LOCUS       p414GPD-3xFLAG-N        6524 bp    DNA     circular UNA 27-AUG-2019
DEFINITION  an expression clone created from p414GPD-3xFLAG-GW and pDONR221 -
            NOP4_g with attB sites Entry clone (Circularized).
ACCESSION   urn.local...rf-aout1nf
VERSION     urn.local...rf-aout1nf
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          join(1..>3557,<4372..6524)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     complement(10..28)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /label="pBRforEco"
     misc_feature    70..573
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
                     /label="CEN/ARS"
     promoter        610..714
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             715..1575
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     primer_bind     933..970
                     /Mismatches=0
                     /Sequence="GCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGC"
                     /Mismatches=0
                     /annotation_group="0eb63c48-583c-4ef9-9810-9c8fc6e6df75"
                     /label="NopdE5_GbF1"
     primer_bind     complement(933..952)
                     /note="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     primer_bind     complement(934..952)
                     /Mismatches=0
                     /Mismatches=0
                     /%GC="47.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="54.9"
                     /annotation_group="15400746281754105bf13d7a-c872-4000-9658
                     -29e9f4c51561"
                     /Sequence="ATAATACCGCGCCACATAG"
                     /created_by="primer3"
                     /label="NopdE5_GbR2"
     rep_origin      1746..2334
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     2235..2254
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     2488..2505
                     /note="L4440 vector, forward primer"
                     /label="L4440"
     promoter        2658..2688
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2696..2712
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     2701..2723
                     /note="In lacZ gene"
                     /label="M13/pUC Reverse"
     primer_bind     2720..2736
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 rev"
     primer_bind     2720..2736
                     /note="In lacZ gene. Also called M13-rev"
                     /label="M13 Reverse"
     primer_bind     2755..2775
                     /note="T3 promoter, forward primer"
                     /label="T3"
     promoter        2757..2775
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     misc_feature    2788..2793
                     /Recognition_pattern="GAGCT^C"
                     /annotation_group="GAGCTC"
                     /label="SacI"
                     /note="Geneious type: restriction site"
     promoter        2793..3451
                     /created_by="Carson J Bryant"
                     /label="GPD promoter (SacI - XbaI)"
     primer_bind     3336..3362
                     /Sequence="GACGGTAGGTATTGATTGTAATTCTGT"
                     /Hairpin_Tm="None"
                     /Tm="59.3"
                     /Self_Dimer_Tm="None"
                     /%GC="37.0"
                     /modified_by="Carson J Bryant"
                     /label="p414_seqF"
     primer_bind     3336..3362
                     /modified_by="Carson J Bryant"
                     /Mismatches=0
                     /%GC="37.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.3"
                     /annotation_group="158226704947484eb56dc69-bf78-4c2b-9e2b-
                     06223d131925"
                     /Sequence="GACGGTAGGTATTGATTGTAATTCTGT"
                     /created_by="primer3"
                     /label="p414_seqF"
     primer_bind     3338..3361
                     /note="S. cerevisiae GPD promoter, forward primer"
                     /label="GPDpro-F"
     misc_feature    3451..3456
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     primer_bind     3451..3467
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="SK primer"
     primer_bind     3451..3467
                     /note="For pBluescript vector"
                     /label="pBluescriptSK"
