LOCUS       pAAV-hSyn-Soma-jGCaMP8f 6182 bp ds-DNA     circular     24-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      44..632
                     /label="ori"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     533..552
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     repeat_region   694..823
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        876..1323
                     /label="hSyn promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1357..2623
                     /label="GCamp8f"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             2630..2710
                     /label="Linker"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             2630..2977
                     /label="Translation 2630-2977"
     misc_feature    2711..2977
                     /label="EE-RR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3002..3590
                     /label="WPRE"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(3055..3075)
                     /label="WPRE-R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(3473..3484)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     polyA_signal    3622..4098
                     /label="hGH poly(A)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(3821..3840)
                     /label="hGH-PA-R"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     repeat_region   4138..4267
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      4353..4808
                     /label="f1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     complement(4440..4459)
                     /label="F1ori-R"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     4650..4671
                     /label="F1ori-F"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(4825..4844)
                     /label="pRS-marker"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     4944..4966
                     /label="pGEX 3'"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(5004..5022)
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        5090..5194
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             5195..6055
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(5413..5432)
                     /label="Amp-R"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
       61 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
      121 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
      181 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
      241 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
      301 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
      361 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      421 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      481 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      541 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
      601 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
      661 tcctggcctt ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc
      721 tcactgaggc cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
      781 gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg cacgcgttta
      841 attaagtgtc tagactgcag agggccctgc gtatgagtgc aagtgggttt taggaccagg
      901 atgaggcggg gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca
      961 acccccattc cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg
     1021 cgaggctcgt gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc
     1081 ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg
     1141 caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg
     1201 caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc
     1261 gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg
     1321 cagtcgagaa accggctaga gGATCCACCC GCCACCATGC ATCATCACCA TCATCACACG
     1381 CGTCGCAAGA AGACCTTCAA GGAGGTGGCC ACCGCCGTGA AGATCATCGC CCGCCTGATG
     1441 GGCCTGAAGA TCAACGTCTA TATCAAGGCC GACAAGCAGA AGAACGGCAT CAAGGCGAAC
     1501 TTCCACATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCTACCA CTACCAGCAG
     1561 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCGTGGAG
     1621 TCCAAACTTT CGAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
     1681 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGGGCGGTAC CGGAGGGAGC
     1741 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
     1801 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GTGAGGGCGA TGCCACCTAC
     1861 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
     1921 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
     1981 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACA TCCAGGAGCG CACCATCTTC
     2041 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
     2101 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
     2161 AAGCTGGAGT ACAACCTGCC GGACCAACTG ACTGAAGAGC AGATCGCAGA ATTTAAAGAG
     2221 GCTTTCTCCC TATTTGACAA GGACGGGGAT GGGACAATAA CAACCAAGGA GCTGGGGACG
     2281 GTGATGCGGT CTCTCGGACT GAACCCCACA GAAGCAGAGC TGCAGGACAT GATCAATGAA
     2341 GTAGATGCCG ACGGTGACGG CACAATCGAC TTCCCTGAGT TCCTGACAAT GATGGCAAGA
     2401 AAAATGAAAT ACAGGGACAC GGAAGAAGAA ATTAGAGAAG CGTTCGGTGT GTTTGATAAG
     2461 GATGGCAATG GCTACATCAG TGCAGCAGAG CTTCGCCACG TGATGACAAA CCTTGGAGAG
     2521 AAGTTaacag atgaagaggt tgatgaaatg atcagggaag cagacatcga tggggatggt
     2581 caggtaaact acgaagagtt tgtacaaatg atgacagcga agtGAGCTCg ggggcagcgg
     2641 tgggtctgga gggactgggg gtagcggtgg tagcggtggg acagggggaa gcggcggtag
     2701 cggtgggaca ctggaaatcg aggccgcttt cctggagcag gagaacaccg ccctggagac
     2761 agaggtggct gagctggagc aggaggtgca gcggctggag aacatcgtgt ctcagtacga
     2821 gaccagatac ggaccactgg gatccctgga gatccgggcc gcttttctga ggagacggaa
     2881 caccgccctg aggacaaggg tggctgagct gaggcagaga gtgcagcggc tgcgcaacat
     2941 cgtgagccag tacgagacac gctacggacc tctgtaagaa ttcgatatca agcttatcga
     3001 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     3061 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3121 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     3181 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     3241 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3301 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3361 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     3421 cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3481 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3541 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     3601 agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     3661 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     3721 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     3781 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     3841 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     3901 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     3961 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     4021 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     4081 gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg
     4141 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     4201 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     4261 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4321 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     4381 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     4441 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4501 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4561 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4621 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4681 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     4741 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4801 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4861 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4921 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4981 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5041 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5101 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5161 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5221 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5281 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5341 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5401 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5461 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5521 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5581 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5641 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5701 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5761 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5821 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5881 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5941 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6001 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6061 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6121 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6181 gt
//