label=GG190
                     /note="R beg dCas9 seq"
     primer_bind     complement(5197..5218)
                     /label=GG170
                     /note="seq mid Cas9"
     primer_bind     5332..5350
                     /label=rMP226
     misc_feature    5405..5407
                     /label=RuvC-like active site
                     /note="RuvC-like active site"
     primer_bind     complement(5407..5426)
                     /label=rMP218
     primer_bind     complement(5408..5464)
                     /label=rMP259
     primer_bind     5435..5454
                     /label=rMP262
     misc_feature    5443..5448
                     /label=RBS
                     /note="RBS"
     primer_bind     5449..5466
                     /label=rMP195
     primer_bind     complement(5451..5508)
                     /label=rMP399
     misc_feature    5468
                     /label=TSP
                     /note="TSP"
     misc_feature    5469..5528
                     /label=J23105 promoter, -60 to -1
                     /note="J23105 promoter, -60 to -1"
     misc_feature    5470..5504
                     /label=J23105 Variable region
                     /note="J23105 Variable region"
     primer_bind     5476..5520
                     /label=MC111
                     /note="63C, p105 RC"
     primer_bind     complement(5476..5504)
                     /label=MC116
                     /note="cPCR p105F"
     primer_bind     5489..5546
                     /label=rMP397
     primer_bind     complement(5505..5526)
                     /label=bio_uni_F
     primer_bind     5505..5526
                     /label=bbvector-linear R
                     /note="RL (igem)"
     primer_bind     complement(5508..5528)
                     /label=rMP309
     terminator      5557..5822
                     /label=rrnB_terminator
     primer_bind     5557..5574
                     /label=rMP292
     primer_bind     5560..5577
                     /label=rMP392
     primer_bind     5562..5586
                     /label=g-pbba5c seq r
                     /note="RL (igem)"
     terminator      5576..5619
                     /label=rrnB_T1_terminator
     primer_bind     complement(5593..5612)
                     /label=rADC160
     primer_bind     complement(5593..5612)
                     /label=pSKO1_seq7
                     /note="DEAMFCALM"
     primer_bind     5615..5636
                     /label=pBad18-Seq-Rev
     primer_bind     complement(5622..5643)
                     /label=JMR344
                     /note="pJMR2_cPCR_F_3"
     primer_bind     complement(5661..5678)
                     /label=rMP045
     primer_bind     5683..5703
                     /label=JMR181
                     /note="pBAD*_seqR2,pBAD seq reverse"
     primer_bind     5687..5706
                     /label=JMR78
                     /note="pBAD_cPCR_R,pBAD35 reverse sequencing"
     primer_bind     5702..5720
                     /label=RYL022
     primer_bind     5702..5720
                     /label=pTRC99A.seq.REV
                     /note="CRM"
     primer_bind     complement(5730..5756)
                     /label=pBAD18-fw
                     /note="DMP"
     primer_bind     complement(5730..5756)
                     /label=pBAD18-Fw
     primer_bind     complement(5732..5752)
                     /label=SF_MB1_5'
                     /note="DEAMFCALM"
     primer_bind     5747..5769
                     /label=JMR209
                     /note="pJT_cPCR Reverse"
     primer_bind     5757..5775
                     /label=RYL010
     primer_bind     5757..5775
                     /label=pMSB6_seqR_1
                     /note="MFCMPDEA"
     primer_bind     5762..5778
                     /label=MFC010
                     /note="MFC"
     primer_bind     complement(5763..5783)
                     /label=rMP432
     primer_bind     5766..5786
                     /label=JMR81
                     /note="pBAD*_seqR,pBAD35 sequence reverse"
     misc_feature    5775..5792
                     /label=pBAD_rev_primer
     misc_feature    5775..5792
                     /label=pTrcHis_rev_primer
     primer_bind     5775..5792
                     /label=*FuncBio* pBAD Reverse
                     /note="This is a free sequencing primer offered by 
                     Functional Biosciences"
     primer_bind     complement(5784..5806)
                     /label=rMP360
     primer_bind     5789..5813
                     /label=pNHL06
     primer_bind     complement(5789..5813)
                     /label=pNHL05
     primer_bind     complement(5792..5813)
                     /label=elongation24_seq5fwd
                     /note="MP"
     primer_bind     complement(5793..5813)
                     /label=rMP071
     primer_bind     complement(5795..5824)
                     /label=ptrc-GibFw
     primer_bind     complement(5801..5822)
                     /label=rMP200
     primer_bind     complement(5801..5820)
