LOCUS       p414GPD-3xFLAG-N        7720 bp    DNA     circular UNA 27-AUG-2019
DEFINITION  an expression clone created from p414GPD-3xFLAG-GW and pDONR221 -
            NOP4_g with attB sites Entry clone (Circularized).
ACCESSION   urn.local...r4-aout0lf
VERSION     urn.local...r4-aout0lf
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          join(1..>3557,<5553..7720)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     complement(10..28)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /label="pBRforEco"
     misc_feature    70..573
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
                     /label="CEN/ARS"
     promoter        610..714
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             715..1575
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     primer_bind     complement(932..952)
                     /note="Ampicillin resistance gene, reverse primer"
                     /Sequence="ATAATACCGCGCCACATAGCA"
                     /Hairpin_Tm="None"
                     /Tm="60.4"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="Amp-R"
     primer_bind     933..970
                     /Mismatches=0
                     /Sequence="GCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGC"
                     /Mismatches=0
                     /annotation_group="a1be17b2-7460-45b2-aff0-4752cb10019b"
                     /label="NopdE5_GbF1"
     primer_bind     complement(934..952)
                     /Mismatches=0
                     /Mismatches=0
                     /%GC="47.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="54.9"
                     /annotation_group="15398771995018193d9dde13-319a-4109-964d
                     -f0cfa9fca0a9"
                     /Sequence="ATAATACCGCGCCACATAG"
                     /created_by="primer3"
                     /label="NopdE5_GbR2"
     primer_bind     953..969
                     /Mismatches=0
                     /Mismatches=0
                     /%GC="70.6"
                     /Hairpin_Tm="53.5"
                     /Self_Dimer_Tm="2.2"
                     /Tm="60.3"
                     /annotation_group="1532593290858721af1f80d8-0fc3-4ec9-ae67
                     -19b5fdbfbb68"
                     /Sequence="CCCGTATTGACGCCGGG"
                     /created_by="primer3"
                     /label="Amp-F"
     rep_origin      1746..2334
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     2235..2254
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     2488..2505
                     /note="L4440 vector, forward primer"
                     /label="L4440"
     promoter        2658..2688
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2696..2712
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     2701..2723
                     /note="In lacZ gene"
                     /label="M13/pUC Reverse"
     primer_bind     2720..2736
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 rev"
     primer_bind     2720..2736
                     /note="In lacZ gene. Also called M13-rev"
                     /label="M13 Reverse"
     primer_bind     2755..2775
                     /note="T3 promoter, forward primer"
                     /label="T3"
     promoter        2757..2775
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     misc_feature    2788..2793
                     /Recognition_pattern="GAGCT^C"
                     /annotation_group="GAGCTC"
                     /label="SacI"
                     /note="Geneious type: restriction site"
     promoter        2793..3451
                     /created_by="Carson J Bryant"
                     /label="GPD promoter (SacI - XbaI)"
     primer_bind     3336..3362
                     /Sequence="GACGGTAGGTATTGATTGTAATTCTGT"
                     /Hairpin_Tm="None"
                     /Tm="59.3"
                     /Self_Dimer_Tm="None"
                     /%GC="37.0"
                     /modified_by="Carson J Bryant"
                     /label="p414_seqF"
     primer_bind     3336..3362
                     /modified_by="Carson J Bryant"
                     /Mismatches=0