     Codon           3481..3483
                     /created_by="Carson J Bryant"
                     /label="Start Codon"
     CDS             3484..3549
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     protein_bind    3550..>3557
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /label="attR1"
     attB1           3551..3576
                     /annotation_group="attB"
                     /label="attB1"
     gene            <3577..>4368
                     /gene="NOP4"
                     /locus_tag="YPL043W"
                     /gene_synonym="NOP77"
                     /db_xref="GeneID:856063"
                     /label="NOP4 gene"
     mRNA            <3577..>4368
                     /gene="NOP4"
                     /locus_tag="YPL043W"
                     /gene_synonym="NOP77"
                     /product="mRNA-binding ribosome biosynthesis protein NOP4"
                     /transcript_id="NM_001183857.1"
                     /db_xref="GeneID:856063"
                     /label="NOP4 mRNA"
     source          3577..>4368
                     /organism="Saccharomyces cerevisiae S288C"
                     /mol_type="genomic DNA"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="XVI"
                     /label="source Saccharomyces cerevisiae S288C"
     CDS             3577..>4371
                     /gene="NOP4"
                     /locus_tag="YPL043W"
                     /gene_synonym="NOP77"
                     /note="Nucleolar protein; essential for processing and
                     maturation of 27S pre-rRNA and large ribosomal subunit
                     biogenesis; constituent of 66S pre-ribosomal particles;
                     contains four RNA recognition motifs (RRMs)"
                     /codon_start=1
                     /product="mRNA-binding ribosome biosynthesis protein NOP4"
                     /protein_id="NP_015282.1"
                     /db_xref="GI:6325213"
                     /db_xref="GeneID:856063"
                     /db_xref="SGD:S000005964"
                     /translation="MEETIENVEVPSSNVSKQNDDGLDMKTLFVRSIPQDVTDEQLAD
                     FFSNFAPIKHAVVVKDTNKRSRGFGFVSFAVEDDTKEALAKARKTKFNGHILRVDIAK
                     RRDRSKKTSEVVEKSTPESSEKITGQNNEDEDDADGEDSMLKGKPKLIIRNMPWSCRD
                     PVKLKKIFGRYGTVVEATIPRKRDGKLCGFAFVTMKKISNCRIALENTKDLKIDGRKV
                     AVDFAVQKNRWEDYKKAQPEMNDKDDNESGNEDAEENHDDEEDENEEEDRQVDQASKN
                     KESKRKAQNKREDFSVFVRNVPYDATEESLAPHFSKFGSVKYALPVIDKSTGLAKGTA
                     FVAFKDQYTYNECIKNAPAAGSTSLLIGDDVMPEYVYEGRVLSITPTLVREDAGRMAE
                     KNAAKRKEALGKAPGEKDRRNLYLLNEGRVVEGSKMADLLTNTDMEIREKSYKLRVEQ
                     LKKNPSLHLSMTRLAIRNLPRAMNDKALKALARKAVVEFATEVKNKERHPLSKEEIIR
                     STKEKYKFMGPDEIEAQKKKDKKSGVVKQAKVIMEVKGSTAGRSRGYGFVEFRDHKNA
                     LMGLRWLNCHAVTSDEILEGLNDDEKKQVDNDLGKGRRLCVEFAIENSNVVKRRREQL
                     KQARTKRTRPDNEDTGDVGESENKKPKKEEATTPTNPDDKKMGDDIKRIIGFKRKRKH
                     AKK"
                     /label="NOP4 CDS"
     primer_bind     3577..3606
                     /Sequence="ATGGAAGAAACAATAGAAAATGTTGAAGTG"
                     /Hairpin_Tm="39.6"
                     /Tm="59.4"
                     /Self_Dimer_Tm="None"
                     /%GC="30.0"
                     /created_by="primer3"
                     /Extension="GGGGACAAGTTTGTACAAAAAAGCAGGCTTA"
                     /label="Nop4_F"
     domain          3655..3888
                     /created_by="Carson J Bryant"
                     /Transferred_From="NOP4_g"
                     /Transferred_Similarity="100.00%"
                     /label="RRM1"
     domain          4015..4263
                     /created_by="Carson J Bryant"
                     /label="RRM2"
     primer_bind     4100..4123
                     /Mismatches=0
                     /Mismatches=0
                     /%GC="54.2"
                     /Hairpin_Tm="38.0"
                     /Self_Dimer_Tm="7.7"
                     /Tm="64.5"
                     /annotation_group="158226417492279c405a33d-109e-4e72-8cea-
                     57e02a442901"
                     /Sequence="TGGAGGCTACCATTCCAAGGAAGC"
                     /created_by="primer3"