                     /label=JC8
                     /note="JCC"
     primer_bind     complement(5803..5827)
                     /label=pBAD18-GibFw
     primer_bind     complement(5804..5827)
                     /label=pBad18-Fw
     primer_bind     5804..5824
                     /label=rTS04
     primer_bind     complement(5804..5824)
                     /label=rTS01
     primer_bind     complement(5804..5822)
                     /label=rTBC0413
     primer_bind     complement(5804..5822)
                     /label=pCas9_reverse
     primer_bind     complement(5806..5828)
                     /label=pMP06-Gibs-Fw
     primer_bind     complement(5806..5823)
                     /label=pTRC_gib_F
                     /note="TY"
     primer_bind     complement(5807..5824)
                     /label=elongation24_fwd
                     /note="MP"
     primer_bind     5808..5828
                     /label=pBudE35_seqR
                     /note="JR"
     misc_feature    5823..5923
                     /label=Spacer Included in Qi et. al. 2013
                     /note="Spacer Included in Qi et. al. 2013 ""Repurposing 
                     CRISPR..."""
     primer_bind     5907..5923
                     /label=rCRM026
                     /note="pgRNA target seq R"
     misc_feature    5924..5964
                     /label=S. pyogenes terminator
                     /note="S. pyogenes terminator"
     primer_bind     complement(5959..5976)
                     /label=rMP306
     primer_bind     5959..5976
                     /label=rMP305
     misc_feature    5965..6006
                     /label=cas9 Handle
                     /note="cas9 Handle"
     primer_bind     complement(5972..6006)
                     /label=Ec-F
                     /note="ALM556"
     primer_bind     complement(5984..6026)
                     /label=rMP395
     primer_bind     complement(5984..6006)
                     /label=rTBC0339
     primer_bind     6007..6048
                     /label=rMP400
     misc_feature    6007..6026
                     /label=gRNA targeting region for pBR322 ori
                     /note="gRNA targeting region for pBR322 ori"
     primer_bind     6026..6048
                     /label=rTBC0340
     misc_feature    6026
                     /label=PLtetO1 TSP
                     /note="PLtetO1 TSP"
     primer_bind     6027..6061
                     /label=rMP280
     primer_bind     6027..6044
                     /label=rMP278
     misc_feature    6033..6038
                     /label=-10
                     /note="-10"
     misc_feature    6037..6055
                     /label=tet operator
                     /note="tet operator"
     primer_bind     6047..6106
                     /label=rMP162
     misc_feature    6047..6086
                     /label=rMP162 tail
                     /note="rMP162 tail"
     misc_feature    6056..6061
                     /label=-35
                     /note="-35"
     misc_feature    6062..6080
                     /label=tet operator
                     /note="tet operator"
     primer_bind     complement(6087..6107)
                     /label=rHS007
     primer_bind     6091..6111
                     /label=pMP-seq1
                     /note="MP"
     primer_bind     complement(6098..6130)
                     /label=p70RGbackbone-rev
                     /note="MP"
     primer_bind     complement(6107..6130)
                     /label=rMP361
     primer_bind     complement(6107..6130)
                     /label=pBAD24amp_rev
     primer_bind     6108..6131
                     /label=rHS004
     misc_feature    6137..6214
                     /label=lacIq region
     primer_bind     6137..6167
                     /label=rMP258
     primer_bind     complement(6137..6167)
                     /label=rMP257
     primer_bind     complement(6184..6205)
                     /label=ALM042 backbone Rev
                     /note="MB"
     misc_feature    6185..6258
                     /label=rMP168
                     /note="rMP168"
     primer_bind     complement(6185..6258)
                     /label=rMP168
     primer_bind     complement(6187..6208)
                     /label=WN26
     misc_feature    6187
                     /label=lacI TSP
                     /note="lacI TSP"
     primer_bind     complement(6194..6214)
                     /label=rMP161
     gene            6215..6838
                     /label=tetR
                     /note="tetR"
     primer_bind     6215..6241
                     /label=rMP159
     primer_bind     6247..6270
                     /label=tetR_R_cPCR
                     /note="65.4C"
     primer_bind     complement(6288..6307)
                     /label=GG317
                     /note="TetR seq sgRNA"
     primer_bind     complement(6309..6326)
                     /label=rMP177
     primer_bind     complement(6325..6348)
                     /label=WN24
     primer_bind     complement(6408..6431)
