LOCUS       pcDNA5/FRT/TO_-_E5_Insert_GT>A        6752 bp    DNA     circular UNA 15-JUL-2019
DEFINITION  Restriction Cloning ligation product.
ACCESSION   urn.local...15-a7u3uag
VERSION     urn.local...15-a7u3uag
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          join(1..>988,<2662..6752)
                     /organism="pcDNA5/FRT/TO"
                     /mol_type="other DNA"
                     /label="source pcDNA5/FRT/TO"
     promoter        236..812
                     /label="CMV_immearly_promoter"
     misc_feature    315..602
                     /label="CAG_enhancer"
     primer_bind     743..762
                     /Sequence="ATGTCGTAACAACTCCGCCC"
                     /Hairpin_Tm="39.3"
                     /Tm="60.4"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=539
                     /modified_by="Carson J Bryant"
                     /label="CMV_seq"
     primer_bind     769..789
                     /Sequence="CGCAAATGGGCGGTAGGCGTG"
                     /Hairpin_Tm="52.4"
                     /Tm="68.1"
                     /Self_Dimer_Tm="3.5"
                     /%GC="66.7"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="CMV_fwd_primer"
     promoter        770..893
                     /label="CMV_promoter"
     promoter        782..955
                     /label="CMV2_promoter"
     misc_feature    820..859
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="tetO reg"
                     /Transferred_Similarity="100.00%"
                     /modified_by="Carson J Bryant"
                     /label="tetO reg (2X)"
                     /note="Geneious type: Regulatory Sequence"
     primer_bind     869..893
                     /Sequence="AGCTCGTTTAGTGAACCGTCAGATC"
                     /Hairpin_Tm="33.3"
                     /Tm="63.2"
                     /Self_Dimer_Tm="None"
                     /%GC="48.0"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="LNCX_primer"
     TSS             886
                     /created_by="Carson J Bryant"
                     /label="TSS +1"
     primer_bind     928..947
                     /Sequence="ATAGAAGACACCGGGACCGA"
                     /annotation_group="14978805611616654461fa97-724e-4d1e-ac2d
                     -88bb32666190urn:sequence:genbank:null"
                     /modified_by="Carson J Bryant"
                     /Hairpin_Tm="42.8"
                     /Tm="60.0"
                     /Self_Dimer_Tm="13.1"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=203
                     /label="p5_ins_seqF"
     Insert          <985..>2665
                     /created_by="Carson J Bryant"
                     /modified_by="Carson J Bryant"
                     /label="Insert"
     misc_feature    985..2665
                     /Source="referenceToDocument: urn:local:.:2a-a4pr60o"
                     /label="E5 Insert Restriction KpnI NotI Extended Primer
                     (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <985..989
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        985..988
                     /label="Ligation"
     source          <985..>989
                     /organism="pcDNA5/FRT/TO"
                     /mol_type="other DNA"
                     /label="source pcDNA5/FRT/TO"
     exon            990..1065
                     /created_by="Carson J Bryant"
                     /label="Exon 4"
     primer_bind     990..1014
                     /Sequence="TGTTCAGAAGATGACTTGAAGACAG"
                     /Hairpin_Tm="37.8"
                     /Tm="59.1"
                     /Self_Dimer_Tm="None"
                     /%GC="40.0"
                     /Extension="ACTGGGTACC"
                     /modified_by="Carson J Bryant"
                     /label="E5_KpnI_F+4"
     primer_bind     1265..1284
                     /Mismatches=0
                     /Extension="GATTTAGGTGACACTATAG"
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="11247530480857741d5faa83-2d77-4acd-b287
                     -b7fbcf47143f"
                     /Sequence="GCTCTCCCCAAGTCCAAGTC"
                     /created_by="primer3"
                     /label="RBM28_E5_seq"
     exon            1449..1541
                     /created_by="Carson J Bryant"
                     /modified_by="Carson J Bryant"
                     /label="Exon 5"
     primer_bind     complement(1509..1550)
                     /Sequence="GAAAGCTTTCTTTTATCTCTTTCATGTTCATGCCTTTGAGAG"
                     /created_by="Carson J Bryant"
                     /label="E5_QC_ovr_R"
     primer_bind     complement(1513..1531)
                     /Sequence="TTTCATGTTCATGCCTTTG"
                     /Hairpin_Tm="None"
                     /Tm="51.7"
                     /Self_Dimer_Tm="7.8"
                     /%GC="36.8"
                     /created_by="primer3"
                     /label="E5_QCNEB_R"
     primer_bind     1532..1557
                     /Sequence="GAGATAAAAGAAAGCTTTCTATACCC"
                     /Hairpin_Tm="46.2"
                     /Tm="55.8"
                     /Self_Dimer_Tm="35.6"
                     /%GC="34.6"
                     /created_by="primer3"
                     /label="E5_QCNEB_F"
     primer_bind     1534..1578
                     /Sequence="GATAAAAGAAAGCTTTCTATACCCATATCATTGACCCAGATATTT"
                     /created_by="Carson J Bryant"
                     /label="E5_QC_ovr_F"
     misc_feature    1542
                     /Original_Bases="GT"
                     /label="GT"
                     /note="Geneious type: Editing History Replacement"
     exon            2588..2659
                     /created_by="Carson J Bryant"
                     /label="Exon 6"
     primer_bind     complement(2631..2659)
                     /created_by="Carson J Bryant"
