LOCUS       p414GPD-3xFLAG-R        8035 bp    DNA     circular UNA 11-OCT-2019
DEFINITION  an expression clone created from p414GPD-3xFLAG-GW and pDONR-RBM28.
ACCESSION   urn.local...1ah-ap4a35f
VERSION     urn.local...1ah-ap4a35f
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          join(1..>3557,<5868..8035)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     complement(10..28)
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /label="pBRforEco"
     misc_feature    70..573
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
                     /label="CEN/ARS"
     promoter        610..714
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             715..1575
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     primer_bind     complement(933..952)
                     /note="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     rep_origin      1746..2334
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     2235..2254
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     2488..2505
                     /note="L4440 vector, forward primer"
                     /label="L4440"
     promoter        2658..2688
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2696..2712
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     2703..2722
                     /%GC="40.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="52.5"
                     /Sequence="CGGATAACAATTTCACACAG"
                     /created_by="primer3"
                     /Transferred_From="M13-48REV"
                     /Transferred_Similarity="100.00%"
                     /label="M13-48REV"
     primer_bind     2720..2736
                     /%GC="47.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="49.5"
                     /Sequence="CAGGAAACAGCTATGAC"
                     /created_by="primer3"
                     /Transferred_From="M13R"
                     /Transferred_Similarity="100.00%"
                     /label="M13R"
     primer_bind     2758..2777
                     /%GC="40.0"
                     /Hairpin_Tm="57.2"
                     /Self_Dimer_Tm="15.8"
                     /Tm="53.3"
                     /Sequence="ATTAACCCTCACTAAAGGGA"
                     /created_by="primer3"
                     /Transferred_From="T3"
                     /Transferred_Similarity="100.00%"
                     /label="T3"
     misc_feature    2788..2793
                     /Recognition_pattern="GAGCT^C"
                     /annotation_group="GAGCTC"
                     /label="SacI"
                     /note="Geneious type: restriction site"
     promoter        2793..3451
                     /created_by="Carson J Bryant"
                     /label="GPD promoter (SacI - XbaI)"
     primer_bind     3336..3362
                     /Sequence="GACGGTAGGTATTGATTGTAATTCTGT"
                     /Hairpin_Tm="None"
                     /Tm="59.3"
                     /Self_Dimer_Tm="None"
                     /%GC="37.0"
                     /modified_by="Carson J Bryant"
                     /label="p414_seqF"
     primer_bind     3338..3361
                     /note="S. cerevisiae GPD promoter, forward primer"
                     /label="GPDpro-F"
     misc_feature    3451..3456
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     primer_bind     3451..3467
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="SK primer"
     primer_bind     3451..3467
                     /note="For pBluescript vector"
                     /label="pBluescriptSK"
     Codon           3481..3483
                     /created_by="Carson J Bryant"
                     /label="Start Codon"
     CDS             3484..3549
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     protein_bind    3550..>3557
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /label="attR1"
     attB1           3551..3576
                     /annotation_group="attB"
                     /label="attB1"
     primer_bind     3554..3573
                     /Mismatches=0
                     /%GC="40.0"
                     /Hairpin_Tm="31.3"
                     /Self_Dimer_Tm="22.1"
                     /Tm="54.2"
                     /annotation_group="1129375496403353c70319da-4688-4fa8-a42a
                     -f4409d229897"
                     /Sequence="GTTTGTACAAAAAAGCAGGC"
                     /created_by="primer3"
                     /label="ATTB1"
     Junction        3574^3575
                     /Reads_using=4
                     /Reads_supporting_discovery=4
                     /Junction_Type="Insertion"
                     /Insertion_Size=3
                     /Insertion="ACA"
                     /ID="junction 1"
                     /label="ACA"
     CDS             3577..5856
                     /created_by="Carson J Bryant"
                     /label="CDS"
     exon            3577..3694
                     /note="Exon 1"
                     /ugene_name="Exon"
                     /label="Exon 1"