                     /label=JMR282
                     /note="pBbS2k-2791 cPCR forward"
     primer_bind     6465..6484
                     /label=rMP183
     primer_bind     complement(6485..6503)
                     /label=Nest TetR-seq_F
                     /note="59.7C"
     primer_bind     complement(6805..6824)
                     /label=rMP176
     primer_bind     complement(6815..6865)
                     /label=rMP160
     primer_bind     complement(6815..6838)
                     /label=TetR* Insert FWD
                     /note="EKZ"
     misc_feature    6838..6897
                     /label=rMP167
                     /note="rMP167"
     primer_bind     complement(6838..6897)
                     /label=rMP167
     misc_feature    6839..6865
                     /label=rMP160 overhang
                     /note="rMP160 overhang"
     primer_bind     6845..6892
                     /label=rADC068
     terminator      6849..6882
                     /label=arcA terminator (bidirectional)
                     /note="arcA terminator (bidirectional)"
     primer_bind     6866..6913
                     /label=rMP166
     misc_feature    6866..6892
                     /label=rMP166 overhang
                     /note="rMP166 overhang"
     primer_bind     6893..6916
                     /label=GibATW_pBT2-5'R
                     /note="MFC"
     primer_bind     6898..6918
                     /label=rTBC0412
     primer_bind     6898..6918
                     /label=pCas9_forward
     primer_bind     6910..6931
                     /label=Gib_pBT2TEV-Ra
                     /note="60C,60C"
     primer_bind     6911..6940
                     /label=Gib_pBT-Out2-R
                     /note="64.5C"
     primer_bind     6911..6940
                     /label=Gib_pBT-Out-R
                     /note="64.5C"
     primer_bind     6914..6937
                     /label=rMP192
     primer_bind     6917..6940
                     /label=Gib_a-pBT2-R
                     /note="61.4C"
     primer_bind     complement(6918..6944)
                     /label=rTBC0203
     primer_bind     complement(6918..6941)
                     /label=ATW_pBT1xx_R
                     /note="MFC"
     primer_bind     6921..6940
                     /label=rMP293
     primer_bind     complement(6924..6953)
                     /label=rMP194
     primer_bind     complement(6934..6959)
                     /label=*FuncBio* SR2
                     /note="This is a free sequencing primer offered by 
                     Functional Biosciences"
     primer_bind     6944..6967
                     /label=iG_pBT102_Fb
                     /note="62C"
     primer_bind     6946..6969
                     /label=ATW_pBT1xx_F
                     /note="MFC"
     primer_bind     complement(6949..6971)
                     /label=rTBC0146
     primer_bind     6962..6979
                     /label=rMP182
     primer_bind     6967..6993
                     /label=pBT1xx_T to C_R
                     /note="MFC"
     primer_bind     complement(6967..6993)
                     /label=pBT1xx_T to C_F
                     /note="MFC"
     terminator      6972..7003
                     /label=TonB terminator
                     /note="TonB terminator (in correct orientation if 
                     transcription is from left to right - this direction is 
                     somewhat more efficient than the reverse orientation, 
                     though the terminator is bidirectional)"
     primer_bind     complement(6986..7006)
                     /label=pBTseq2-F
                     /note="MFC"
     primer_bind     6990..7043
                     /label=rMP331
     primer_bind     complement(6997..7046)
                     /label=rMP323
     primer_bind     complement(6997..7016)
                     /label=rMP329
     primer_bind     7017..7035
                     /label=rMP320
     primer_bind     7019..7037
                     /label=JC185
     primer_bind     complement(7027..7043)
                     /label=A0061 BB F (2)
                     /note="ALM289"
     primer_bind     complement(7027..7043)
                     /label=A0061 BB F
                     /note="REC"
     primer_bind     complement(7029..7049)
                     /label=JMR458
                     /note="pMP11 Forward - Red gene deletion"
     CDS             complement(7054..7932)
                     /codon_start=1
                     /label=araC protein
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     7054..7081
                     /label=SF_Red_3'
                     /note="DEAMFCALM"
     primer_bind     7096..7113
                     /label=rMP417
     primer_bind     complement(7120..7140)
                     /label=rHS043
     primer_bind     complement(7125..7144)
                     /label=rMP325
     primer_bind     complement(7155..7177)
                     /label=pSKO PheS promoter seq
