LOCUS RiboGcamp8s-GS-linker_( 6575 bp ds-DNA circular 17-JUN-2021 DEFINITION . KEYWORDS "accession:addgene_158777_313160" SOURCE synthetic DNA construct ORGANISM synthetic DNA construct FEATURES Location/Qualifiers promoter 1..448 /label="hSyn" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 482..1747 /label="jGCaMP8s" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 CDS 482..2479 /label="Translation 482-2479" misc_feature 1748..1828 /label="Linker" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 CDS 1829..2479 /label="RL10" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 2520..3108 /label="WPRE" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer_bind complement(2573..2593) /label="WPRE-R" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd CDS complement(2991..3002) /label="Factor Xa site" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 polyA_signal 3140..3616 /label="hGH poly(A)" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer_bind complement(3339..3358) /label="hGH-PA-R" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 repeat_region 3656..3785 /label="AAV2 ITR" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 rep_origin 3871..4326 /label="f1 ori" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer_bind complement(3958..3977) /label="F1ori-R" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer_bind 4168..4189 /label="F1ori-F" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer_bind complement(4343..4362) /label="pRS-marker" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer_bind 4462..4484 /label="pGEX 3'" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer_bind complement(4522..4540) /label="pBRforEco" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e promoter 4608..4712 /label="AmpR promoter" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e CDS 4713..5573 /label="AmpR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer_bind complement(4931..4950) /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac rep_origin 5744..6332 /label="ori" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer_bind 6233..6252 /label="pBR322ori-F" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 repeat_region 6394..6523 /label="AAV2 ITR" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 ORIGIN 1 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga 61 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga 121 gagggggagg ggaaacagga tgcggcgagg ctcgtgcgca ctgccagctt cagcaccgcg 181 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc 241 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg 301 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg 361 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg 421 aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg ctagagGATC CACCCGCCAC 481 CATGCATCAT CACCATCATC ACACGCGTCG CAAGAAGACC TTCAAGGAGG TGGCCACCGC 541 CGTGAAGATC ATCGCCATGC TGATGGGCCT GAAGATCAAC GTCTATATCA AGGCCGACAA 601 GCAGAAGAAC GGCATCAAGG CGAACTTCCA CATCCGCCAC AACATCGAGG ACGGCGGCGT 661 GCAGCTCGCC TACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC 721 CGACAACCAC TACCTGAGCG TGGAGTCCAA ACTTTCGAAA GACCCCAACG AGAAGCGCGA 781 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT 841 GTACAAGGGC GGTACCGGAG GGAGCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT 901 GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG 961 CGAGGGTGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG 1021 CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT 1081 CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG 1141 CTACATCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA 1201 GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA 1261 GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC CTGCCGGACC AACTGACTGA 1321 AGAGCAGATC GCAGAATACA AAGAGGCTTT CTCCCTATTT GACAAGGACG GGGATGGGAC 1381 AATAACAACC AAGGAGCTGG GGACGGTGAT GCGGTCTCTC GGACACAACC CCACAGAAGC 1441 AGAGCTGCAG GACATGATCA ATGAAGTAGA TGCCGACGGT GACGGCACAA TCGACTTCCC 1501 TGAGTTCCTG ACAATGATGG CAAGAAAAAT GAAATACAGG GACACGGAAG AAGAAATTAG 1561 AGAAGCGTTC GGTGTGTTTG ATAAGGATGG CAATGGCTAC ATCAGTGCAG CAGAGCTTCG 1621 CCACGTGATG ACAAACCTTG GAGAGAAGTT aacagatgaa gaggttgatg aaatgatcag 1681 ggaagcagac atcgatgggg atggtcaggt aaactacgaa gagtttgtac aaatgatgac 1741 agcgaagGGG GGCAGCGGTG GGTCTGGAGG GACTGGGGGT AGCGGTGGTA GCGGTGGGAC 1801 AGGGGGAAGC GGCGGTAGTG GTGGAACAag cagcaaagtc tcacgcgaca ccctgtacga 1861 ggcggtgcgg gaagtcctgc acgggaacca gcgcaagcgc cgcaagtttc tggagacggt 1921 ggagctgcag atcagcctga agaactacga ccctcagaag gacaaacgtt tctcgggcac 1981 cgtcaggctc aagtccaccc cacgccccaa gttctcggtg tgcgttctgg gggaccagca 2041 gcactgtgat gaagccaagg ccgtggatat cccccacatg gacatcgagg cgctcaagaa 2101 acttaacaaa aacaagaagt tggtcaagaa gctggctaag aagtacgatg cctttttggc 2161 ctctgagtct ctgattaagc agatcccacg tatcctgggc ccaggcctaa acaaggctgg 2221 caagttcccc tccctgctga cacacaatga aaacatggtg gccaaagtgg atgaggtgaa 2281 atcgacaatc aagttccaga tgaagaaggt gctgtgtttg gccgtcgctg ttggccacgt 2341 gaagatgacc gatgatgagc tagtctacaa cattcatctg gctgtcaatt tcttggtgtc 2401 cttgcttaag aaaaactggc aaaacgtgcg ggctctgtac atcaagagca ccatgggcaa 2461 gccccagcgt ctgtattagg gcgcgcccct gcagggaatt cgatatcaag cttatcgata 2521 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 2581 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 2641 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 2701 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 2761 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 2821 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 2881 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 2941 cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 3001 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 3061 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag 3121 cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc 3181 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat 3241 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa 3301 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag 3361 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct 3421 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt 3481 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc 3541 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc 3601 tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa 3661 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg 3721 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg 3781 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt 3841 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg 3901 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc 3961 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 4021 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 4081 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 4141 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 4201 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt 4261 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta 4321 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc 4381 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 4441 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 4501 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 4561 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 4621 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 4681 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 4741 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 4801 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 4861 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 4921 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 4981 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 5041 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 5101 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 5161 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 5221 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 5281 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 5341 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 5401 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 5461 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 5521 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 5581 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 5641 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 5701 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 5761 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 5821 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 5881 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 5941 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 6001 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 6061 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 6121 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 6181 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 6241 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 6301 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 6361 tcctggcctt ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc 6421 tcactgaggc cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga 6481 gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg cacgcgttta 6541 attaagtgtc tagactgcag agggccctgc gtatg //