     primer_bind     3577..3596
                     /Sequence="ATGGCCGGCCTGACCTTATT"
                     /annotation_group="30885168977740444fdd095-8a24-4d26-9e74-
                     dd2d47202a2dNM_018077.2_pre-mRNA"
                     /modified_by="Carson J Bryant"
                     /Hairpin_Tm="36.9"
                     /Tm="62.7"
                     /Self_Dimer_Tm="43.3"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=2280
                     /created_by="primer3"
                     /Extension="GGGGACAAGTTTGTACAAAAAAGCAGGCTTA"
                     /label="R28_CDS_F"
     exon            3695..3853
                     /note="Exon 2"
                     /ugene_name="Exon"
                     /label="Exon 2"
     exon            3854..3948
                     /note="Exon 3"
                     /ugene_name="Exon"
                     /label="Exon 3"
     exon            3949..4024
                     /note="Exon 4"
                     /ugene_name="Exon"
                     /label="Exon 4"
     primer_bind     3949..3973
                     /modified_by="Carson J Bryant"
                     /Mismatches=0
                     /Extension="ACTGGGTACC"
                     /%GC="40.0"
                     /Hairpin_Tm="37.8"
                     /Self_Dimer_Tm="None"
                     /Tm="59.0"
                     /annotation_group="1259747908112596eef1f9c6-94f3-45e4-b670
                     -15ed7b45bb19"
                     /Sequence="TGTTCAGAAGATGACTTGAAGACAG"
                     /created_by="primer3"
                     /label="E5_KpnI_F+4"
     primer_bind     complement(3999..4024)
                     /Sequence="CTGGTTTCCTAGGGATATTTACTTCC"
                     /Hairpin_Tm="None"
                     /Tm="59.2"
                     /Self_Dimer_Tm="9.5"
                     /%GC="42.3"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="R28_dEx5_R"
     exon            4025..4117
                     /note="Exon 5"
                     /ugene_name="Exon"
                     /label="Exon 5"
     primer_bind     4029..4048
                     /Sequence="GAAGATGCGCGGTTTTGGTT"
                     /Hairpin_Tm="None"
                     /Tm="60.0"
                     /Self_Dimer_Tm="8.8"
                     /%GC="50.0"
                     /modified_by="Carson J Bryant"
                     /label="E5_int_F"
     misc_feature    4117
                     /note="RBM28 base 541 -- 541+1_541+2_delinsA in adjoining
                     intron"
                     /ugene_name="Mutation"
                     /label="misc"
     exon            4118..4189
                     /note="Exon 6"
                     /ugene_name="Exon"
                     /label="Exon 6"
     primer_bind     4118..4134
                     /Sequence="GCCGGACAGTGGCTGTG"
                     /Hairpin_Tm="49.1"
                     /Tm="62.2"
                     /Self_Dimer_Tm="5.1"
                     /%GC="70.6"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="R28_dEx5_F"
     exon            4190..4385
                     /note="Exon 7"
                     /ugene_name="Exon"
                     /label="Exon 7"
     primer_bind     complement(4363..4385)
                     /Sequence="CTCTTCTGAATTTGCACAGGCTT"
                     /Hairpin_Tm="41.9"
                     /Tm="59.9"
                     /Self_Dimer_Tm="2.6"
                     /%GC="43.5"
                     /created_by="primer3"
                     /label="R28_dEx8_R"
     exon            4386..4522
                     /note="Exon 8"
                     /ugene_name="Exon"
                     /label="Exon 8"
     primer_bind     complement(4470..4489)
                     /Sequence="TCTGAGCCAGTTCCTCTCCA"
                     /Hairpin_Tm="32.6"
                     /Tm="59.9"
                     /Self_Dimer_Tm="2.7"
                     /%GC="55.0"
                     /modified_by="Carson J Bryant"
                     /label="E8_int_R"
     misc_feature    4522
                     /note="G&gt;T, Ala316Ser"
                     /ugene_name="Mutation"
                     /label="G&gt;T, Ala316Ser"
     exon            4523..4595
                     /note="Exon 9"
                     /ugene_name="Exon"
                     /label="Exon 9"
     primer_bind     4523..4548
                     /Sequence="CTGTGCAAGTCTCAAACAAAAAGAAG"
                     /Hairpin_Tm="None"
                     /Tm="59.7"
                     /Self_Dimer_Tm="None"
                     /%GC="38.5"
                     /created_by="primer3"
                     /label="R28_dEx8_F"
     primer_bind     complement(4569..4595)
                     /modified_by="Carson J Bryant"
                     /Mismatches=0
                     /Extension="ACTGGCGGCCGC"
                     /%GC="33.3"
                     /Hairpin_Tm="38.0"
                     /Self_Dimer_Tm="11.8"
                     /Tm="57.8"
                     /annotation_group="1259518623450359aab367e9-f2ba-41ef-ba22