                     /%GC="37.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.3"
                     /annotation_group="158226704947484eb56dc69-bf78-4c2b-9e2b-
                     06223d131925"
                     /Sequence="GACGGTAGGTATTGATTGTAATTCTGT"
                     /created_by="primer3"
                     /label="p414_seqF"
     primer_bind     3338..3361
                     /note="S. cerevisiae GPD promoter, forward primer"
                     /label="GPDpro-F"
     misc_feature    3451..3456
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     primer_bind     3451..3467
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="SK primer"
     primer_bind     3451..3467
                     /note="For pBluescript vector"
                     /label="pBluescriptSK"
     Codon           3481..3483
                     /created_by="Carson J Bryant"
                     /label="Start Codon"
     CDS             3484..3549
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     protein_bind    3550..>3557
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /label="attR1"
     attB1           3551..3576
                     /annotation_group="attB"
                     /label="attB1"
     Junction        3574^3575
                     /Reads_using=2
                     /Reads_supporting_discovery=2
                     /Junction_Type="Insertion"
                     /Insertion_Size=3
                     /Insertion="ACA"
                     /ID="junction 1"
                     /label="ACA"
     gene            join(<3577..>4126,<4127..>5541)
                     /gene="NOP4"
                     /locus_tag="YPL043W"
                     /gene_synonym="NOP77"
                     /db_xref="GeneID:856063"
                     /label="NOP4 gene"
     mRNA            join(<3577..>4126,<4127..>5541)
                     /gene="NOP4"
                     /locus_tag="YPL043W"
                     /gene_synonym="NOP77"
                     /product="mRNA-binding ribosome biosynthesis protein NOP4"
                     /transcript_id="NM_001183857.1"
                     /db_xref="GeneID:856063"
                     /label="NOP4 mRNA"
     source          join(3577..>4126,<4127..5541)
                     /organism="Saccharomyces cerevisiae S288C"
                     /mol_type="genomic DNA"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="XVI"
                     /label="source Saccharomyces cerevisiae S288C"
     CDS             join(3577..>4126,<4127..5541)
                     /gene="NOP4"
                     /locus_tag="YPL043W"
                     /gene_synonym="NOP77"
                     /note="Nucleolar protein; essential for processing and
                     maturation of 27S pre-rRNA and large ribosomal subunit
                     biogenesis; constituent of 66S pre-ribosomal particles;
                     contains four RNA recognition motifs (RRMs)"
                     /codon_start=1
                     /product="mRNA-binding ribosome biosynthesis protein NOP4"
                     /protein_id="NP_015282.1"
                     /db_xref="GI:6325213"
                     /db_xref="GeneID:856063"
                     /db_xref="SGD:S000005964"
                     /translation="MEETIENVEVPSSNVSKQNDDGLDMKTLFVRSIPQDVTDEQLAD
                     FFSNFAPIKHAVVVKDTNKRSRGFGFVSFAVEDDTKEALAKARKTKFNGHILRVDIAK
                     RRDRSKKTSEVVEKSTPESSEKITGQNNEDEDDADGEDSMLKGKPKLIIRNMPWSCRD
                     PVKLKKIFGRYGTVVEATIPRKRDGKLCGFAFVTMKKISNCRIALENTKDLKIDGRKV
                     AVDFAVQKNRWEDYKKAQPEMNDKDDNESGNEDAEENHDDEEDENEEEDRQVDQASKN
                     KESKRKAQNKREDFSVFVRNVPYDATEESLAPHFSKFGSVKYALPVIDKSTGLAKGTA
                     FVAFKDQYTYNECIKNAPAAGSTSLLIGDDVMPEYVYEGRVLSITPTLVREDAGRMAE
                     KNAAKRKEALGKAPGEKDRRNLYLLNEGRVVEGSKMADLLTNTDMEIREKSYKLRVEQ
                     LKKNPSLHLSMTRLAIRNLPRAMNDKALKALARKAVVEFATEVKNKERHPLSKEEIIR
                     STKEKYKFMGPDEIEAQKKKDKKSGVVKQAKVIMEVKGSTAGRSRGYGFVEFRDHKNA
                     LMGLRWLNCHAVTSDEILEGLNDDEKKQVDNDLGKGRRLCVEFAIENSNVVKRRREQL
                     KQARTKRTRPDNEDTGDVGESENKKPKKEEATTPTNPDDKKMGDDIKRIIGFKRKRKH