                     /label="Nop4_seqF1"
     primer_bind     complement(4105..4126)
                     /Mismatches=0
                     /Mismatches=0
                     /%GC="54.5"
                     /Hairpin_Tm="46.5"
                     /Self_Dimer_Tm="2.2"
                     /Tm="61.8"
                     /annotation_group="1540074571006597a6690231-47c4-40c3-bd22
                     -de335e73ce8e"
                     /Sequence="CACGCTTCCTTGGAATGGTAGC"
                     /created_by="primer3"
                     /label="NopdE5_GbR1"
     primer_bind     complement(4106..4126)
                     /Mismatches=0
                     /%GC="52.4"
                     /Hairpin_Tm="32.7"
                     /Self_Dimer_Tm="2.2"
                     /Tm="59.0"
                     /annotation_group="158226516005477ffd525de-c744-4655-9196-
                     7672c243800c"
                     /Sequence="CACGCTTCCTTGGAATGGTAG"
                     /created_by="primer3"
                     /label="Nop4_dEx5_R"
     exon            4127..4219
                     /created_by="Carson J Bryant"
                     /label="Human Exon 5"
     primer_bind     4220..4243
                     /Mismatches=0
                     /%GC="41.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.8"
                     /annotation_group="158227110260127edbca266-4395-4e09-b23f-
                     1afd9561f7ce"
                     /Sequence="GTAGAAAGGTGGCTGTTGATTTTG"
                     /created_by="primer3"
                     /label="Nop4_dEx5_F"
     primer_bind     complement(4342..4368)
                     /modified_by="Carson J Bryant"
                     /Mismatches=0
                     /Extension="CTA"
                     /%GC="37.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.6"
                     /annotation_group="158226350966626d07b1bd7-84c3-4d2b-8102-
                     3c87fd886681"
                     /Sequence="TTCATTTTCATCTTCTTCGTCGTCATG"
                     /created_by="primer3"
                     /label="Nop4_dEx8_R"
     primer_bind     complement(4342..4368)
                     /Sequence="TTCATTTTCATCTTCTTCGTCGTCATG"
                     /Hairpin_Tm="None"
                     /Tm="60.3"
                     /Self_Dimer_Tm="None"
                     /%GC="37.0"
                     /Extension="GACATAACTAATTACATGACTCTA"
                     /modified_by="Carson J Bryant"
                     /label="Nop4dEx8R+20"
     Stop_codon      complement(4369..4371)
                     /Color="-1"
                     /label="stop codon"
     misc_feature    4372..4371
                     /Source="referenceToDocument: urn:local:.:p9-aousn6z"
                     /label="p414GPD-3xFLAG-Nop4 WT PCR STOP before E8
                     (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     primer_bind     4372..4399
                     /Mismatches=0
                     /%GC="32.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="158226910365245bdf56f5a-3e11-4026-8749-
                     6555a9a9644a"
                     /Sequence="AGTCATGTAATTAGTTATGTCACGCTTA"
                     /created_by="primer3"
                     /label="Nop4_dEx8_F"
     primer_bind     4372..4394
                     /Sequence="AGTCATGTAATTAGTTATGTCAC"
                     /Hairpin_Tm="None"
                     /Tm="51.7"
                     /Self_Dimer_Tm="None"
                     /%GC="30.4"
                     /created_by="primer3"
                     /Extension="AAGAAGATGAAAATGAATAG"
                     /modified_by="Carson J Bryant"
                     /label="NopdE8_GbF2"
     terminator      4374..4621
                     /gene="S. cerevisiae CYC1"
                     /note="transcription terminator for CYC1"
                     /label="CYC1 terminator"
     primer_bind     complement(4390..4409)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /Pair_Dimer_Tm="None"
                     /Sequence="GGCGTGAATGTAAGCGTGAC"
                     /created_by="primer3"
                     /Product_Size=1821
                     /annotation_group="58239892929201442be2874-be56-42ff-8b4c-
                     44b21d373756urn:sequence:genbank:."