                     -c86615873e08"
                     /Sequence="CTGATAAAAACAGTTTTCCCTTCATTC"
                     /created_by="primer3"
                     /label="E8_NotI_R+4"
     exon            4596..4705
                     /note="Exon 10"
                     /ugene_name="Exon"
                     /label="Exon 10"
     exon            4706..4779
                     /note="Exon 11"
                     /ugene_name="Exon"
                     /label="Exon 11"
     exon            4780..4915
                     /note="Exon 12"
                     /ugene_name="Exon"
                     /label="Exon 12"
     primer_bind     4813..4832
                     /Sequence="AAGGTTGACTTGGCGGTGAC"
                     /Hairpin_Tm="38.2"
                     /Tm="61.6"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /created_by="primer3"
                     /label="R28_E12_F"
     exon            4916..4980
                     /note="Exon 13"
                     /ugene_name="Exon"
                     /label="Exon 13"
     exon            4981..5139
                     /note="Exon 14"
                     /ugene_name="Exon"
                     /label="Exon 14"
     exon            5140..5289
                     /note="Exon 15"
                     /ugene_name="Exon"
                     /label="Exon 15"
     exon            5290..5364
                     /note="Exon 16"
                     /ugene_name="Exon"
                     /label="Exon 16"
     exon            5365..5621
                     /note="Exon 17"
                     /ugene_name="Exon"
                     /label="Exon 17"
     exon            5622..5721
                     /note="Exon 18"
                     /ugene_name="Exon"
                     /label="Exon 18"
     exon            5722..5856
                     /note="Exon 19"
                     /ugene_name="Exon"
                     /label="Exon 19"
     primer_bind     complement(5832..5856)
                     /Sequence="TCAACTATCAAACCATTTGCTCCTC"
                     /Hairpin_Tm="40.4"
                     /Tm="59.7"
                     /Self_Dimer_Tm="None"
                     /%GC="40.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=2280
                     /created_by="primer3"
                     /annotation_group="30885168977740444fdd095-8a24-4d26-9e74-
                     dd2d47202a2dNM_018077.2_pre-mRNA"
                     /modified_by="Carson J Bryant"
                     /Extension="GGGGACCACTTTGTACAAGAAAGCTGGGTA"
                     /label="R28_CDS_R"
     Junction        join(5854,5858)
                     /Reads_using=1
                     /Reads_supporting_discovery=1
                     /Junction_Type="Deletion"
                     /Deletion_Size=3
                     /Junction_Source="2948+"
                     /Junction_Destination="2952+"
                     /ID="junction 1"
                     /label="junction 1"
     attB2           complement(5857..5881)
                     /annotation_group="attB"
                     /label="attB2"
     primer_bind     complement(5858..5881)
                     /Mismatches=0
                     /%GC="45.8"
                     /Hairpin_Tm="34.3"
                     /Self_Dimer_Tm="8.6"
                     /Tm="61.5"
                     /annotation_group="1129375560678951cd803021-6ba1-44e2-a7b9
                     -3b41011335f3"
                     /Sequence="CCACTTTGTACAAGAAAGCTGGGT"
                     /created_by="primer3"
                     /label="ATTB2"
     protein_bind    complement(<5868..5882)
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /label="attR2"
     primer_bind     5883..5910
                     /Mismatches=0
                     /%GC="32.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="15308923527752404f72f9ee-8ac4-45c8-b110
                     -2d4e49e5db7f"
                     /Sequence="AGTCATGTAATTAGTTATGTCACGCTTA"
                     /created_by="primer3"
                     /label="Nop4_dEx8_F"
     primer_bind     5883..5910
                     /Mismatches=0
                     /%GC="32.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.1"
                     /annotation_group="1530899007092485bb8b8438-10dc-4c63-8f4e
                     -6d30e1242587"
                     /Sequence="AGTCATGTAATTAGTTATGTCACGCTTA"
                     /created_by="primer3"
                     /label="Nop4_dEx8_F"
     terminator      5885..6132
                     /gene="S. cerevisiae CYC1"
                     /note="transcription terminator for CYC1"
                     /label="CYC1 terminator"
     primer_bind     complement(5901..5920)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /Pair_Dimer_Tm="None"
                     /Sequence="GGCGTGAATGTAAGCGTGAC"
                     /created_by="primer3"
                     /Product_Size=1821
                     /annotation_group="58239892929201442be2874-be56-42ff-8b4c-
                     44b21d373756urn:sequence:genbank:."