                     /note="ALM111"
     primer_bind     complement(7161..7179)
                     /label=rMP420
     primer_bind     complement(7237..7256)
                     /label=JMR343
                     /note="pJMR2_cPCR_R_2"
     primer_bind     7529..7546
                     /label=araBAD_seq_F
     primer_bind     complement(7601..7623)
                     /label=cPCR_AraC-F
                     /note="DEAMFCALM"
     primer_bind     complement(7604..7623)
                     /label=pSKO1_seq6
                     /note="DEAMFCALM"
     primer_bind     7680..7700
                     /label=araB-out-fwd
                     /note="MP"
     primer_bind     complement(7689..7708)
                     /label=Berkley SD R1
                     /note="EW (iGEM)"
     primer_bind     7718..7737
                     /label=Berkley SD F2
                     /note="EW (iGEM)"
     primer_bind     7872..7892
                     /label=WN28
     primer_bind     7878..7899
                     /label=araC_seq_F
                     /note="TY"
     primer_bind     7884..7905
                     /label=JMR248
                     /note="pBAD-PP_2791 cPCR forward"
     primer_bind     7969..7989
                     /label=rMP243
     primer_bind     7989..8009
                     /label=rMP011
     primer_bind     8001..8022
                     /label=rCRM036
                     /note="araBAD screening R"
     primer_bind     8007..8028
                     /label=pBad18-Seq-FW
     primer_bind     8055..8078
                     /label=pBAD33-seqfw
                     /note="DMP"
     primer_bind     8056..8077
                     /label=JMR77
                     /note="pBAD_cPCR_F,pBAD35 forward sequencing"
     primer_bind     8076..8097
                     /label=JMR218
                     /note="pBad35_JT_seq_1,""pBAD35 sequence primer, promoter 
                     region"""
     primer_bind     8076..8097
                     /label=JMR168
                     /note="pBad35seq_1,pBAD35 sequencing"
     primer_bind     8082..8101
                     /label=elongation24_seq1fwd
                     /note="MP"
     misc_feature    8083..8111
                     /label=Pc
                     /note="Pc"
     primer_bind     8094..8112
                     /label=rMP421
     primer_bind     8153..8173
                     /label=JMR180
                     /note="pBAD*_seqF,pBAD seq forward"
     primer_bind     8166..8185
                     /label=*FuncBio* pBAD Forward
                     /note="This is a free sequencing primer offered by 
                     Functional Biosciences"
     primer_bind     complement(8192..8211)
                     /label=elongation24_seq1rev
                     /note="MP"
     misc_feature    8208..8235
                     /label=ParaB
                     /note="ParaB"
     primer_bind     complement(8210..8241)
                     /label=pBAD18-Rv (2)
     primer_bind     complement(8210..8234)
                     /label=rNHL37
     primer_bind     8210..8234
                     /label=rNHL36
     primer_bind     8219..8240
                     /label=JMR138
                     /note="RBS_Test_Forward,91-94 RBS testing forward"
     primer_bind     complement(8223..8243)
                     /label=rMP433
     primer_bind     complement(8229..8251)
                     /label=pBADgibR
                     /note="TY"
     primer_bind     8229..8249
                     /label=mdtABCD_seq1
                     /note="RL"
     primer_bind     8230..8249
                     /label=mdtEF_seq1
                     /note="RL"
     primer_bind     complement(8238..8257)
                     /label=elongation24_rev
                     /note="MP"
     primer_bind     complement(8257..8286)
                     /label=rTBC0475
     primer_bind     8264..8307
                     /label=rTBC0610
     CDS             8287..9072
                     /codon_start=1
                     /label=bet protein
                     /note="recombination;261"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     primer_bind     complement(8586..8605)
                     /label=pSKO1_seq4
                     /note="DEAMFCALM"
     primer_bind     complement(9055..9095)
                     /label=rTBC0611
     primer_bind     complement(9055..9072)
                     /label=rTBC0973
     primer_bind     9073..9095
                     /label=rTBC0476
     primer_bind     9073..9094
                     /label=rTBC0204
     primer_bind     9074..9118
                     /label=JMR450
                     /note="RK2 ori Forward :: pMP11"
     primer_bind     complement(9074..9097)
                     /label=JMR453
                     /note="pMP11 Forward"
     primer_bind     9097..9118
                     /label=JMR454
                     /note="trfA F (error prone PCR)"
     primer_bind     9099..9125
                     /label=WN74
                     /note="id."