                     /Sequence="CTATAGCAGAAACAGACTGTGTATCTTTA"
                     /Hairpin_Tm="39.0"
                     /Tm="59.0"
                     /Self_Dimer_Tm="9.3"
                     /%GC="34.5"
                     /created_by="primer3"
                     /Extension="ACTGGCGGCCGC"
                     /modified_by="Carson J Bryant"
                     /label="E5_NotI_R+4"
     misc_feature    2660..>2665
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     source          <2660..>2665
                     /organism="pcDNA5/FRT/TO"
                     /mol_type="other DNA"
                     /label="source pcDNA5/FRT/TO"
     misc_feature    2662..988
                     /Source="referenceToDocument: urn:local:.:f-9y9qyq6"
                     /label="pcDNA5/FRT/TO (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        2662..2665
                     /label="Ligation"
     misc_feature    2667..2672
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI"
                     /note="Geneious type: restriction site"
     misc_feature    2667..2672
                     /Recognition_pattern="C^YCGRG"
                     /annotation_group="CYCGRG"
                     /label="AvaI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(2702..2721)
                     /Mismatches=0
                     /%GC="55.0"
                     /Hairpin_Tm="40.4"
                     /Self_Dimer_Tm="None"
                     /Tm="60.6"
                     /annotation_group="6636858041222046ccb3b5a-2cd8-4096-bf09-
                     3ae5782ae530"
                     /Sequence="TAGAAGGCACAGTCGAGGCT"
                     /created_by="primer3"
                     /label="BGH_R_2"
     primer_bind     complement(2704..2721)
                     /Sequence="TAGAAGGCACAGTCGAGG"
                     /Hairpin_Tm="None"
                     /Tm="56.5"
                     /Self_Dimer_Tm="None"
                     /%GC="55.6"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="BGH_rev_primer"
     terminator      2707..2934
                     /label="bGH_PA_terminator"
     primer_bind     complement(2726..2745)
                     /Sequence="GGCAAACAACAGATGGCTGG"
                     /Hairpin_Tm="46.6"
                     /Tm="60.0"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /Pair_Dimer_Tm="None"
                     /Product_Size=203
                     /annotation_group="14978805611616654461fa97-724e-4d1e-ac2d
                     -88bb32666190urn:sequence:genbank:null"
                     /modified_by="Carson J Bryant"
                     /label="p5_ins_seqR"
     polyA_signal    2800..2805
                     /created_by="Carson J Bryant"
                     /modified_by="Carson J Bryant"
                     /label="PolyA_signal"
     Gene            3218..3265
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="FRT"
                     /Transferred_Similarity="100.00%"
                     /label="FRT"
     gene            3285..4292
                     /gene="hygroB"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFGQGSSYTF*"
                     /label="hygroB"
     terminator      4428..4547
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /label="SV40_PA_terminator"
     primer_bind     4516..4535
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /Sequence="GTGGTTTGTCCAAACTCATC"
                     /Hairpin_Tm="48.9"
                     /Tm="55.0"
                     /Self_Dimer_Tm="19.2"
                     /%GC="45.0"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="EBV_rev_primer"
     primer_bind     complement(4591..4609)
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /Sequence="GGAAACAGCTATGACCATG"
                     /Hairpin_Tm="None"
                     /Tm="53.8"
                     /Self_Dimer_Tm="None"
                     /%GC="47.4"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="M13_reverse_primer"
     primer_bind     complement(4608..4630)
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /Sequence="AGCGGATAACAATTTCACACAGG"
                     /Hairpin_Tm="None"
                     /Tm="59.7"
                     /Self_Dimer_Tm="None"
                     /%GC="43.5"
                     /created_by="primer3"
                     /modified_by="Carson J Bryant"
                     /label="M13_pUC_rev_primer"
     promoter        complement(4644..4673)
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /label="lac_promoter"
     gene            complement(5756..6616)
                     /gene="Ampicillin"
                     /translation="MQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFAS
                     AALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADL
                     SQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWA
                     EDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACME
                     QQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
                     GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /label="Ampicillin"
     promoter        complement(6658..6686)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="AmpR_promoter"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacct gttcagaaga tgacttgaag acagtatttg
     1021 ctcaatttgg agctgtcctg gaagtaaata tccctaggaa accaggtact aataaacctt
     1081 cacttggtgt ggattacgtt agttaacata ttacccttga acaaacatat atgatatata
     1141 tgaagtattt ggcacagtgc ttcacatcta atgaaaaccc aaataatggt tttattcccc
     1201 tgatgatatt ttctgttgga tcagaattag ttttagaagg cgagttagtt tgagaaagtg
     1261 ttttgctctc cccaagtcca agtcagagta tactcagtaa gaagatgtaa gcaattgctt
     1321 tggggaaggg cctgacctgc tggcagaatt tgggccatct tgtgggcctg aattgcagta
