LOCUS       pAAV-hSyn-JRex-GECO     5946 bp ds-DNA     circular     24-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      699..1287
                     /label="ori"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     1188..1207
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     repeat_region   1349..1478
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        1689..2136
                     /label="hSyn promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          2114..2133
                     /label="131_pAAV_hSyn_eGFPSeq1"
                     /note="sequence: TCGTGTCGTGCCTGAGAGCG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          2114..2133
                     /label="136_pAAV_hSyn_DIO_hM4D(Gi)_mCherry_F"
                     /note="sequence: tcgtgtcgtgcctgagagcg"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          2140..2167
                     /label="JRex-GECO-FWD-SpeI"
                     /note="sequence: gctgacactagttccatggtcgactcttcacgtc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2144..3403
                     /label="From pcDNA-CMV-JRex-GECO"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2149..3402
                     /label="jRex-geco"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             2149..3402
                     /label="Translation 2149-3402"
     primer          complement(2209..2230)
                     /label="637_JRex-GECO-rev-seq"
                     /note="sequence: aaacccaacgtgagctcagccg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3427..4015
                     /label="WPRE"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(3480..3500)
                     /label="WPRE-R"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(3898..3909)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     polyA_signal    4035..4511
                     /label="hGH poly(A) signal"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(4234..4253)
                     /label="hGH-PA-R"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     repeat_region   4557..4686
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      4772..5227
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(4859..4878)
                     /label="F1ori-R"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     5069..5090
                     /label="F1ori-F"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(5244..5263)
                     /label="pRS-marker"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     5363..5385
                     /label="pGEX 3'"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(5423..5441)
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        5509..5613
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             join(5614..5946,1..528)
                     /label="AmpR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(5832..5851)
                     /label="Amp-R"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
       61 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
      121 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
      181 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
      241 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
      301 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
      361 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
      421 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
      481 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
      541 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
      601 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
      661 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
      721 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      781 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      841 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
      901 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
      961 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1021 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1081 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1141 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1201 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1261 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1321 gccttttgct ggccttttgc tcacatgtcc tgcaggcagc tgcgcgctcg ctcgctcact
     1381 gaggccgccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg
     1441 cagagaggga gtggccaact ccatcactag gggttcctgc ggccgcacgc gttgcaaaga
     1501 tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa
     1561 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     1621 gagaagttgg ggggaggggt cggcagcaaa tggttaatta atctagactg cagagggccc
     1681 tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg tgcctacctg
     1741 acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat tgcgcatccc
     1801 ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact gccagcttca
     1861 gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg cctcagcact
     1921 gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg ccaccttggt
     1981 cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga gatagggggg
     2041 cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg
     2101 ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga gaggatccat ggtcgactct
     2161 tcacgtcgta agtggaataa ggcaggtcac gcagtcagag ctataggtcg gctgagctca
     2221 cgttgggttt ccgagtggat gtaccccgag gacggcgccc tgaagagcgt gatcaaggag
     2281 gggttgaggc tgaaggacgg cggccactac gccgccgagg tcaggaccac ctacaaggcc
     2341 aaaaagcccg tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga catcgtgtcc
     2401 cacaacgagg actacaccat cgtggaacag tgcgaacgcg ccgagggccg ccaccccacc
     2461 ggcggcatgg tcgggctgta caagggaggt acaggcggga gtctggtgag caagggcgag
     2521 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     2581 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgaggccttt
     2641 cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     2701 tcccttcagt tcatgtacgg ctccaaggcc tacattaagc acccagccga catccccgac
     2761 tacttcaagc tgtccttccc cgagggcttc aggtgggagc gcgtgatgat cttcgaggac
     2821 ggcggcatta ttcacgttaa ccaggactcc tccctgcagg acggcgtatt catctacaag
     2881 gtgaagctgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     2941 ggctgggagc ctacgcgtga cgatctgact gaagagcaga tcgcagagtt taaagaggct
     3001 ttctccctat ttgacaagga cggggatggg acgataacaa ccaaggagct ggggacggtg
     3061 ttccggtctc tggggcagaa ccccacagaa gcagagctgc aggacatgat caatgaagta
     3121 gatgccgacg gtgacggcac attcgacttc cctgagttcc tgacgatgat ggcaaggaaa
     3181 atgaatgact cagacagtga agaggaaatt agagaagcgt tccgcgtgtt tgataaggac
     3241 ggcaatggct acatcggcgc agcagagctt cgccacgtga tgacagacct tggagagaag
     3301 ttaacagatg aggaggttga tgaaatgatc agggtagcag acatcgatgg ggatggtcag
     3361 gtaaactacg aagagtttgt acaaatgatg acagcgaagt aggaattccg tacaaagctt
     3421 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3481 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3541 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3601 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3661 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3721 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3781 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     3841 ctgctcgcct atgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3901 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3961 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     4021 taccggctcg agacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
     4081 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
     4141 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
     4201 aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
     4261 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
     4321 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
     4381 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
     4441 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
     4501 tccctgtcct tctcgagctg attttgtagg taaccacgtg cggaccgagc ggccgcagga
     4561 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     4621 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     4681 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4741 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     4801 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
     4861 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4921 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4981 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5041 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5101 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
     5161 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     5221 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     5281 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     5341 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     5401 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5461 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5521 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5581 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5641 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5701 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5761 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5821 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5881 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5941 catctt
//