                     /label="5,292 R"
     primer_bind     complement(5901..5919)
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
                     /label="CYC1"
     primer_bind     complement(5927..5946)
                     /Sequence="TTCGGTTAGAGCGGATGTGG"
                     /Hairpin_Tm="39.9"
                     /Tm="59.8"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=1847
                     /modified_by="Carson J Bryant"
                     /label="p414_seqR"
     primer_bind     complement(6150..6169)
                     /%GC="40.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="4.4"
                     /Tm="50.6"
                     /Sequence="TAATACGACTCACTATAGGG"
                     /created_by="primer3"
                     /Transferred_From="T7"
                     /Transferred_Similarity="100.00%"
                     /label="T7"
     primer_bind     complement(6180..6195)
                     /%GC="56.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="2.2"
                     /Tm="53.0"
                     /Sequence="GTAAAACGACGGCCAG"
                     /created_by="primer3"
                     /Transferred_From="M13F"
                     /Transferred_Similarity="100.00%"
                     /label="M13F"
     primer_bind     complement(6199..6215)
                     /%GC="52.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="53.1"
                     /Sequence="GTTTTCCCAGTCACGAC"
                     /created_by="primer3"
                     /Transferred_From="M13-40FOR"
                     /Transferred_Similarity="100.00%"
                     /label="M13-40FOR"
     rep_origin      6340..6795
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     complement(6427..6446)
                     /note="F1 origin, reverse primer"
                     /label="F1ori-R"
     primer_bind     6637..6658
                     /note="F1 origin, forward primer"
                     /label="F1ori-F"
     CDS             complement(6894..7568)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK"
                     /label="TRP1"
     misc_feature    7480..7485
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     promoter        complement(7569..7849)
                     /gene="S. cerevisiae TRP1"
                     /label="TRP1 promoter"
     primer_bind     complement(7866..7885)
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /label="pRS-marker"
     primer_bind     7985..8007
                     /note="pGEX vectors, reverse primer"
                     /label="pGEX 3'"
     primer_bind     8026..21
                     /Mismatches=0
                     /Mismatches=0
                     /%GC="58.1"
                     /Hairpin_Tm="48.8"
                     /Self_Dimer_Tm="39.4"
                     /Tm="73.2"
                     /annotation_group="15308924740331754912f595-b40d-45e4-b12c
                     -2b781163127a"
                     /Sequence="AAACGCGCGAGACGAAAGGGCCTCGTGATAC"
                     /created_by="primer3"
                     /label="R28dE5_GbF1"
     primer_bind     8026..21
                     /Mismatches=0
                     /Mismatches=0
                     /%GC="58.1"
                     /Hairpin_Tm="48.8"
                     /Self_Dimer_Tm="39.4"
                     /Tm="73.2"