     CDS             complement(9159..10307)
                     /codon_start=1
                     /transl_table=11
                     /gene="trfA"
                     /product="replication protein"
                     /label=trfA
                     /db_xref="GI:525567860"
                     /protein_id="AGR55406.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALKNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     gene            complement(9159..10307)
                     /gene="trfA"
                     /label=trfA
     primer_bind     complement(9332..9354)
                     /label=WN84
                     /note="id. 224"
     primer_bind     9462..9482
                     /label=JMR230
                     /note="pRK415seq_7,""pRK415 sequence primer, trfA1-2"""
     primer_bind     9731..9747
                     /label=pSeq3
                     /note="pSEVA224 trfA partial sequencing"
     primer_bind     complement(10155..10175)
                     /label=Pseq1
                     /note="""pSEVA224 sequencing"
     primer_bind     complement(10404..10421)
                     /label=rHS091
     primer_bind     10502..10519
                     /label=rHS089
     primer_bind     10562..10582
                     /label=JMR456
                     /note="pBb(SEVA224)1k-GFP F"
     primer_bind     complement(10562..10582)
                     /label=JMR455
                     /note="trfA R (error prone PCR)"
     primer_bind     complement(10569..10589)
                     /label=JMR174
                     /note="pJoe96KOseq_1,pJOE 2796 KO gmR seq"
     rep_origin      10700..11231
                     /label=oriV
                     /note="incP origin of replication"
     primer_bind     complement(10851..10871)
                     /label=Pseq2
                     /note="""pSEVA224 sequencing"
     primer_bind     10979..10998
                     /label=WN81
                     /note="id. 224"
     primer_bind     complement(join(11299..11313,1..30))
                     /label=WN75
                     /note="id."
ORIGIN
        1 cctttgatct tttctacact agtgcttgga ttctcacctt ggtctgacag ttaccaatgc
       61 gaggggggga tccaccttcg ggagcgcctg aagcccgttc tggacgccct ggggccgttg
      121 aatcgggata tgcaggccaa ggccgccgcg atcatcaagg ccgtgggcga aaagctgctg
      181 acggaacagc gggaagtcca gcgccagaaa caggcccagc gccagcagga acgcgggcgc
      241 gcacatttcc ccgaaaagtg ccacctgcgg cgttgtgaca atttaccgaa caactccgcg
      301 gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca
      361 aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca
      421 ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg
      481 agattgatga gcgcggtggc aatgccctgc ctccggtgct ctccggagac tgcgagatca
      541 tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg
      601 ccaacaacca cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag
      661 ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca
      721 cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat
      781 gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc
      841 gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
      901 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata
      961 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
     1021 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt
     1081 caactgggtt cgtgccttca tccgtttcca cggtgtgcgt ccatgggcaa atattatacg
     1141 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc
     1201 ttccatgtcg gcagaatgct taatgaatta caacagtttt tatgcatgcg cagtccagtt
     1261 acgctggagt ctgaggctcg tcctgaatga tatctcattt atcatcatca tctttataat
     1321 caccgctgcc gtcacctcct agctgactca aatcaatgcg tgtttcataa agaccagtga
     1381 tggattgatg gataagagtg gcatctaaaa cttcttttgt agacgtatat cgtttacgat
     1441 caattgttgt atcaaaatat ttaaaagcag cgggagctcc aagattcgtc aacgtaaata
     1501 aatgaataat attttctgct tgttcacgta ttggtttgtc tctatgtttg ttatatgcac