                     /label="5,292 R"
     primer_bind     complement(4390..4408)
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
                     /label="CYC1"
     primer_bind     complement(4416..4435)
                     /Sequence="TTCGGTTAGAGCGGATGTGG"
                     /Hairpin_Tm="39.9"
                     /Tm="59.8"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=1847
                     /modified_by="Carson J Bryant"
                     /label="p414_seqR"
     primer_bind     complement(4416..4435)
                     /modified_by="Carson J Bryant"
                     /Mismatches=0
                     /%GC="55.0"
                     /Hairpin_Tm="39.9"
                     /Self_Dimer_Tm="None"
                     /Tm="59.8"
                     /annotation_group="1582266087873710b22aa21-d9cc-481a-b0f4-
                     13a026329f04"
                     /Sequence="TTCGGTTAGAGCGGATGTGG"
                     /created_by="primer3"
                     /label="p414_seqR"
     primer_bind     complement(4639..4658)
                     /note="T7 promoter, forward primer"
                     /label="T7"
     promoter        complement(4640..4658)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(4668..4685)
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
                     /label="M13 Forward"
     primer_bind     complement(4668..4684)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 fwd"
     primer_bind     complement(4677..4699)
                     /note="In lacZ gene"
                     /label="M13/pUC Forward"
     Junction        join(4823,4828)
                     /Reads_using=1
                     /Reads_supporting_discovery=1
                     /Junction_Type="Deletion"
                     /Deletion_Size=4
                     /Junction_Source="4823+"
                     /Junction_Destination="4828+"
                     /ID="junction 1"
                     /label="junction 1"
     rep_origin      4829..5284
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     complement(4916..4935)
                     /note="F1 origin, reverse primer"
                     /label="F1ori-R"
     primer_bind     5126..5147
                     /note="F1 origin, forward primer"
                     /label="F1ori-F"
     CDS             complement(5383..6057)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK"
                     /label="TRP1"
     misc_feature    5969..5974
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     promoter        complement(6058..6338)
                     /gene="S. cerevisiae TRP1"
                     /label="TRP1 promoter"
     primer_bind     complement(6355..6374)
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /label="pRS-marker"
     primer_bind     6474..6496
                     /note="pGEX vectors, reverse primer"
                     /label="pGEX 3'"
ORIGIN      
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2761 aaccctcact aaagggaaca aaagctggag ctctcgagtt tatcattatc aatactgcca
     2821 tttcaaagaa tacgtaaata attaatagta gtgattttcc taactttatt tagtcaaaaa
     2881 attagccttt taattctgct gtaacccgta catgccaaaa tagggggcgg gttacacaga
     2941 atatataaca ctgatggtgc ttgggtgaac aggtttattc ctggcatcca ctaaatataa
     3001 tggagcccgc tttttaagct ggcatccaga aaaaaaaaga atcccagcac caaaatattg
     3061 ttttcttcac caaccatcag ttcataggtc cattctctta gcgcaactac agagaacagg
     3121 gcacaaacag gcaaaaaacg ggcacaacct caatggagtg atgcaacctg cctggagtaa
     3181 atgatgacac aaggcaattg acccacgcat gtatctatct cattttctta caccttctat
     3241 taccttctgc tctctctgat ttggaaaaag ctgaaaaaaa aggtttaaac cagttccctg
     3301 aaattattcc cctacttgac taataagtat ataaagacgg taggtattga ttgtaattct
     3361 gtaaatctat ttcttaaact tcttaaattc tacttttata gttagtcttt tttttagttt