                     AKK"
                     /label="NOP4 CDS"
     primer_bind     3577..3606
                     /Sequence="ATGGAAGAAACAATAGAAAATGTTGAAGTG"
                     /Hairpin_Tm="39.6"
                     /Tm="59.4"
                     /Self_Dimer_Tm="None"
                     /%GC="30.0"
                     /created_by="primer3"
                     /Extension="GGGGACAAGTTTGTACAAAAAAGCAGGCTTA"
                     /label="Nop4_F"
     domain          3655..3888
                     /created_by="Carson J Bryant"
                     /Transferred_From="NOP4_g"
                     /Transferred_Similarity="100.00%"
                     /label="RRM1"
     domain          join(4015..>4126,<4127..4170)
                     /created_by="Carson J Bryant"
                     /label="RRM2"
     primer_bind     4100..4123
                     /Mismatches=0
                     /Mismatches=0
                     /%GC="54.2"
                     /Hairpin_Tm="38.0"
                     /Self_Dimer_Tm="7.7"
                     /Tm="64.5"
                     /annotation_group="158226417492279c405a33d-109e-4e72-8cea-
                     57e02a442901"
                     /Sequence="TGGAGGCTACCATTCCAAGGAAGC"
                     /created_by="primer3"
                     /label="Nop4_seqF1"
     primer_bind     complement(4106..4126)
                     /Mismatches=0
                     /%GC="52.4"
                     /Hairpin_Tm="32.7"
                     /Self_Dimer_Tm="2.2"
                     /Tm="59.0"
                     /annotation_group="158226516005477ffd525de-c744-4655-9196-
                     7672c243800c"
                     /Sequence="CACGCTTCCTTGGAATGGTAG"
                     /created_by="primer3"
                     /label="Nop4_dEx5_R"
     primer_bind     complement(4106..4126)
                     /Sequence="CACGCTTCCTTGGAATGGTAG"
                     /Hairpin_Tm="32.7"
                     /Tm="59.6"
                     /Self_Dimer_Tm="2.2"
                     /%GC="52.4"
                     /created_by="primer3"
                     /Extension="ATCAACAGCCACCTTTCTAC"
                     /modified_by="Carson J Bryant"
                     /label="NopdEx5R+20"
     misc_feature    4127..4126
                     /Source="referenceToDocument: urn:local:.:qt-aouszze"
                     /label="p414GPD-3xFLAG-Nop4 WT PCR SKIP E5 (concatenated
                     sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     primer_bind     4127..4150
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.8"
                     /annotation_group="158227110260127edbca266-4395-4e09-b23f-
                     1afd9561f7ce"
                     /Sequence="GTAGAAAGGTGGCTGTTGATTTTG"
                     /created_by="primer3"
                     /label="Nop4_dEx5_F"
     primer_bind     4127..4144
                     /Sequence="GTAGAAAGGTGGCTGTTG"
                     /Hairpin_Tm="None"
                     /Tm="53.4"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /Extension="CTACCATTCCAAGGAAGCGTG"
                     /modified_by="Carson J Bryant"
                     /label="NopdE5_GbF2"
     primer_bind     complement(4249..4275)
                     /modified_by="Carson J Bryant"
                     /Mismatches=0
                     /Extension="CTA"
                     /%GC="37.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.6"
                     /annotation_group="158226350966626d07b1bd7-84c3-4d2b-8102-
                     3c87fd886681"
                     /Sequence="TTCATTTTCATCTTCTTCGTCGTCATG"
                     /created_by="primer3"
                     /label="Nop4_dEx8_R"
     exon            4276..4305
                     /created_by="Carson J Bryant"
                     /label="Human Exon 8 alignment frag"
     domain          4348..4644
                     /created_by="Carson J Bryant"
                     /Transferred_From="NOP4_g"
                     /Transferred_Similarity="100.00%"
                     /label="RRM3"
     primer_bind     4481..4504
                     /Mismatches=0