     1381 atttacttct tagtattctg gctacctcct gctgcctctc acttctttct ttgtgcattt
     1441 gcacacagat gggaagatgc gcggttttgg ttttgttcag ttcaaaaacc tcctagaagc
     1501 aggtaaagct ctcaaaggca tgaacatgaa agagataaaa gaaagctttc tatacccata
     1561 tcattgaccc agatatttct ggtgttttgg tgaattagtg tagtaaccca ctttctgcat
     1621 tttcaaaaga tgtttgcatt attaagagtc tcattgctaa tacggtagct atttaggtga
     1681 aatactctag aacttgatct tggggttaaa cttgaaagaa cccttgccag tattctttct
     1741 tttatctggt gagagtgtcc ttttaaatcc ctagtgaaaa atgtccctga gataattgct
     1801 gatagatgca ggttggtatc tagattcctc cttggttata tcactgttca gcatctgtgg
     1861 gctatttatt aaactgactg tgatttatat attgacttat caagtactgt gcctaaagaa
     1921 gttattgccg gccaggtgcg gtggctcaca cctgtaatcc tagcactttg ggaggccgag
     1981 gaggcaggtg gatcacctga ggtcaggagt tcaagaccag cctggtcaac atggcgaaac
     2041 cccgtctcta ctgaaaatac aaaaattagc caggtgtggt ggtgcatgct tgtaatccca
     2101 gctactcaag aggctgaggc aggagaatcg cttgaacctg gaggcggagg ttgcagtgaa
     2161 ctgagatggc accactgcac tccagcctgg gcgacagagc gagactgtgt ctcaaaaaaa
     2221 aaaaaaagaa gttattgccg tagagatgtc ctagcttcca ggtagggtgc agatgattcc
     2281 agaatgcctg ttttgattcc tgatagtcta actggaaaca tagttctaat tctttatttt
     2341 tttactccat cttatcccag gtgtggtggc tatgtagaaa ggcaaaattt ctgttcattg
     2401 ataaaaaggt taagggttaa aaggagtagg cagtgactag gagctttatt ttaagaccag
     2461 cattccctga attggcagtt tctttgtctc cactggttcc attattgtct gtgatgacta
     2521 ggggtcattt acagaggtag ggtttttaaa aggagagcct ttctgatgcc acttttctgc
     2581 ttgacaggcc ggacagtggc tgtggattgg gccgtggcaa aggataaata taaagataca
     2641 cagtctgttt ctgctatagg cggccgctcg agtctagagg gcccgtttaa acccgctgat
     2701 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     2761 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     2821 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     2881 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     2941 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     3001 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3061 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3121 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3181 ccaaaaaact tgattagggt gatggttcac gtacctagaa gttcctattc cgaagttcct
     3241 attctctaga aagtatagga acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg
     3301 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     3361 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     3421 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     3481 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     3541 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     3601 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     3661 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     3721 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     3781 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     3841 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     3901 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     3961 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     4021 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     4081 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     4141 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     4201 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     4261 gacgccccag cactcgtccg agggcaaagg aatagcacgt actacgagat ttcgattcca
     4321 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     4381 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     4441 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4501 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     4561 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     4621 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     4681 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     4741 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4801 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4861 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4921 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4981 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5041 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5101 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5161 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5221 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5281 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5341 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5401 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     5461 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5521 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5581 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5641 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     5701 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5761 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5821 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5881 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5941 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6001 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6061 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6121 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6181 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6241 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6301 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6361 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6421 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6481 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6541 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6601 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6661 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6721 gcacatttcc ccgaaaagtg ccacctgacg tc
//
