LOCUS       pAAV-hSyn-Soma-jGCaMP8m 6181 bp ds-DNA     circular     24-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      44..632
                     /label="ori"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     533..552
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     repeat_region   694..823
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        876..1323
                     /label="hSyn promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1357..2622
                     /label="GCaMP8m"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             1357..2976
                     /label="Translation 1357-2976"
     CDS             2629..2709
                     /label="Linker"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2710..2976
                     /label="EE-RR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3001..3589
                     /label="WPRE"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(3054..3074)
                     /label="WPRE-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(3472..3483)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     polyA_signal    3621..4097
                     /label="hGH poly(A)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(3820..3839)
                     /label="hGH-PA-R"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     repeat_region   4137..4266
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      4352..4807
                     /label="f1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     complement(4439..4458)
                     /label="F1ori-R"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     4649..4670
                     /label="F1ori-F"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(4824..4843)
                     /label="pRS-marker"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     4943..4965
                     /label="pGEX 3'"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(5003..5021)
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        5089..5193
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             5194..6054
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(5412..5431)
                     /label="Amp-R"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
       61 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
      121 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
      181 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
      241 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
      301 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
      361 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      421 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      481 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      541 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
      601 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
      661 tcctggcctt ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc
      721 tcactgaggc cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
      781 gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg cacgcgttta
      841 attaagtgtc tagactgcag agggccctgc gtatgagtgc aagtgggttt taggaccagg
      901 atgaggcggg gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca
      961 acccccattc cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg
     1021 cgaggctcgt gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc
     1081 ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg
     1141 caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg
     1201 caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc
     1261 gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg
     1321 cagtcgagaa accggctaga gGATCCACCC GCCACCATGC ATCATCACCA TCATCACACG
     1381 CGTCGCAAGA AGACCTTCAA GGAGGTGGCC ACCGCCGTGA AGATCATCGG CCGCCTGATG
     1441 GGCCTGAAGA TCAACGTCTA TATCAAGGCC GACAAGCAGA AGAACGGCAT CAAGGCGAAC
     1501 TTCCACATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCTACCA CTACCAGCAG
     1561 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCGTGGAG
     1621 TCCAAACTTT CGAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
     1681 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGGGCGGTAC CGGAGGGAGC
     1741 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
     1801 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GTGAGGGCGA TGCCACCTAC
     1861 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
     1921 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
     1981 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACA TCCAGGAGCG CACCATCTTC
     2041 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
     2101 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
     2161 AAGCTGGAGT ACAACCTGCC GGACCAACTG ACTGAAGAGC AGATCGCAGA ATACAAAGAG
     2221 GCTTTCTCCC TATTTGACAA GGACGGGGAT GGGACAATAA CAACCAAGGA GCTGGGGACG
     2281 GTGATGCGGT CTCTgGGgCA GAACCCCACA GAAGCAGAGC TGCAGGACAT GATCAATGAA
     2341 GTAGATGCCG ACGGTGACGG CACAATCGAC TTCCCTGAGT TCCTGACAAT GATGGCAAGA
     2401 AAAATGAAAT ACAGGGACAC GGAAGAAGAA ATTAGAGAAG CGTTCGGTGT GTTTGATAAG
     2461 GATGGCAATG GCTACATCAG TGCAGCAGAG CTTCGCCACG TGATGACAAA CCTTGGAGAG
     2521 AAGTTaacag atgaagaggt tgatgaaatg atcagggaag cagacatcga tggggatggt
     2581 caggtaaact acgaagagtt tgtacaaatg atgacagcga agGAGCTCgg gggcagcggt
     2641 gggtctggag ggactggggg tagcggtggt agcggtggga cagggggaag cggcggtagc
     2701 ggtgggacac tggaaatcga ggccgctttc ctggagcagg agaacaccgc cctggagaca
     2761 gaggtggctg agctggagca ggaggtgcag cggctggaga acatcgtgtc tcagtacgag
     2821 accagatacg gaccactggg atccctggag atccgggccg cttttctgag gagacggaac
     2881 accgccctga ggacaagggt ggctgagctg aggcagagag tgcagcggct gcgcaacatc
     2941 gtgagccagt acgagacacg ctacggacct ctgtaagaat tcgatatcaa gcttatcgat
     3001 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3061 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3121 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     3181 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     3241 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     3301 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     3361 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     3421 gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     3481 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3541 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga
     3601 gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg
     3661 ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca
     3721 ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca
     3781 aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga
     3841 gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc
     3901 tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt
     3961 tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat
     4021 ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg
     4081 ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga
     4141 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     4201 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     4261 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4321 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     4381 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
     4441 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4501 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4561 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4621 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4681 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
     4741 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4801 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4861 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4921 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4981 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5041 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5101 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5161 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5221 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5281 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5341 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5401 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5461 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5521 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5581 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5641 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5701 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5761 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5821 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5881 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5941 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6001 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6061 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6121 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6181 t
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