                     /Mismatches=0
                     /%GC="54.2"
                     /Hairpin_Tm="48.3"
                     /Self_Dimer_Tm="21.2"
                     /Tm="64.2"
                     /annotation_group="158226817012449af83b51c-ff4c-4db5-b241-
                     35247fe98ef1"
                     /Sequence="CGGCCTTTGTAGCCTTCAAAGACC"
                     /created_by="primer3"
                     /label="Nop4_seqF2"
     domain          4855..5343
                     /created_by="Carson J Bryant"
                     /Transferred_From="NOP4_g"
                     /Transferred_Similarity="100.00%"
                     /label="RRM4"
     primer_bind     4976..4998
                     /Mismatches=0
                     /Mismatches=0
                     /%GC="39.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="54.8"
                     /annotation_group="15822700838872144e7bf48-d99a-49b6-8493-
                     71b298798abc"
                     /Sequence="GACATCCATTGAGTAAAGAAGAG"
                     /created_by="primer3"
                     /label="Nop4_seqF3"
     primer_bind     complement(5515..5541)
                     /Sequence="CTATTTTTTTGCGTGCTTTCTCTTACG"
                     /Hairpin_Tm="39.4"
                     /Tm="60.0"
                     /Self_Dimer_Tm="None"
                     /%GC="37.0"
                     /created_by="primer3"
                     /Extension="GGGGACCACTTTGTACAAGAAAGCTGGGTA"
                     /label="Nop4_R"
     Junction        join(5539,5543)
                     /Reads_using=1
                     /Reads_supporting_discovery=1
                     /Junction_Type="Deletion"
                     /Deletion_Size=3
                     /Junction_Source="5632+"
                     /Junction_Destination="5636+"
                     /ID="junction 1"
                     /label="junction 1"
     Junction        join(5539,5543)
                     /Reads_using=2
                     /Reads_supporting_discovery=2
                     /Junction_Type="Deletion"
                     /Deletion_Size=3
                     /Junction_Source="5539+"
                     /Junction_Destination="5543+"
                     /ID="junction 2"
                     /label="junction 2"
     misc_feature    5541^5542
                     /Original_Bases="GGGGGATAAGCAATGCTTTCTTATAATGCCAACTTTGTACA
                     AGAAAGCTGGGTCTCGAGCTA"
                     /label="GGGGGATAAGCAATGCTTTCTTATAATGCCAACTTTGTACAAGAAAGCTG
                     GGTCTCGAGCTA"
                     /note="Geneious type: Editing History Deletion"
     attB2           complement(5542..5566)
                     /annotation_group="attB"
                     /label="attB2"
     protein_bind    complement(<5553..5567)
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /label="attR2"
     primer_bind     5568..5595
                     /Mismatches=0
                     /%GC="32.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="158226910365245bdf56f5a-3e11-4026-8749-
                     6555a9a9644a"
                     /Sequence="AGTCATGTAATTAGTTATGTCACGCTTA"
                     /created_by="primer3"
                     /label="Nop4_dEx8_F"
     terminator      5570..5817
                     /gene="S. cerevisiae CYC1"
                     /note="transcription terminator for CYC1"
                     /label="CYC1 terminator"
     primer_bind     complement(5586..5605)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /Pair_Dimer_Tm="None"
                     /Sequence="GGCGTGAATGTAAGCGTGAC"
                     /created_by="primer3"
                     /Product_Size=1821
                     /annotation_group="58239892929201442be2874-be56-42ff-8b4c-
                     44b21d373756urn:sequence:genbank:."
                     /label="5,292 R"
     primer_bind     complement(5586..5604)
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
                     /label="CYC1"
     primer_bind     complement(5612..5631)
                     /Sequence="TTCGGTTAGAGCGGATGTGG"
                     /Hairpin_Tm="39.9"