     3421 taaaacacca agaacttagt ttcgacggat tctagaacta gtggatcccc cgggctgcag
     3481 atggactaca aagaccatga cggtgattat aaagatcatg atatcgatta caaggatgac
     3541 gatgacaaga caagtttgta caaaaaagca ggcttaatgg aagaaacaat agaaaatgtt
     3601 gaagtgcctt ctagtaatgt ctcaaaacaa aatgacgacg gcctagatat gaagactttg
     3661 tttgtgaggt ctatacccca ggatgtcaca gatgagcagc tggcagattt cttttccaat
     3721 tttgctccga tcaagcatgc ggtagttgtt aaggacacga ataaaaggtc caggggcttc
     3781 gggttcgtta gttttgctgt agaggatgac acaaaagaag ccctagctaa ggccagaaag
     3841 accaaattta acggacatat cttgagggta gacattgcta aaagaagaga tcgttcaaag
     3901 aaaacatcag aagtggttga aaagagcaca cctgagtcat ctgagaaaat aacgggccag
     3961 aacaatgaag atgaggacga tgctgatggt gaagactcca tgctaaaagg taaaccgaaa
     4021 ttaattatca gaaatatgcc atggagttgt cgtgatccag ttaaattgaa aaagatcttt
     4081 ggtagatatg gtaccgtcgt ggaggctacc attccaagga agcgtgatgg taaattgtgt
     4141 ggatttgcgt ttgttactat gaagaaaatc agtaattgta gaattgcttt ggaaaacacc
     4201 aaagacttga aaattgatgg tagaaaggtg gctgttgatt ttgctgttca aaaaaacaga
     4261 tgggaagatt acaaaaaagc ccagccagaa atgaatgaca aagatgataa cgagtcagga
     4321 aatgaagatg ctgaagaaaa ccatgacgac gaagaagatg aaaatgaata gagtcatgta
     4381 attagttatg tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga
     4441 aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt
     4501 aagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg
     4561 taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg
     4621 cggccggtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     4681 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4741 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4801 ttgcgcagcc tgaatggcga atggcgcgac gcgccctgta gcggcgcatt aagcgcggcg
     4861 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4921 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4981 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5041 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5101 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5161 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5221 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     5281 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataggca
     5341 agtgcacaaa caatacttaa ataaatacta ctcagtaata acctatttct tagcattttt
     5401 gacgaaattt gctattttgt tagagtcttt tacaccattt gtctccacac ctccgcttac
     5461 atcaacacca ataacgccat ttaatctaag cgcatcacca acattttctg gcgtcagtcc
     5521 accagctaac ataaaatgta agctttcggg gctctcttgc cttccaaccc agtcagaaat
     5581 cgagttccaa tccaaaagtt cacctgtccc acctgcttct gaatcaaaca agggaataaa
     5641 cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg cagtcttttg gaaatacgag
     5701 tcttttaata actggcaaac cgaggaactc ttggtattct tgccacgact catctccatg
     5761 cagttggacg atatcaatgc cgtaatcatt gaccagagcc aaaacatcct ccttaggttg
     5821 attacgaaac acgccaacca agtatttcgg agtgcctgaa ctatttttat atgcttttac
     5881 aagacttgaa attttccttg caataaccgg gtcaattgtt ctctttctat tgggcacaca
     5941 tataataccc agcaagtcag catcggaatc tagagcacat tctgcggcct ctgtgctctg
     6001 caagccgcaa actttcacca atggaccaga actacctgtg aaattaataa cagacatact
     6061 ccaagctgcc tttgtgtgct taatcacgta tactcacgtg ctcaatagtc accaatgccc
     6121 tccctcttgg ccctctcctt ttcttttttc gaccgaatta attcttaatc ggcaaaaaaa
     6181 gaaaagctcc ggatcaagat tgtacgtaag gtgacaagct atttttcaat aaagaatatc
     6241 ttccactact gccatctggc gtcataactg caaagtacac atatattacg atgctgtcta
     6301 ttaaatgctt cctatattat atatatagta atgtcgttta tggtgcactc tcagtacaat
     6361 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     6421 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     6481 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcga
//