     1561 taagaacttt atctaaattg gcatctgcta aaataacacg cttagaaaat tcactgattt
     1621 gctcaataat ctcatctaaa taatgcttat gctgctccac aaacaattgt ttttgttcgt
     1681 tatcttctgg actacccttc aacttttcat aatgactagc taaatataaa aaattcacat
     1741 atttgcttgg cagagccagc tcatttcctt tttgtaattc tccggcacta gccagcatcc
     1801 gtttacgacc gttttctaac tcaaaaagac tatatttagg tagtttaatg attaagtctt
     1861 ttttaacttc cttatatcct ttagcttcta aaaagtcaat cggatttttt tcaaaggaac
     1921 ttctttccat aattgtgatc cctagtaact ctttaacgga ttttaacttc ttcgatttcc
     1981 ctttttccac cttagcaacc actaggactg aataagctac cgttggacta tcaaaaccac
     2041 catatttttt tggatcccag tcttttttac gagcaataag cttgtccgaa tttctttttg
     2101 gtaaaattga ctccttggag aatccgcctg tctgtacttc tgttttcttg acaatattga
     2161 cttggggcat ggacaatact ttgcgcactg tggcaaaatc tcgcccttta tcccagacaa
     2221 tttctccagt ttccccatta gtttcgatta gagggcgttt gcgaatctct ccatttgcaa
     2281 gtgtaatttc tgttttgaag aagttcatga tattagagta aaagaaatat tttgcggttg
     2341 ctttgcctat ttcttgctca gacttagcaa tcattttacg aacatcataa actttataat
     2401 caccatagac aaactccgat tcaagttttg gatatttctt aatcaaagca gttccaacga
     2461 cggcatttag atacgcatca tgggcatgat ggtaattgtt aatctcacgt actttataga
     2521 attggaaatc ttttcggaag tcagaaacta atttagattt taaggtaatc actttaacct
     2581 ctcgaataag tttatcattt tcatcgtatt tagtattcat gcgactatcc aaaatttgtg
     2641 ccacatgctt agtgatttgg cgagtttcaa ccaattggcg tttgataaaa ccagctttat
     2701 caagttcact caaacctcca cgttcagctt tcgttaaatt atcaaactta cgttgagtga
     2761 ttaacttggc gtttagaagt tgtctccaat agtttttcat ctttttgact acttcttcac
     2821 ttggaacgtt atccgattta ccacgatttt tatcagaacg cgttaagacc ttattgtcta
     2881 ttgaatcgtc tttaaggaaa ctttgtggaa caatgtgatc gacatcataa tcacttaaac
     2941 gattaatatc taattcttgg tccacataca tgtctcttcc attttggaga taatagagat
     3001 agagcttttc attttgcaat tgagtatttt caacaggatg ctctttaaga atctgacttc
     3061 ctaattcttt gataccttct tcgattcgtt tcatacgctc tcgcgaattt ttctggccct
     3121 tttgagttgt ctgattttca cgtgccattt caataacgat attttctggc ttatgccgcc
     3181 ccattacttt gaccaattca tcaacaactt ttacagtctg taaaatacct tttttaatag
     3241 cagggctacc agctaaattt gcaatatgtt catgtaaact atcgccttgt ccagacactt
     3301 gtgctttttg aatgtcttct ttaaatgtca aactatcatc atggatcagc tgcataaaat
     3361 tgcgattggc aaaaccatct gatttcaaaa aatctaatat tgttttgcca gattgcttat
     3421 ccctaatacc attaatcaat tttcgagaca aacgtcccca accagtataa cggcgacgtt
     3481 taagctgttt catcacctta tcatcaaaga ggtgagcata tgttttaagt ctttcctcaa
     3541 tcatctccct atcttcaaat aaggtcaatg ttaaaacaat atcctctaag atatcttcat
     3601 tttcttcatt atccaaaaaa tctttatctt taataatttt tagcaaatca tggtaggtac
     3661 ctaatgaagc attaaatcta tcttcaactc ctgaaatttc aacactatca aaacattcta
     3721 tttttttgaa ataatcttct tttaattgct taacggttac ttttcgattt gttttgaaga
     3781 gtaaatcaac aatggctttc ttctgttcac ctgaaagaaa tgctggtttt cgcattcctt
     3841 cagtaacata tttgaccttt gtcaattcgt tataaaccgt aaaatactca taaagcaaac
     3901 tatgttttgg tagtactttt tcatttggaa gatttttatc aaagtttgtc atgcgttcaa
     3961 taaatgattg agctgaagca cctttatcga caacttcttc aaaattccat ggggtaattg
     4021 tttcttcaga cttccgagtc atccatgcaa aacgactatt gccacgcgcc aatggaccaa
     4081 cataataagg aattcgaaaa gtcaagattt tttcaatctt ctcacgattg tcttttaaaa
     4141 atggataaaa gtcttcttgt cttctcaaaa tagcatgcag ctcacccaag tgaatttgat
     4201 ggggaataga gccgttgtca aaggtccgtt gcttgcgcag caaatcttca cgatttagtt
     4261 tcaccaataa ttcctcagta ccatccattt tttctaaaat tggtttgata aatttataaa
     4321 attcttcttg gctagctccc ccatcaatat aacctgcata tccgtttttt gattgatcaa
     4381 aaaagatttc tttatacttt tctggaagtt gttgtcgaac taaagctttt aaaagagtca
     4441 agtcttgatg atgttcatcg tagcgtttaa tcattgaagc tgatagggga gccttagtta
     4501 tttcagtatt tactcttagg atatctgaaa gtaaaatagc atctgataaa ttcttagctg
     4561 ccaaaaacaa atcagcatat tgatctccaa tttgcgccaa taaattatct aaatcatcat
     4621 cgtaagtatc ttttgaaagc tgtaatttag catcttctgc caaatcaaaa tttgatttaa
     4681 aattaggggt caaacccaat gacaaagcaa tgagattccc aaataagcca tttttcttct