                     /Tm="59.8"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=1847
                     /modified_by="Carson J Bryant"
                     /label="p414_seqR"
     primer_bind     complement(5612..5631)
                     /modified_by="Carson J Bryant"
                     /Mismatches=0
                     /%GC="55.0"
                     /Hairpin_Tm="39.9"
                     /Self_Dimer_Tm="None"
                     /Tm="59.8"
                     /annotation_group="1582266087873710b22aa21-d9cc-481a-b0f4-
                     13a026329f04"
                     /Sequence="TTCGGTTAGAGCGGATGTGG"
                     /created_by="primer3"
                     /label="p414_seqR"
     primer_bind     complement(5835..5854)
                     /note="T7 promoter, forward primer"
                     /label="T7"
     promoter        complement(5836..5854)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(5864..5881)
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
                     /label="M13 Forward"
     primer_bind     complement(5864..5880)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 fwd"
     primer_bind     complement(5873..5895)
                     /note="In lacZ gene"
                     /label="M13/pUC Forward"
     rep_origin      6025..6480
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     complement(6112..6131)
                     /note="F1 origin, reverse primer"
                     /label="F1ori-R"
     primer_bind     6322..6343
                     /note="F1 origin, forward primer"
                     /label="F1ori-F"
     CDS             complement(6579..7253)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK"
                     /label="TRP1"
     misc_feature    7165..7170
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     promoter        complement(7254..7534)
                     /gene="S. cerevisiae TRP1"
                     /label="TRP1 promoter"
     primer_bind     complement(7551..7570)
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /label="pRS-marker"
     primer_bind     7670..7692
                     /note="pGEX vectors, reverse primer"
                     /label="pGEX 3'"
ORIGIN      
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2761 aaccctcact aaagggaaca aaagctggag ctctcgagtt tatcattatc aatactgcca
     2821 tttcaaagaa tacgtaaata attaatagta gtgattttcc taactttatt tagtcaaaaa
     2881 attagccttt taattctgct gtaacccgta catgccaaaa tagggggcgg gttacacaga
     2941 atatataaca ctgatggtgc ttgggtgaac aggtttattc ctggcatcca ctaaatataa
     3001 tggagcccgc tttttaagct ggcatccaga aaaaaaaaga atcccagcac caaaatattg
     3061 ttttcttcac caaccatcag ttcataggtc cattctctta gcgcaactac agagaacagg
     3121 gcacaaacag gcaaaaaacg ggcacaacct caatggagtg atgcaacctg cctggagtaa
     3181 atgatgacac aaggcaattg acccacgcat gtatctatct cattttctta caccttctat
     3241 taccttctgc tctctctgat ttggaaaaag ctgaaaaaaa aggtttaaac cagttccctg
     3301 aaattattcc cctacttgac taataagtat ataaagacgg taggtattga ttgtaattct
     3361 gtaaatctat ttcttaaact tcttaaattc tacttttata gttagtcttt tttttagttt
     3421 taaaacacca agaacttagt ttcgacggat tctagaacta gtggatcccc cgggctgcag
     3481 atggactaca aagaccatga cggtgattat aaagatcatg atatcgatta caaggatgac
     3541 gatgacaaga caagtttgta caaaaaagca ggcttaatgg aagaaacaat agaaaatgtt
     3601 gaagtgcctt ctagtaatgt ctcaaaacaa aatgacgacg gcctagatat gaagactttg
     3661 tttgtgaggt ctatacccca ggatgtcaca gatgagcagc tggcagattt cttttccaat
     3721 tttgctccga tcaagcatgc ggtagttgtt aaggacacga ataaaaggtc caggggcttc
     3781 gggttcgtta gttttgctgt agaggatgac acaaaagaag ccctagctaa ggccagaaag
     3841 accaaattta acggacatat cttgagggta gacattgcta aaagaagaga tcgttcaaag
     3901 aaaacatcag aagtggttga aaagagcaca cctgagtcat ctgagaaaat aacgggccag
     3961 aacaatgaag atgaggacga tgctgatggt gaagactcca tgctaaaagg taaaccgaaa
     4021 ttaattatca gaaatatgcc atggagttgt cgtgatccag ttaaattgaa aaagatcttt
     4081 ggtagatatg gtaccgtcgt ggaggctacc attccaagga agcgtggtag aaaggtggct
     4141 gttgattttg ctgttcaaaa aaacagatgg gaagattaca aaaaagccca gccagaaatg
     4201 aatgacaaag atgataacga gtcaggaaat gaagatgctg aagaaaacca tgacgacgaa