                     /annotation_group="15308990728002966e186df9-af00-4e2e-9a4c
                     -4fd2df3470dd"
                     /Sequence="AAACGCGCGAGACGAAAGGGCCTCGTGATAC"
                     /created_by="primer3"
                     /label="R28dE5_GbF1"
ORIGIN      
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2761 aaccctcact aaagggaaca aaagctggag ctctcgagtt tatcattatc aatactgcca
     2821 tttcaaagaa tacgtaaata attaatagta gtgattttcc taactttatt tagtcaaaaa
     2881 attagccttt taattctgct gtaacccgta catgccaaaa tagggggcgg gttacacaga
     2941 atatataaca ctgatggtgc ttgggtgaac aggtttattc ctggcatcca ctaaatataa
     3001 tggagcccgc tttttaagct ggcatccaga aaaaaaaaga atcccagcac caaaatattg
     3061 ttttcttcac caaccatcag ttcataggtc cattctctta gcgcaactac agagaacagg
     3121 gcacaaacag gcaaaaaacg ggcacaacct caatggagtg atgcaacctg cctggagtaa
     3181 atgatgacac aaggcaattg acccacgcat gtatctatct cattttctta caccttctat
     3241 taccttctgc tctctctgat ttggaaaaag ctgaaaaaaa aggtttaaac cagttccctg
     3301 aaattattcc cctacttgac taataagtat ataaagacgg taggtattga ttgtaattct
     3361 gtaaatctat ttcttaaact tcttaaattc tacttttata gttagtcttt tttttagttt
     3421 taaaacacca agaacttagt ttcgacggat tctagaacta gtggatcccc cgggctgcag
     3481 atggactaca aagaccatga cggtgattat aaagatcatg atatcgatta caaggatgac
     3541 gatgacaaga caagtttgta caaaaaagca ggcttaatgg ccggcctgac cttatttgtg
     3601 ggccgcctcc cgccctcggc ccgcagtgag cagctggagg aactgttcag tcaggtgggg
     3661 ccggtgaagc agtgcttcgt ggtgactgaa aaagggagta aggcatgtcg aggctttggc
     3721 tatgtcactt tttcaatgct ggaagatgtt cagagggccc tcaaggagat taccaccttt
     3781 gaaggttgca agatcaacgt gactgttgcc aagaaaaaac tgaggaacaa gacaaaggaa
     3841 aaggggaaaa atgaaaactc agagtgccca aagaaggagc cgaaggctaa aaaagccaaa
     3901 gtggcagata agaaagccag attaattatt cggaacctga gctttaagtg ttcagaagat
     3961 gacttgaaga cagtatttgc tcaatttgga gctgtcctgg aagtaaatat ccctaggaaa
     4021 ccagatggga agatgcgcgg ttttggtttt gttcagttca aaaacctcct agaagcaggt
     4081 aaagctctca aaggcatgaa catgaaagag ataaaaggcc ggacagtggc tgtggattgg
     4141 gccgtggcaa aggataaata taaagataca cagtctgttt ctgctatagg tgaggaaaag
     4201 agccatgaat ctaaacatca ggaatcagtt aaaaagaagg gcagagagga agaggatatg
     4261 gaagaggaag aaaacgatga tgatgacgat gatgatgatg aagaagatgg ggtttttgat
     4321 gatgaagatg aagaggaaga gaatatagaa tcaaaggtga ccaagcctgt gcaaattcag
     4381 aagagagcag tcaagagacc agcccctgca aaaagcagtg atcattctga ggaggacagt
     4441 gacctagagg aaagcgatag tattgatgat ggagaggaac tggctcagag tgataccagc
     4501 actgaggagc aagaggataa agctgtgcaa gtctcaaaca aaaagaagag gaaattaccc
     4561 tctgatgtga atgaagggaa aactgttttt atcagaaatc tgtcctttga ctcagaagaa
     4621 gaagaacttg gggagcttct ccaacagttt ggagaactca aatatgtccg cattgtcttg
     4681 catccagaca cagagcattc taaaggttgt gcatttgccc agttcatgac tcaagaagca
     4741 gctcagaaat gccttctagc tgcttctcca gagaatgagg ctggtgggct taaactggat
     4801 ggccggcagc tcaaggttga cttggcggtg acccgtgatg aggctgcaaa gcttcagacg
     4861 acgaaggtga agaagccgac tggcacccgg aatctctatc tggcccgaga aggcttgatt
     4921 cgtgctggga cgaaggctgc agagggtgtg agtgctgctg atatggccaa aagagaacgg
     4981 tttgagctgc tgaagcatca gaaactcaag gaccagaata tctttgtctc ccgaaccagg
     5041 ctctgcctgc acaatctccc aaaggctgta gatgacaaac agctcagaaa gctgctgctg
     5101 agtgctacta gtggagagaa aggggtgcgc atcaaggagt gtagagtgat gcgagacctc
     5161 aaaggagttc atgggaacat gaagggtcag tccctgggct acgcctttgc ggagttccaa