     4741 caccggggag ctgagcaatg agattttcta atcgtcttga tttactcaat cgtgcagaaa
     4801 gaatcgcttt agcatctact ccacttgcgt taatagggtt ttcttcaaat aattgattgt
     4861 aggtttgtac caactggata aatagtttgt ccacatcact attatcagga tttaaatctc
     4921 cctcaatcaa aaaatgacca cgaaacttaa tcatatgcgc taaggccaaa tagattaagc
     4981 gcaaatccgc tttatcagta gaatctacca atttttttcg cagatgatag atagttggat
     5041 atttctcatg ataagcaact tcatctacta tatttccaaa aataggatga cgttcatgct
     5101 tcttgtcttc ttccaccaaa aaagactctt caagtcgatg aaagaaacta tcatctactt
     5161 tcgccatctc atttgaaaaa atctcctgta gataacaaat acgattcttc cgacgtgtat
     5221 accttctacg agctgtccgt ttgagacgag tcgcttccgc tgtctctcca ctgtcaaata
     5281 aaagagcccc tataagattt tttttgatac tgtggcggtc tgtatttccc agaaccttga
     5341 actttttaga cggaacctta tattcatcag tgatcaccgc ccatccgacg ctatttgtgc
     5401 cgatatctaa gcctattgag tatttcttat ccattgattg tacctcctgg tcagtgcgtc
     5461 ctgctgatag ctagcatagt acctaggact gagctagccg taaactctag aagcggccgc
     5521 gaattcaact gcagtctaga ctcgagtaag gatccagttc accgacaaac aacagataaa
     5581 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     5641 ctctcgcatg gggagacccc acactaccat cggcgctacg gcgtttcact tctgagttcg
     5701 gcatggggtc aggtgggacc accgcgctac tgccgccagg caaattctgt tttatcagac
     5761 cgcttctgcg ttctgattta atctgtatca ggctgaaaat cttctctcat ccgccaaaac
     5821 agccaagctg gagaccgttt aaactcaatg atgatgatga tgatggtcga cggcgctatt
     5881 cagatcctct tctgagatga gtttttgttc gggcccaagc ttcaaaaaaa gcaccgactc
     5941 ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tttctagctc
     6001 taaaacggaa gctccctcgt gcgctcgtgc tcagtatctc tatcactgat agggatgtca
     6061 atctctatca ctgataggga ctcgagctat gctactccgt caagccgtca attgtctgat
     6121 tcgttaccaa ctcgaggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata
     6181 gcgcccggaa gagagtcaat tcagggtggt gaatatgtct agattagata aaagtaaagt
     6241 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa
     6301 actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg
     6361 ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc acttttgccc
     6421 tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc
     6481 tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca
     6541 gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa
     6601 tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca
     6661 agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt
     6721 attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg
     6781 ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcttaata
     6841 ccaccgtcaa aaaaaacggc gctttttagc gccgttttta tttttcaacc ttaagcttga
     6901 attcgttgac gaattctcta gatatcgctc aatactgacc atttaaatca tacctgacct
     6961 ccatagcaga aagtcaaaaa cctccgaccg gaggcttttg acttgagggg gatccggcac
     7021 atttccccga aaagtgccac ctgcatcgat ttattatgac aacttgacgg ctacatcatt
     7081 cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa
     7141 tacccgcgag aagtagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg
     7201 catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct
     7261 taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca
     7321 aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta
     7381 ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc
     7441 catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc
     7501 ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc
     7561 ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa gccattcatg
     7621 ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg
     7681 acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac
     7741 aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata
     7801 acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg
     7861 cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg
     7921 cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg
     7981 catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca aaccggtaac