     4261 gaagatgaaa atgaagagga agatagacaa gtagatcaag cttcaaaaaa caaagaatcg
     4321 aaacgtaaag cacaaaacaa aagggaagat ttttctgttt ttgttcgtaa cgtcccttat
     4381 gatgccactg aagaatcttt ggctccgcac ttcagtaaat ttggcagcgt taaatacgca
     4441 ttacctgtta tcgataagtc tacggggttg gctaaaggta cggcctttgt agccttcaaa
     4501 gaccaatata catataacga gtgtatcaaa aatgcgcctg cggctggctc tacctcttta
     4561 ttgataggtg atgatgtcat gcctgagtat gtgtatgaag gtcgtgttct gtccattact
     4621 ccaacgttag taagagagga tgctggaaga atggcagaaa agaacgccgc caaaagaaag
     4681 gaggccctag gtaaagcgcc aggcgaaaaa gatagacgta acttatattt gctgaatgaa
     4741 ggtagagttg ttgaaggctc aaaaatggct gatttgttaa ccaacactga catggagatc
     4801 agagaaaaat cttacaaatt aagagtggag caactgaaaa agaacccatc tttgcatttg
     4861 tcgatgacta ggttggctat cagaaatctt cctagagcaa tgaatgataa agcattaaag
     4921 gcgttagctc gtaaagctgt cgtggaattt gctacagaag ttaaaaataa ggaaagacat
     4981 ccattgagta aagaagagat tatcagatct acaaaggaaa aatacaagtt catgggtcca
     5041 gatgagattg aagctcaaaa aaagaaagac aaaaaaagtg gtgttgtcaa gcaagcaaaa
     5101 gttataatgg aagttaaagg atcaactgcg ggaagaagta gaggttacgg ttttgtagaa
     5161 ttcagagatc ataaaaacgc tttgatgggc ttaaggtggt tgaattgtca tgccgttacc
     5221 tccgatgaaa ttttagaggg tttgaatgat gatgaaaaga aacaagtaga caatgactta
     5281 ggcaaaggaa gacgtctttg tgttgaattt gcgattgaga actctaacgt cgtaaagaga
     5341 agaagggaac aattgaagca agcaagaacc aagagaacaa gaccagataa tgaagatact
     5401 ggagatgtcg gagaatccga gaataagaag ccaaaaaaag aagaagcaac gacgcccacc
     5461 aatcccgatg acaaaaagat gggcgatgat ataaagagaa taatcgggtt caagcgtaag
     5521 agaaagcacg caaaaaaata gtacccagct ttcttgtaca aagtggtagt catgtaatta
     5581 gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga
     5641 gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga
     5701 acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac
     5761 attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttgcggc
     5821 cggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     5881 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     5941 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     6001 gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6061 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6121 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6181 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6241 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6301 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6361 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6421 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     6481 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca taggcaagtg
     6541 cacaaacaat acttaaataa atactactca gtaataacct atttcttagc atttttgacg
     6601 aaatttgcta ttttgttaga gtcttttaca ccatttgtct ccacacctcc gcttacatca
     6661 acaccaataa cgccatttaa tctaagcgca tcaccaacat tttctggcgt cagtccacca
     6721 gctaacataa aatgtaagct ttcggggctc tcttgccttc caacccagtc agaaatcgag
     6781 ttccaatcca aaagttcacc tgtcccacct gcttctgaat caaacaaggg aataaacgaa
     6841 tgaggtttct gtgaagctgc actgagtagt atgttgcagt cttttggaaa tacgagtctt
     6901 ttaataactg gcaaaccgag gaactcttgg tattcttgcc acgactcatc tccatgcagt
     6961 tggacgatat caatgccgta atcattgacc agagccaaaa catcctcctt aggttgatta
     7021 cgaaacacgc caaccaagta tttcggagtg cctgaactat ttttatatgc ttttacaaga
     7081 cttgaaattt tccttgcaat aaccgggtca attgttctct ttctattggg cacacatata
     7141 atacccagca agtcagcatc ggaatctaga gcacattctg cggcctctgt gctctgcaag
     7201 ccgcaaactt tcaccaatgg accagaacta cctgtgaaat taataacaga catactccaa
     7261 gctgcctttg tgtgcttaat cacgtatact cacgtgctca atagtcacca atgccctccc
     7321 tcttggccct ctccttttct tttttcgacc gaattaattc ttaatcggca aaaaaagaaa
     7381 agctccggat caagattgta cgtaaggtga caagctattt ttcaataaag aatatcttcc
     7441 actactgcca tctggcgtca taactgcaaa gtacacatat attacgatgc tgtctattaa
     7501 atgcttccta tattatatat atagtaatgt cgtttatggt gcactctcag tacaatctgc
     7561 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     7621 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     7681 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
//