     5221 gagcacgagc atgccctgaa agccctccgc ctcatcaaca acaatccaga aatctttggg
     5281 cctctgaaga gaccaatagt ggagttctct ttagaagatc gaagaaaact taaaatgaag
     5341 gaattaagga tccagcgcag cttgcaaaaa atgagatcca agcctgcaac tggtgagcct
     5401 cagaaggggc aaccagagcc tgcaaaagac cagcaacaga aggcagctca acaccacaca
     5461 gaggaacaaa gcaaggtgcc cccagagcag aagagaaagg cgggctctac ctcatggacc
     5521 gggttccaga ccaaggctga agtggagcag gtggagctgc ctgatggaaa gaagagaaga
     5581 aaggtcctgg cgctcccctc acaccgaggc cccaaaatca ggttgcggga caaaggcaaa
     5641 gtgaagcccg tccatcccaa aaagccaaag ccacagataa accagtggaa gcaggagaag
     5701 cagcaattat cgtccgagca ggtatctagg aaaaaagcta agggaaataa gacggaaacc
     5761 cgcttcaacc agctggtcga acaatataag cagaaattat tgggaccttc taaaggagca
     5821 cctcttgcaa agaggagcaa atggtttgat agttgatacc cagctttctt gtacaaagtg
     5881 gtagtcatgt aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta
     5941 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt
     6001 atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc
     6061 gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag
     6121 gctttaattt gcggccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     6181 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     6241 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     6301 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat
     6361 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6421 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6481 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6541 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6601 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6661 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6721 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6781 taacgtttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     6841 ccgcataggc aagtgcacaa acaatactta aataaatact actcagtaat aacctatttc
     6901 ttagcatttt tgacgaaatt tgctattttg ttagagtctt ttacaccatt tgtctccaca
     6961 cctccgctta catcaacacc aataacgcca tttaatctaa gcgcatcacc aacattttct
     7021 ggcgtcagtc caccagctaa cataaaatgt aagctttcgg ggctctcttg ccttccaacc
     7081 cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc cacctgcttc tgaatcaaac
     7141 aagggaataa acgaatgagg tttctgtgaa gctgcactga gtagtatgtt gcagtctttt
     7201 ggaaatacga gtcttttaat aactggcaaa ccgaggaact cttggtattc ttgccacgac
     7261 tcatctccat gcagttggac gatatcaatg ccgtaatcat tgaccagagc caaaacatcc
     7321 tccttaggtt gattacgaaa cacgccaacc aagtatttcg gagtgcctga actattttta
     7381 tatgctttta caagacttga aattttcctt gcaataaccg ggtcaattgt tctctttcta
     7441 ttgggcacac atataatacc cagcaagtca gcatcggaat ctagagcaca ttctgcggcc
     7501 tctgtgctct gcaagccgca aactttcacc aatggaccag aactacctgt gaaattaata
     7561 acagacatac tccaagctgc ctttgtgtgc ttaatcacgt atactcacgt gctcaatagt
     7621 caccaatgcc ctccctcttg gccctctcct tttctttttt cgaccgaatt aattcttaat
     7681 cggcaaaaaa agaaaagctc cggatcaaga ttgtacgtaa ggtgacaagc tatttttcaa
     7741 taaagaatat cttccactac tgccatctgg cgtcataact gcaaagtaca catatattac
     7801 gatgctgtct attaaatgct tcctatatta tatatatagt aatgtcgttt atggtgcact
     7861 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     7921 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7981 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcga
//