     8041 cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa
     8101 caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact
     8161 ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac gctttttatc
     8221 gcaactctct actgtttctc catacccgtt tttttgggaa ttcgagctct aaggaggtta
     8281 taaaaaatga gtactgcact cgcaacgctg gctgggaagc tggctgaacg tgtcggcatg
     8341 gattctgtcg acccacagga actgatcacc actcttcgcc agacggcatt taaaggtgat
     8401 gccagcgatg cgcagttcat cgcattactg atcgttgcca accagtacgg ccttaatccg
     8461 tggacgaaag aaatttacgc ctttcctgat aagcagaatg gcatcgttcc ggtggtgggc
     8521 gttgatggct ggtcccgcat catcaatgaa aaccagcagt ttgatggcat ggactttgag
     8581 caggacaatg aatcctgtac atgccggatt taccgcaagg accgtaatca tccgatctgc
     8641 gttaccgaat ggatggatga atgccgccgc gaaccattca aaactcgcga aggcagagaa
     8701 atcacggggc cgtggcagtc gcatcccaaa cggatgttac gtcataaagc catgattcag
     8761 tgtgcccgtc tggccttcgg atttgctggt atctatgaca aggatgaagc cgagcgcatt
     8821 gtcgaaaata ctgcatacac tgcagaacgt cagccggaac gcgacatcac tccggttaac
     8881 gatgaaacca tgcaggagat taacactctg ctgatcgccc tggataaaac atgggatgac
     8941 gacttattgc cgctctgttc ccagatattt cgccgcgaca ttcgtgcatc gtcagaactg
     9001 acacaggccg aagcagtaaa agctcttgga ttcctgaaac agaaagccgc agagcagaag
     9061 gtggcagcat gatccatggg tatggacagt tttcccttta tacctagggc gatgcaggtg
     9121 gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca atgcaccagg
     9181 tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg
     9241 ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat gaggcggaag
     9301 gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa catccgtcgg
     9361 gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg gtcgccagca
     9421 aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt cttgccacgg
     9481 tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac gcggtcggac
     9541 gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac
     9601 cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc
     9661 tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag ttcgtcatcg
     9721 tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg
     9781 accttgtttt tcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag
     9841 cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag
     9901 gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggct
     9961 tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt
    10021 cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc gagacgacgc
    10081 gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg cacgctgtcg
    10141 cgctcgatct tggccgtagc ttgctggact atcgagccga cggactggaa ggtttcgcgg
    10201 ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg
    10261 tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca ccctccttgc
    10321 gggattgccc cggaattaat tccccggatc gatccgtcga tcttgatccc ctgcgccatc
    10381 agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag
    10441 agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct
    10501 atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg
    10561 tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt
    10621 tctacgtggc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg
    10681 gggatgggag gcccgcgtta gcgggccggg agggttcgag aagggggggc accccccttc
    10741 ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt
    10801 ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg
    10861 caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat
    10921 ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg
    10981 cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg
    11041 aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc
    11101 ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa
    11161 gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa
    11221 cgccggcggc cctacatggc tctgctgtag tgagtgggtt gcgctccggc agcggtcctg
    11281 atcccccgca gaaaaaaagg atctcaagaa gat
//
